MD17G1012400.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001814
Physical Location & Seq
Forward (+)
1099972 .. 1102214
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1012400.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGTGAAGTACTCGAGGGAGCCCAATAATCCCACCAAGTCCTGCAAGGCCCAGGGCGAGGGTCTAAGAGTTCATTTCAAGAACACGAGAGAGACTGCCCATGCCATCAGGAAGATGCATTTGGCAAAGGCCAAAAGGTACTTGGAAGATGTTTTAGCCCACAAGCAAGCCATTCCCTTCCGCCGCTTTTGCCGCGGTGTTGGTCGAACTGCGCAGGCGAAGAACCGCCAGTCCAATGGTCAGGGGCGTTGGCCGGTTAAGTCTGCTAATTTCATTCTTGATTTGCTCAAGAATGCCGAGAGCAATGCTGAGGTGAAAGGGCTTGATGTGGATTCGTTGTACGTCTCTCACATCCAGGTCAACCAGGCTCAGAAACAGAGACGCCGCACATATCGTGCTCATGGGAGAATAAATCCTTATATGTCATCTCCCTGCCATATCGAGCTGATTTTGTCAGAGAAAGAAGAGGCAGTAAAGAAAGAGCCTGATACTCAGTTGGCTCCCAGGAAGTCGAGGAAAGGTCAAGCACTGCAGAGCGGTGCTTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

184

Amino Acids

20.77

Weight (kDa)

10.34

Isoelectric Point (pI)

