Rorug05G0528200

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002121
Physical Location & Seq
Reverse (-)
71744805 .. 71746568
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0528200.1

Sequence Viewer

Length: 1764 bp
ATGATGCGCAGCAGCAGCTCAAGAGGTATGCTTTCTTCTCTCTCCGTCAACAATGTCATCATGGCTTTGTTTCATTCGAGACAGAGACAAACCCAAACCCATGTAGAGAGGCTTGTGAGAAAACCACCCTCAAAGCCCAAAGTTGAGGAGGATGCCTTGAAGTTGTTCCGTGAAATGCTTCACAGCCGTCCTCTGCCTTGTGTTGTTCGTTTCACTCAGTTATTGGGTCAACTTGTCAAATTGAAACACTATTCTGCCGTCATCTCTTTGAATAGACAGATGGTTCTCTGTGGAATCGCTTCTGATGACTATACTCTAAACATTATCATTAAGTGCTACTGCCATTTGAATCAAATGGGGTTTGGCTTATCTGTCTTGGGAAAATTCTTCAAATTGGGTCTTCAACCAGACGTCGTCACCTTCACCACTCTAATCAACGGCTTTGTTCTCCACAATCAAGTGCCTCAGGCTGCACGAATTTTCACCAAAATGCTGGAGGCAGGAGGTCATTGTGAGCCAAACGCGGTTACGTTCAACACACTAATAAAGGGCTTTTGCATGAGGGGAGACAACACTGCTGCGATTCAATTACTTGGGAAGATGGAAGAAAGAGGATGCGAGCCTAACATTGTTTCCTTTAGCACCATCATTGGCAGTCTTTGCAAGCACACACTAATTGATGAAGCATTCAACCTCTTCTCAGAAATGATCAGTAGAGGTATTACTCCAGACGTTGTTACTTACACGTCTTTGATTCACGGAGTTTGCAAACTAGGCCAGTGGAAACAAGCTACGAGGTTGCTTGATGAAATGTTGAGTAAAGGTATCTTTCCAGATGTTTGCACCTTCAATGTCTTGGTTGATACTTTCTGTAAGGAAGGGATGGTCATGGAAGCCAAAAGTGTGATTCAAAAGATGATTCAAAGACATATTCAGCCTGATACGATTACATACAACTCACTTATGGACGGTTACTGTTTGCGAGGAGAAATGGACAAGGCAAGACAAGTTTTTGATGTTATGATTAGCAAGGGCTCCATGGTTGATGTTCGGAGCTGTAGCATATTGATACATGGATATTGCAAGGGTAAAAAGGTAGATCAGGCTTACAAGATTTTCAAGGAAATGGCTCGTATGGAACTTGTTCCTGATACCGTTACTTATACCACTCTTATTGATGGTTTTTGCAAAGTGGGGAGAATTCAAGAAGCAGAAGAGTTGTTCTCTGAGATGCAGGGTTGTGGCCAGCTTCCAAATGTTCAGACTTATGCTGTGATACTTGATGGCCTGTGTAACAATCAGCAACTTTCTACGGCTGTAGAATTGCTCACAGAGATGGAAGCCAGAGAACTAGAACTCGATATTGTAATTTACAATATTGTTATTGAAGGTTTCTGCAAAGCTGGAGAAATTGAATCCGCAAGAGACCTCTTCTGTGGTTTGTCATCAAAAGGAGTTCAGCATAATGTCAGAACAAACACTGTAATGATTCATGGACTTTGTCATCATGGCTTCATAATTGAAGCAGAAAAGTTGCTGAGAGAAATGGGAGGGAAAGGATGTTCTCCAAATGGTTGGACCTATAACACCATTATCCGAGGTTTTATCAATAACAATGAGACATCAAGGGCTACGAGACTTATTCAAGAAATGCTTGAGAGGGGTTTCTCTGCAGATGCATCAACTATGGAATTGATTGTTGATTTATTGTCGAAGGATACAGTAGATCCTGGTTTATTAGCATTGCTTAAAGATTCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

587

Amino Acids

65.64

Weight (kDa)

7.18

Isoelectric Point (pI)

