AT2G32980

HAUS augmin-like complex subunit 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003213
Physical Location & Seq
Reverse (-)
13997325 .. 13999949
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32980.1

Sequence Viewer

Length: 891 bp
ATGTCGATGGGAGGCGACACGACGTGGGTTGGGAAGAAGCCGATCAGACGCATCGGTGGATTATCTGATGCTCTCTCTATCGCCTCCGATCTCGGTTTCGCCGTCGCTCCTCCTCCGTCGCAGGAAGAATTACAAAGCTTTGCGTCTTCGAATGGGGAAAGAGGTGATGATTTGATAAGGGTATTGAGAGAGTTATCTGTTGTTCAAAGGAAAATTGCAGACTTGCAGGTCGAGCTTCAAGGGAGGAAGGATGATAAAAATGTGGCGCATTTGACTCATGTTGGTGAAATGCAAAAGAAAATCGAGACTTTGTCAAGGATTACTCAAATATTGAAAGATGTTATACAAAACAAGGATCGCATCATTGCTCGTCTCCAACAACCTTATTCGCTAGATTGCATTCCAGTTGAAGCAGAATATCAGAAACAATTCTCAGAGTTGCTAATGAAAGCCGCCAGTGATTATGGCGCCTTAACAGCATCCGTGTCTGATTTTCAGTGGAGCCAAAACTTTAAAGAACCTCCTTCAGTCTGGGGGGAAATGCTGCGTCCAATACCTGTGGCACTAGCGTCATGCACGAGATTCTTTGAGGCCATGTCTGCGATGAGAGAATCATTCGCGACTCTTCAAGAGCTTCGAGTTGGTAACTCTGCAGTTTCTTTACCAACAACACCAGGAGGTAACGAAATGACACATAGAGACTCAGATTGTGTGACTCCACCTCAAGGGAGGATAGAGTCAAGCTTCGACGATTTAGCTGTTCATAAAACAAGGAGACAAAACAATGATCAAAATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGGTAATAACAATGGGAACCGGAGACTATCTTGGCCTCCTTCTGTAAAAAAGAGTAGCGTTTAA

Protein Analysis

296

Amino Acids

33.02

Weight (kDa)

