pycom17g09780

HAUS augmin-like complex subunit 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003213
Physical Location & Seq
Forward (+)
7441652 .. 7444527
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09780.1

Sequence Viewer

Length: 1098 bp
ATGGGAACGAGACGGGCTCCGTTAGCGTGTGGCCACCGCTGTATAAAGTTTGGGTCCACCCGGCCGGCCAGAGAGCATACGTTGCAAACAGGTTTGAAAATTTTCAGCGCATTGCTGTCTCTGAAAGCAAGAAGAACTCAGTGGAGAGAGACGACCAACCAAACCAAATTGTGGTGGTTGCCGGAAGGCCGTTGTTTGGGTGGCGCGGCAATGTCGATGGGGACTGACAGCACTTGGGTGGGGAAGAAGCCGCTGCGGCGCATCGGCGGCATGGCCGACGCCCTCTCCATCGCTTCGGATCTCGGCTTCTCCGTCGCCGCTCCTCCTACCCAGGAAGAGCTTCAGAACTTGTCCAGTAGTACTGGTGAAAAGGGTGATGACTTGATAAAGGTTTTGAGGGACCTTACCTCTGTTCAGAGAAAAATAGCAGATCTGCAAGTTGAACTCCAAGGGAGAAAGGATGATAAAAACGTAGCGCATTTGACACATGCGAGTGAAATGGAAAAGAAGTGTGAAACTTTGGCAAGGATTACTACCATACTGAAAGATGTGATTCAGAATAAGGATCGCATCATAGCCCGTCTTCAACAACCATATTCACTCGATTGCATTCCAGTAGAAGCAGAGTATCAGAAACAATTCTCTGAATTATTGATGAAGGCTGCTAGTGATTATGGGGCCTTGACAGCATCAGTTGCAGATTTCCAGTGGAGTCAGACATTTAAGGAGCCTCCTTCGGTTTGGGGGGAAATGCTTCGTCCAATTCCTGTGGCATTAGCATCATGCACCCGGTTCTTTGAAGCAATGTCTGCCATGAGGGAATCGTTTTCAACTCTTCAGCATCTCAGAGTTGGTCAGACTGATGCGCCTTCGCCTATAACACCAGGCAAGCAATCCTCCCGAAGAATACCAGGTGATTCTGAATGTGTAACTCCACCTCCTTGGAGAACTGAACCAAGCTTTGATGACTTAGCTATCAGAAGCTCAAGGAAGGCTGAAGTTGAGCACCGAGAAGCCGAGGATGAAAATAGTGAAATGGATGGCACTAGCCACCGGAGATTGTCATGGCCTCCGTCAGTTAAAAAGAACGGTGTTTAG

Protein Analysis

366

Amino Acids

40.65

Weight (kDa)

