pycom05g18200

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003015
Physical Location & Seq
Reverse (-)
21182174 .. 21184450
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18200.1

Sequence Viewer

Length: 1260 bp
ATGAAAAATGAAATTATCGATAGGCGGATTTCTCGTAATTTGTTGATTTTGTGTAGCAATTTAGGTTCGAATCGACACAACAACAACAAAAAATTAAAAAAAATTGAGAGTGGGATAAAGGGGCAAGATGGTCATATCACGTCACCTCCATTGGACAAATTCCAGTTGACCTTTCAAGCAAACTGCCTCACCTTCGAACCCACGTCTCCGCAGACTCCCTCATTTGAAACCTTCCCCCCACCATTTTCCCATTTCTTCAAATTCACATCAAATTCTCCCCAATTTCAAGTCCCCAACTCCTTCACCAAAAATTTGATCCGAAAAAAAAAATCAAAATTTCGTAATTTTCTCGAATTATCAGCCGCATTGCGCGATCAGAATCCGGAAAAGCTCGAAGCTTTCTGCCCCCAATTCGATCCCGAAACGATGTCGTTCGAGGAGGAGGAGGAGGAGGCATTCGAGCACACGCTTCTCGTGGTCCGCGAGGTCGCGGTCTTCAAAATTCCGCCGCGGAGCACTTCCGGCGGGTACAAGTGCGGCGAGTGGCTCCAGTCCGATAAGATCTGGTCGGGTCGGCTCCGGGTCGTGTCGTGCAAGGACCGGTGCGAGATCCGGCTGGAGGATCCGAACTCCAGCGAGCTGTTCGCCGCCTGCTTCGTCCACCCGGGCCAGCGCGAGACCTCCGTCGAGACCGTGCTCGACTCGTCGCGCTACTTCGTGCTCAAGATCGAGGACGGCACCGGGAAGCACGCCTTCATCGGACTAGGATTCGCGGAGCGGAACGAGGCGTTCGATTTCAACGTGGCGCTTTCGGACCACGATAAGTACGTGAAGAGGGAGCACGAGAAGGAGAGCAACGGCGGCGAAACCAGCGACGACTGCCACATCGATATTCATCCCGCCGTCAATCACAGATTGAAAGAAGGTGAGACGATAAGGATAAATGTGAAGCCGAAGCCGAAGCCGACGAGTGGGGCCGGGATGTTGTCCTCAGCCGGGCTATCCGGGACGGCCAAGGCGAAACCGATTAGTTTGGGGTTGGCTCCGCCGCCGGGGTCCGGGAAAGTTAGGCCGGCTCTGCCACCACCTCCCAATGACCCTGCTGCTGCTAGAATCAGCTCTGGTGGTGTTAAGTCGCCGGATAACACAAGACGTGGATCCGACCCGTTATCGGATCTTTCCCAGATTGAGAGGAGTCTTCCTCCAGCGACCGGATCAGCGTCGGCAAAGACAAGTGCAGCAGGATGGGCAGCCTTCTGA

Protein Analysis

420

Amino Acids

46.2

Weight (kDa)

