pycom10g15730

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003015
Physical Location & Seq
Reverse (-)
18922214 .. 18923970
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g15730.1

Sequence Viewer

Length: 825 bp
ATGTCGTTCGACGAGGAGGAGGAGGCGTTCGAGCACACGCTGCTCGTGGTCCGGGAGGTCGCAGTCTTCAAAATCCCGCCGCGGAGCACTTCCGGCGGATACAAGTGCGGCGAGTGGCTCCAGTCTGATAAGATCTGGTCCGGTCGGCTCCGGGTCGTGTCATGCAAGGACCGGTGCGAGATCCGGCTGGAGGATCCGAACTCCAGCGAGCTCTTTGCCGCCTGCTTTGTCCACCCGGGCCAGCGCGAGACCTCAGTCGAGACCGTCCTCGACTCGTCGCGCTACTTCGTACTCAAGATCGAGGACGGCACCGGGAAGCACGCCTTCATCGGGCTAGGGTTCGCGGAGCGGAACGAAGCGTTCGATTTCAACGTGGCGCTTTCGGACCACGAGAAGTACGTGAAGAGGGAGCACGAGAAGGAGAGCAACGGCGGCGAAACCAGCGACGAGGGCCACATCGATATTCATCCCGCCCTCAATCATAGATTGAAAGAAGGTGAAACGATAAGGATAAATGTGAAGCCGAAGCCGACGAGTGGGGCCGGGATGCTGTCCGCCGCCGGGCTATCCGGGACGGCGAAGGCAAAACCTATTAGTTTGGGGTTGGCTCCGCCACCTGGGTCCGGGAAAGTTAGGTCGGCTCTGCCACCACCTCCCAATGACCCTGTTGCTGCTAGAATCAGCTCTGGTGTTGTCAAGTCGCCGGATAACACAAGACGTGGATCGGACCCGTTATCGGATCTTTCCCAGATTGAGAGGAATCTTCCCCCCGCGACTGGATCAGGCTCGACAAAGACAAGTGCAGCAGGATGGGCGGCTTTCTGA

Protein Analysis

275

Amino Acids

29.56

Weight (kDa)