8.91

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS2 PF15003 89 - 278 3.2e-90 HAUS augmin-like complex subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 171
AccBSI CCGCTC 1 cut(s) 320
AccII CGCG 1 cut(s) 206
AciI CCGC 6 cut(s) 37, 206, 251, 256, 267, 318
AclWI GGATC 2 cut(s) 306, 573
AcoI YGGCCR 4 cut(s) 31, 62, 66, 273
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 3 cut(s) 326, 821, 1017
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 171, 196
AgsI TTSAA 5 cut(s) 97, 443, 587, 800, 831
AjnI CCWGG 3 cut(s) 330, 883, 910
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 4 cut(s) 340, 960, 974, 984
AluI AGCT 4 cut(s) 340, 960, 974, 984
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 3 cut(s) 4, 123, 143
AlwI GGATC 2 cut(s) 306, 573
AoxI GGCC 7 cut(s) 31, 62, 66, 187, 273, 678, 1067
ApeKI GCWGC 2 cut(s) 253, 662
ApoI RAATTY 1 cut(s) 99
Asp700I GAANNNNTTC 4 cut(s) 101, 339, 638, 753
AspLEI GCGC 5 cut(s) 110, 206, 261, 478, 868
AspS9I GGNCC 3 cut(s) 54, 400, 678
AsuC2I CCSGG 2 cut(s) 61, 790
AsuHPI GGTGA 3 cut(s) 377, 386, 926
AvaII GGWCC 2 cut(s) 54, 400
BalI TGGCCA 1 cut(s) 33
BanII GRGCYC 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 2 cut(s) 240, 649
BccI CCATC 3 cut(s) 211, 296, 1034
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 3 cut(s) 332, 885, 912
BcnI CCSGG 2 cut(s) 61, 790
BcoDI GTCTC 3 cut(s) 4, 123, 143
BfaI CTAG 2 cut(s) 666, 1047
BglI GCCNNNNNGGC 1 cut(s) 256
BglII AGATCT 1 cut(s) 430
BisI GCNGC 7 cut(s) 207, 251, 254, 257, 268, 318, 663
BlsI GCNGC 7 cut(s) 208, 252, 255, 258, 269, 319, 664
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 5 cut(s) 61, 332, 790, 885, 912
Bme18I GGWCC 2 cut(s) 54, 400
BmgT120I GGNCC 3 cut(s) 54, 400, 678
BmiI GGNNCC 5 cut(s) 18, 55, 401, 679, 729
BmrFI CCNGG 5 cut(s) 61, 332, 790, 885, 912
BmsI GCATC 6 cut(s) 270, 579, 698, 788, 850, 853
BpiI GAAGAC 1 cut(s) 575
BplI GAGNNNNNCTC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 970
BpuMI CCSGG 2 cut(s) 61, 790
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 330, 448, 941, 1017
BsaWI WCCGGW 1 cut(s) 1053
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 196
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 4 cut(s) 354, 367, 614, 706
Bse3DI GCAATG 3 cut(s) 110, 216, 810
BseBI CCWGG 3 cut(s) 332, 885, 912
BseDI CCNNGG 4 cut(s) 330, 448, 941, 1017
BseGI GGATG 3 cut(s) 466, 1027, 1045
BseLI CCNNNNNNNGG 2 cut(s) 171, 196
BseMI GCAATG 3 cut(s) 110, 216, 810
BseMII CTCAG 2 cut(s) 152, 859
BseNI ACTGG 4 cut(s) 354, 367, 614, 706
BseRI GAGGAG 1 cut(s) 312
BseX3I CGGCCG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 240, 649
Bsh1236I CGCG 1 cut(s) 206
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 7 cut(s) 33, 64, 68, 189, 275, 680, 1069
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 1008
BsiSI CCGG 5 cut(s) 61, 65, 182, 790, 1054
BslFI GGGAC 2 cut(s) 235, 413
BslI CCNNNNNNNGG 2 cut(s) 171, 196
BsmAI GTCTC 3 cut(s) 4, 123, 143
BsmBI CGTCTC 2 cut(s) 4, 143
BsmFI GGGAC 2 cut(s) 235, 413
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 7 cut(s) 33, 64, 68, 189, 275, 680, 1069
Bsp1286I GDGCHC 2 cut(s) 19, 1008
Bsp143I GATC 3 cut(s) 298, 430, 565
BspACI CCGC 6 cut(s) 37, 206, 251, 256, 267, 318
BspANI GGCC 7 cut(s) 33, 64, 68, 189, 275, 680, 1069
BspCNI CTCAG 2 cut(s) 151, 858
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 5 cut(s) 18, 55, 401, 679, 729
BspPI GGATC 2 cut(s) 306, 573
BspQI GCTCTTC 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 110, 216, 810
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 4 cut(s) 354, 367, 614, 706