8.51

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 153 - 317 2.3e-57 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 778
AccII CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
AccIII TCCGGA 1 cut(s) 382
AclWI GGATC 9 cut(s) 310, 410, 604, 617, 630, 1152, 1165, 1182, 1222
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 6 cut(s) 158, 260, 271, 310, 335, 501
AfaI GTAC 2 cut(s) 530, 827
AfiI CCNNNNNNNGG 7 cut(s) 619, 971, 996, 1052, 1058, 1171, 1211
AgeI ACCGGT 1 cut(s) 600
AgsI TTSAA 7 cut(s) 176, 227, 259, 287, 499, 799, 919
AjiI CACGTC 3 cut(s) 141, 204, 1154
AluBI AGCT 4 cut(s) 391, 398, 640, 1119
AluI AGCT 4 cut(s) 391, 398, 640, 1119
Alw21I GWGCWC 5 cut(s) 465, 518, 699, 723, 843
Alw26I GTCTC 4 cut(s) 210, 671, 683, 923
AlwI GGATC 9 cut(s) 310, 410, 604, 617, 630, 1152, 1165, 1182, 1222
Ama87I CYCGRG 1 cut(s) 664
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 4 cut(s) 667, 975, 1011, 1070
ApeKI GCWGC 4 cut(s) 1103, 1106, 1238, 1250
ApoI RAATTY 6 cut(s) 158, 260, 271, 310, 335, 501
AsiGI ACCGGT 1 cut(s) 600
AspLEI GCGC 4 cut(s) 372, 675, 711, 808
AspS9I GGNCC 6 cut(s) 478, 598, 667, 814, 975, 1056
AsuC2I CCSGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
AsuHPI GGTGA 4 cut(s) 135, 181, 295, 938
AsuII TTCGAA 2 cut(s) 68, 195
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 4 cut(s) 478, 598, 814, 1056
BamHI GGATCC 2 cut(s) 622, 1157
BanI GGYRCC 1 cut(s) 737
BauI CACGAG 2 cut(s) 473, 842
BbsI GAAGAC 2 cut(s) 487, 1190
Bbv12I GWGCWC 5 cut(s) 465, 518, 699, 723, 843
BbvCI CCTCAGC 1 cut(s) 991
BbvI GCAGC 3 cut(s) 1090, 1093, 1250
BccI CCATC 2 cut(s) 122, 1239
BceAI ACGGC 4 cut(s) 751, 874, 887, 1026
BcnI CCSGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
BcoDI GTCTC 4 cut(s) 210, 671, 683, 923
BfaI CTAG 2 cut(s) 764, 1110
BfoI RGCGCY 1 cut(s) 809
BglII AGATCT 1 cut(s) 561
Bme1390I CCNGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
Bme18I GGWCC 4 cut(s) 478, 598, 814, 1056
BmeT110I CYCGRG 1 cut(s) 664
BmgBI CACGTC 3 cut(s) 141, 204, 1154
BmgT120I GGNCC 6 cut(s) 478, 598, 667, 814, 975, 1056
BmiI GGNNCC 8 cut(s) 548, 578, 624, 739, 976, 1044, 1057, 1159
BmrFI CCNGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
BoxI GACNNNNGTC 1 cut(s) 683
BpiI GAAGAC 2 cut(s) 487, 1190
BplI GAGNNNNNCTC 2 cut(s) 1186, 1218
BpmI CTGGAG 4 cut(s) 533, 616, 638, 1188
Bpu10I CCTNAGC 1 cut(s) 991
Bpu14I TTCGAA 2 cut(s) 68, 195
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
Bsa29I ATCGAT 2 cut(s) 18, 888
BsaAI YACGTR 1 cut(s) 829
BsaBI GATNNNNATC 2 cut(s) 378, 894
BsaI GGTCTC 2 cut(s) 671, 683
BsaJI CCNNGG 4 cut(s) 509, 664, 1014, 1052
