Rh6AG301400

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003015
Physical Location & Seq
Reverse (-)
50008811 .. 50011532
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG301400.1

Sequence Viewer

Length: 831 bp
ATGTCGTTTGAGGACGAGGAGGAGTCGTTCGAGCACACGCTGCTGGTGGTGCGCGAGGTCGCCGTCTACAAAATCCCGCCGCGGTCGACGTCCGGCGGGTACAAGTGCGGCGAGTGGCTCCAGTCCGACAAGATCTGGACGGGTCGCCTCCGGGTCGTGTCCTGCAAGGACCGCTGCGAGATCCGGCTCGAGGATCCCAACTCCAGCGAGCTCTTCGCCGCCTGCTTCGTCCCGCCGGGGCAGCGCGAGACCTCCGTCGAGACCGTCCTCGACTCCTCCCGCTACTTCGTCCTCAAGATCGAGGACGGCGCCGGCAAGCACGCCTTCATCGGCCTCGGGTTCGCCGAGCGCAACGAGGCCTTCGATTTCAACGTCGCGCTCTCCGATCACGACAAGTACGTCAAGCGAGAGACTGATAATGACGTCGGCGGCGGCGGCGGCGACGACGACAGTCACATTGAGGTTCACCCGGCGGTGAATCACAGATTGAAGGAAGGTGAGACGATAAGGATAAATGTGAAGCCCAAGCCGACGAGTGGGACCGGGATGCTGTCAGCAGCTGGGCTATCCGGGTCGGCAAAGCCCAAATCGCCGGCTTTAAGCCTGGCCCCGCCACCCGGGTCCGGGAAACTCAGGTCACCTCTGCCGCCGCCTCCAAATGATCCTGTTGCTGCTAGGATCAGCTCTGGTGGTGTCAAACCTCCACAGGATAGTGCAAGAAAGAGCTCCGACCCGTTATCGGATCTTTCGCCAATTGAGAGGAATCTGCCTCCAGCAAATGGATCAGGATCATCCACAAAGACAAATGCAGTAGGATGGGCAGCTTTCTGA

Protein Analysis

276

Amino Acids

29.55

Weight (kDa)