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 4 cut(s) 330, 448, 941, 1017
BssMI GATC 3 cut(s) 298, 430, 565
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 2 cut(s) 448, 941
Bst2UI CCWGG 3 cut(s) 332, 885, 912
Bst4CI ACNGT 1 cut(s) 1091
Bst6I CTCTTC 2 cut(s) 330, 840
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 3 cut(s) 82, 695, 809
BstC8I GCNNGC 2 cut(s) 66, 890
BstDEI CTNAG 3 cut(s) 138, 845, 970
BstF5I GGATG 3 cut(s) 466, 1027, 1045
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 5 cut(s) 110, 206, 261, 478, 868
BstKTI GATC 3 cut(s) 301, 433, 568
BstMAI GTCTC 3 cut(s) 4, 123, 143
BstMBI GATC 3 cut(s) 298, 430, 565
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 7 cut(s) 23, 82, 256, 267, 686, 695, 809
BstNI CCWGG 3 cut(s) 332, 885, 912
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 5 cut(s) 59, 330, 788, 883, 910
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 240, 649
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 2 cut(s) 298, 430
BstXI CCANNNNNNTGG 1 cut(s) 942
BstYI RGATCY 2 cut(s) 298, 430
BstZI CGGCCG 1 cut(s) 62
BsuRI GGCC 7 cut(s) 33, 64, 68, 189, 275, 680, 1069
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 3 cut(s) 466, 1027, 1045
BtsIMutI CAGTG 2 cut(s) 146, 713
Cac8I GCNNGC 2 cut(s) 66, 890
CfoI GCGC 5 cut(s) 110, 206, 261, 478, 868
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 3 cut(s) 54, 400, 678
CseI GACGC 1 cut(s) 287
CsiI ACCWGGT 1 cut(s) 910
Csp6I GTAC 1 cut(s) 360
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 5 cut(s) 271, 488, 783, 814, 1065
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 3 cut(s) 138, 845, 970
DpnI GATC 3 cut(s) 300, 432, 567
DpnII GATC 3 cut(s) 298, 430, 565
EaeI YGGCCR 4 cut(s) 31, 62, 66, 273
EagI CGGCCG 1 cut(s) 62
Eam1104I CTCTTC 2 cut(s) 330, 840
EarI CTCTTC 2 cut(s) 330, 840
EclXI CGGCCG 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 448, 941
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 2 cut(s) 54, 400
Eco52I CGGCCG 1 cut(s) 62
Eco57I CTGAAG 3 cut(s) 326, 821, 1017
EcoO109I RGGNCCY 2 cut(s) 400, 678
EcoRII CCWGG 3 cut(s) 330, 883, 910
EcoT14I CCWWGG 2 cut(s) 448, 941
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 2 cut(s) 448, 941
Esp3I CGTCTC 2 cut(s) 4, 143
FaeI CATG 5 cut(s) 274, 491, 786, 817, 1068
FaqI GGGAC 2 cut(s) 235, 413
FatI CATG 5 cut(s) 270, 487, 782, 813, 1064
Fnu4HI GCNGC 7 cut(s) 207, 251, 254, 257, 268, 318, 663
FokI GGATG 3 cut(s) 473, 1034, 1052
FriOI GRGCYC 1 cut(s) 19
FseI GGCCGGCC 1 cut(s) 68
Fsp4HI GCNGC 7 cut(s) 207, 251, 254, 257, 268, 318, 663
FspBI CTAG 2 cut(s) 666, 1047
GlaI GCGC 5 cut(s) 109, 205, 260, 477, 867
GluI GCNGC 7 cut(s) 207, 251, 254, 257, 268, 318, 663
HaeIII GGCC 7 cut(s) 33, 64, 68, 189, 275, 680, 1069
HapII CCGG 5 cut(s) 61, 65, 182, 790, 1054
HgaI GACGC 1 cut(s) 287
HhaI GCGC 5 cut(s) 110, 206, 261, 478, 868
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 5 cut(s) 274, 491, 786, 817, 1068
Hin6I GCGC 5 cut(s) 108, 204, 259, 476, 866
HinP1I GCGC 5 cut(s) 108, 204, 259, 476, 866
HindIII AAGCTT 1 cut(s) 958
HinfI GANTC 4 cut(s) 553, 712, 821, 917
HpaII CCGG 5 cut(s) 61, 65, 182, 790, 1054
HphI GGTGA 3 cut(s) 377, 386, 926
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 900
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 2 cut(s) 281, 317
HpyAV CCTTC 5 cut(s) 179, 652, 744, 879, 985
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 2 cut(s) 80, 471
HpyCH4V TGCA 5 cut(s) 85, 436, 609, 698, 786
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 82, 256, 267, 686, 695, 809
HpyF3I CTNAG 3 cut(s) 138, 845, 970
HpySE526I ACGT 2 cut(s) 80, 471