28.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 66 - 115 9.8e-06 PPR repeat family
PPR_3 PF13812 128 - 185 1.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 133 - 164 1e-06 PPR repeat
PPR_2 PF13041 136 - 186 2.7e-13 PPR repeat family
PPR_long PF17177 152 - 291 1.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 171 - 202 1.2e-10 PPR repeat
PPR_3 PF13812 172 - 219 1.4e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 173 - 222 3.7e-16 PPR repeat family
TPR_24 PF23276 175 - 268 3e-06 Fungal tetratrico peptide repeats
PPR PF01535 176 - 206 2.3e-06 PPR repeat
PPR_1 PF12854 204 - 236 1.4e-11 PPR repeat
PPR_2 PF13041 208 - 257 5.5e-18 PPR repeat family
PPR PF01535 211 - 241 1.9e-06 PPR repeat
PPR_long PF17177 224 - 342 9e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 232 - 291 2.3e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 239 - 271 2.4e-12 PPR repeat
PPR_2 PF13041 243 - 292 8.6e-18 PPR repeat family
PPR PF01535 246 - 276 1.2e-07 PPR repeat
MRP-S27 PF10037 247 - 309 7.2e-07 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 274 - 306 3.7e-10 PPR repeat
TPR_24 PF23276 275 - 376 1.2e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 278 - 326 1.9e-14 PPR repeat family
PPR_1 PF12854 310 - 341 1.3e-11 PPR repeat
PPR_2 PF13041 313 - 362 2.6e-14 PPR repeat family
PPR PF01535 316 - 345 3.1e-08 PPR repeat
PPR_1 PF12854 345 - 376 5.3e-07 PPR repeat
PPR_2 PF13041 349 - 397 2e-16 PPR repeat family
PPR PF01535 352 - 379 8.9e-06 PPR repeat
PPR_long PF17177 365 - 469 1.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 371 - 428 1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 380 - 412 4.3e-15 PPR repeat
PPR_2 PF13041 383 - 432 2.1e-18 PPR repeat family
TPR_24 PF23276 385 - 478 4.4e-09 Fungal tetratrico peptide repeats
PPR PF01535 386 - 415 3.8e-10 PPR repeat
PPR_1 PF12854 415 - 447 2.9e-06 PPR repeat
PPR_1 PF12854 450 - 478 7.2e-08 PPR repeat
PPR_2 PF13041 454 - 502 2.8e-13 PPR repeat family
PPR_2 PF13041 494 - 536 2.5e-10 PPR repeat family
PPR PF01535 494 - 521 6.4e-06 PPR repeat
PPR_1 PF12854 519 - 551 3.4e-06 PPR repeat
PPR_2 PF13041 527 - 570 4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
Acc16I TGCGCA 1 cut(s) 8
AccII CGCG 1 cut(s) 524
AciI CCGC 2 cut(s) 524, 1419
AclWI GGATC 1 cut(s) 1721
AcoI YGGCCR 1 cut(s) 1243
AcsI RAATTY 3 cut(s) 383, 477, 1200
AcyI GRCGYC 1 cut(s) 411
AflIII ACRYGT 1 cut(s) 744
AjiI CACGTC 1 cut(s) 747
AjnI CCWGG 1 cut(s) 1729
AloI GAACNNNNNNTCC 2 cut(s) 1546, 1578
AluBI AGCT 5 cut(s) 18, 791, 1056, 1249, 1403
AluI AGCT 5 cut(s) 18, 791, 1056, 1249, 1403
Alw26I GTCTC 6 cut(s) 73, 79, 561, 1419, 1613, 1630
AlwI GGATC 1 cut(s) 1721
AoxI GGCC 3 cut(s) 775, 1243, 1285
ApeKI GCWGC 5 cut(s) 9, 12, 15, 470, 578
ApoI RAATTY 3 cut(s) 383, 477, 1200
Asp700I GAANNNNTTC 4 cut(s) 164, 177, 298, 1143
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 1578
AsuHPI GGTGA 3 cut(s) 409, 415, 475
