AT1G67430

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001814
Physical Location & Seq
Forward (+)
25262134 .. 25263857
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67430.1

Sequence Viewer

Length: 528 bp
ATGGTGAAGTACTCGCAAGAACCCGACAATCAGACCAAGTCTTGCAAGGCTAGAGGATCCGATCTTAGGGTTCACTTCAAGAATACTAGGGAAACAGCGCACGCAATCAGGAAGCTACCATTGATCAAGGCGAAGAGGTACCTTGAGGATGTGATAGCTCACAAGCAAGCAATTCCCTTCACACGTTTCTGCAGAGGTGTTGGGAGAACTGCTCAAGCTAAGAACAGGCACTCTAATGGTCAAGGACGTTGGCCAGCCAAGTCTGCTCAGTTTGTTCTTGATTTGCTCAAGAACGCTGAGAGCAATGCTGAGGTGAAAGGTTTGGATGTTGATGCCCTCTTCATATCACACATCCAAGTGAACCAGGCCGCAAAGCAAAGGAGAAGGACTTACCGTGCTCACGGAAGAATCAACCCTTACATGTCCAACCCATGCCACATTGAGTTGATTTTGTCAGAGAAGGAAGAGCCTGTCAAAAAAGAGCCGGAGACTCAGTTGGCTGCCAAGTCCAAGAAATCAGCAGCTTAA

Protein Analysis

175

Amino Acids

19.85

Weight (kDa)

