RLG00000029893

HAUS augmin-like complex subunit 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003213
Physical Location & Seq
Reverse (-)
50925075 .. 50927644
2570 bp
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UTR
Exon/CDS
Intron
RLM00000029893

Sequence Viewer

Length: 657 bp
ATGTCCGATGCTCTCTCTGTTGCTTCAGATCTTGGTTTCTCAGTTGCTACTGCTCCAACTCCGGAAGAGCTTCAGAACTGGTCAAGTAGTACTGGTGAGAAAGGTGATGACTTGATAAGGGTTCTGAGGGAACTTACCAGTGTGCAAAGGAAAATAGCAGATCTGCAAGTTGAACTTCAAGGGAGGAAAGATGATAAAAATGTATCACATTTGACAAATGTGAGTGAAATGGAAAAGAAGTGTGAAACTCTATCAAGGATTACTACAATATTAACAGATGTCATTCAGAACAAGGATCGTATCATTGCTCGTCTCCAACAACCATATTCACTAAATTGGATTCCAGTGGAAGCAGAGTATCAGAAACAATTCTCTGAATTACTGATGAAGGCTGCTAGTGATTATGGAGCATTAACAGCATCAGTTGCCGATTTCCAGTGGAGCCAGACGTTTAAGGAGCCTCCTTCAGTATGGGGGGAAATGCTTCGTCCAATTGCATGCACCCAGTTTTTCGAAGCAATGTCTGCTATAAGAGAATCGTTTGCAACACTCCAACATCTCAGAGCAGGTCATTCTAATACCGCTTCTCCAATCACACTAGCCAAGGATTCCAGAAGAGTACCAGGGGTTTCTGATTGTGTAACTCCACCTCCGTGA

Protein Analysis

219

Amino Acids

24.22

Weight (kDa)

