FvH4_2g02921

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002121
Physical Location & Seq
Forward (+)
2282673 .. 2283455
783 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02921.t1

Sequence Viewer

Length: 495 bp
ATGGCTTCCCTTCTTACCATTCTTCTCTGCTTCATAGTCTTGACAGTGGTGCTGCTGGTTCAACCCTCCCATGCGCAAAACTCACCACAAGACTACGTCGATGCTCACAACGCCGCTCGTTTACGAGTTGGTGTACCAAATATTACGTGGGACGACACTGTTGCGGCCTATGCTCAACACTACGCCAATTTAAGAAGCGGCGACTGCAGTCTCGTGCATTCGCATGGGCCTTACGGTGAGAACTTGGCCTACGGCTATGGCAGCGGCGCAATCACAGGCGTCAACGCCGTGAACTTGTGGCTGGAAGAGAAGCCTTACTACAACCACAACTCCAATTGTTGTGTTGGTGGAGAATGTCTTCACTATACGCAAGTGATTTGGCATTACTCTATCAGGCTGGGGTGTGCCAGGGTCCAATGCAGCAACCCTGGGTGTTGGTTTGTCACCTGCAACTATGATCCACCGGGCAACTACTATGGCAAGCGTCCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

165

Amino Acids

18.19

Weight (kDa)