5.03

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS2 PF15003 19 - 208 4.6e-90 HAUS augmin-like complex subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 1 cut(s) 620
AciI CCGC 1 cut(s) 453
AclWI GGATC 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 468
AdeI CACNNNGTG 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 725
AgsI TTSAA 5 cut(s) 206, 239, 334, 410, 629
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 5 cut(s) 138, 235, 634, 744, 758
AluI AGCT 5 cut(s) 138, 235, 634, 744, 758
Alw26I GTCTC 5 cut(s) 299, 377, 693, 769, 844
AlwI GGATC 1 cut(s) 363
AoxI GGCC 2 cut(s) 591, 860
ApeKI GCWGC 1 cut(s) 544
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 2 cut(s) 268, 470
AsuHPI GGTGA 2 cut(s) 176, 296
AsuII TTCGAA 1 cut(s) 149
BanI GGYRCC 1 cut(s) 467
BauI CACGAG 1 cut(s) 577
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 531
BccI CCATC 1 cut(s) 821
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 675
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 5 cut(s) 299, 377, 693, 769, 844
BfaI CTAG 2 cut(s) 392, 566
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 217
BisI GCNGC 2 cut(s) 453, 545
BlsI GCNGC 2 cut(s) 454, 546
Bme1390I CCNGG 1 cut(s) 675
BmgBI CACGTC 1 cut(s) 24
BmiI GGNNCC 3 cut(s) 469, 503, 845
BmrFI CCNGG 1 cut(s) 675
BmsI GCATC 4 cut(s) 58, 60, 369, 488
BpiI GAAGAC 1 cut(s) 138
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 468
BsaWI WCCGGW 1 cut(s) 846
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse1I ACTGG 2 cut(s) 404, 456
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 1 cut(s) 675
BseGI GGATG 2 cut(s) 256, 479
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 447, 717
BseNI ACTGG 2 cut(s) 404, 456
BseRI GAGGAG 2 cut(s) 99, 102
BseXI GCAGC 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 620
BshFI GGCC 2 cut(s) 593, 862
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 847
BslI CCNNNNNNNGG 1 cut(s) 725
BsmAI GTCTC 5 cut(s) 299, 377, 693, 769, 844
BsmBI CGTCTC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 2 cut(s) 593, 862
Bsp119I TTCGAA 1 cut(s) 149
Bsp143I GATC 4 cut(s) 42, 88, 355, 787
Bsp68I TCGCGA 1 cut(s) 620
BspACI CCGC 1 cut(s) 453
BspANI GGCC 2 cut(s) 593, 862
BspCNI CTCAG 2 cut(s) 446, 716
BspFNI CGCG 1 cut(s) 620
BspLI GGNNCC 3 cut(s) 469, 503, 845
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 1 cut(s) 363
BspT104I TTCGAA 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 2 cut(s) 404, 456
BssMI GATC 4 cut(s) 42, 88, 355, 787
BssNI GRCGYC 1 cut(s) 468
BssSI CACGAG 1 cut(s) 577
Bst2BI CACGAG 1 cut(s) 577
Bst2UI CCWGG 1 cut(s) 675
Bst6I CTCTTC 1 cut(s) 630
BstACI GRCGYC 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 149
BstDEI CTNAG 2 cut(s) 433, 703
BstF5I GGATG 2 cut(s) 256, 479
BstFNI CGCG 1 cut(s) 620
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 2 cut(s) 268, 470
BstKTI GATC 4 cut(s) 45, 91, 358, 790
BstMAI GTCTC 5 cut(s) 299, 377, 693, 769, 844
BstMBI GATC 4 cut(s) 42, 88, 355, 787
BstMWI GCNNNNNNNGC 3 cut(s) 232, 476, 599
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 673
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 620
BstV1I GCAGC 1 cut(s) 531
BstV2I GAAGAC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 593, 862
BtgZI GCGATG 1 cut(s) 617
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 256, 479
BtsIMutI CAGTG 2 cut(s) 463, 503
BtuMI TCGCGA 1 cut(s) 620
BveI ACCTGC 1 cut(s) 217
CfoI GCGC 2 cut(s) 268, 470
CseI GACGC 4 cut(s) 57, 132, 536, 558
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviAII CATG 3 cut(s) 278, 573, 595
DdeI CTNAG 2 cut(s) 433, 703
DinI GGCGCC 1 cut(s) 469
DpnI GATC 4 cut(s) 44, 90, 357, 789
DpnII GATC 4 cut(s) 42, 88, 355, 787
DraI TTTAAA 1 cut(s) 514
DraIII CACNNNGTG 1 cut(s) 24
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 673
