MD10G1112900.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002121
Physical Location & Seq
Reverse (-)
18636712 .. 18637200
489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1112900.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGCGTTGTGGGTTGCAGTTCTTTGTATCGTGGTCATGGCACTAAGTCAAGTTCATCCCTCTCATGCTCAAAACTCACAGCAAGACTTCCTCAACGCACACAACGCGGCGCGAGCGCAAGTCAACGTGCCGGATATCGTGTGGGACAACGCCGTAGCGGCGTATGCTCAAAACTACGCCAACTCGAGAATTGGTGACTGCAATTTGGTGCATTCGAATTCCAATGGGACTTACGGCGAGAACTTGGCTAAAGGGAGTGGCTCGTTCACGGGCGTGGCGGCTGTGAACTTGTGGGTGGCGGAGAAGCCTAACTACGATTACAACTCTAATTCTTGTGTTGGTGGGCAGCAATGCTTGCATTACACGCAAGTGATTTGGAAGAACTCGGTTAAGTTGGGGTGTGCTAGGGTTCAGTGTACCAATGGGTGGTGGTTTGTGACTTGCAACTATTCCCCACCTGGAAACTACGTTGGGCAACGTCCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

163

Amino Acids

17.72

Weight (kDa)

7.6

Isoelectric Point (pI)

28.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 31 - 150 1.4e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 426
AccII CGCG 2 cut(s) 107, 112
AciI CCGC 4 cut(s) 107, 158, 278, 299
AcsI RAATTY 1 cut(s) 217
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 1 cut(s) 426
AjnI CCWGG 1 cut(s) 457
AleI CACNNNNGTG 2 cut(s) 272, 368
Ama87I CYCGRG 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 217
AspLEI GCGC 2 cut(s) 112, 118
AsuHPI GGTGA 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 184
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbvI GCAGC 1 cut(s) 358
BceAI ACGGC 2 cut(s) 137, 250
BciT130I CCWGG 1 cut(s) 459
BfaI CTAG 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 4 cut(s) 108, 159, 279, 347
BlsI GCNGC 4 cut(s) 109, 160, 280, 348
Bme1390I CCNGG 1 cut(s) 459
BmeT110I CYCGRG 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 426
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 426
BseMI GCAATG 1 cut(s) 356
BseXI GCAGC 1 cut(s) 358
Bsh1236I CGCG 2 cut(s) 107, 112
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 2 cut(s) 158, 241
BslI CCNNNNNNNGG 1 cut(s) 426
BsmFI GGGAC 2 cut(s) 158, 241
BsmI GAATGC 1 cut(s) 211
BsoBI CYCGRG 1 cut(s) 184
Bsp119I TTCGAA 1 cut(s) 215
BspACI CCGC 4 cut(s) 107, 158, 278, 299
BspFNI CGCG 2 cut(s) 107, 112
BspT104I TTCGAA 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 2 cut(s) 114, 356
BstDEI CTNAG 1 cut(s) 44
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 2 cut(s) 107, 112
BstHHI GCGC 2 cut(s) 112, 118
BstMWI GCNNNNNNNGC 6 cut(s) 104, 113, 158, 164, 355, 364
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 457
BstUI CGCG 2 cut(s) 107, 112
BstV1I GCAGC 1 cut(s) 358
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 2 cut(s) 114, 356
CfoI GCGC 2 cut(s) 112, 118
Csp6I GTAC 1 cut(s) 417
CviAII CATG 2 cut(s) 37, 65
CviJI RGCY 4 cut(s) 248, 261, 281, 307
CviKI_1 RGCY 4 cut(s) 248, 261, 281, 307
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 1 cut(s) 44
EciI GGCGGA 1 cut(s) 314
Eco32I GATATC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 136
FaeI CATG 2 cut(s) 40, 68
FaiI YATR 3 cut(s) 38, 66, 165
FaqI GGGAC 2 cut(s) 158, 241
FatI CATG 2 cut(s) 36, 64
Fnu4HI GCNGC 4 cut(s) 108, 159, 279, 347
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 108, 159, 279, 347
FspBI CTAG 1 cut(s) 405
GlaI GCGC 2 cut(s) 111, 117
GluI GCNGC 4 cut(s) 108, 159, 279, 347
HapII CCGG 1 cut(s) 131
HhaI GCGC 2 cut(s) 112, 118
Hin1II CATG 2 cut(s) 40, 68
Hin6I GCGC 2 cut(s) 110, 116
HinP1I GCGC 2 cut(s) 110, 116
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 4 cut(s) 124, 267, 286, 417
Hpy188III TCNNGA 1 cut(s) 186
Hpy8I GTNNAC 4 cut(s) 124, 267, 286, 417
HpyCH4IV ACGT 3 cut(s) 126, 468, 478
HpyCH4V TGCA 5 cut(s) 17, 201, 211, 358, 444
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 113, 158, 164, 355, 364
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 3 cut(s) 126, 468, 478
Hsp92II CATG 2 cut(s) 40, 68
HspAI GCGC 2 cut(s) 110, 116
LpnPI CCDG 3 cut(s) 144, 444, 471
Lsp1109I GCAGC 1 cut(s) 358
MaeI CTAG 1 cut(s) 405
MaeII ACGT 3 cut(s) 126, 468, 478
MaeIII GTNAC 2 cut(s) 194, 436
MboII GAAGA 1 cut(s) 391
MluCI AATT 4 cut(s) 189, 202, 217, 328
MnlI CCTC 2 cut(s) 70, 101
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 2 cut(s) 272, 368
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 2 cut(s) 107, 112
MwoI GCNNNNNNNGC 6 cut(s) 104, 113, 158, 164, 355, 364
NlaIII CATG 2 cut(s) 40, 68
NmuCI GTSAC 2 cut(s) 194, 436
NspV TTCGAA 1 cut(s) 215
OliI CACNNNNGTG 2 cut(s) 272, 368
PaeR7I CTCGAG 1 cut(s) 184
PctI GAATGC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 4 cut(s) 109, 160, 280, 348
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 2 cut(s) 272, 368
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 4 cut(s) 108, 159, 279, 347
ScrFI CCNGG 1 cut(s) 459
SetI ASST 4 cut(s) 129, 460, 471, 481
Sfr274I CTCGAG 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 272, 368
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 4 cut(s) 189, 202, 217, 328
SsiI CCGC 4 cut(s) 107, 158, 278, 299
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 457
TaiI ACGT 3 cut(s) 129, 471, 481
TaqI TCGA 2 cut(s) 185, 215
TasI AATT 4 cut(s) 189, 202, 217, 328
TauI GCSGC 3 cut(s) 110, 161, 281
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 2 cut(s) 194, 436
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 2 cut(s) 194, 436
TspDTI ATGAA 1 cut(s) 44
TspRI CASTG 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 426
XapI RAATTY 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 184
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.