5.76

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 10 - 174 1.8e-57 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AatII GACGTC 2 cut(s) 92, 426
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 2 cut(s) 66, 86
AccII CGCG 4 cut(s) 54, 82, 246, 377
AclWI GGATC 8 cut(s) 175, 188, 201, 656, 686, 750, 790, 796
AcyI GRCGYC 3 cut(s) 89, 309, 423
AfaI GTAC 2 cut(s) 101, 398
AfiI CCNNNNNNNGG 7 cut(s) 190, 536, 617, 623, 624, 739, 779
AgsI TTSAA 2 cut(s) 370, 490
AjnI CCWGG 1 cut(s) 603
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 5 cut(s) 211, 560, 684, 726, 824
AluI AGCT 5 cut(s) 211, 560, 684, 726, 824
Alw21I GWGCWC 3 cut(s) 36, 213, 728
Alw26I GTCTC 4 cut(s) 242, 254, 404, 494
AlwI GGATC 8 cut(s) 175, 188, 201, 656, 686, 750, 790, 796
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 3 cut(s) 188, 335, 617
AoxI GGCC 3 cut(s) 331, 357, 606
ApeKI GCWGC 6 cut(s) 40, 174, 241, 557, 671, 821
AspLEI GCGC 5 cut(s) 54, 246, 311, 351, 379
AspS9I GGNCC 4 cut(s) 169, 540, 607, 621
AsuC2I CCSGG 8 cut(s) 152, 237, 470, 544, 571, 618, 619, 625
AsuHPI GGTGA 4 cut(s) 458, 487, 509, 630
AvaI CYCGRG 3 cut(s) 188, 335, 617
AvaII GGWCC 3 cut(s) 169, 540, 621
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 2 cut(s) 213, 728
Bbv12I GWGCWC 3 cut(s) 36, 213, 728
BbvI GCAGC 5 cut(s) 27, 161, 253, 569, 658
BccI CCATC 1 cut(s) 810
BceAI ACGGC 2 cut(s) 47, 322
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 8 cut(s) 152, 237, 470, 544, 571, 618, 619, 625
BcoDI GTCTC 4 cut(s) 242, 254, 404, 494
BfaI CTAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 312
BglII AGATCT 1 cut(s) 132
Bme1390I CCNGG 9 cut(s) 152, 237, 470, 544, 571, 605, 618, 619, 625
Bme18I GGWCC 3 cut(s) 169, 540, 621
BmeT110I CYCGRG 3 cut(s) 188, 335, 617
BmgT120I GGNCC 4 cut(s) 169, 540, 607, 621
BmiI GGNNCC 6 cut(s) 119, 195, 310, 541, 609, 622
BmrFI CCNGG 9 cut(s) 152, 237, 470, 544, 571, 605, 618, 619, 625
BmsI GCATC 1 cut(s) 537
BoxI GACNNNNGTC 2 cut(s) 254, 450
BpmI CTGGAG 3 cut(s) 104, 187, 756
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 8 cut(s) 152, 237, 470, 544, 571, 618, 619, 625
BsaBI GATNNNNATC 1 cut(s) 787
BsaHI GRCGYC 3 cut(s) 89, 309, 423
BsaI GGTCTC 2 cut(s) 242, 254
BsaJI CCNNGG 4 cut(s) 80, 236, 334, 617
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 7 cut(s) 190, 536, 617, 623, 624, 739, 779
Bse118I RCCGGY 2 cut(s) 311, 592
Bse1I ACTGG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 787
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 4 cut(s) 80, 236, 334, 617
BseGI GGATG 3 cut(s) 552, 791, 821
BseJI GATNNNNATC 1 cut(s) 787
BseLI CCNNNNNNNGG 7 cut(s) 190, 536, 617, 623, 624, 739, 779
BseMII CTCAG 1 cut(s) 646
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 3 cut(s) 32, 35, 265
BseXI GCAGC 5 cut(s) 27, 161, 253, 569, 658
BseYI CCCAGC 1 cut(s) 560
Bsh1236I CGCG 4 cut(s) 54, 82, 246, 377
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 333, 359, 608
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 3 cut(s) 36, 213, 728
BsiHKCI CYCGRG 3 cut(s) 188, 335, 617
BslFI GGGAC 2 cut(s) 215, 553
BslI CCNNNNNNNGG 7 cut(s) 190, 536, 617, 623, 624, 739, 779
BsmAI GTCTC 4 cut(s) 242, 254, 404, 494
BsmBI CGTCTC 1 cut(s) 494
BsmFI GGGAC 2 cut(s) 215, 553
BsnI GGCC 3 cut(s) 333, 359, 608
Bso31I GGTCTC 2 cut(s) 242, 254
BsoBI CYCGRG 3 cut(s) 188, 335, 617
Bsp1286I GDGCHC 3 cut(s) 36, 213, 728
BspANI GGCC 3 cut(s) 333, 359, 608
BspCNI CTCAG 1 cut(s) 645
BspFNI CGCG 4 cut(s) 54, 82, 246, 377
BspLI GGNNCC 6 cut(s) 119, 195, 310, 541, 609, 622
