MD05G1194700.v1.1

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003015
Physical Location & Seq
Reverse (-)
32218355 .. 32220427
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1194700.v1.1.491

Sequence Viewer

Length: 834 bp
ATGTCGTTCGAGGAGGAGGAGGAGGAGGCATTCGAGCACACGCTTCTCGTGGTCCGCGAGGTCGCGGTCTTCAAAATTCCGCCGCGGAGCACTTCCGGCGGGTACAAGTGCGGCGAGTGGCTCCAGTCCGATAAGATCTGGTCGGGTCGGCTCCGGGTCGTGTCGTGCAAGGACCGGTGCGAGATCCGGCTGGAGGATCCGAACTCCAGCGAGCTGTTCGCCGCCTGCTTCGTCCACCCGGGCCAGCGCGAGACCTCCGTCGAGACCGTGCTCGACTCGTCGCGCTACTTCGTGCTCAAGATCGAGGACGGCACCGGGAAGCACGCCTTCATCGGACTAGGATTCGCGGAGCGGAACGAGGCTTTCGATTTCAACGTGGCGCTTTCGGACCACGAGAAGTACGTGAAGAGGGAGCACGAGAAGGAGAGCAACGGCGGCGAAACCAGCGACGACTGCCACATCGATATTCATCCCGCCGTCAATCACAGATTGAAAGAAGGTGAGACGATAAGAATAAATGTGAAGCCGAAGCCGAAGCCGACGAGTGGGGTCGGGATGTTGTCCTCAGCCGGGCTATCCGGGGCGGCCAAGGCGAAACCGATTGGTTTGGGGTTGGCTCCGCCACCGGGGTCCGGGAAAGTTAGGCCGGCTCTGCCACCACCTCCCAATGACCCTGTTGCTGCTAGAATCAGCTCTGGTAGTGTTAAGTCGCCGGATAACACAAGACGTGGATCCGACCCGTTATCGGATCTTTCCCAGACTGAGAGGAATCTTCCTCCAGCGACCGGATCAGGGTCGGCAAAGACAAGTGCAGCAGGATGGGCAGCCTTCTGA

Protein Analysis

278

Amino Acids

29.89

Weight (kDa)