6.41

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 33 - 152 1.8e-16 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 455
Acc16I TGCGCA 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 116
AciI CCGC 4 cut(s) 114, 164, 198, 264
AclWI GGATC 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 135
AgsI TTSAA 1 cut(s) 62
AjnI CCWGG 2 cut(s) 407, 427
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 1 cut(s) 452
AoxI GGCC 3 cut(s) 165, 227, 246
ApeKI GCWGC 3 cut(s) 52, 261, 420
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 2 cut(s) 76, 269
AspS9I GGNCC 2 cut(s) 227, 412
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 3 cut(s) 75, 248, 436
AvaII GGWCC 1 cut(s) 412
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
BauI CACGAG 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 350
BbvI GCAGC 3 cut(s) 39, 273, 432
BceAI ACGGC 2 cut(s) 268, 272
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 409, 429
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 7 cut(s) 53, 114, 165, 199, 262, 265, 421
BlsI GCNGC 7 cut(s) 54, 115, 166, 200, 263, 266, 422
Bme1390I CCNGG 3 cut(s) 409, 429, 465
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 227, 412
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 3 cut(s) 409, 429, 465
BmsI GCATC 1 cut(s) 91
BoxI GACNNNNGTC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 465
BsaAI YACGTR 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 408, 427, 428
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
BseBI CCWGG 2 cut(s) 409, 429
BseDI CCNNGG 3 cut(s) 408, 427, 428
BseXI GCAGC 3 cut(s) 39, 273, 432
BseYI CCCAGC 1 cut(s) 397
BshFI GGCC 3 cut(s) 167, 229, 248
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 167, 229, 248
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 4 cut(s) 114, 164, 198, 264
BspANI GGCC 3 cut(s) 167, 229, 248
BspLI GGNNCC 1 cut(s) 413
BspMAI CTGCAG 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 1 cut(s) 452
BsrBI CCGCTC 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 408, 427, 428
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 2 cut(s) 409, 429
Bst4CI ACNGT 3 cut(s) 46, 160, 236
Bst6I CTCTTC 1 cut(s) 300
BstACI GRCGYC 1 cut(s) 279
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 482
BstHHI GCGC 2 cut(s) 76, 269
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 4 cut(s) 110, 170, 204, 261
BstNI CCWGG 2 cut(s) 409, 429
BstPAI GACNNNNGTC 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 407, 427, 463
BstSFI CTRYAG 1 cut(s) 205
BstV1I GCAGC 3 cut(s) 39, 273, 432
BstV2I GAAGAC 1 cut(s) 350
BsuRI GGCC 3 cut(s) 167, 229, 248
BtsIMutI CAGTG 2 cut(s) 51, 156
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 482
CfoI GCGC 2 cut(s) 76, 269
Cfr13I GGNCC 2 cut(s) 227, 412
CseI GACGC 2 cut(s) 268, 473
Csp6I GTAC 1 cut(s) 134
CviAII CATG 2 cut(s) 71, 224
CviJI RGCY 8 cut(s) 5, 167, 229, 248, 255, 301, 313, 397
CviKI_1 RGCY 8 cut(s) 5, 167, 229, 248, 255, 301, 313, 397
CviQI GTAC 1 cut(s) 134
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 407, 427
FaeI CATG 2 cut(s) 74, 227
FaiI YATR 8 cut(s) 35, 72, 171, 225, 258, 366, 456, 477
FaqI GGGAC 1 cut(s) 164
FatI CATG 2 cut(s) 70, 223
Fnu4HI GCNGC 7 cut(s) 53, 114, 165, 199, 262, 265, 421
Fsp4HI GCNGC 7 cut(s) 53, 114, 165, 199, 262, 265, 421
FspI TGCGCA 1 cut(s) 75
GlaI GCGC 2 cut(s) 75, 268
GluI GCNGC 7 cut(s) 53, 114, 165, 199, 262, 265, 421
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 3 cut(s) 167, 229, 248
HapII CCGG 1 cut(s) 464
HgaI GACGC 2 cut(s) 268, 473
HhaI GCGC 2 cut(s) 76, 269
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 2 cut(s) 74, 227
Hin6I GCGC 2 cut(s) 74, 267
HinP1I GCGC 2 cut(s) 74, 267
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HpaII CCGG 1 cut(s) 464
HphI GGTGA 3 cut(s) 75, 248, 436
Hpy166II GTNNAC 4 cut(s) 122, 134, 283, 292
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 4 cut(s) 122, 134, 283, 292
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 3 cut(s) 46, 160, 236
HpyCH4IV ACGT 2 cut(s) 96, 146
HpyCH4V TGCA 4 cut(s) 207, 217, 420, 450
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 170, 204, 261
HpySE526I ACGT 2 cut(s) 96, 146
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 2 cut(s) 74, 227
HspAI GCGC 2 cut(s) 74, 267
Kzo9I GATC 1 cut(s) 457
Lsp1109I GCAGC 3 cut(s) 39, 273, 432
LweI GCATC 1 cut(s) 91
MaeII ACGT 2 cut(s) 96, 146
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 459
MbiI CCGCTC 1 cut(s) 116
MboI GATC 1 cut(s) 457
MboII GAAGA 3 cut(s) 14, 317, 350
MfeI CAATTG 1 cut(s) 334
MluCI AATT 2 cut(s) 187, 334
MnlI CCTC 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 1 cut(s) 191
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 3 cut(s) 409, 429, 465
MunI CAATTG 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 2 cut(s) 409, 429
MwoI GCNNNNNNNGC 4 cut(s) 110, 170, 204, 261
NciI CCSGG 1 cut(s) 465
NdeII GATC 1 cut(s) 457
NlaIII CATG 2 cut(s) 74, 227
NlaIV GGNNCC 1 cut(s) 413
NmuCI GTSAC 1 cut(s) 442
NsbI TGCGCA 1 cut(s) 75
PaqCI CACCTGC 1 cut(s) 455
PasI CCCWGGG 1 cut(s) 428
PctI GAATGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 357
PflFI GACNNNGTC 1 cut(s) 95
PkrI GCNGC 7 cut(s) 54, 115, 166, 200, 263, 266, 422
Ppu21I YACGTR 1 cut(s) 147
PshAI GACNNNNGTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 407, 427
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 2 cut(s) 407, 427
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 2 cut(s) 227, 412
PsrI GAACNNNNNNTAC 2 cut(s) 233, 265
PstI CTGCAG 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 7 cut(s) 53, 114, 165, 199, 262, 265, 421
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 2 cut(s) 227, 412
ScrFI CCNGG 3 cut(s) 409, 429, 465
SetI ASST 3 cut(s) 99, 149, 449
SfaNI GCATC 1 cut(s) 91
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 2 cut(s) 187, 334
SsiI CCGC 4 cut(s) 114, 164, 198, 264
SspI AATATT 1 cut(s) 142
StyD4I CCNGG 3 cut(s) 407, 427, 463
TaaI ACNGT 3 cut(s) 46, 160, 236
TaiI ACGT 2 cut(s) 99, 149
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 187, 334
TauI GCSGC 4 cut(s) 116, 167, 201, 267
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 2 cut(s) 51, 163
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 52, 261, 420
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 22
TspRI CASTG 2 cut(s) 51, 163
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.