EgeI GGCGCC 1 cut(s) 469
EheI GGCGCC 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 3 cut(s) 281, 576, 598
FaiI YATR 7 cut(s) 279, 344, 465, 574, 596, 696, 765
FatI CATG 3 cut(s) 277, 572, 594
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 453, 545
FokI GGATG 2 cut(s) 263, 466
Fsp4HI GCNGC 2 cut(s) 453, 545
FspBI CTAG 2 cut(s) 392, 566
GlaI GCGC 2 cut(s) 267, 469
GluI GCNGC 2 cut(s) 453, 545
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 2 cut(s) 593, 862
HapII CCGG 1 cut(s) 847
HgaI GACGC 4 cut(s) 57, 132, 536, 558
HhaI GCGC 2 cut(s) 268, 470
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 3 cut(s) 281, 576, 598
Hin6I GCGC 2 cut(s) 266, 468
HinP1I GCGC 2 cut(s) 266, 468
HindIII AAGCTT 2 cut(s) 136, 742
HinfI GANTC 7 cut(s) 274, 582, 611, 622, 701, 715, 737
HpaII CCGG 1 cut(s) 847
HphI GGTGA 2 cut(s) 176, 296
Hpy188I TCNGA 7 cut(s) 47, 67, 88, 423, 436, 490, 706
Hpy188III TCNNGA 3 cut(s) 304, 619, 629
Hpy99I CGWCG 4 cut(s) 25, 107, 121, 752
HpyAV CCTTC 3 cut(s) 241, 534, 876
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 6 cut(s) 218, 226, 292, 399, 576, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 476, 599
HpyF3I CTNAG 2 cut(s) 433, 703
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 3 cut(s) 281, 576, 598
HspAI GCGC 2 cut(s) 266, 468
KasI GGCGCC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 4 cut(s) 42, 88, 355, 787
LmnI GCTCC 2 cut(s) 112, 501
LpnPI CCDG 9 cut(s) 107, 212, 417, 469, 517, 570, 660, 687, 860
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 4 cut(s) 58, 60, 369, 488
MaeI CTAG 2 cut(s) 392, 566
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 644, 680, 712
MalI GATC 4 cut(s) 44, 90, 357, 789
MboI GATC 4 cut(s) 42, 88, 355, 787
MluCI AATT 3 cut(s) 128, 213, 428
Mly113I GGCGCC 1 cut(s) 468
MlyI GAGTC 5 cut(s) 268, 616, 695, 709, 746
MmeI TCCRAC 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 3 cut(s) 473, 513, 889
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 1 cut(s) 847
MspR9I CCNGG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 1 cut(s) 675
MvnI CGCG 1 cut(s) 620
MwoI GCNNNNNNNGC 3 cut(s) 232, 476, 599
NarI GGCGCC 1 cut(s) 468
NdeII GATC 4 cut(s) 42, 88, 355, 787
NlaIII CATG 3 cut(s) 281, 576, 598
NlaIV GGNNCC 3 cut(s) 469, 503, 845
NmuCI GTSAC 1 cut(s) 712
NruI TCGCGA 1 cut(s) 620
NspV TTCGAA 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 582, 611
PflFI GACNNNGTC 1 cut(s) 310
PkrI GCNGC 2 cut(s) 454, 546
PleI GAGTC 5 cut(s) 268, 616, 695, 709, 745
PluTI GGCGCC 1 cut(s) 471
PpsI GAGTC 5 cut(s) 268, 616, 695, 709, 745
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 3 cut(s) 469, 503, 845
PstI CTGCAG 1 cut(s) 655
PsyI GACNNNGTC 1 cut(s) 310
RruI TCGCGA 1 cut(s) 620
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 3 cut(s) 473, 513, 889
SatI GCNGC 2 cut(s) 453, 545
Sau3AI GATC 4 cut(s) 42, 88, 355, 787
SchI GAGTC 5 cut(s) 268, 616, 695, 709, 746
ScrFI CCNGG 1 cut(s) 675
SfaNI GCATC 4 cut(s) 58, 60, 369, 488
SfcI CTRYAG 1 cut(s) 651
SfoI GGCGCC 1 cut(s) 469
SfuI TTCGAA 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
Sse9I AATT 3 cut(s) 128, 213, 428
SsiI CCGC 1 cut(s) 453
SspDI GGCGCC 1 cut(s) 467
SspI AATATT 1 cut(s) 330
SspMI CTAG 2 cut(s) 392, 566
StyD4I CCNGG 1 cut(s) 673
TaiI ACGT 1 cut(s) 26
TaqI TCGA 6 cut(s) 5, 149, 231, 303, 637, 747
TasI AATT 3 cut(s) 128, 213, 428
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 582, 611
Tru1I TTAA 3 cut(s) 473, 513, 889
Tru9I TTAA 3 cut(s) 473, 513, 889
TscAI CASTG 2 cut(s) 463, 503
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 3 cut(s) 461, 752, 810
TspGWI ACGGA 2 cut(s) 105, 472
TspRI CASTG 2 cut(s) 463, 503
Tth111I GACNNNGTC 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 2 cut(s) 392, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.