6.1

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 11 - 175 4.3e-58 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 352
AccII CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
AclWI GGATC 7 cut(s) 178, 191, 204, 726, 739, 756, 796
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 1 cut(s) 75
AfaI GTAC 2 cut(s) 104, 401
AfiI CCNNNNNNNGG 8 cut(s) 193, 545, 570, 626, 632, 745, 785, 792
AgeI ACCGGT 1 cut(s) 174
AgsI TTSAA 3 cut(s) 73, 373, 493
AjiI CACGTC 1 cut(s) 728
AluBI AGCT 2 cut(s) 214, 693
AluI AGCT 2 cut(s) 214, 693
Alw21I GWGCWC 5 cut(s) 39, 92, 273, 297, 417
Alw26I GTCTC 3 cut(s) 245, 257, 497
AlwI GGATC 7 cut(s) 178, 191, 204, 726, 739, 756, 796
Ama87I CYCGRG 1 cut(s) 238
AoxI GGCC 3 cut(s) 241, 585, 644
ApeKI GCWGC 3 cut(s) 680, 812, 824
ApoI RAATTY 1 cut(s) 75
AsiGI ACCGGT 1 cut(s) 174
AspLEI GCGC 3 cut(s) 249, 285, 382
AspS9I GGNCC 5 cut(s) 52, 172, 241, 388, 630
AsuC2I CCSGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
AsuHPI GGTGA 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 4 cut(s) 52, 172, 388, 630
BamHI GGATCC 2 cut(s) 196, 731
BanI GGYRCC 1 cut(s) 311
BauI CACGAG 3 cut(s) 47, 392, 416
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 5 cut(s) 39, 92, 273, 297, 417
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 2 cut(s) 667, 824
BccI CCATC 1 cut(s) 813
BceAI ACGGC 3 cut(s) 325, 448, 461
BcnI CCSGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
BcoDI GTCTC 3 cut(s) 245, 257, 497
BfaI CTAG 2 cut(s) 338, 684
BfoI RGCGCY 1 cut(s) 383
BglII AGATCT 1 cut(s) 135
BisI GCNGC 8 cut(s) 83, 112, 222, 436, 585, 681, 813, 825
BlsI GCNGC 8 cut(s) 84, 113, 223, 437, 586, 682, 814, 826
Bme1390I CCNGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
Bme18I GGWCC 4 cut(s) 52, 172, 388, 630
BmeT110I CYCGRG 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 728
BmgT120I GGNCC 5 cut(s) 52, 172, 241, 388, 630
BmiI GGNNCC 7 cut(s) 122, 152, 198, 313, 618, 631, 733
BmrFI CCNGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
BoxI GACNNNNGTC 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 4 cut(s) 107, 190, 212, 762
Bpu10I CCTNAGC 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 2 cut(s) 245, 257
BsaJI CCNNGG 5 cut(s) 83, 238, 579, 588, 626
BsaWI WCCGGW 2 cut(s) 174, 785
Bsc4I CCNNNNNNNGG 8 cut(s) 193, 545, 570, 626, 632, 745, 785, 792
Bse118I RCCGGY 2 cut(s) 174, 646
Bse1I ACTGG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 468
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 5 cut(s) 83, 238, 579, 588, 626
BseGI GGATG 3 cut(s) 469, 561, 824
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 8 cut(s) 193, 545, 570, 626, 632, 745, 785, 792
BseMII CTCAG 2 cut(s) 579, 753
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 5 cut(s) 26, 29, 32, 35, 38
BseXI GCAGC 2 cut(s) 667, 824
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 3 cut(s) 243, 587, 646
BshNI GGYRCC 1 cut(s) 311
BshTI ACCGGT 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 786
BsiHKAI GWGCWC 5 cut(s) 39, 92, 273, 297, 417
BsiHKCI CYCGRG 1 cut(s) 238
BslI CCNNNNNNNGG 8 cut(s) 193, 545, 570, 626, 632, 745, 785, 792
BsmAI GTCTC 3 cut(s) 245, 257, 497
BsmBI CGTCTC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 3 cut(s) 243, 587, 646
Bso31I GGTCTC 2 cut(s) 245, 257
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 5 cut(s) 39, 92, 273, 297, 417
Bsp143I GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
BspANI GGCC 3 cut(s) 243, 587, 646
BspCNI CTCAG 2 cut(s) 578, 754
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
BspLI GGNNCC 7 cut(s) 122, 152, 198, 313, 618, 631, 733
BspPI GGATC 7 cut(s) 178, 191, 204, 726, 739, 756, 796
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 245, 257
BsrBI CCGCTC 1 cut(s) 352
BsrFI RCCGGY 2 cut(s) 174, 646
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 2 cut(s) 174, 646
BssECI CCNNGG 5 cut(s) 83, 238, 579, 588, 626
BssMI GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
BssSI CACGAG 3 cut(s) 47, 392, 416
BssT1I CCWWGG 1 cut(s) 588
Bst2BI CACGAG 3 cut(s) 47, 392, 416
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 5 cut(s) 212, 226, 245, 324, 648
BstDEI CTNAG 2 cut(s) 565, 762
BstDSI CCRYGG 1 cut(s) 83
BstF5I GGATG 3 cut(s) 469, 561, 824
BstFNI CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 3 cut(s) 249, 285, 382
BstKTI GATC 7 cut(s) 138, 186, 199, 303, 734, 751, 791
BstMAI GTCTC 3 cut(s) 245, 257, 497
BstMBI GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 7 cut(s) 96, 435, 444, 453, 590, 652, 821
BstPAI GACNNNNGTC 1 cut(s) 257
BstSCI CCNGG 8 cut(s) 153, 237, 238, 314, 569, 578, 625, 632
BstUI CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
BstV1I GCAGC 2 cut(s) 667, 824
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 5 cut(s) 135, 183, 196, 731, 748
BstYI RGATCY 5 cut(s) 135, 183, 196, 731, 748
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 3 cut(s) 243, 587, 646
