AT1G50060

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002121
Physical Location & Seq
Forward (+)
18551121 .. 18552660
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50060.1

Sequence Viewer

Length: 486 bp
ATGAATACTTTCAAAACCCCTTTTCTCGTCATCGTTGCGATTAGCTTTCTTGTTGTTGCAACCAATGCTCAAAACACTCCACAAGACTACCTTAACTCTCACAACACCGCTCGTGCGCAGGTTGGAGTACCGAACGTGGTGTGGGACACAACTCTCGCGGCCTACGCATTGAACTATTCAAATTTTAGAAAAGCCGACTGCAACCTTGTTCACTCCAACGGACCATATGGAGAAAACCTAGCCAAGGGAAGTAGTTCCTCGTTCTCGGCCATCTCGGCCGTCAAACTTTGGGTCGACGAGAAACCTTACTACAGCTACGCATACAATAATTGCACAGGTGGAAAACAATGCTTGCATTACACGCAAGTGGTGTGGAGAGATTCAGTGAAGATAGGGTGTGCTAGGGTTCAATGCACCAATACTTGGTGGTTCGTGAGTTGTAACTATAACTCACCCGGTAATTGGGTCGGAGAGTACCCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

161

Amino Acids

18.01

Weight (kDa)

8.5

Isoelectric Point (pI)

20.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 31 - 149 2.3e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 109
AccB7I CCANNNNNTGG 1 cut(s) 423
AccBSI CCGCTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 158
AciI CCGC 2 cut(s) 108, 158
AcoI YGGCCR 2 cut(s) 267, 276
AcsI RAATTY 1 cut(s) 181
AfaI GTAC 2 cut(s) 129, 476
AfiI CCNNNNNNNGG 3 cut(s) 244, 423, 462
AgsI TTSAA 4 cut(s) 13, 172, 180, 410
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 2 cut(s) 45, 315
AluI AGCT 2 cut(s) 45, 315
AoxI GGCC 3 cut(s) 159, 267, 276
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 2 cut(s) 8, 253
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 221
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 1 cut(s) 444
AvaII GGWCC 1 cut(s) 221
BauI CACGAG 1 cut(s) 111
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 263
BcnI CCSGG 1 cut(s) 456
BfaI CTAG 2 cut(s) 239, 402
BfmI CTRYAG 1 cut(s) 310
BfuAI ACCTGC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 456
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 456
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 423, 462
BseDI CCNNGG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 244, 423, 462
BseX3I CGGCCG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 3 cut(s) 161, 269, 278
BsiEI CGRYCG 1 cut(s) 279
BsiSI CCGG 1 cut(s) 456
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 3 cut(s) 244, 423, 462
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 3 cut(s) 161, 269, 278
BspACI CCGC 2 cut(s) 108, 158
BspANI GGCC 3 cut(s) 161, 269, 278
BspFNI CGCG 1 cut(s) 158
BspMI ACCTGC 1 cut(s) 109
BsrBI CCGCTC 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 243
BssSI CACGAG 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 353
BstENI CCTNNNNNAGG 1 cut(s) 242
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 118
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 4 cut(s) 65, 164, 275, 361
BstSCI CCNGG 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 1 cut(s) 158
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 3 cut(s) 161, 269, 278
BtsIMutI CAGTG 1 cut(s) 390
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 128, 475
CspCI CAANNNNNGTGG 2 cut(s) 353, 388
CviJI RGCY 7 cut(s) 45, 161, 194, 242, 269, 278, 315
CviKI_1 RGCY 7 cut(s) 45, 161, 194, 242, 269, 278, 315
CviQI GTAC 2 cut(s) 128, 475
EaeI YGGCCR 2 cut(s) 267, 276
EagI CGGCCG 1 cut(s) 276
EclXI CGGCCG 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaiI YATR 4 cut(s) 226, 228, 322, 447
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 158
FauNDI CATATG 1 cut(s) 226
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 2 cut(s) 239, 402
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 1 cut(s) 117
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 3 cut(s) 161, 269, 278
HapII CCGG 1 cut(s) 456
HhaI GCGC 1 cut(s) 118
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 380
HpaII CCGG 1 cut(s) 456
HphI GGTGA 1 cut(s) 444
Hpy166II GTNNAC 2 cut(s) 211, 295
Hpy188I TCNGA 1 cut(s) 470
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 2 cut(s) 211, 295
Hpy99I CGWCG 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 5 cut(s) 59, 201, 333, 355, 414
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 164, 275, 361
HpySE526I ACGT 1 cut(s) 135
HspAI GCGC 1 cut(s) 116
LpnPI CCDG 3 cut(s) 104, 321, 469
MaeI CTAG 2 cut(s) 239, 402
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 440
MbiI CCGCTC 1 cut(s) 110
MboII GAAGA 1 cut(s) 400
MluCI AATT 3 cut(s) 181, 328, 460
MmeI TCCRAC 3 cut(s) 103, 240, 448
MnlI CCTC 1 cut(s) 268
MroXI GAANNNNTTC 2 cut(s) 8, 253
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 365
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 456
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 4 cut(s) 65, 164, 275, 361
NciI CCSGG 1 cut(s) 456
NdeI CATATG 1 cut(s) 226
NmeAIII GCCGAG 2 cut(s) 245, 254
NsbI TGCGCA 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 365
PdmI GAANNNNTTC 2 cut(s) 8, 253
PfeI GAWTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 221
RsaI GTAC 2 cut(s) 129, 476
RsaNI GTAC 2 cut(s) 128, 475
RseI CAYNNNNRTG 1 cut(s) 365
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 1 cut(s) 159
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 456
SetI ASST 9 cut(s) 47, 93, 123, 138, 207, 240, 307, 317, 340
SfcI CTRYAG 1 cut(s) 310
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 365
Sse9I AATT 3 cut(s) 181, 328, 460
SsiI CCGC 2 cut(s) 108, 158
SspMI CTAG 2 cut(s) 239, 402
StyD4I CCNGG 1 cut(s) 454
StyI CCWWGG 1 cut(s) 243
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 294
TasI AATT 3 cut(s) 181, 328, 460
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 390
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 390
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 221
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 2 cut(s) 8, 253
XspI CTAG 2 cut(s) 239, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.