62.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 17 - 152 8.2e-34 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 537
AccII CGCG 1 cut(s) 195
AciI CCGC 7 cut(s) 181, 184, 193, 195, 226, 385, 537
AcoI YGGCCR 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 382
AfaI GTAC 3 cut(s) 11, 140, 341
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 4 cut(s) 51, 354, 363, 503
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 1 cut(s) 445
AluI AGCT 1 cut(s) 445
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 86, 349, 373
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 3 cut(s) 48, 129, 251
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 16, 325
AvaI CYCGRG 1 cut(s) 13
BanII GRGCYC 1 cut(s) 24
BauI CACGAG 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 309
BccI CCATC 1 cut(s) 113
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciT130I CCWGG 4 cut(s) 53, 356, 365, 505
BcoDI GTCTC 3 cut(s) 86, 349, 373
BfmI CTRYAG 1 cut(s) 530
BisI GCNGC 3 cut(s) 184, 193, 385
BlsI GCNGC 3 cut(s) 185, 194, 386
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 53, 356, 365, 505
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 2 cut(s) 21, 501
BmrFI CCNGG 4 cut(s) 53, 356, 365, 505
BmsI GCATC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 51, 52, 193, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 4 cut(s) 53, 356, 365, 505
BseDI CCNNGG 4 cut(s) 51, 52, 193, 503
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 3 cut(s) 300, 383, 506
BseNI ACTGG 1 cut(s) 229
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 3 cut(s) 50, 131, 253
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 86, 349, 373
BsmBI CGTCTC 2 cut(s) 349, 373
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 50, 131, 253
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 24, 400
BspACI CCGC 7 cut(s) 181, 184, 193, 195, 226, 385, 537
BspANI GGCC 3 cut(s) 50, 131, 253
BspCNI CTCAG 3 cut(s) 301, 382, 505
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 21, 501
BspMAI CTGCAG 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 4 cut(s) 51, 52, 193, 503
BssNI GRCGYC 1 cut(s) 382
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 4 cut(s) 53, 356, 365, 505
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 168, 216
BstDEI CTNAG 4 cut(s) 65, 309, 369, 492
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 214
BstMAI GTCTC 3 cut(s) 86, 349, 373
BstMWI GCNNNNNNNGC 2 cut(s) 189, 192
BstNI CCWGG 4 cut(s) 53, 356, 365, 505
BstSCI CCNGG 4 cut(s) 51, 354, 363, 503
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 236
BsuRI GGCC 3 cut(s) 50, 131, 253
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 351
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 2 cut(s) 168, 216
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 49
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 1 cut(s) 390
Csp6I GTAC 3 cut(s) 10, 139, 340
CviAII CATG 2 cut(s) 101, 401
CviQI GTAC 3 cut(s) 10, 139, 340
DdeI CTNAG 4 cut(s) 65, 309, 369, 492
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 13
EcoRII CCWGG 4 cut(s) 51, 354, 363, 503
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 24
Esp3I CGTCTC 2 cut(s) 349, 373
FaeI CATG 2 cut(s) 104, 404
FaiI YATR 6 cut(s) 102, 391, 402, 420, 422, 438
FatI CATG 2 cut(s) 100, 400
Fnu4HI GCNGC 3 cut(s) 184, 193, 385
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 3 cut(s) 184, 193, 385
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 1 cut(s) 213
GluI GCNGC 3 cut(s) 184, 193, 385
HaeIII GGCC 3 cut(s) 50, 131, 253
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 2 cut(s) 104, 404
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 332
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 16, 325
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 372, 457
Hpy188III TCNNGA 5 cut(s) 79, 109, 278, 289, 549
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 3 cut(s) 45, 118, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 192
HpyF3I CTNAG 4 cut(s) 65, 309, 369, 492
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 2 cut(s) 104, 404
HspAI GCGC 1 cut(s) 212
KspI CCGCGG 1 cut(s) 196
LmnI GCTCC 2 cut(s) 19, 505
LweI GCATC 1 cut(s) 105
MaeII ACGT 1 cut(s) 342
MbiI CCGCTC 1 cut(s) 537
MboII GAAGA 4 cut(s) 124, 158, 232, 476
MhlI GDGCHC 2 cut(s) 24, 400
MluCI AATT 1 cut(s) 268
MnlI CCTC 5 cut(s) 9, 52, 304, 460, 507
Mph1103I ATGCAT 1 cut(s) 120
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 4 cut(s) 53, 356, 365, 505
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 4 cut(s) 53, 356, 365, 505
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 2 cut(s) 189, 192
NlaIII CATG 2 cut(s) 104, 404
NlaIV GGNNCC 2 cut(s) 21, 501
NmeAIII GCCGAG 1 cut(s) 322
NsbI TGCGCA 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 13
PasI CCCWGGG 1 cut(s) 52
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 3 cut(s) 185, 194, 386
Psp6I CCWGG 4 cut(s) 51, 354, 363, 503
PspGI CCWGG 4 cut(s) 51, 354, 363, 503
PspN4I GGNNCC 2 cut(s) 21, 501
PspPI GGNCC 1 cut(s) 49
PspXI VCTCGAGB 1 cut(s) 13
PstI CTGCAG 1 cut(s) 534
RsaI GTAC 3 cut(s) 11, 140, 341
RsaNI GTAC 3 cut(s) 10, 139, 340
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 3 cut(s) 184, 193, 385
Sau96I GGNCC 1 cut(s) 49
ScaI AGTACT 1 cut(s) 11
ScrFI CCNGG 4 cut(s) 53, 356, 365, 505
SduI GDGCHC 2 cut(s) 24, 400
SetI ASST 6 cut(s) 140, 315, 345, 360, 447, 523
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 530
Sfr274I CTCGAG 1 cut(s) 13
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 13, 287
SmoI CTYRAG 2 cut(s) 13, 287
Sse9I AATT 1 cut(s) 268
SsiI CCGC 7 cut(s) 181, 184, 193, 195, 226, 385, 537
StyD4I CCNGG 4 cut(s) 51, 354, 363, 503
TaiI ACGT 1 cut(s) 345
TaqI TCGA 4 cut(s) 14, 205, 441, 512
TasI AATT 1 cut(s) 268
TatI WGTACW 1 cut(s) 9
TauI GCSGC 3 cut(s) 186, 195, 387
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 534
TspDTI ATGAA 2 cut(s) 62, 262
TspRI CASTG 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.