MD09G1011700.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001814
Physical Location & Seq
Forward (+)
765612 .. 767804
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1011700.v1.1.491

Sequence Viewer

Length: 624 bp
ATGTTTCTTCCATTTTCGCCCCAATTCACCTCTGACCGCCCAGACTCCTCCTTCCGCCGCCGATCAACAGCGATGGTGAAGTACTCGAGGGAGCCCGATAATCCCACCAAGTCCTGCAAGGCCCAGGGCGAGGGTCTAAGAGTTCATTTCAAGAACACGAGAGAGACTGCCCATGCCATCAGGAAGATGCATTTGGTAAAGGCCAAAAGATACTTGGAAGATGTTTTAGCCCACAAGCAAGCCATTCCCTTTCGGCGCTTTTGCCGCGGTGTTGGTCGAACTGCTCAGGCGAAGAACCGCCAGTCCAATGGTCAGGGACGTTGGCCGGTTAAGTCTGCTAATTTCATTCTTGATTTGCTCAAGAATGCTGAGAGCAATGCTGAGGTAAAAGGGCTTGATGTGGATTCGCTCTACGTCTCTCACATCCAGGTCAACCAGGCTCAGAAACAGAGACGCCGCACATATCGTGCTCATGGGAGAATAAATCCTTATATGTCATCTCCCTGCCATATCGAGTTGATTTTGTCAGAGAAAGAAGAGGCAGTAAAGAAAGAGGCCGAAACTCAGTTGGCTCCCAGGAAATCGAGGAAAGGTCAAGCACTGCAGAGCGGTGCTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

208

Amino Acids

23.6

Weight (kDa)

10.41

Isoelectric Point (pI)

