RchiOBHm_Chr2g0158271

HAUS augmin-like complex subunit 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003213
Physical Location & Seq
Reverse (-)
74623286 .. 74626924
3639 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52697

Sequence Viewer

Length: 882 bp
ATGTCGATGGGGAGTGATAGTACCTGGGTGGGGAAGAAGCCTCTGAGACGAATCGGAGGCATGTCCGATGCTCTCTCTATTGCTTCGGATCTTGGTTTCTCAGTCGCCGCTGCTCCCACTCCGGAAGAGCTTCAGCGCTGGTCGAGTAGTACTGGTGAGAAAGGTGATGACTTGATAAGGGTTCTGAGGGAACTTACCAGCGTGCAAAGGAAAATAGCAGATCTGCAAGTTGAACTTCAAGGGAGAAAAGATGATAAAAATGTATCACATTTGACACATGTGAGCGAAATGGAAAAGAAGTGTGAAACTCTATCGAGGATTACTACGATTCTAAAAGACGTCATTCAGAACAAGGATCGTATCATTGCTCGTCTCCAACAACCATATTCACTCGATTGTATTCCAGTGGAAGCAGAGTATCAGAAACAATTCTCTGAATTACTGATGAAGGCTGCTAGTGATTATGGAGCATTAACAGCTTCAGTTGCCGATTTCCAGTGGAGCCAGACATTTAAGGAGCCTCCTTCAGTATGGGGGGAAATGCTTCGTCCAATTCCTGTTGCCTTAGCATCATGCACCAGGTTTTTCGAAGCAATGTCTGCTATGAGGGAGTCGTTTGCAACACTTCAACATCTCAGAGTAGGTCATTCTGATACCTCTTCTCCTATCACACCAGCCAAGGATTCCAGAAGAGTACCAGGGGTTTCCGATTGTGTAACTCCGCCTCCCTGGAAAACTGAACCAAGCTTTGATGAAGTGGCTATCAAAAGCTCAAGAAGGACGGATATCAAGCATCAAGAAGCAGAAAATGAAAGTGAGCTGGACGGCTCTAGCCACAGGAGATTGTCCTGGCCTCCTTCAGTTAAAAAGGCTGCTACTTAA

Protein Analysis

293

Amino Acids

32.6

Weight (kDa)