BsaWI WCCGGW 3 cut(s) 382, 600, 1211
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 7 cut(s) 619, 971, 996, 1052, 1058, 1171, 1211
Bse118I RCCGGY 2 cut(s) 600, 1072
Bse1I ACTGG 2 cut(s) 163, 550
Bse3DI GCAATG 1 cut(s) 365
Bse8I GATNNNNATC 2 cut(s) 378, 894
BseAI TCCGGA 1 cut(s) 382
BseCI ATCGAT 2 cut(s) 18, 888
BseDI CCNNGG 4 cut(s) 509, 664, 1014, 1052
BseGI GGATG 3 cut(s) 895, 987, 1250
BseJI GATNNNNATC 2 cut(s) 378, 894
BseLI CCNNNNNNNGG 7 cut(s) 619, 971, 996, 1052, 1058, 1171, 1211
BseMI GCAATG 1 cut(s) 365
BseMII CTCAG 1 cut(s) 1005
BseNI ACTGG 2 cut(s) 163, 550
BseRI GAGGAG 6 cut(s) 452, 455, 458, 461, 464, 1207
BseXI GCAGC 3 cut(s) 1090, 1093, 1250
BsgI GTGCAG 1 cut(s) 1257
Bsh1236I CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
Bsh1285I CGRYCG 1 cut(s) 1212
BshFI GGCC 4 cut(s) 669, 977, 1013, 1072
BshNI GGYRCC 1 cut(s) 737
BshTI ACCGGT 1 cut(s) 600
BshVI ATCGAT 2 cut(s) 18, 888
BsiEI CGRYCG 1 cut(s) 1212
BsiHKAI GWGCWC 5 cut(s) 465, 518, 699, 723, 843
BsiHKCI CYCGRG 1 cut(s) 664
BslFI GGGAC 2 cut(s) 275, 1021
BslI CCNNNNNNNGG 7 cut(s) 619, 971, 996, 1052, 1058, 1171, 1211
BsmAI GTCTC 4 cut(s) 210, 671, 683, 923
BsmBI CGTCTC 2 cut(s) 210, 923
BsmFI GGGAC 2 cut(s) 275, 1021
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 4 cut(s) 669, 977, 1013, 1072
Bso31I GGTCTC 2 cut(s) 671, 683
BsoBI CYCGRG 1 cut(s) 664
Bsp119I TTCGAA 2 cut(s) 68, 195
Bsp1286I GDGCHC 5 cut(s) 465, 518, 699, 723, 843
Bsp13I TCCGGA 1 cut(s) 382
BspANI GGCC 4 cut(s) 669, 977, 1013, 1072
BspCNI CTCAG 1 cut(s) 1004
BspDI ATCGAT 2 cut(s) 18, 888
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
BspLI GGNNCC 8 cut(s) 548, 578, 624, 739, 976, 1044, 1057, 1159
BspPI GGATC 9 cut(s) 310, 410, 604, 617, 630, 1152, 1165, 1182, 1222
BspT104I TTCGAA 2 cut(s) 68, 195
BspT107I GGYRCC 1 cut(s) 737
BspTNI GGTCTC 2 cut(s) 671, 683
BsrBI CCGCTC 1 cut(s) 778
BsrDI GCAATG 1 cut(s) 365
BsrFI RCCGGY 2 cut(s) 600, 1072
BsrI ACTGG 2 cut(s) 163, 550
BssAI RCCGGY 2 cut(s) 600, 1072
BssECI CCNNGG 4 cut(s) 509, 664, 1014, 1052
BssSI CACGAG 2 cut(s) 473, 842
BssT1I CCWWGG 1 cut(s) 1014
Bst2BI CACGAG 2 cut(s) 473, 842
Bst4CI ACNGT 1 cut(s) 694
Bst6I CTCTTC 1 cut(s) 827
BstBAI YACGTR 1 cut(s) 829
BstBI TTCGAA 2 cut(s) 68, 195
BstC8I GCNNGC 5 cut(s) 638, 652, 671, 750, 1074
BstDEI CTNAG 1 cut(s) 991
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 3 cut(s) 895, 987, 1250
BstFNI CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
BstH2I RGCGCY 1 cut(s) 809
BstHHI GCGC 4 cut(s) 372, 675, 711, 808
BstMAI GTCTC 4 cut(s) 210, 671, 683, 923
BstMCI CGRYCG 1 cut(s) 1212
