Rh4DG304700

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001814
Physical Location & Seq
Forward (+)
52710429 .. 52712808
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG304700.1

Sequence Viewer

Length: 438 bp
ATGTCCTTGATTAAGGCAAAGCGATATCTGGAGGATGTTTTGGTTCACAAGCAAGCCATTCCATTCAGACGTTTCTGCCGTGGTGTTGGGCGTACAGCTCAGGCCAAGAACAGGCATTCCAATGGCCAGGGACGCTGGCCTGTGAAATCTGCCAAGTTCATTTTGGATTTGCTTAAGAATGCTGAGAGCAATGCTGAAGTGAAGGGTTTGGATGTTGACTCTCTTGTTGTATCTCACATCCAAGTCAACCAAGCACAGAAGCAAAGACGCCGAACATACCGTGCTCATGGAAGAATCAACCCATACATGTCATCTCCATGCCACATTGAGCTCACCTTGTCTGAAAAGGAAGAACCAGTGAAGAAAGAGCCAGACTCCCAATTGGCAACCAGCAAATCCAGGAAGTCTCAAGCTGTTCGCAGTGGTGGTTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0042592 GO:0048872 GO:0061484 GO:0065007 GO:0065008
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

145

Amino Acids

16.33

Weight (kDa)

10.35

Isoelectric Point (pI)

72.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 1 - 113 4.6e-24 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 111
AflII CTTAAG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 306
AjnI CCWGG 2 cut(s) 126, 398
AloI GAACNNNNNNTCC 2 cut(s) 101, 133
AluBI AGCT 3 cut(s) 98, 331, 413
AluI AGCT 3 cut(s) 98, 331, 413
Alw21I GWGCWC 2 cut(s) 286, 333
Alw26I GTCTC 1 cut(s) 411
AoxI GGCC 3 cut(s) 102, 124, 137
AsuHPI GGTGA 1 cut(s) 325
BalI TGGCCA 1 cut(s) 126
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 286, 333
BceAI ACGGC 1 cut(s) 63
BciT130I CCWGG 2 cut(s) 128, 400
BcoDI GTCTC 1 cut(s) 411
BfrI CTTAAG 1 cut(s) 173
Bme1390I CCNGG 2 cut(s) 128, 400
BmrFI CCNGG 2 cut(s) 128, 400
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 268
BsaJI CCNNGG 2 cut(s) 79, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 2 cut(s) 128, 400
BseDI CCNNGG 2 cut(s) 79, 127
BseGI GGATG 3 cut(s) 40, 217, 237
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 2 cut(s) 113, 174
BseNI ACTGG 1 cut(s) 356
BshFI GGCC 3 cut(s) 104, 126, 139
BsiHKAI GWGCWC 2 cut(s) 286, 333
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 144
BsmI GAATGC 2 cut(s) 115, 184
BsnI GGCC 3 cut(s) 104, 126, 139
Bsp1286I GDGCHC 2 cut(s) 286, 333
BspANI GGCC 3 cut(s) 104, 126, 139
BspCNI CTCAG 2 cut(s) 112, 175
BspTI CTTAAG 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 196
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 79, 127
BssNI GRCGYC 1 cut(s) 268
Bst2UI CCWGG 2 cut(s) 128, 400
Bst4CI ACNGT 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 268
BstAFI CTTAAG 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 54, 137
BstDEI CTNAG 2 cut(s) 99, 183
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 3 cut(s) 40, 217, 237
BstMAI GTCTC 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 2 cut(s) 128, 400
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 126, 398
BsuRI GGCC 3 cut(s) 104, 126, 139
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 3 cut(s) 40, 217, 237
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 363, 427
Cac8I GCNNGC 2 cut(s) 54, 137
CseI GACGC 2 cut(s) 141, 276
Csp6I GTAC 1 cut(s) 93
CviAII CATG 3 cut(s) 287, 307, 318
CviJI RGCY 8 cut(s) 56, 98, 104, 126, 139, 331, 370, 413
CviKI_1 RGCY 8 cut(s) 56, 98, 104, 126, 139, 331, 370, 413
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 2 cut(s) 99, 183
EaeI YGGCCR 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 126, 398
EcoRV GATATC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 333
FaeI CATG 3 cut(s) 290, 310, 321
FaiI YATR 5 cut(s) 277, 288, 304, 308, 319
FaqI GGGAC 1 cut(s) 144
FatI CATG 3 cut(s) 286, 306, 317
FokI GGATG 3 cut(s) 47, 224, 224
FriOI GRGCYC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 3 cut(s) 104, 126, 139
HgaI GACGC 2 cut(s) 141, 276
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 3 cut(s) 290, 310, 321
HincII GTYRAC 2 cut(s) 217, 247
HindII GTYRAC 2 cut(s) 217, 247
HinfI GANTC 3 cut(s) 218, 294, 374
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 3 cut(s) 46, 217, 247
Hpy188I TCNGA 2 cut(s) 68, 343
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 3 cut(s) 46, 217, 247
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 2 cut(s) 99, 183
HpySE526I ACGT 1 cut(s) 70
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 3 cut(s) 290, 310, 321
MaeII ACGT 1 cut(s) 70
MboII GAAGA 3 cut(s) 303, 362, 373
MfeI CAATTG 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 286, 333
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 2 cut(s) 212, 368
MnlI CCTC 1 cut(s) 25
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 12, 174
MslI CAYNNNNRTG 2 cut(s) 120, 316
Msp20I TGGCCA 1 cut(s) 126
MspCI CTTAAG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 128, 400
MunI CAATTG 1 cut(s) 380
Mva1269I GAATGC 2 cut(s) 115, 184
MvaI CCWGG 2 cut(s) 128, 400
MwoI GCNNNNNNNGC 1 cut(s) 132
NlaIII CATG 3 cut(s) 290, 310, 321
NspI RCATGY 1 cut(s) 310
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PctI GAATGC 2 cut(s) 115, 184
PfeI GAWTC 1 cut(s) 294
PfoI TCCNGGA 1 cut(s) 398
PleI GAGTC 2 cut(s) 212, 368
PpsI GAGTC 2 cut(s) 212, 368
PscI ACATGT 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 126, 398
PspGI CCWGG 2 cut(s) 126, 398
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 2 cut(s) 120, 316
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 12, 174
SchI GAGTC 2 cut(s) 212, 368
ScrFI CCNGG 2 cut(s) 128, 400
SduI GDGCHC 2 cut(s) 286, 333
SetI ASST 5 cut(s) 73, 100, 333, 338, 415
SmiMI CAYNNNNRTG 2 cut(s) 120, 316
SmlI CTYRAG 2 cut(s) 173, 408
SmoI CTYRAG 2 cut(s) 173, 408
Sse9I AATT 1 cut(s) 380
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 126, 398
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 73
TasI AATT 1 cut(s) 380
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 12, 174
Tru9I TTAA 2 cut(s) 12, 174
TscAI CASTG 2 cut(s) 363, 427
TspDTI ATGAA 1 cut(s) 148
TspRI CASTG 2 cut(s) 363, 427
Vha464I CTTAAG 1 cut(s) 173
XceI RCATGY 1 cut(s) 310
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.