FvH4_2g02940

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002121
Physical Location & Seq
Forward (+)
2285803 .. 2286225
423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02940.t1

Sequence Viewer

Length: 423 bp
ATGCCCAGAACTTTTAACAAGACTACCTCAATGCACACAATGCATGCAGCTCGCGCACAAGTTGGAGTGGCAAACATCAAATGGGACAGCATTGTTGCTGCCTATGCTCTAAACTACACCAAGTCGAGGATTGCTGATTGCAATCTCGTCCTCTCAGAAGGGCGTTACGGCAAGAATCTAGCCAAAGGCAGTGGCTCGTTCACGGCCACTGCCGCGGTGAATTTGTGGGTGGCGGAAAAGCCTTACTACAACTACACGTCTGATGCTTGCACCGGCGGAAAGCAGTGCCTGCACTATACTCAAGTGGTGTGGGCATACTCTGTTAGGTTGGGGTGTGCTAGGCTTCAATGTAGCAATTTGTGGTGGTTTGTTACATGCAGCTATGATCCACCAAGCAACTACATCGGGGAGCATCCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

141

Amino Acids

15.64

Weight (kDa)

9.05

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 16 - 128 5.5e-12 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 54, 215
AciI CCGC 4 cut(s) 213, 215, 233, 276
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 220
AfiI CCNNNNNNNGG 1 cut(s) 126
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 1 cut(s) 347
AjiI CACGTC 1 cut(s) 258
AluBI AGCT 2 cut(s) 50, 381
AluI AGCT 2 cut(s) 50, 381
AlwI GGATC 1 cut(s) 380
AlwNI CAGNNNCTG 1 cut(s) 289
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 3 cut(s) 47, 98, 378
ApoI RAATTY 1 cut(s) 220
AspLEI GCGC 1 cut(s) 56
AsuHPI GGTGA 1 cut(s) 229
BbvI GCAGC 3 cut(s) 59, 85, 390
BceAI ACGGC 2 cut(s) 184, 219
BcgI CGANNNNNNTGC 4 cut(s) 32, 66, 385, 419
BfaI CTAG 2 cut(s) 179, 339
BisI GCNGC 4 cut(s) 48, 99, 213, 379
BlsI GCNGC 4 cut(s) 49, 100, 214, 380
BmgBI CACGTC 1 cut(s) 258
BmsI GCATC 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 213
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse118I RCCGGY 1 cut(s) 272
Bse8I GATNNNNATC 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 412
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 59, 85, 390
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 2 cut(s) 54, 215
BshFI GGCC 1 cut(s) 206
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 1 cut(s) 206
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 4 cut(s) 213, 215, 233, 276
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 167
BspFNI CGCG 2 cut(s) 54, 215
BspPI GGATC 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 1 cut(s) 385
BstAPI GCANNNNNTGC 2 cut(s) 40, 289
BstC8I GCNNGC 4 cut(s) 45, 52, 268, 290
BstDEI CTNAG 1 cut(s) 154
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 2 cut(s) 54, 215
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 5 cut(s) 40, 53, 104, 212, 289
BstNSI RCATGY 2 cut(s) 47, 378
BstUI CGCG 2 cut(s) 54, 215
BstV1I GCAGC 3 cut(s) 59, 85, 390
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 213
BtrI CACGTC 1 cut(s) 258
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 3 cut(s) 196, 207, 290
BtsIMutI CAGTG 3 cut(s) 196, 207, 290
Cac8I GCNNGC 4 cut(s) 45, 52, 268, 290
CaiI CAGNNNCTG 1 cut(s) 289
CfoI GCGC 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 272
Cfr42I CCGCGG 1 cut(s) 216
CspCI CAANNNNNGTGG 4 cut(s) 172, 207, 290, 325
CviAII CATG 2 cut(s) 44, 375
CviJI RGCY 7 cut(s) 50, 182, 195, 206, 241, 343, 381
CviKI_1 RGCY 7 cut(s) 50, 182, 195, 206, 241, 343, 381
DdeI CTNAG 1 cut(s) 154
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 204
EciI GGCGGA 2 cut(s) 248, 291
EcoT22I ATGCAT 1 cut(s) 45
FaeI CATG 2 cut(s) 47, 378
FaiI YATR 6 cut(s) 45, 105, 297, 316, 376, 384
FaqI GGGAC 1 cut(s) 98
FatI CATG 2 cut(s) 43, 374
Fnu4HI GCNGC 4 cut(s) 48, 99, 213, 379
FokI GGATG 1 cut(s) 399
Fsp4HI GCNGC 4 cut(s) 48, 99, 213, 379
FspBI CTAG 2 cut(s) 179, 339
GlaI GCGC 1 cut(s) 55
GluI GCNGC 4 cut(s) 48, 99, 213, 379
HaeIII GGCC 1 cut(s) 206
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 56
Hin1II CATG 2 cut(s) 47, 378
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HinfI GANTC 1 cut(s) 175
HpaII CCGG 1 cut(s) 273
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 2 cut(s) 157, 262
Hpy8I GTNNAC 1 cut(s) 201
HpyAV CCTTC 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 7 cut(s) 34, 43, 47, 141, 270, 292, 378
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 53, 104, 212, 289
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 47, 378
HspAI GCGC 1 cut(s) 54
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 1 cut(s) 409
LpnPI CCDG 3 cut(s) 19, 286, 302
Lsp1109I GCAGC 3 cut(s) 59, 85, 390
LweI GCATC 1 cut(s) 253
MaeI CTAG 2 cut(s) 179, 339
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 164, 370
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MluCI AATT 2 cut(s) 220, 355
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 3 cut(s) 37, 120, 161
Mph1103I ATGCAT 1 cut(s) 45
MseI TTAA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 273
MvnI CGCG 2 cut(s) 54, 215
MwoI GCNNNNNNNGC 5 cut(s) 40, 53, 104, 212, 289
NdeII GATC 1 cut(s) 385
NlaIII CATG 2 cut(s) 47, 378
NsiI ATGCAT 1 cut(s) 45
NspI RCATGY 2 cut(s) 47, 378
PaeI GCATGC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 4 cut(s) 49, 100, 214, 380
PstNI CAGNNNCTG 1 cut(s) 289
SacII CCGCGG 1 cut(s) 216
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 4 cut(s) 48, 99, 213, 379
Sau3AI GATC 1 cut(s) 385
SetI ASST 5 cut(s) 29, 52, 260, 329, 383
SfaNI GCATC 1 cut(s) 253
Sfr303I CCGCGG 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 272
SgrBI CCGCGG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SphI GCATGC 1 cut(s) 47
Sse9I AATT 2 cut(s) 220, 355
SsiI CCGC 4 cut(s) 213, 215, 233, 276
SspMI CTAG 2 cut(s) 179, 339
TaiI ACGT 1 cut(s) 260
TaqI TCGA 1 cut(s) 125
TasI AATT 2 cut(s) 220, 355
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 3 cut(s) 196, 214, 290
TseI GCWGC 3 cut(s) 47, 98, 378
TspRI CASTG 3 cut(s) 196, 214, 290
XapI RAATTY 1 cut(s) 220
XceI RCATGY 2 cut(s) 47, 378
XspI CTAG 2 cut(s) 179, 339
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.