AvaII GGWCC 1 cut(s) 1578
AxyI CCTNAGG 1 cut(s) 465
BalI TGGCCA 1 cut(s) 1245
BanII GRGCYC 1 cut(s) 1037
BarI GAAGNNNNNNTAC 2 cut(s) 19, 51
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 5 cut(s) 21, 24, 27, 457, 565
BccI CCATC 7 cut(s) 274, 595, 653, 877, 1172, 1277, 1330
BceAI ACGGC 4 cut(s) 171, 242, 454, 1329
BciT130I CCWGG 1 cut(s) 1731
BciVI GTATCC 1 cut(s) 1711
BclI TGATCA 1 cut(s) 708
BcoDI GTCTC 6 cut(s) 73, 79, 561, 1419, 1613, 1630
BfaI CTAG 2 cut(s) 773, 1352
BfmI CTRYAG 3 cut(s) 1057, 1317, 1671
BfuI GTATCC 1 cut(s) 1711
BisI GCNGC 5 cut(s) 10, 13, 16, 471, 579
BlsI GCNGC 5 cut(s) 11, 14, 17, 472, 580
Bme1390I CCNGG 1 cut(s) 1731
Bme18I GGWCC 1 cut(s) 1578
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 1 cut(s) 1578
BmiI GGNNCC 1 cut(s) 1036
BmrFI CCNGG 1 cut(s) 1731
BmsI GCATC 5 cut(s) 142, 605, 1221, 1666, 1688
BpiI GAAGAC 1 cut(s) 392
BplI GAGNNNNNCTC 2 cut(s) 1208, 1240
BpmI CTGGAG 3 cut(s) 515, 711, 1425
BpuEI CTTGAG 2 cut(s) 4, 1676
BsaHI GRCGYC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 1419
BsaJI CCNNGG 2 cut(s) 1038, 1597
Bse1I ACTGG 1 cut(s) 778
Bse21I CCTNAGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 1742
BseBI CCWGG 1 cut(s) 1731
BseDI CCNNGG 2 cut(s) 1038, 1597
BseGI GGATG 4 cut(s) 157, 620, 888, 1565
BseMI GCAATG 1 cut(s) 1742
BseMII CTCAG 5 cut(s) 230, 479, 714, 1218, 1529
BseNI ACTGG 1 cut(s) 778
BseRI GAGGAG 2 cut(s) 161, 999
BseXI GCAGC 5 cut(s) 21, 24, 27, 457, 565
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 3 cut(s) 777, 1245, 1287
BsmAI GTCTC 6 cut(s) 73, 79, 561, 1419, 1613, 1630
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 3 cut(s) 777, 1245, 1287
Bso31I GGTCTC 1 cut(s) 1419
Bsp1286I GDGCHC 1 cut(s) 1037
Bsp143I GATC 3 cut(s) 708, 1099, 1726
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 2 cut(s) 524, 1419
BspANI GGCC 3 cut(s) 777, 1245, 1287
BspCNI CTCAG 5 cut(s) 229, 478, 713, 1219, 1530
BspFNI CGCG 1 cut(s) 524
BspLI GGNNCC 1 cut(s) 1036
BspMAI CTGCAG 1 cut(s) 1675
BspPI GGATC 1 cut(s) 1721
BspTNI GGTCTC 1 cut(s) 1419
BsrDI GCAATG 1 cut(s) 1742
BsrI ACTGG 1 cut(s) 778
BssECI CCNNGG 2 cut(s) 1038, 1597
BssMI GATC 3 cut(s) 708, 1099, 1726
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 1038
Bst2UI CCWGG 1 cut(s) 1731
Bst4CI ACNGT 5 cut(s) 971, 977, 1156, 1483, 1723
Bst6I CTCTTC 3 cut(s) 701, 1209, 1436
BstACI GRCGYC 1 cut(s) 411
BstAPI GCANNNNNTGC 1 cut(s) 660
BstC8I GCNNGC 3 cut(s) 620, 665, 1247
BstDEI CTNAG 5 cut(s) 216, 465, 700, 1227, 1538
BstDSI CCRYGG 1 cut(s) 1038
BstF5I GGATG 4 cut(s) 157, 620, 888, 1565
BstFNI CGCG 1 cut(s) 524
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 3 cut(s) 711, 1102, 1729
BstMAI GTCTC 6 cut(s) 73, 79, 561, 1419, 1613, 1630
BstMBI GATC 3 cut(s) 708, 1099, 1726
BstMWI GCNNNNNNNGC 3 cut(s) 15, 660, 774
BstNI CCWGG 1 cut(s) 1731
BstSCI CCNGG 1 cut(s) 1729
BstSFI CTRYAG 3 cut(s) 1057, 1317, 1671