6.62

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS2 PF15003 19 - 208 1.4e-89 HAUS augmin-like complex subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 2 cut(s) 108, 722
AclWI GGATC 2 cut(s) 96, 363
AcuI CTGAAG 4 cut(s) 116, 465, 510, 843
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 3 cut(s) 22, 151, 696
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 3 cut(s) 233, 239, 629
AjnI CCWGG 5 cut(s) 23, 578, 697, 728, 848
AluBI AGCT 5 cut(s) 130, 479, 747, 771, 820
AluI AGCT 5 cut(s) 130, 479, 747, 771, 820
Alw26I GTCTC 2 cut(s) 40, 377
AlwI GGATC 2 cut(s) 96, 363
Aor13HI TCCGGA 1 cut(s) 121
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 1 cut(s) 851
ApeKI GCWGC 3 cut(s) 110, 452, 872
Asp700I GAANNNNTTC 3 cut(s) 129, 428, 543
AspLEI GCGC 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 167, 176
AsuII TTCGAA 1 cut(s) 588
BbvI GCAGC 3 cut(s) 97, 439, 859
BceAI ACGGC 1 cut(s) 841
BciT130I CCWGG 5 cut(s) 25, 580, 699, 730, 850
BcoDI GTCTC 2 cut(s) 40, 377
BfaI CTAG 2 cut(s) 456, 831
BfoI RGCGCY 1 cut(s) 139
BglII AGATCT 1 cut(s) 220
BisI GCNGC 4 cut(s) 108, 111, 453, 873
BlsI GCNGC 4 cut(s) 109, 112, 454, 874
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 5 cut(s) 25, 580, 699, 730, 850
BmiI GGNNCC 2 cut(s) 503, 519
BmrFI CCNGG 5 cut(s) 25, 580, 699, 730, 850
BmsI GCATC 3 cut(s) 58, 578, 802
Bpu10I CCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 757
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 24, 678, 698, 728
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 5 cut(s) 34, 646, 676, 709, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 3 cut(s) 157, 404, 496
Bse3DI GCAATG 2 cut(s) 363, 600
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 5 cut(s) 25, 580, 699, 730, 850
BseDI CCNNGG 4 cut(s) 24, 678, 698, 728
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 2 cut(s) 363, 600
BseMII CTCAG 4 cut(s) 35, 114, 176, 649
BseNI ACTGG 3 cut(s) 157, 404, 496
BseXI GCAGC 3 cut(s) 97, 439, 859
BshFI GGCC 1 cut(s) 853
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 40, 377
BsmBI CGTCTC 2 cut(s) 40, 377
BsnI GGCC 1 cut(s) 853
Bsp119I TTCGAA 1 cut(s) 588
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 3 cut(s) 88, 220, 355
BspACI CCGC 2 cut(s) 108, 722
BspANI GGCC 1 cut(s) 853
BspCNI CTCAG 4 cut(s) 36, 113, 177, 648
BspEI TCCGGA 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 503, 519
BspPI GGATC 2 cut(s) 96, 363
BspQI GCTCTTC 1 cut(s) 120
BspT104I TTCGAA 1 cut(s) 588
BsrDI GCAATG 2 cut(s) 363, 600
BsrI ACTGG 3 cut(s) 157, 404, 496
BssECI CCNNGG 4 cut(s) 24, 678, 698, 728
BssMI GATC 3 cut(s) 88, 220, 355
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 678
Bst2UI CCWGG 5 cut(s) 25, 580, 699, 730, 850
Bst6I CTCTTC 3 cut(s) 120, 664, 685
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 5 cut(s) 44, 100, 185, 565, 635
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 3 cut(s) 91, 223, 358
BstMAI GTCTC 2 cut(s) 40, 377
BstMBI GATC 3 cut(s) 88, 220, 355
BstMWI GCNNNNNNNGC 3 cut(s) 476, 485, 599
BstNI CCWGG 5 cut(s) 25, 580, 699, 730, 850
BstNSI RCATGY 2 cut(s) 64, 281
BstSCI CCNGG 5 cut(s) 23, 578, 697, 728, 848
BstV1I GCAGC 3 cut(s) 97, 439, 859
BstX2I RGATCY 2 cut(s) 88, 220
BstYI RGATCY 2 cut(s) 88, 220
BsuRI GGCC 1 cut(s) 853
BtsIMutI CAGTG 2 cut(s) 411, 503
Cac8I GCNNGC 1 cut(s) 203
CfoI GCGC 1 cut(s) 138
CsiI ACCWGGT 1 cut(s) 578
Csp6I GTAC 3 cut(s) 21, 150, 695
CviAII CATG 3 cut(s) 61, 278, 573
CviQI GTAC 3 cut(s) 21, 150, 695
DdeI CTNAG 5 cut(s) 44, 100, 185, 565, 635
DpnI GATC 3 cut(s) 90, 222, 357
DpnII GATC 3 cut(s) 88, 220, 355
Eam1104I CTCTTC 3 cut(s) 120, 664, 685
EarI CTCTTC 3 cut(s) 120, 664, 685
EciI GGCGGA 1 cut(s) 711
Eco130I CCWWGG 1 cut(s) 678
Eco32I GATATC 1 cut(s) 787
Eco47III AGCGCT 1 cut(s) 137
Eco57I CTGAAG 4 cut(s) 116, 465, 510, 843