6.1

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 10 - 174 2.6e-58 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 349
AccII CGCG 5 cut(s) 82, 246, 280, 344, 773
AclWI GGATC 6 cut(s) 175, 188, 201, 730, 747, 787
AfaI GTAC 2 cut(s) 291, 398
AfiI CCNNNNNNNGG 8 cut(s) 190, 330, 536, 561, 617, 623, 736, 776
AgeI ACCGGT 1 cut(s) 171
AgsI TTSAA 3 cut(s) 70, 370, 490
AjiI CACGTC 1 cut(s) 719
AjnI CCWGG 1 cut(s) 616
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 2 cut(s) 211, 684
AluI AGCT 2 cut(s) 211, 684
Alw21I GWGCWC 4 cut(s) 36, 89, 213, 414
Alw26I GTCTC 2 cut(s) 242, 254
AlwI GGATC 6 cut(s) 175, 188, 201, 730, 747, 787
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 3 cut(s) 238, 451, 540
ApeKI GCWGC 3 cut(s) 40, 671, 803
AsiGI ACCGGT 1 cut(s) 171
AspLEI GCGC 3 cut(s) 246, 282, 379
AspS9I GGNCC 9 cut(s) 49, 138, 169, 238, 385, 451, 540, 621, 727
AsuC2I CCSGG 9 cut(s) 53, 152, 236, 237, 313, 544, 562, 571, 625
AsuHPI GGTGA 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 235
AvaII GGWCC 6 cut(s) 49, 138, 169, 385, 621, 727
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 3 cut(s) 44, 389, 413
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 4 cut(s) 36, 89, 213, 414
BbvI GCAGC 3 cut(s) 27, 658, 815
BccI CCATC 1 cut(s) 804
BceAI ACGGC 3 cut(s) 322, 445, 591
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 618
BciVI GTATCC 1 cut(s) 92
BcnI CCSGG 9 cut(s) 53, 152, 236, 237, 313, 544, 562, 571, 625
BcoDI GTCTC 2 cut(s) 242, 254
BfaI CTAG 2 cut(s) 335, 675
BfoI RGCGCY 1 cut(s) 380
BfuI GTATCC 1 cut(s) 92
BglII AGATCT 1 cut(s) 132
BisI GCNGC 9 cut(s) 41, 80, 109, 219, 433, 558, 672, 804, 816
BlsI GCNGC 9 cut(s) 42, 81, 110, 220, 434, 559, 673, 805, 817
Bme18I GGWCC 6 cut(s) 49, 138, 169, 385, 621, 727
BmeT110I CYCGRG 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 719
BmgT120I GGNCC 9 cut(s) 49, 138, 169, 238, 385, 451, 540, 621, 727
BmiI GGNNCC 8 cut(s) 119, 149, 195, 310, 541, 609, 622, 729
BmsI GCATC 1 cut(s) 537
BoxI GACNNNNGTC 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 58
BpmI CTGGAG 3 cut(s) 104, 187, 209
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 9 cut(s) 53, 152, 236, 237, 313, 544, 562, 571, 625
Bsa29I ATCGAT 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 2 cut(s) 242, 254
BsaJI CCNNGG 3 cut(s) 80, 235, 617
BsaWI WCCGGW 2 cut(s) 140, 171
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 330, 536, 561, 617, 623, 736, 776
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 121, 781
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 1 cut(s) 618
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 80, 235, 617
BseGI GGATG 3 cut(s) 466, 552, 815
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 8 cut(s) 190, 330, 536, 561, 617, 623, 736, 776
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 2 cut(s) 121, 781
BseRI GAGGAG 3 cut(s) 29, 32, 35
BseXI GCAGC 3 cut(s) 27, 658, 815
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 5 cut(s) 82, 246, 280, 344, 773
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 240, 453, 542
BshNI GGYRCC 1 cut(s) 308
BshTI ACCGGT 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 4 cut(s) 36, 89, 213, 414
BsiHKCI CYCGRG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 8 cut(s) 190, 330, 536, 561, 617, 623, 736, 776
BsmAI GTCTC 2 cut(s) 242, 254
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 3 cut(s) 240, 453, 542
Bso31I GGTCTC 2 cut(s) 242, 254
BsoBI CYCGRG 1 cut(s) 235
Bsp1286I GDGCHC 4 cut(s) 36, 89, 213, 414
Bsp143I GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
BspANI GGCC 3 cut(s) 240, 453, 542
BspCNI CTCAG 1 cut(s) 266
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 5 cut(s) 82, 246, 280, 344, 773
BspLI GGNNCC 8 cut(s) 119, 149, 195, 310, 541, 609, 622, 729
BspPI GGATC 6 cut(s) 175, 188, 201, 730, 747, 787
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 242, 254
BsrBI CCGCTC 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 2 cut(s) 121, 781
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 3 cut(s) 80, 235, 617
BssMI GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
BssSI CACGAG 3 cut(s) 44, 389, 413
Bst2BI CACGAG 3 cut(s) 44, 389, 413
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 400
BstC8I GCNNGC 4 cut(s) 209, 223, 242, 321
BstDEI CTNAG 1 cut(s) 253
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 466, 552, 815
BstFNI CGCG 5 cut(s) 82, 246, 280, 344, 773
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 3 cut(s) 246, 282, 379
BstKTI GATC 7 cut(s) 135, 183, 196, 300, 725, 742, 782
BstMAI GTCTC 2 cut(s) 242, 254
BstMBI GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 6 cut(s) 40, 93, 432, 441, 450, 812
BstNI CCWGG 1 cut(s) 618
BstPAI GACNNNNGTC 1 cut(s) 254
