RchiOBHm_Chr4g0430581

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001814
Physical Location & Seq
Forward (+)
55086741 .. 55089125
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39930

Sequence Viewer

Length: 552 bp
ATGGTGAAGTACTCCAAAGAGCCCGATAATCCCACCAAGTCTTGCAAAGCAAGGGGCTCTGACCTCAGAGTTCATTTCAAGAACACAAGGGAGACTGCGTTCTCAATCAGGAAGATGTCCTTGATTAAGGCAAAGCGATATCTGGAGGATGTTTTGGTTCACAAGCAAGCCATTCCATTCAGACGTTTCTGCCGTGGTGTTGGGCGTACAGCTCAGGCCAAGAACAGGCATTCCAATGGCCAGGGACGCTGGCCTGTGAAATCTGCCAAGTTCATTTTGGATTTGCTTAAGAATGCTGAGAGCAATGCTGAAGTGAAGGGTTTGGATGTTGACTCTCTTGTTGTATCTCACATCCAAGTCAACCAAGCACAGAAGCAAAGACGCCGAACATACCGTGCTCATGGAAGAATCAACCCATACATGTCATCTCCATGCCACATTGAGCTCACCTTGTCTGAAAAGGAAGAACCAGTGAAGAAAGAGCCAGACTCCCAATTGGCAACCAGCAAATCCAGGAAGTCTCAAGCTGTTCGCAGTGGTGGTTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0042592 GO:0048872 GO:0061484 GO:0065007 GO:0065008
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

183

Amino Acids

20.74

Weight (kDa)

10.36

Isoelectric Point (pI)