BstMWI GCNNNNNNNGC 6 cut(s) 522, 861, 870, 879, 1078, 1247
BstPAI GACNNNNGTC 1 cut(s) 683
BstSCI CCNGG 9 cut(s) 579, 663, 664, 740, 977, 995, 1004, 1051, 1058
BstUI CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
BstV1I GCAGC 3 cut(s) 1090, 1093, 1250
BstV2I GAAGAC 2 cut(s) 487, 1190
BstX2I RGATCY 5 cut(s) 561, 609, 622, 1157, 1174
BstYI RGATCY 5 cut(s) 561, 609, 622, 1157, 1174
Bsu15I ATCGAT 2 cut(s) 18, 888
BsuRI GGCC 4 cut(s) 669, 977, 1013, 1072
BsuTUI ATCGAT 2 cut(s) 18, 888
BtgI CCRYGG 1 cut(s) 509
BtrI CACGTC 3 cut(s) 141, 204, 1154
BtsCI GGATG 3 cut(s) 895, 987, 1250
Cac8I GCNNGC 5 cut(s) 638, 652, 671, 750, 1074
CfoI GCGC 4 cut(s) 372, 675, 711, 808
Cfr10I RCCGGY 2 cut(s) 600, 1072
Cfr13I GGNCC 6 cut(s) 478, 598, 667, 814, 975, 1056
Cfr42I CCGCGG 1 cut(s) 512
Cfr9I CCCGGG 1 cut(s) 664
ClaI ATCGAT 2 cut(s) 18, 888
CseI GACGC 1 cut(s) 1209
Csp6I GTAC 2 cut(s) 529, 826
CspAI ACCGGT 1 cut(s) 600
CviQI GTAC 2 cut(s) 529, 826
DdeI CTNAG 1 cut(s) 991
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
EciI GGCGGA 3 cut(s) 40, 495, 1035
Eco130I CCWWGG 1 cut(s) 1014
Eco31I GGTCTC 2 cut(s) 671, 683
Eco47I GGWCC 4 cut(s) 478, 598, 814, 1056
Eco88I CYCGRG 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 2 cut(s) 210, 923
FaiI YATR 1 cut(s) 135
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FaqI GGGAC 2 cut(s) 275, 1021
FauI CCCGC 2 cut(s) 518, 907
FokI GGATG 2 cut(s) 882, 994
FspBI CTAG 2 cut(s) 764, 1110
GlaI GCGC 4 cut(s) 371, 674, 710, 807
GsuI CTGGAG 4 cut(s) 533, 616, 638, 1188
HaeII RGCGCY 1 cut(s) 809
HaeIII GGCC 4 cut(s) 669, 977, 1013, 1072
HgaI GACGC 1 cut(s) 1209
HhaI GCGC 4 cut(s) 372, 675, 711, 808
Hin6I GCGC 4 cut(s) 370, 673, 709, 806
HinP1I GCGC 4 cut(s) 370, 673, 709, 806
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 7 cut(s) 70, 214, 379, 701, 768, 1113, 1195
HphI GGTGA 4 cut(s) 135, 181, 295, 938
Hpy166II GTNNAC 2 cut(s) 168, 661
Hpy188I TCNGA 9 cut(s) 320, 378, 556, 627, 761, 814, 1162, 1174, 1259
Hpy188III TCNNGA 5 cut(s) 350, 383, 419, 688, 724
Hpy8I GTNNAC 2 cut(s) 168, 661
Hpy99I CGWCG 5 cut(s) 689, 709, 878, 970, 1225
HpyAV CCTTC 6 cut(s) 202, 241, 310, 763, 841, 917
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 5 cut(s) 140, 203, 801, 828, 1153
HpyCH4V TGCA 2 cut(s) 594, 1238
HpyF10VI GCNNNNNNNGC 6 cut(s) 522, 861, 870, 879, 1078, 1247
HpyF3I CTNAG 1 cut(s) 991
HpySE526I ACGT 5 cut(s) 140, 203, 801, 828, 1153
HspAI GCGC 4 cut(s) 370, 673, 709, 806
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 1072
KroNI GCCGGC 1 cut(s) 1074
KspI CCGCGG 1 cut(s) 512
LmnI GCTCC 6 cut(s) 513, 552, 582, 775, 838, 1048
Lsp1109I GCAGC 3 cut(s) 1090, 1093, 1250