BspPI GGATC 8 cut(s) 175, 188, 201, 656, 686, 750, 790, 796
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 242, 254
BsrFI RCCGGY 2 cut(s) 311, 592
BsrI ACTGG 1 cut(s) 121
BssAI RCCGGY 2 cut(s) 311, 592
BssECI CCNNGG 4 cut(s) 80, 236, 334, 617
BssNI GRCGYC 3 cut(s) 89, 309, 423
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 265, 452
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 3 cut(s) 89, 309, 423
BstAPI GCANNNNNTGC 1 cut(s) 40
BstC8I GCNNGC 6 cut(s) 209, 223, 313, 317, 321, 594
BstDEI CTNAG 1 cut(s) 632
BstDSI CCRYGG 1 cut(s) 80
BstEII GGTNACC 1 cut(s) 636
BstF5I GGATG 3 cut(s) 552, 791, 821
BstFNI CGCG 4 cut(s) 54, 82, 246, 377
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 5 cut(s) 54, 246, 311, 351, 379
BstMAI GTCTC 4 cut(s) 242, 254, 404, 494
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 7 cut(s) 40, 49, 171, 241, 435, 438, 589
BstNI CCWGG 1 cut(s) 605
BstPAI GACNNNNGTC 2 cut(s) 254, 450
BstPI GGTNACC 1 cut(s) 636
BstSCI CCNGG 9 cut(s) 150, 235, 468, 542, 569, 603, 616, 617, 623
BstUI CGCG 4 cut(s) 54, 82, 246, 377
BstV1I GCAGC 5 cut(s) 27, 161, 253, 569, 658
BstX2I RGATCY 4 cut(s) 132, 180, 193, 742
BstYI RGATCY 4 cut(s) 132, 180, 193, 742
BsuRI GGCC 3 cut(s) 333, 359, 608
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 3 cut(s) 552, 791, 821
Cac8I GCNNGC 6 cut(s) 209, 223, 313, 317, 321, 594
CaiI CAGNNNCTG 1 cut(s) 560
CfoI GCGC 5 cut(s) 54, 246, 311, 351, 379
Cfr10I RCCGGY 2 cut(s) 311, 592
Cfr13I GGNCC 4 cut(s) 169, 540, 607, 621
Cfr42I CCGCGG 1 cut(s) 83
Cfr9I CCCGGG 1 cut(s) 617
Csp6I GTAC 2 cut(s) 100, 397
CviQI GTAC 2 cut(s) 100, 397
DdeI CTNAG 1 cut(s) 632
DinI GGCGCC 1 cut(s) 310
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Ecl136II GAGCTC 2 cut(s) 211, 726
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 213, 728
Eco31I GGTCTC 2 cut(s) 242, 254
Eco47I GGWCC 3 cut(s) 169, 540, 621
Eco53kI GAGCTC 2 cut(s) 211, 726
Eco88I CYCGRG 3 cut(s) 188, 335, 617
Eco91I GGTNACC 1 cut(s) 636
EcoICRI GAGCTC 2 cut(s) 211, 726
EcoO65I GGTNACC 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 603
EcoT38I GRGCYC 2 cut(s) 213, 728
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 494
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 2 cut(s) 215, 553
FauI CCCGC 5 cut(s) 84, 89, 240, 287, 618
FblI GTMKAC 2 cut(s) 66, 86
FokI GGATG 2 cut(s) 559, 778
FriOI GRGCYC 2 cut(s) 213, 728
FspBI CTAG 1 cut(s) 675
GlaI GCGC 5 cut(s) 53, 245, 310, 350, 378
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 3 cut(s) 104, 187, 756
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 3 cut(s) 333, 359, 608
HhaI GCGC 5 cut(s) 54, 246, 311, 351, 379
Hin1I GRCGYC 3 cut(s) 89, 309, 423
Hin6I GCGC 5 cut(s) 52, 244, 309, 349, 377
HinP1I GCGC 5 cut(s) 52, 244, 309, 349, 377
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 4 cut(s) 23, 272, 478, 763
HphI GGTGA 4 cut(s) 458, 487, 509, 630
Hpy166II GTNNAC 3 cut(s) 67, 87, 466
Hpy188I TCNGA 5 cut(s) 127, 385, 730, 742, 830
Hpy188III TCNNGA 5 cut(s) 136, 259, 295, 389, 786
Hpy8I GTNNAC 3 cut(s) 67, 87, 466
Hpy99I CGWCG 7 cut(s) 91, 260, 377, 428, 446, 449, 535
HpyAV CCTTC 4 cut(s) 334, 370, 484, 488
HpyCH4III ACNGT 2 cut(s) 265, 452
HpyCH4IV ACGT 4 cut(s) 89, 372, 399, 423
HpyCH4V TGCA 3 cut(s) 165, 716, 809
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 49, 171, 241, 435, 438, 589
HpyF3I CTNAG 1 cut(s) 632
HpySE526I ACGT 4 cut(s) 89, 372, 399, 423
Hsp92I GRCGYC 3 cut(s) 89, 309, 423
HspAI GCGC 5 cut(s) 52, 244, 309, 349, 377