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 5 cut(s) 21, 24, 27, 457, 565
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 1726
BstXI CCANNNNNNTGG 2 cut(s) 493, 1575
BstYI RGATCY 1 cut(s) 1726
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 1 cut(s) 1711
BsuRI GGCC 3 cut(s) 777, 1245, 1287
BtgI CCRYGG 1 cut(s) 1038
BtrI CACGTC 1 cut(s) 747
BtsCI GGATG 4 cut(s) 157, 620, 888, 1565
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 3 cut(s) 573, 785, 1479
Cac8I GCNNGC 3 cut(s) 620, 665, 1247
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 1578
CspCI CAANNNNNGTGG 2 cut(s) 1156, 1191
CviAII CATG 8 cut(s) 61, 101, 559, 889, 1039, 1073, 1493, 1508
DdeI CTNAG 5 cut(s) 216, 465, 700, 1227, 1538
DpnI GATC 3 cut(s) 710, 1101, 1728
DpnII GATC 3 cut(s) 708, 1099, 1726
EaeI YGGCCR 1 cut(s) 1243
Eam1104I CTCTTC 3 cut(s) 701, 1209, 1436
EarI CTCTTC 3 cut(s) 701, 1209, 1436
Eco130I CCWWGG 1 cut(s) 1038
Eco24I GRGCYC 1 cut(s) 1037
Eco31I GGTCTC 1 cut(s) 1419
Eco47I GGWCC 1 cut(s) 1578
Eco81I CCTNAGG 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 1200
EcoRII CCWGG 1 cut(s) 1729
EcoT14I CCWWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 1681
EcoT38I GRGCYC 1 cut(s) 1037
ErhI CCWWGG 1 cut(s) 1038
FaeI CATG 8 cut(s) 64, 104, 562, 892, 1042, 1076, 1496, 1511
FalI AAGNNNNNCTT 2 cut(s) 1638, 1670
FatI CATG 8 cut(s) 60, 100, 558, 888, 1038, 1072, 1492, 1507
FbaI TGATCA 1 cut(s) 708
Fnu4HI GCNGC 5 cut(s) 10, 13, 16, 471, 579
FokI GGATG 4 cut(s) 164, 627, 895, 1572
FriOI GRGCYC 1 cut(s) 1037
Fsp4HI GCNGC 5 cut(s) 10, 13, 16, 471, 579
FspBI CTAG 2 cut(s) 773, 1352
FspI TGCGCA 1 cut(s) 8
GlaI GCGC 1 cut(s) 8
GluI GCNGC 5 cut(s) 10, 13, 16, 471, 579
GsuI CTGGAG 3 cut(s) 515, 711, 1425
HaeIII GGCC 3 cut(s) 777, 1245, 1287
HhaI GCGC 1 cut(s) 9
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 8 cut(s) 64, 104, 562, 892, 1042, 1076, 1496, 1511
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HincII GTYRAC 2 cut(s) 49, 230
HindII GTYRAC 2 cut(s) 49, 230
HinfI GANTC 9 cut(s) 294, 349, 583, 754, 907, 919, 1415, 1489, 1754
HphI GGTGA 3 cut(s) 409, 415, 475
Hpy166II GTNNAC 2 cut(s) 49, 230
Hpy188I TCNGA 8 cut(s) 304, 703, 1053, 1228, 1263, 1472, 1598, 1763
Hpy188III TCNNGA 7 cut(s) 21, 78, 728, 833, 1148, 1205, 1646
Hpy8I GTNNAC 2 cut(s) 49, 230
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 5 cut(s) 430, 856, 872, 1382, 1708
HpyCH4III ACNGT 5 cut(s) 971, 977, 1156, 1483, 1723
HpyCH4IV ACGT 4 cut(s) 411, 530, 732, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 660, 774
HpyF3I CTNAG 5 cut(s) 216, 465, 700, 1227, 1538
HpySE526I ACGT 4 cut(s) 411, 530, 732, 746
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 8 cut(s) 64, 104, 562, 892, 1042, 1076, 1496, 1511
HspAI GCGC 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 3 cut(s) 708, 1099, 1726
LmnI GCTCC 2 cut(s) 1040, 1053
Lsp1109I GCAGC 5 cut(s) 21, 24, 27, 457, 565