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 83
BtrI CACGTC 1 cut(s) 728
BtsCI GGATG 3 cut(s) 469, 561, 824
Cac8I GCNNGC 5 cut(s) 212, 226, 245, 324, 648
CfoI GCGC 3 cut(s) 249, 285, 382
Cfr10I RCCGGY 2 cut(s) 174, 646
Cfr13I GGNCC 5 cut(s) 52, 172, 241, 388, 630
Cfr42I CCGCGG 1 cut(s) 86
Cfr9I CCCGGG 1 cut(s) 238
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 2 cut(s) 103, 400
CspAI ACCGGT 1 cut(s) 174
CviQI GTAC 2 cut(s) 103, 400
DdeI CTNAG 2 cut(s) 565, 762
DpnI GATC 7 cut(s) 137, 185, 198, 302, 733, 750, 790
DpnII GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
EciI GGCGGA 2 cut(s) 69, 609
Eco130I CCWWGG 1 cut(s) 588
Eco31I GGTCTC 2 cut(s) 245, 257
Eco47I GGWCC 4 cut(s) 52, 172, 388, 630
Eco88I CYCGRG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 497
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FauI CCCGC 2 cut(s) 92, 481
Fnu4HI GCNGC 8 cut(s) 83, 112, 222, 436, 585, 681, 813, 825
FokI GGATG 2 cut(s) 456, 568
Fsp4HI GCNGC 8 cut(s) 83, 112, 222, 436, 585, 681, 813, 825
FspBI CTAG 2 cut(s) 338, 684
GlaI GCGC 3 cut(s) 248, 284, 381
GluI GCNGC 8 cut(s) 83, 112, 222, 436, 585, 681, 813, 825
GsuI CTGGAG 4 cut(s) 107, 190, 212, 762
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 3 cut(s) 243, 587, 646
HhaI GCGC 3 cut(s) 249, 285, 382
Hin6I GCGC 3 cut(s) 247, 283, 380
HinP1I GCGC 3 cut(s) 247, 283, 380
HinfI GANTC 4 cut(s) 275, 342, 687, 769
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 7 cut(s) 130, 201, 335, 388, 736, 748, 833
Hpy188III TCNNGA 3 cut(s) 262, 298, 553
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 4 cut(s) 263, 283, 452, 544
HpyAV CCTTC 3 cut(s) 337, 415, 491
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 3 cut(s) 375, 402, 727
HpyCH4V TGCA 2 cut(s) 168, 812
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 435, 444, 453, 590, 652, 821
HpyF3I CTNAG 2 cut(s) 565, 762
HpySE526I ACGT 3 cut(s) 375, 402, 727
HspAI GCGC 3 cut(s) 247, 283, 380
KroI GCCGGC 1 cut(s) 646
KroNI GCCGGC 1 cut(s) 648
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
LmnI GCTCC 6 cut(s) 87, 126, 156, 349, 412, 622
Lsp1109I GCAGC 2 cut(s) 667, 824
MaeI CTAG 2 cut(s) 338, 684
MaeII ACGT 3 cut(s) 375, 402, 727
MalI GATC 7 cut(s) 137, 185, 198, 302, 733, 750, 790
MbiI CCGCTC 1 cut(s) 352
MboI GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
MboII GAAGA 3 cut(s) 61, 418, 764
MflI RGATCY 5 cut(s) 135, 183, 196, 731, 748
MhlI GDGCHC 5 cut(s) 39, 92, 273, 297, 417
MluCI AATT 1 cut(s) 75
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 759
MroNI GCCGGC 1 cut(s) 646
MseI TTAA 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 85
MspR9I CCNGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
Mva1269I GAATGC 1 cut(s) 29
MvnI CGCG 6 cut(s) 57, 65, 85, 249, 283, 347
MwoI GCNNNNNNNGC 7 cut(s) 96, 435, 444, 453, 590, 652, 821
NaeI GCCGGC 1 cut(s) 648
NciI CCSGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
NdeII GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
NgoMIV GCCGGC 1 cut(s) 646
NlaIV GGNNCC 7 cut(s) 122, 152, 198, 313, 618, 631, 733
PcsI WCGNNNNNNNCGW 4 cut(s) 54, 363, 372, 399
PctI GAATGC 1 cut(s) 29
PdiI GCCGGC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 342, 687, 769
PfoI TCCNGGA 1 cut(s) 632
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 8 cut(s) 84, 113, 223, 437, 586, 682, 814, 826
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Ppu21I YACGTR 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 257
PspN4I GGNNCC 7 cut(s) 122, 152, 198, 313, 618, 631, 733
PspPI GGNCC 5 cut(s) 52, 172, 241, 388, 630
PsuI RGATCY 5 cut(s) 135, 183, 196, 731, 748
RsaI GTAC 2 cut(s) 104, 401
RsaNI GTAC 2 cut(s) 103, 400
SacII CCGCGG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 8 cut(s) 83, 112, 222, 436, 585, 681, 813, 825
Sau3AI GATC 7 cut(s) 135, 183, 196, 300, 731, 748, 788
Sau96I GGNCC 5 cut(s) 52, 172, 241, 388, 630
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 8 cut(s) 155, 239, 240, 316, 571, 580, 627, 634
SduI GDGCHC 5 cut(s) 39, 92, 273, 297, 417
SetI ASST 9 cut(s) 63, 216, 257, 378, 405, 502, 664, 695, 730
Sfr303I CCGCGG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 86
SinI GGWCC 4 cut(s) 52, 172, 388, 630
SmaI CCCGGG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 1 cut(s) 75
SspMI CTAG 2 cut(s) 338, 684
StyD4I CCNGG 8 cut(s) 153, 237, 238, 314, 569, 578, 625, 632
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 3 cut(s) 378, 405, 730
TaqI TCGA 7 cut(s) 9, 33, 261, 273, 303, 366, 462
TasI AATT 1 cut(s) 75
TauI GCSGC 5 cut(s) 85, 114, 224, 438, 587
TfiI GAWTC 3 cut(s) 342, 687, 769
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TseI GCWGC 3 cut(s) 680, 812, 824
TspDTI ATGAA 2 cut(s) 319, 458
TspGWI ACGGA 1 cut(s) 247
TspMI CCCGGG 1 cut(s) 238
VpaK11BI GGWCC 4 cut(s) 52, 172, 388, 630
XapI RAATTY 1 cut(s) 75
XmaI CCCGGG 1 cut(s) 238
XspI CTAG 2 cut(s) 338, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.