10.12

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 17 - 152 2.6e-32 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AciI CCGC 1 cut(s) 369
AclWI GGATC 2 cut(s) 51, 64
AcoI YGGCCR 1 cut(s) 251
AfaI GTAC 2 cut(s) 11, 140
AfiI CCNNNNNNNGG 1 cut(s) 66
AflIII ACRYGT 2 cut(s) 182, 420
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 4 cut(s) 115, 158, 218, 524
AluI AGCT 4 cut(s) 115, 158, 218, 524
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 2 cut(s) 51, 64
AoxI GGCC 2 cut(s) 251, 366
ApeKI GCWGC 2 cut(s) 500, 521
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 16, 325
BalI TGGCCA 1 cut(s) 253
BamHI GGATCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 487
BciT130I CCWGG 1 cut(s) 365
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 2 cut(s) 51, 87
BfmI CTRYAG 1 cut(s) 190
BisI GCNGC 3 cut(s) 369, 501, 522
BlsI GCNGC 3 cut(s) 370, 502, 523
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 365
BmiI GGNNCC 2 cut(s) 58, 140
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 322
BplI GAGNNNNNCTC 2 cut(s) 196, 228
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 3 cut(s) 164, 198, 272
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 365
BseGI GGATG 3 cut(s) 154, 331, 351
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 4 cut(s) 281, 288, 300, 506
BseXI GCAGC 1 cut(s) 487
BshFI GGCC 2 cut(s) 253, 368
BshNI GGYRCC 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 482
BsnI GGCC 2 cut(s) 253, 368
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 56, 61, 123
BspACI CCGC 1 cut(s) 369
BspANI GGCC 2 cut(s) 253, 368
BspCNI CTCAG 4 cut(s) 280, 289, 301, 505
BspLI GGNNCC 2 cut(s) 58, 140
BspMAI CTGCAG 1 cut(s) 194
BspPI GGATC 2 cut(s) 51, 64
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 310
BssMI GATC 3 cut(s) 56, 61, 123
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 3 cut(s) 128, 344, 459
BstC8I GCNNGC 3 cut(s) 102, 168, 255
BstDEI CTNAG 6 cut(s) 65, 219, 267, 297, 309, 492
BstF5I GGATG 3 cut(s) 154, 331, 351
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 3 cut(s) 59, 64, 126
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 3 cut(s) 56, 61, 123
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 363
BstSFI CTRYAG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 2 cut(s) 253, 368
BtsCI GGATG 3 cut(s) 154, 331, 351
Cac8I GCNNGC 3 cut(s) 102, 168, 255
CfoI GCGC 1 cut(s) 100
Csp6I GTAC 2 cut(s) 10, 139
CviAII CATG 2 cut(s) 421, 432
CviQI GTAC 2 cut(s) 10, 139
DdeI CTNAG 6 cut(s) 65, 219, 267, 297, 309, 492
DpnI GATC 3 cut(s) 58, 63, 125
DpnII GATC 3 cut(s) 56, 61, 123
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 3 cut(s) 128, 344, 459
EarI CTCTTC 3 cut(s) 128, 344, 459
EcoRII CCWGG 1 cut(s) 363
FaeI CATG 2 cut(s) 424, 435
FaiI YATR 3 cut(s) 344, 422, 433
FatI CATG 2 cut(s) 420, 431
FbaI TGATCA 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 369, 501, 522
FokI GGATG 3 cut(s) 161, 338, 338
Fsp4HI GCNGC 3 cut(s) 369, 501, 522
FspBI CTAG 2 cut(s) 51, 87
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 369, 501, 522
HaeIII GGCC 2 cut(s) 253, 368
HapII CCGG 1 cut(s) 485
HhaI GCGC 1 cut(s) 100
Hin1II CATG 2 cut(s) 424, 435
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 2 cut(s) 408, 490
HpaII CCGG 1 cut(s) 485
HphI GGTGA 2 cut(s) 16, 325
Hpy166II GTNNAC 2 cut(s) 73, 361
Hpy188I TCNGA 3 cut(s) 33, 61, 457
Hpy188III TCNNGA 4 cut(s) 79, 109, 278, 289
Hpy8I GTNNAC 2 cut(s) 73, 361
HpyAV CCTTC 3 cut(s) 187, 378, 454
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 2 cut(s) 184, 247
HpyCH4V TGCA 2 cut(s) 45, 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 6 cut(s) 65, 219, 267, 297, 309, 492
HpySE526I ACGT 2 cut(s) 184, 247
Hsp92II CATG 2 cut(s) 424, 435
HspAI GCGC 1 cut(s) 98
KpnI GGTACC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 3 cut(s) 56, 61, 123
LguI GCTCTTC 1 cut(s) 459
LpnPI CCDG 7 cut(s) 94, 211, 267, 350, 377, 483, 498
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 51, 87
MaeII ACGT 2 cut(s) 184, 247
MalI GATC 3 cut(s) 58, 63, 125
MboI GATC 3 cut(s) 56, 61, 123
MboII GAAGA 4 cut(s) 145, 331, 417, 476
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 484
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 6 cut(s) 47, 129, 139, 188, 304, 347
Mox20I TGGCCA 1 cut(s) 253
MscI TGGCCA 1 cut(s) 253
MseI TTAA 1 cut(s) 526
MslI CAYNNNNRTG 2 cut(s) 234, 356
Msp20I TGGCCA 1 cut(s) 253
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 263
NdeII GATC 3 cut(s) 56, 61, 123
NlaIII CATG 2 cut(s) 424, 435
NlaIV GGNNCC 2 cut(s) 58, 140
NspI RCATGY 1 cut(s) 424
PciI ACATGT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 3 cut(s) 370, 502, 523
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PscI ACATGT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 2 cut(s) 58, 140
PstI CTGCAG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 56
PsyI GACNNNGTC 1 cut(s) 37
RsaI GTAC 2 cut(s) 11, 140
RsaNI GTAC 2 cut(s) 10, 139
RseI CAYNNNNRTG 2 cut(s) 234, 356
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 526
SatI GCNGC 3 cut(s) 369, 501, 522
Sau3AI GATC 3 cut(s) 56, 61, 123
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 234, 356
SmlI CTYRAG 3 cut(s) 143, 213, 287
SmoI CTYRAG 3 cut(s) 143, 213, 287
Sse9I AATT 1 cut(s) 171
SsiI CCGC 1 cut(s) 369
SspMI CTAG 2 cut(s) 51, 87
StyD4I CCNGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 2 cut(s) 187, 250
TasI AATT 1 cut(s) 171
TatI WGTACW 1 cut(s) 9
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 408
Tru1I TTAA 1 cut(s) 526
Tru9I TTAA 1 cut(s) 526
TseI GCWGC 2 cut(s) 500, 521
TspDTI ATGAA 1 cut(s) 331
TspGWI ACGGA 1 cut(s) 417
Tth111I GACNNNGTC 1 cut(s) 37
XceI RCATGY 1 cut(s) 424
XspI CTAG 2 cut(s) 51, 87
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.