5.3

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS2 PF15003 1 - 183 2.2e-78 HAUS augmin-like complex subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 557
AccIII TCCGGA 1 cut(s) 61
AciI CCGC 1 cut(s) 582
AclWI GGATC 1 cut(s) 303
AcuI CTGAAG 3 cut(s) 9, 56, 450
AfaI GTAC 2 cut(s) 91, 621
AgsI TTSAA 2 cut(s) 173, 179
AjnI CCWGG 1 cut(s) 622
AleI CACNNNNGTG 1 cut(s) 652
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 317
AlwI GGATC 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 61
ApeKI GCWGC 1 cut(s) 392
Asp700I GAANNNNTTC 3 cut(s) 69, 368, 483
AsuHPI GGTGA 2 cut(s) 107, 116
AsuII TTCGAA 1 cut(s) 513
BbvI GCAGC 1 cut(s) 379
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 1 cut(s) 317
BfaI CTAG 2 cut(s) 396, 599
BfuAI ACCTGC 1 cut(s) 557
BglII AGATCT 2 cut(s) 28, 160
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 624
BmiI GGNNCC 2 cut(s) 443, 459
BmrFI CCNGG 1 cut(s) 624
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 428
BmuI ACTGGG 1 cut(s) 499
Bpu14I TTCGAA 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 603, 623
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 3 cut(s) 571, 601, 634
Bse1I ACTGG 6 cut(s) 83, 97, 138, 344, 436, 505
Bse3DI GCAATG 2 cut(s) 303, 525
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 603, 623
BseMI GCAATG 2 cut(s) 303, 525
BseMII CTCAG 3 cut(s) 54, 116, 574
BseNI ACTGG 6 cut(s) 83, 97, 138, 344, 436, 505
BseXI GCAGC 1 cut(s) 379
BsiSI CCGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 317
BsmBI CGTCTC 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 513
Bsp13I TCCGGA 1 cut(s) 61
Bsp143I GATC 3 cut(s) 28, 160, 295
BspACI CCGC 1 cut(s) 582
BspCNI CTCAG 3 cut(s) 53, 117, 573
BspEI TCCGGA 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 443, 459
BspMI ACCTGC 1 cut(s) 557
BspPI GGATC 1 cut(s) 303
BspQI GCTCTTC 1 cut(s) 60
BspT104I TTCGAA 1 cut(s) 513
BsrDI GCAATG 2 cut(s) 303, 525
BsrI ACTGG 6 cut(s) 83, 97, 138, 344, 436, 505
BssECI CCNNGG 2 cut(s) 603, 623
BssMI GATC 3 cut(s) 28, 160, 295
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 624
Bst6I CTCTTC 2 cut(s) 60, 610
BstAPI GCANNNNNTGC 2 cut(s) 425, 524
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 40, 125, 560
BstKTI GATC 3 cut(s) 31, 163, 298
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 3 cut(s) 28, 160, 295
BstMWI GCNNNNNNNGC 3 cut(s) 416, 425, 524
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 501
BstSCI CCNGG 1 cut(s) 622
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 28, 160
BstYI RGATCY 2 cut(s) 28, 160
BtsIMutI CAGTG 3 cut(s) 145, 351, 443
BveI ACCTGC 1 cut(s) 557
Cac8I GCNNGC 1 cut(s) 499
Csp6I GTAC 2 cut(s) 90, 620
CviAII CATG 1 cut(s) 498
CviJI RGCY 5 cut(s) 70, 392, 444, 460, 602
CviKI_1 RGCY 5 cut(s) 70, 392, 444, 460, 602
CviQI GTAC 2 cut(s) 90, 620
DdeI CTNAG 3 cut(s) 40, 125, 560
DpnI GATC 3 cut(s) 30, 162, 297
DpnII GATC 3 cut(s) 28, 160, 295
Eam1104I CTCTTC 2 cut(s) 60, 610
EarI CTCTTC 2 cut(s) 60, 610
Eco130I CCWWGG 1 cut(s) 603
Eco57I CTGAAG 3 cut(s) 9, 56, 450
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 1 cut(s) 501
FaiI YATR 5 cut(s) 325, 405, 472, 499, 530
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 1 cut(s) 497
Fnu4HI GCNGC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 2 cut(s) 396, 599
GluI GCNGC 1 cut(s) 393
HapII CCGG 1 cut(s) 62
Hin1II CATG 1 cut(s) 501
HinfI GANTC 3 cut(s) 340, 536, 608
HpaII CCGG 1 cut(s) 62
HphI GGTGA 2 cut(s) 107, 116
Hpy188I TCNGA 9 cut(s) 7, 28, 75, 126, 288, 363, 376, 563, 634
Hpy188III TCNNGA 2 cut(s) 62, 612
HpyAV CCTTC 2 cut(s) 382, 474
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 5 cut(s) 145, 166, 497, 501, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 425, 524
HpyF3I CTNAG 3 cut(s) 40, 125, 560
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 1 cut(s) 501
Kpn2I TCCGGA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 28, 160, 295
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 4 cut(s) 58, 407, 441, 457
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 428
MaeI CTAG 2 cut(s) 396, 599
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 1 cut(s) 640
MalI GATC 3 cut(s) 30, 162, 297
MboI GATC 3 cut(s) 28, 160, 295
MboII GAAGA 2 cut(s) 77, 627
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 2 cut(s) 28, 160
MluCI AATT 4 cut(s) 334, 368, 377, 492
MmeI TCCRAC 3 cut(s) 80, 340, 577
MnlI CCTC 3 cut(s) 120, 177, 471
MroI TCCGGA 1 cut(s) 61
MroXI GAANNNNTTC 3 cut(s) 69, 368, 483
MseI TTAA 3 cut(s) 272, 413, 453
MslI CAYNNNNRTG 1 cut(s) 652
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 624
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 3 cut(s) 416, 425, 524
NdeII GATC 3 cut(s) 28, 160, 295
NlaIII CATG 1 cut(s) 501
NlaIV GGNNCC 2 cut(s) 443, 459
NspI RCATGY 1 cut(s) 501
NspV TTCGAA 1 cut(s) 513
OliI CACNNNNGTG 1 cut(s) 652
PaeI GCATGC 1 cut(s) 501
PciSI GCTCTTC 1 cut(s) 60
PdmI GAANNNNTTC 3 cut(s) 69, 368, 483
PfeI GAWTC 3 cut(s) 340, 536, 608
PkrI GCNGC 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 2 cut(s) 443, 459
PsuI RGATCY 2 cut(s) 28, 160
RsaI GTAC 2 cut(s) 91, 621
RsaNI GTAC 2 cut(s) 90, 620
RseI CAYNNNNRTG 1 cut(s) 652
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 272, 413, 453
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 3 cut(s) 28, 160, 295
ScaI AGTACT 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 624
SetI ASST 5 cut(s) 72, 106, 452, 571, 652
SfaNI GCATC 1 cut(s) 428
SfuI TTCGAA 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 652
SphI GCATGC 1 cut(s) 501
Sse9I AATT 4 cut(s) 334, 368, 377, 492
SsiI CCGC 1 cut(s) 582
SspI AATATT 1 cut(s) 270
SspMI CTAG 2 cut(s) 396, 599
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 603
TaiI ACGT 1 cut(s) 452
TaqI TCGA 1 cut(s) 513
TasI AATT 4 cut(s) 334, 368, 377, 492
TatI WGTACW 1 cut(s) 89
TfiI GAWTC 3 cut(s) 340, 536, 608
Tru1I TTAA 3 cut(s) 272, 413, 453
Tru9I TTAA 3 cut(s) 272, 413, 453
TscAI CASTG 3 cut(s) 145, 351, 443
TseI GCWGC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 3 cut(s) 145, 351, 443
XceI RCATGY 1 cut(s) 501
XmnI GAANNNNTTC 3 cut(s) 69, 368, 483
XspI CTAG 2 cut(s) 396, 599
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.