LweI GCATC 5 cut(s) 142, 605, 1221, 1666, 1688
MaeI CTAG 2 cut(s) 773, 1352
MaeII ACGT 4 cut(s) 411, 530, 732, 746
MaeIII GTNAC 6 cut(s) 415, 526, 736, 971, 1156, 1292
MalI GATC 3 cut(s) 710, 1101, 1728
MboI GATC 3 cut(s) 708, 1099, 1726
MboII GAAGA 8 cut(s) 27, 379, 392, 610, 617, 688, 1226, 1423
MflI RGATCY 1 cut(s) 1726
MhlI GDGCHC 1 cut(s) 1037
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MmeI TCCRAC 1 cut(s) 1556
Mox20I TGGCCA 1 cut(s) 1245
Mph1103I ATGCAT 1 cut(s) 1681
MroXI GAANNNNTTC 4 cut(s) 164, 177, 298, 1143
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 2 cut(s) 330, 1749
MslI CAYNNNNRTG 3 cut(s) 488, 1334, 1484
Msp20I TGGCCA 1 cut(s) 1245
MspR9I CCNGG 1 cut(s) 1731
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 1 cut(s) 1731
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 15, 660, 774
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 3 cut(s) 708, 1099, 1726
NlaIII CATG 8 cut(s) 64, 104, 562, 892, 1042, 1076, 1496, 1511
NlaIV GGNNCC 1 cut(s) 1036
NmuCI GTSAC 1 cut(s) 415
NsbI TGCGCA 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 1681
PctI GAATGC 1 cut(s) 686
PdmI GAANNNNTTC 4 cut(s) 164, 177, 298, 1143
PfeI GAWTC 9 cut(s) 294, 349, 583, 754, 907, 919, 1415, 1489, 1754
PflFI GACNNNGTC 2 cut(s) 413, 1500
PkrI GCNGC 5 cut(s) 11, 14, 17, 472, 580
Psp6I CCWGG 1 cut(s) 1729
PspGI CCWGG 1 cut(s) 1729
PspN4I GGNNCC 1 cut(s) 1036
PspPI GGNCC 1 cut(s) 1578
PstI CTGCAG 1 cut(s) 1675
PsuI RGATCY 1 cut(s) 1726
PsyI GACNNNGTC 2 cut(s) 413, 1500
RseI CAYNNNNRTG 3 cut(s) 488, 1334, 1484
SaqAI TTAA 2 cut(s) 330, 1749
SatI GCNGC 5 cut(s) 10, 13, 16, 471, 579
Sau3AI GATC 3 cut(s) 708, 1099, 1726
Sau96I GGNCC 1 cut(s) 1578
ScrFI CCNGG 1 cut(s) 1731
SduI GDGCHC 1 cut(s) 1037
SfaNI GCATC 5 cut(s) 142, 605, 1221, 1666, 1688
SfcI CTRYAG 3 cut(s) 1057, 1317, 1671
SinI GGWCC 1 cut(s) 1578
SmiMI CAYNNNNRTG 3 cut(s) 488, 1334, 1484
SmlI CTYRAG 2 cut(s) 19, 1655
SmoI CTYRAG 2 cut(s) 19, 1655
SsiI CCGC 2 cut(s) 524, 1419
SspI AATATT 1 cut(s) 1378
SspMI CTAG 2 cut(s) 773, 1352
StyD4I CCNGG 1 cut(s) 1729
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 5 cut(s) 971, 977, 1156, 1483, 1723
TaiI ACGT 4 cut(s) 414, 533, 735, 749
TaqI TCGA 3 cut(s) 77, 1359, 1712
TfiI GAWTC 9 cut(s) 294, 349, 583, 754, 907, 919, 1415, 1489, 1754
Tru1I TTAA 2 cut(s) 330, 1749
Tru9I TTAA 2 cut(s) 330, 1749
TscAI CASTG 3 cut(s) 580, 785, 1486
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 5 cut(s) 9, 12, 15, 470, 578
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 5 cut(s) 62, 696, 822, 1481, 1504
TspGWI ACGGA 3 cut(s) 34, 158, 774
TspRI CASTG 3 cut(s) 580, 785, 1486
Tth111I GACNNNGTC 2 cut(s) 413, 1500
VpaK11BI GGWCC 1 cut(s) 1578
XapI RAATTY 3 cut(s) 383, 477, 1200
XmnI GAANNNNTTC 4 cut(s) 164, 177, 298, 1143
XspI CTAG 2 cut(s) 773, 1352
ZraI GACGTC 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.