KasI GGCGCC 1 cut(s) 308
KroI GCCGGC 2 cut(s) 311, 592
KroNI GCCGGC 2 cut(s) 313, 594
KspI CCGCGG 1 cut(s) 83
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 123, 731
Lsp1109I GCAGC 5 cut(s) 27, 161, 253, 569, 658
LweI GCATC 1 cut(s) 537
MaeI CTAG 1 cut(s) 675
MaeII ACGT 4 cut(s) 89, 372, 399, 423
MaeIII GTNAC 2 cut(s) 452, 636
MboII GAAGA 1 cut(s) 205
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 4 cut(s) 132, 180, 193, 742
MhlI GDGCHC 3 cut(s) 36, 213, 728
MluCI AATT 1 cut(s) 753
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 2 cut(s) 32, 266
MmeI TCCRAC 2 cut(s) 150, 753
MroNI GCCGGC 2 cut(s) 311, 592
MseI TTAA 1 cut(s) 599
MspA1I CMGCKG 3 cut(s) 82, 174, 560
MspR9I CCNGG 9 cut(s) 152, 237, 470, 544, 571, 605, 618, 619, 625
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 4 cut(s) 54, 82, 246, 377
MwoI GCNNNNNNNGC 7 cut(s) 40, 49, 171, 241, 435, 438, 589
NaeI GCCGGC 2 cut(s) 313, 594
NarI GGCGCC 1 cut(s) 309
NciI CCSGG 8 cut(s) 152, 237, 470, 544, 571, 618, 619, 625
NgoMIV GCCGGC 2 cut(s) 311, 592
NlaIV GGNNCC 6 cut(s) 119, 195, 310, 541, 609, 622
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 452, 636
PaeR7I CTCGAG 1 cut(s) 188
PceI AGGCCT 1 cut(s) 359
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 5 cut(s) 342, 360, 369, 381, 396
PdiI GCCGGC 2 cut(s) 313, 594
PfeI GAWTC 2 cut(s) 478, 763
PflMI CCANNNNNTGG 1 cut(s) 779
PfoI TCCNGGA 1 cut(s) 623
PleI GAGTC 2 cut(s) 31, 266
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 2 cut(s) 31, 266
PshAI GACNNNNGTC 2 cut(s) 254, 450
Psp124BI GAGCTC 2 cut(s) 213, 728
Psp6I CCWGG 1 cut(s) 603
PspEI GGTNACC 1 cut(s) 636
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 6 cut(s) 119, 195, 310, 541, 609, 622
PspPI GGNCC 4 cut(s) 169, 540, 607, 621
PspXI VCTCGAGB 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 4 cut(s) 132, 180, 193, 742
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 101, 398
RsaNI GTAC 2 cut(s) 100, 397
SacI GAGCTC 2 cut(s) 213, 728
SacII CCGCGG 1 cut(s) 83
SalI GTCGAC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 599
Sau96I GGNCC 4 cut(s) 169, 540, 607, 621
SchI GAGTC 2 cut(s) 32, 266
ScrFI CCNGG 9 cut(s) 152, 237, 470, 544, 571, 605, 618, 619, 625
SduI GDGCHC 3 cut(s) 36, 213, 728
SfaNI GCATC 1 cut(s) 537
SfoI GGCGCC 1 cut(s) 310
Sfr274I CTCGAG 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 3 cut(s) 169, 540, 621
SlaI CTCGAG 1 cut(s) 188
SmaI CCCGGG 1 cut(s) 619
SmlI CTYRAG 2 cut(s) 188, 293
SmoI CTYRAG 2 cut(s) 188, 293
Sse9I AATT 1 cut(s) 753
SseBI AGGCCT 1 cut(s) 359
SspDI GGCGCC 1 cut(s) 308
SspMI CTAG 1 cut(s) 675
SstI GAGCTC 2 cut(s) 213, 728
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 9 cut(s) 150, 235, 468, 542, 569, 603, 616, 617, 623
TaaI ACNGT 2 cut(s) 265, 452
TaiI ACGT 4 cut(s) 92, 375, 402, 426
TaqI TCGA 7 cut(s) 30, 86, 189, 258, 270, 300, 363
TasI AATT 1 cut(s) 753
TauI GCSGC 9 cut(s) 82, 111, 221, 432, 435, 438, 441, 649, 652
TfiI GAWTC 2 cut(s) 478, 763
Tru1I TTAA 1 cut(s) 599
Tru9I TTAA 1 cut(s) 599
TseFI GTSAC 2 cut(s) 452, 636
TseI GCWGC 6 cut(s) 40, 174, 241, 557, 671, 821
Tsp45I GTSAC 2 cut(s) 452, 636
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 1 cut(s) 244
TspMI CCCGGG 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 779
VpaK11BI GGWCC 3 cut(s) 169, 540, 621
XhoI CTCGAG 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 617
XmiI GTMKAC 2 cut(s) 66, 86
XspI CTAG 1 cut(s) 675
ZraI GACGTC 2 cut(s) 90, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.