BstUI CGCG 5 cut(s) 82, 246, 280, 344, 773
BstV1I GCAGC 3 cut(s) 27, 658, 815
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 4 cut(s) 132, 180, 193, 739
BstYI RGATCY 4 cut(s) 132, 180, 193, 739
Bsu15I ATCGAT 1 cut(s) 459
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 3 cut(s) 240, 453, 542
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 80
BtrI CACGTC 1 cut(s) 719
BtsCI GGATG 3 cut(s) 466, 552, 815
Cac8I GCNNGC 4 cut(s) 209, 223, 242, 321
CfoI GCGC 3 cut(s) 246, 282, 379
Cfr10I RCCGGY 1 cut(s) 171
Cfr13I GGNCC 9 cut(s) 49, 138, 169, 238, 385, 451, 540, 621, 727
Cfr42I CCGCGG 1 cut(s) 83
Cfr9I CCCGGG 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 2 cut(s) 290, 397
CspAI ACCGGT 1 cut(s) 171
CviAII CATG 1 cut(s) 162
CviQI GTAC 2 cut(s) 290, 397
DdeI CTNAG 1 cut(s) 253
DpnI GATC 7 cut(s) 134, 182, 195, 299, 724, 741, 781
DpnII GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 3 cut(s) 111, 544, 600
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 2 cut(s) 242, 254
Eco47I GGWCC 6 cut(s) 49, 138, 169, 385, 621, 727
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 1 cut(s) 165
FaiI YATR 2 cut(s) 163, 483
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 1 cut(s) 586
FatI CATG 1 cut(s) 161
FauI CCCGC 3 cut(s) 84, 478, 778
Fnu4HI GCNGC 9 cut(s) 41, 80, 109, 219, 433, 558, 672, 804, 816
FokI GGATG 2 cut(s) 453, 559
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 9 cut(s) 41, 80, 109, 219, 433, 558, 672, 804, 816
FspBI CTAG 2 cut(s) 335, 675
GlaI GCGC 3 cut(s) 245, 281, 378
GluI GCNGC 9 cut(s) 41, 80, 109, 219, 433, 558, 672, 804, 816
GsuI CTGGAG 3 cut(s) 104, 187, 209
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 3 cut(s) 240, 453, 542
HhaI GCGC 3 cut(s) 246, 282, 379
Hin1II CATG 1 cut(s) 165
Hin6I GCGC 3 cut(s) 244, 280, 377
HinP1I GCGC 3 cut(s) 244, 280, 377
HinfI GANTC 3 cut(s) 272, 678, 760
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 6 cut(s) 127, 198, 385, 727, 739, 824
Hpy188III TCNNGA 2 cut(s) 259, 295
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 4 cut(s) 14, 280, 449, 535
HpyAV CCTTC 4 cut(s) 334, 412, 488, 574
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 3 cut(s) 372, 399, 718
HpyCH4V TGCA 2 cut(s) 165, 803
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 93, 432, 441, 450, 812
HpyF3I CTNAG 1 cut(s) 253
HpySE526I ACGT 3 cut(s) 372, 399, 718
Hsp92II CATG 1 cut(s) 165
HspAI GCGC 3 cut(s) 244, 280, 377
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
LmnI GCTCC 6 cut(s) 84, 123, 153, 346, 409, 613
Lsp1109I GCAGC 3 cut(s) 27, 658, 815
LweI GCATC 1 cut(s) 537
MaeI CTAG 2 cut(s) 335, 675
MaeII ACGT 3 cut(s) 372, 399, 718
MalI GATC 7 cut(s) 134, 182, 195, 299, 724, 741, 781
MbiI CCGCTC 1 cut(s) 349
MboI GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
MboII GAAGA 3 cut(s) 58, 415, 755
MflI RGATCY 4 cut(s) 132, 180, 193, 739
MhlI GDGCHC 4 cut(s) 36, 89, 213, 414
MlyI GAGTC 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 618
MvnI CGCG 5 cut(s) 82, 246, 280, 344, 773
MwoI GCNNNNNNNGC 6 cut(s) 40, 93, 432, 441, 450, 812
NciI CCSGG 9 cut(s) 53, 152, 236, 237, 313, 544, 562, 571, 625
NdeII GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 8 cut(s) 119, 149, 195, 310, 541, 609, 622, 729
PcsI WCGNNNNNNNCGW 3 cut(s) 360, 369, 396
PfeI GAWTC 2 cut(s) 678, 760
PfoI TCCNGGA 3 cut(s) 51, 569, 623
PinAI ACCGGT 1 cut(s) 171
PkrI GCNGC 9 cut(s) 42, 81, 110, 220, 434, 559, 673, 805, 817
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Ppu21I YACGTR 1 cut(s) 400
PshAI GACNNNNGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 8 cut(s) 119, 149, 195, 310, 541, 609, 622, 729
PspPI GGNCC 9 cut(s) 49, 138, 169, 238, 385, 451, 540, 621, 727
PsuI RGATCY 4 cut(s) 132, 180, 193, 739
RsaI GTAC 2 cut(s) 291, 398
RsaNI GTAC 2 cut(s) 290, 397
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 83
SatI GCNGC 9 cut(s) 41, 80, 109, 219, 433, 558, 672, 804, 816
Sau3AI GATC 7 cut(s) 132, 180, 193, 297, 722, 739, 779
Sau96I GGNCC 9 cut(s) 49, 138, 169, 238, 385, 451, 540, 621, 727
SchI GAGTC 1 cut(s) 266
SduI GDGCHC 4 cut(s) 36, 89, 213, 414
SfaNI GCATC 1 cut(s) 537
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 6 cut(s) 49, 138, 169, 385, 621, 727
SmaI CCCGGG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SspMI CTAG 2 cut(s) 335, 675
SstI GAGCTC 1 cut(s) 213
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 3 cut(s) 375, 402, 721
TaqI TCGA 8 cut(s) 9, 30, 258, 270, 300, 363, 459, 788
TauI GCSGC 6 cut(s) 82, 111, 221, 435, 560, 818
TfiI GAWTC 2 cut(s) 678, 760
TseI GCWGC 3 cut(s) 40, 671, 803
TspDTI ATGAA 2 cut(s) 316, 455
TspMI CCCGGG 1 cut(s) 235
VpaK11BI GGWCC 6 cut(s) 49, 138, 169, 385, 621, 727
XmaI CCCGGG 1 cut(s) 235
XspI CTAG 2 cut(s) 335, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.