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 5 cut(s) 274, 491, 786, 817, 1068
HspAI GCGC 5 cut(s) 108, 204, 259, 476, 866
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 298, 430, 565
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 3 cut(s) 22, 325, 727
Lsp1109I GCAGC 2 cut(s) 240, 649
LweI GCATC 6 cut(s) 270, 579, 698, 788, 850, 853
MabI ACCWGGT 1 cut(s) 910
MaeI CTAG 2 cut(s) 666, 1047
MaeII ACGT 2 cut(s) 80, 471
MaeIII GTNAC 1 cut(s) 928
MalI GATC 3 cut(s) 300, 432, 567
MbiI CCGCTC 1 cut(s) 320
MboI GATC 3 cut(s) 298, 430, 565
MboII GAAGA 6 cut(s) 144, 256, 347, 575, 827, 915
MflI RGATCY 2 cut(s) 298, 430
MhlI GDGCHC 2 cut(s) 19, 1008
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 5 cut(s) 99, 167, 638, 647, 762
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 1 cut(s) 721
Mox20I TGGCCA 1 cut(s) 33
MroNI GCCGGC 1 cut(s) 64
MroXI GAANNNNTTC 4 cut(s) 101, 339, 638, 753
MscI TGGCCA 1 cut(s) 33
MseI TTAA 2 cut(s) 723, 1080
MslI CAYNNNNRTG 2 cut(s) 236, 492
Msp20I TGGCCA 1 cut(s) 33
MspA1I CMGCKG 2 cut(s) 39, 253
MspI CCGG 5 cut(s) 61, 65, 182, 790, 1054
MspR9I CCNGG 5 cut(s) 61, 332, 790, 885, 912
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 3 cut(s) 332, 885, 912
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 7 cut(s) 23, 82, 256, 267, 686, 695, 809
NaeI GCCGGC 1 cut(s) 66
NciI CCSGG 2 cut(s) 61, 790
NdeII GATC 3 cut(s) 298, 430, 565
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 5 cut(s) 274, 491, 786, 817, 1068
NlaIV GGNNCC 5 cut(s) 18, 55, 401, 679, 729
NmeAIII GCCGAG 2 cut(s) 282, 1042
NspI RCATGY 1 cut(s) 491
OliI CACNNNNGTG 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 609
PdiI GCCGGC 1 cut(s) 66
PdmI GAANNNNTTC 4 cut(s) 101, 339, 638, 753
PfeI GAWTC 3 cut(s) 553, 821, 917
PflMI CCANNNNNTGG 1 cut(s) 171
PkrI GCNGC 7 cut(s) 208, 252, 255, 258, 269, 319, 664
PleI GAGTC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 720
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 330, 883, 910
PspGI CCWGG 3 cut(s) 330, 883, 910
PspN4I GGNNCC 5 cut(s) 18, 55, 401, 679, 729
PspPI GGNCC 3 cut(s) 54, 400, 678
PspPPI RGGWCCY 1 cut(s) 400
PsuI RGATCY 2 cut(s) 298, 430
RigI GGCCGGCC 1 cut(s) 68
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 2 cut(s) 236, 492
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 723, 1080
SatI GCNGC 7 cut(s) 207, 251, 254, 257, 268, 318, 663
Sau3AI GATC 3 cut(s) 298, 430, 565
Sau96I GGNCC 3 cut(s) 54, 400, 678
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 5 cut(s) 61, 332, 790, 885, 912
SduI GDGCHC 2 cut(s) 19, 1008
SexAI ACCWGGT 1 cut(s) 910
SfaNI GCATC 6 cut(s) 270, 579, 698, 788, 850, 853
SinI GGWCC 2 cut(s) 54, 400
SmiMI CAYNNNNRTG 2 cut(s) 236, 492
SmlI CTYRAG 1 cut(s) 985
SmoI CTYRAG 1 cut(s) 985
Sse9I AATT 5 cut(s) 99, 167, 638, 647, 762
SsiI CCGC 6 cut(s) 37, 206, 251, 256, 267, 318
SspMI CTAG 2 cut(s) 666, 1047
StyD4I CCNGG 5 cut(s) 59, 330, 788, 883, 910
StyI CCWWGG 2 cut(s) 448, 941
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 2 cut(s) 83, 474
TaqI TCGA 2 cut(s) 215, 603
TasI AATT 5 cut(s) 99, 167, 638, 647, 762
TatI WGTACW 1 cut(s) 359
TauI GCSGC 5 cut(s) 209, 253, 259, 270, 320
TfiI GAWTC 3 cut(s) 553, 821, 917
Tru1I TTAA 2 cut(s) 723, 1080
Tru9I TTAA 2 cut(s) 723, 1080
TscAI CASTG 2 cut(s) 146, 713
TseI GCWGC 2 cut(s) 253, 662
TspDTI ATGAA 2 cut(s) 671, 1038
TspGWI ACGGA 3 cut(s) 9, 301, 1062
TspRI CASTG 2 cut(s) 146, 713
Van91I CCANNNNNTGG 1 cut(s) 171
VpaK11BI GGWCC 2 cut(s) 54, 400
XapI RAATTY 1 cut(s) 99
XceI RCATGY 1 cut(s) 491
XmnI GAANNNNTTC 4 cut(s) 101, 339, 638, 753
XspI CTAG 2 cut(s) 666, 1047
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.