69.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 41 - 176 2.5e-33 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 609
AccII CGCG 1 cut(s) 267
AciI CCGC 8 cut(s) 37, 55, 58, 265, 267, 298, 457, 609
AcoI YGGCCR 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 4 cut(s) 123, 426, 435, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 3 cut(s) 158, 421, 445
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 4 cut(s) 120, 201, 323, 555
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 2 cut(s) 19, 88
AvaI CYCGRG 1 cut(s) 85
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 472
BbvCI CCTCAGC 1 cut(s) 381
BccI CCATC 2 cut(s) 67, 185
BciT130I CCWGG 4 cut(s) 125, 428, 437, 577
BcoDI GTCTC 3 cut(s) 158, 421, 445
BfmI CTRYAG 1 cut(s) 602
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 3 cut(s) 58, 265, 457
BlsI GCNGC 3 cut(s) 59, 266, 458
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 4 cut(s) 125, 428, 437, 577
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 93, 573
BmrFI CCNGG 4 cut(s) 125, 428, 437, 577
BmsI GCATC 1 cut(s) 177
Bpu10I CCTNAGC 2 cut(s) 285, 381
BpuEI CTTGAG 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 4 cut(s) 123, 124, 265, 575
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 4 cut(s) 125, 428, 437, 577
BseDI CCNNGG 4 cut(s) 123, 124, 265, 575
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 5 cut(s) 299, 360, 372, 455, 578
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 4 cut(s) 122, 203, 325, 557
BsiHKAI GWGCWC 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 3 cut(s) 158, 421, 445
BsmBI CGTCTC 2 cut(s) 421, 445
BsmFI GGGAC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 4 cut(s) 122, 203, 325, 557
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 96, 472
Bsp143I GATC 1 cut(s) 62
BspACI CCGC 8 cut(s) 37, 55, 58, 265, 267, 298, 457, 609
BspANI GGCC 4 cut(s) 122, 203, 325, 557
BspCNI CTCAG 5 cut(s) 298, 361, 373, 454, 577
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 93, 573
BspMAI CTGCAG 1 cut(s) 606
BsrBI CCGCTC 1 cut(s) 609
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 123, 124, 265, 575
BssMI GATC 1 cut(s) 62
BssNI GRCGYC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 4 cut(s) 125, 428, 437, 577
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 6 cut(s) 137, 285, 369, 381, 441, 564
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 423
BstFNI CGCG 1 cut(s) 267
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 1 cut(s) 65
BstMAI GTCTC 3 cut(s) 158, 421, 445
BstMBI GATC 1 cut(s) 62
BstMWI GCNNNNNNNGC 1 cut(s) 264
BstNI CCWGG 4 cut(s) 125, 428, 437, 577
BstSCI CCNGG 4 cut(s) 123, 426, 435, 575
BstSFI CTRYAG 1 cut(s) 602
BstUI CGCG 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 308
BsuRI GGCC 4 cut(s) 122, 203, 325, 557
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 86
BtsCI GGATG 1 cut(s) 423
BtsI GCAGTG 1 cut(s) 599
BtsIMutI CAGTG 1 cut(s) 599
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 121
Cfr42I CCGCGG 1 cut(s) 268
CseI GACGC 1 cut(s) 462
Csp6I GTAC 1 cut(s) 82
CviAII CATG 2 cut(s) 173, 473
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 6 cut(s) 137, 285, 369, 381, 441, 564
DpnI GATC 1 cut(s) 64
DpnII GATC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 85
EcoRII CCWGG 4 cut(s) 123, 426, 435, 575
EcoT22I ATGCAT 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 96
Esp3I CGTCTC 2 cut(s) 421, 445
FaeI CATG 2 cut(s) 176, 476
FaiI YATR 6 cut(s) 174, 463, 474, 492, 494, 510
FaqI GGGAC 1 cut(s) 330
FatI CATG 2 cut(s) 172, 472
Fnu4HI GCNGC 3 cut(s) 58, 265, 457
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 3 cut(s) 58, 265, 457
GlaI GCGC 1 cut(s) 257
GluI GCNGC 3 cut(s) 58, 265, 457
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 4 cut(s) 122, 203, 325, 557
HapII CCGG 1 cut(s) 326
HgaI GACGC 1 cut(s) 462
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 2 cut(s) 176, 476
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 2 cut(s) 44, 404
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 19, 88
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 3 cut(s) 34, 444, 529
Hpy188III TCNNGA 5 cut(s) 151, 181, 350, 361, 621
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 2 cut(s) 319, 414
HpyCH4V TGCA 3 cut(s) 117, 190, 604
HpyF10VI GCNNNNNNNGC 1 cut(s) 264
HpyF3I CTNAG 6 cut(s) 137, 285, 369, 381, 441, 564
HpySE526I ACGT 2 cut(s) 319, 414
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 2 cut(s) 176, 476
HspAI GCGC 1 cut(s) 256
KspI CCGCGG 1 cut(s) 268
Kzo9I GATC 1 cut(s) 62
LmnI GCTCC 2 cut(s) 91, 577
LweI GCATC 1 cut(s) 177
MaeII ACGT 2 cut(s) 319, 414
MalI GATC 1 cut(s) 64
MbiI CCGCTC 1 cut(s) 609
MboI GATC 1 cut(s) 62
MboII GAAGA 5 cut(s) 196, 230, 304, 548, 609
MhlI GDGCHC 2 cut(s) 96, 472
MluCI AATT 2 cut(s) 23, 340
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 8 cut(s) 40, 58, 81, 124, 376, 532, 547, 579
Mph1103I ATGCAT 1 cut(s) 192
MseI TTAA 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 4 cut(s) 125, 428, 437, 577
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 4 cut(s) 125, 428, 437, 577
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 264
NdeII GATC 1 cut(s) 62
NlaIII CATG 2 cut(s) 176, 476
NlaIV GGNNCC 2 cut(s) 93, 573
NsiI ATGCAT 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 85
PasI CCCWGGG 1 cut(s) 124
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 3 cut(s) 59, 266, 458
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Psp6I CCWGG 4 cut(s) 123, 426, 435, 575
PspGI CCWGG 4 cut(s) 123, 426, 435, 575
PspN4I GGNNCC 2 cut(s) 93, 573
PspPI GGNCC 1 cut(s) 121
PspXI VCTCGAGB 1 cut(s) 85
PstI CTGCAG 1 cut(s) 606
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SacII CCGCGG 1 cut(s) 268
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 3 cut(s) 58, 265, 457
Sau3AI GATC 1 cut(s) 62
Sau96I GGNCC 1 cut(s) 121
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 4 cut(s) 125, 428, 437, 577
SduI GDGCHC 2 cut(s) 96, 472
SetI ASST 6 cut(s) 32, 322, 387, 417, 432, 595
SfaNI GCATC 1 cut(s) 177
SfcI CTRYAG 1 cut(s) 602
Sfr274I CTCGAG 1 cut(s) 85
Sfr303I CCGCGG 1 cut(s) 268
SgrBI CCGCGG 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 85
SmlI CTYRAG 2 cut(s) 85, 359
SmoI CTYRAG 2 cut(s) 85, 359
Sse9I AATT 2 cut(s) 23, 340
SsiI CCGC 8 cut(s) 37, 55, 58, 265, 267, 298, 457, 609
StyD4I CCNGG 4 cut(s) 123, 426, 435, 575
TaiI ACGT 2 cut(s) 322, 417
TaqI TCGA 4 cut(s) 86, 277, 513, 584
TasI AATT 2 cut(s) 23, 340
TatI WGTACW 1 cut(s) 81
TauI GCSGC 3 cut(s) 60, 267, 459
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 606
TspDTI ATGAA 2 cut(s) 134, 334
TspRI CASTG 1 cut(s) 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XhoI CTCGAG 1 cut(s) 85
ZrmI AGTACT 1 cut(s) 83
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.