MaeI CTAG 2 cut(s) 764, 1110
MaeII ACGT 5 cut(s) 140, 203, 801, 828, 1153
MaeIII GTNAC 1 cut(s) 141
MbiI CCGCTC 1 cut(s) 778
MboII GAAGA 4 cut(s) 247, 487, 844, 1190
MflI RGATCY 5 cut(s) 561, 609, 622, 1157, 1174
MhlI GDGCHC 5 cut(s) 465, 518, 699, 723, 843
MlyI GAGTC 3 cut(s) 208, 695, 1204
MmeI TCCRAC 1 cut(s) 1185
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 1072
MseI TTAA 2 cut(s) 95, 1131
MspA1I CMGCKG 1 cut(s) 511
MspR9I CCNGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
Mva1269I GAATGC 1 cut(s) 455
MvnI CGCG 7 cut(s) 372, 483, 491, 511, 675, 709, 773
MwoI GCNNNNNNNGC 6 cut(s) 522, 861, 870, 879, 1078, 1247
NaeI GCCGGC 1 cut(s) 1074
NciI CCSGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
NgoMIV GCCGGC 1 cut(s) 1072
NlaIV GGNNCC 8 cut(s) 548, 578, 624, 739, 976, 1044, 1057, 1159
NmuCI GTSAC 1 cut(s) 141
NspV TTCGAA 2 cut(s) 68, 195
PcsI WCGNNNNNNNCGW 5 cut(s) 480, 789, 798, 825, 1016
PctI GAATGC 1 cut(s) 455
PdiI GCCGGC 1 cut(s) 1074
PfeI GAWTC 4 cut(s) 70, 379, 768, 1113
PfoI TCCNGGA 2 cut(s) 1004, 1058
PinAI ACCGGT 1 cut(s) 600
PleI GAGTC 3 cut(s) 208, 695, 1203
PpsI GAGTC 3 cut(s) 208, 695, 1203
Ppu21I YACGTR 1 cut(s) 829
PshAI GACNNNNGTC 1 cut(s) 683
PspN4I GGNNCC 8 cut(s) 548, 578, 624, 739, 976, 1044, 1057, 1159
PspPI GGNCC 6 cut(s) 478, 598, 667, 814, 975, 1056
PsuI RGATCY 5 cut(s) 561, 609, 622, 1157, 1174
RsaI GTAC 2 cut(s) 530, 827
RsaNI GTAC 2 cut(s) 529, 826
SacII CCGCGG 1 cut(s) 512
SaqAI TTAA 2 cut(s) 95, 1131
Sau96I GGNCC 6 cut(s) 478, 598, 667, 814, 975, 1056
SchI GAGTC 3 cut(s) 208, 695, 1204
ScrFI CCNGG 9 cut(s) 581, 665, 666, 742, 979, 997, 1006, 1053, 1060
SduI GDGCHC 5 cut(s) 465, 518, 699, 723, 843
Sfr303I CCGCGG 1 cut(s) 512
SfuI TTCGAA 2 cut(s) 68, 195
SgrBI CCGCGG 1 cut(s) 512
SinI GGWCC 4 cut(s) 478, 598, 814, 1056
SmaI CCCGGG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SspMI CTAG 2 cut(s) 764, 1110
StyD4I CCNGG 9 cut(s) 579, 663, 664, 740, 977, 995, 1004, 1051, 1058
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 5 cut(s) 143, 206, 804, 831, 1156
TauI GCSGC 6 cut(s) 365, 511, 540, 650, 864, 1051
TfiI GAWTC 4 cut(s) 70, 379, 768, 1113
Tru1I TTAA 2 cut(s) 95, 1131
Tru9I TTAA 2 cut(s) 95, 1131
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 4 cut(s) 1103, 1106, 1238, 1250
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 17, 24, 745, 884
TspGWI ACGGA 1 cut(s) 673
TspMI CCCGGG 1 cut(s) 664
VpaK11BI GGWCC 4 cut(s) 478, 598, 814, 1056
XapI RAATTY 6 cut(s) 158, 260, 271, 310, 335, 501
XmaI CCCGGG 1 cut(s) 664
XspI CTAG 2 cut(s) 764, 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.