EcoRII CCWGG 5 cut(s) 23, 578, 697, 728, 848
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
Esp3I CGTCTC 2 cut(s) 40, 377
FaeI CATG 3 cut(s) 64, 281, 576
FaiI YATR 7 cut(s) 62, 279, 385, 465, 532, 574, 605
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FatI CATG 3 cut(s) 60, 277, 572
Fnu4HI GCNGC 4 cut(s) 108, 111, 453, 873
Fsp4HI GCNGC 4 cut(s) 108, 111, 453, 873
FspBI CTAG 2 cut(s) 456, 831
GlaI GCGC 1 cut(s) 137
GluI GCNGC 4 cut(s) 108, 111, 453, 873
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 1 cut(s) 853
HapII CCGG 1 cut(s) 122
HhaI GCGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 3 cut(s) 64, 281, 576
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 745
HinfI GANTC 4 cut(s) 51, 328, 611, 683
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 167, 176
Hpy188III TCNNGA 4 cut(s) 122, 687, 774, 797
HpyAV CCTTC 4 cut(s) 442, 534, 771, 867
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 4 cut(s) 205, 226, 576, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 485, 599
HpyF3I CTNAG 5 cut(s) 44, 100, 185, 565, 635
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 3 cut(s) 64, 281, 576
HspAI GCGC 1 cut(s) 136
Kpn2I TCCGGA 1 cut(s) 121
Kzo9I GATC 3 cut(s) 88, 220, 355
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 4 cut(s) 118, 467, 501, 517
Lsp1109I GCAGC 3 cut(s) 97, 439, 859
LweI GCATC 3 cut(s) 58, 578, 802
MabI ACCWGGT 1 cut(s) 578
MaeI CTAG 2 cut(s) 456, 831
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 715
MalI GATC 3 cut(s) 90, 222, 357
MboI GATC 3 cut(s) 88, 220, 355
MboII GAAGA 4 cut(s) 46, 137, 651, 702
MflI RGATCY 2 cut(s) 88, 220
MluCI AATT 3 cut(s) 428, 437, 552
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 9 cut(s) 50, 51, 180, 309, 531, 600, 667, 735, 864
MroI TCCGGA 1 cut(s) 121
MroXI GAANNNNTTC 3 cut(s) 129, 428, 543
MseI TTAA 4 cut(s) 473, 513, 864, 880
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 5 cut(s) 25, 580, 699, 730, 850
MvaI CCWGG 5 cut(s) 25, 580, 699, 730, 850
MwoI GCNNNNNNNGC 3 cut(s) 476, 485, 599
NdeII GATC 3 cut(s) 88, 220, 355
NlaIII CATG 3 cut(s) 64, 281, 576
NlaIV GGNNCC 2 cut(s) 503, 519
NspI RCATGY 2 cut(s) 64, 281
NspV TTCGAA 1 cut(s) 588
PciI ACATGT 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 120
PdmI GAANNNNTTC 3 cut(s) 129, 428, 543
PfeI GAWTC 3 cut(s) 51, 328, 683
PkrI GCNGC 4 cut(s) 109, 112, 454, 874
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PscI ACATGT 1 cut(s) 277
Psp6I CCWGG 5 cut(s) 23, 578, 697, 728, 848
PspGI CCWGG 5 cut(s) 23, 578, 697, 728, 848
PspN4I GGNNCC 2 cut(s) 503, 519
PsuI RGATCY 2 cut(s) 88, 220
RsaI GTAC 3 cut(s) 22, 151, 696
RsaNI GTAC 3 cut(s) 21, 150, 695
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 4 cut(s) 473, 513, 864, 880
SatI GCNGC 4 cut(s) 108, 111, 453, 873
Sau3AI GATC 3 cut(s) 88, 220, 355
ScaI AGTACT 1 cut(s) 151
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 5 cut(s) 25, 580, 699, 730, 850
SexAI ACCWGGT 1 cut(s) 578
SfaNI GCATC 3 cut(s) 58, 578, 802
SfuI TTCGAA 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 3 cut(s) 428, 437, 552
SsiI CCGC 2 cut(s) 108, 722
SspMI CTAG 2 cut(s) 456, 831
StyD4I CCNGG 5 cut(s) 23, 578, 697, 728, 848
StyI CCWWGG 1 cut(s) 678
TaiI ACGT 1 cut(s) 342
TaqI TCGA 5 cut(s) 5, 143, 314, 393, 588
TasI AATT 3 cut(s) 428, 437, 552
TatI WGTACW 1 cut(s) 149
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 3 cut(s) 51, 328, 683
Tru1I TTAA 4 cut(s) 473, 513, 864, 880
Tru9I TTAA 4 cut(s) 473, 513, 864, 880
TscAI CASTG 2 cut(s) 411, 503
TseI GCWGC 3 cut(s) 110, 452, 872
TspDTI ATGAA 3 cut(s) 461, 768, 825
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 2 cut(s) 411, 503
XceI RCATGY 2 cut(s) 64, 281
XmnI GAANNNNTTC 3 cut(s) 129, 428, 543
XspI CTAG 2 cut(s) 456, 831
ZraI GACGTC 1 cut(s) 340
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.