62.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 17 - 151 6.1e-32 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 382
AfaI GTAC 2 cut(s) 11, 208
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 2 cut(s) 240, 512
AloI GAACNNNNNNTCC 4 cut(s) 83, 115, 215, 247
AluBI AGCT 3 cut(s) 212, 445, 527
AluI AGCT 3 cut(s) 212, 445, 527
Alw21I GWGCWC 2 cut(s) 400, 447
Alw26I GTCTC 2 cut(s) 86, 525
AoxI GGCC 3 cut(s) 216, 238, 251
AsuHPI GGTGA 2 cut(s) 16, 439
BalI TGGCCA 1 cut(s) 240
BanII GRGCYC 3 cut(s) 24, 59, 447
Bbv12I GWGCWC 2 cut(s) 400, 447
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 2 cut(s) 242, 514
BcoDI GTCTC 2 cut(s) 86, 525
BfrI CTTAAG 1 cut(s) 287
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 2 cut(s) 242, 514
BmrFI CCNGG 2 cut(s) 242, 514
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 382
BsaJI CCNNGG 2 cut(s) 193, 241
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 470
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 2 cut(s) 242, 514
BseDI CCNNGG 2 cut(s) 193, 241
BseGI GGATG 3 cut(s) 154, 331, 351
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 3 cut(s) 79, 227, 288
BseNI ACTGG 1 cut(s) 470
BshFI GGCC 3 cut(s) 218, 240, 253
BsiHKAI GWGCWC 2 cut(s) 400, 447
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 2 cut(s) 86, 525
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 2 cut(s) 229, 298
BsnI GGCC 3 cut(s) 218, 240, 253
Bsp1286I GDGCHC 4 cut(s) 24, 59, 400, 447
BspANI GGCC 3 cut(s) 218, 240, 253
BspCNI CTCAG 3 cut(s) 78, 226, 289
BspTI CTTAAG 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 193, 241
BssNI GRCGYC 1 cut(s) 382
Bst2UI CCWGG 2 cut(s) 242, 514
Bst4CI ACNGT 1 cut(s) 395
BstACI GRCGYC 1 cut(s) 382
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 168, 251
BstDEI CTNAG 3 cut(s) 65, 213, 297
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 3 cut(s) 154, 331, 351
BstMAI GTCTC 2 cut(s) 86, 525
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 2 cut(s) 242, 514
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 240, 512
BsuRI GGCC 3 cut(s) 218, 240, 253
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 3 cut(s) 154, 331, 351
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 2 cut(s) 477, 541
Cac8I GCNNGC 2 cut(s) 168, 251
CseI GACGC 2 cut(s) 255, 390
Csp6I GTAC 2 cut(s) 10, 207
CviAII CATG 3 cut(s) 401, 421, 432
CviQI GTAC 2 cut(s) 10, 207
DdeI CTNAG 3 cut(s) 65, 213, 297
EaeI YGGCCR 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 3 cut(s) 24, 59, 447
Eco32I GATATC 1 cut(s) 140
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 240, 512
EcoRV GATATC 1 cut(s) 140
EcoT38I GRGCYC 3 cut(s) 24, 59, 447
FaeI CATG 3 cut(s) 404, 424, 435
FaiI YATR 5 cut(s) 391, 402, 418, 422, 433
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FaqI GGGAC 1 cut(s) 258
FatI CATG 3 cut(s) 400, 420, 431
FokI GGATG 3 cut(s) 161, 338, 338
FriOI GRGCYC 3 cut(s) 24, 59, 447
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 3 cut(s) 218, 240, 253
HgaI GACGC 2 cut(s) 255, 390
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 3 cut(s) 404, 424, 435
HincII GTYRAC 2 cut(s) 331, 361
HindII GTYRAC 2 cut(s) 331, 361
HinfI GANTC 3 cut(s) 332, 408, 488
HphI GGTGA 2 cut(s) 16, 439
Hpy166II GTNNAC 3 cut(s) 160, 331, 361
Hpy188I TCNGA 4 cut(s) 61, 68, 182, 457
Hpy188III TCNNGA 3 cut(s) 79, 109, 143
Hpy8I GTNNAC 3 cut(s) 160, 331, 361
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 3 cut(s) 65, 213, 297
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 3 cut(s) 404, 424, 435
MaeII ACGT 1 cut(s) 184
MboII GAAGA 4 cut(s) 124, 417, 476, 487
MfeI CAATTG 1 cut(s) 494
MhlI GDGCHC 4 cut(s) 24, 59, 400, 447
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 2 cut(s) 326, 482
MnlI CCTC 2 cut(s) 74, 139
Mox20I TGGCCA 1 cut(s) 240
MscI TGGCCA 1 cut(s) 240
MseI TTAA 2 cut(s) 126, 288
MslI CAYNNNNRTG 2 cut(s) 234, 430
Msp20I TGGCCA 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 242, 514
MunI CAATTG 1 cut(s) 494
Mva1269I GAATGC 2 cut(s) 229, 298
MvaI CCWGG 2 cut(s) 242, 514
MwoI GCNNNNNNNGC 1 cut(s) 246
NlaIII CATG 3 cut(s) 404, 424, 435
NspI RCATGY 1 cut(s) 424
PciI ACATGT 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 2 cut(s) 229, 298
PfeI GAWTC 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 512
PleI GAGTC 2 cut(s) 326, 482
PpsI GAGTC 2 cut(s) 326, 482
PscI ACATGT 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 240, 512
PspGI CCWGG 2 cut(s) 240, 512
RsaI GTAC 2 cut(s) 11, 208
RsaNI GTAC 2 cut(s) 10, 207
RseI CAYNNNNRTG 2 cut(s) 234, 430
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 2 cut(s) 126, 288
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 326, 482
ScrFI CCNGG 2 cut(s) 242, 514
SduI GDGCHC 4 cut(s) 24, 59, 400, 447
SetI ASST 6 cut(s) 66, 187, 214, 447, 452, 529
SmiMI CAYNNNNRTG 2 cut(s) 234, 430
SmlI CTYRAG 2 cut(s) 287, 522
SmoI CTYRAG 2 cut(s) 287, 522
Sse9I AATT 1 cut(s) 494
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 240, 512
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 187
TasI AATT 1 cut(s) 494
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 408
Tru1I TTAA 2 cut(s) 126, 288
Tru9I TTAA 2 cut(s) 126, 288
TscAI CASTG 2 cut(s) 477, 541
TspDTI ATGAA 2 cut(s) 62, 262
TspRI CASTG 2 cut(s) 477, 541
Vha464I CTTAAG 1 cut(s) 287
XceI RCATGY 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.