AT3G13750

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001950
Physical Location & Seq
Forward (+)
4510965 .. 4516478
5514 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13750.1

Sequence Viewer

Length: 2544 bp
ATGGGTTCGAAACCTAATGCCATGAAGAATGTGGTGGCTATGGCGGCAGTTTCTGCTCTGTTCCTTCTAGGGTTTCTTGTTTGCTCTGTTTCTGGTTCTGTCTCTTACGATAGCAGAGCCATTACCATCAATGGCAAAAGAAGAATCCTTATCTCTGGATCTATTCACTACCCCAGAAGTACTCCTGAGATGTGGCCAGATCTAATTCGGAAAGCAAAGGAAGGAGGCTTAGATGTGATTCAGACTTATGTGTTCTGGAATGGGCATGAGCCATCTCCTGGAAAATATTATTTCGAGGGCAACTATGATTTGGTTAAGTTTGTCAAGTTGGTCCAACAATCTGGACTCTATCTTCATCTAAGGATTGGTCCTTATGTCTGTGCTGAATGGAACTTCGGAGGATTTCCTGTTTGGCTTAAGTACATTCCAGGGATTAGCTTCAGAACAGATAACGGTCCTTTCAAGGCTCAAATGCAAAGATTCACCACGAAGATTGTTAACATGATGAAAGCTGAGAGGCTTTTTGAATCTCAAGGCGGTCCTATAATTCTCTCTCAGATTGAAAATGAATATGGACCAATGGAGTATGAGCTTGGTGCACCGGGCCGGTCATACACCAACTGGGCAGCTAAGATGGCGGTGGGGCTAGGCACTGGTGTCCCTTGGGTCATGTGCAAACAAGACGATGCTCCCGACCCCATTATCAATGCTTGCAATGGCTTCTACTGTGATTACTTCTCCCCAAACAAGGCTTACAAGCCTAAAATGTGGACCGAAGCCTGGACTGGATGGTTTACAAAGTTTGGAGGCCCAGTTCCTTACCGCCCAGCTGAAGATATGGCCTTTTCCGTTGCAAGGTTTATCCAAAAGGGTGGATCTTTCATCAACTACTATATGTATCATGGTGGAACAAACTTTGGTCGAACTGCTGGTGGTCCGTTTATCGCAACTAGCTATGATTATGATGCTCCTCTTGACGAATATGGACTTGAAAGGCAGCCTAAGTGGGGGCACTTGAAAGATTTGCATAGAGCAATTAAGCTGTGTGAACCAGCTTTAGTATCAGGAGAGCCCACAAGAATGCCTCTCGGAAACTATCAAGAGGCTCATGTGTACAAATCAAAATCTGGAGCTTGTTCTGCATTCTTAGCAAATTATAATCCAAAATCTTACGCCAAAGTTTCTTTCGGGAACAATCACTACAATCTCCCTCCGTGGTCTATAAGTATTCTCCCAGACTGCAAAAACACAGTTTACAACACAGCCAGGGTTGGTGCTCAAACTTCTAGGATGAAAATGGTTCGGGTTCCTGTTCATGGTGGTTTATCTTGGCAAGCATACAATGAAGACCCATCAACTTACATCGATGAGTCATTCACAATGGTCGGATTAGTGGAGCAGATAAACACTACACGAGACACTTCGGATTACCTGTGGTACATGACTGATGTTAAAGTTGATGCCAATGAAGGATTTTTGAGGAATGGAGATTTGCCGACTCTTACTGTTCTATCAGCTGGCCATGCTATGCATGTGTTTATCAATGGTCAATTATCTGGATCTGCATATGGTAGTTTAGACTCTCCTAAGCTAACTTTTCGGAAAGGTGTGAATCTAAGAGCTGGTTTCAACAAAATTGCGATACTAAGCATTGCTGTTGGTCTCCCGAATGTGGGTCCACATTTTGAGACATGGAATGCTGGAGTTTTGGGTCCAGTTTCATTGAACGGTCTCAATGGGGGACGGAGAGATCTATCATGGCAGAAATGGACTTATAAGGTTGGTCTTAAAGGAGAGTCTCTGAGTCTTCATTCACTTAGTGGTAGCTCATCGGTTGAGTGGGCAGAAGGTGCATTTGTTGCACAGAAGCAACCACTTACTTGGTACAAGACGACTTTCTCTGCTCCAGCTGGAGATTCGCCATTGGCTGTAGACATGGGAAGCATGGGAAAAGGTCAAATATGGATAAATGGGCAGAGCCTGGGACGTCACTGGCCTGCATATAAAGCAGTTGGTTCTTGCAGCGAGTGTTCTTATACCGGAACATTCAGAGAGGACAAGTGCTTAAGAAACTGTGGAGAGGCTTCTCAAAGATGGTACCATGTCCCAAGGTCGTGGCTAAAACCAAGTGGCAATCTATTGGTTGTCTTTGAGGAATGGGGAGGAGACCCGAACGGAATCACGTTGGTCAGAAGAGAAGTGGACAGTGTATGTGCAGATATCTATGAATGGCAATCAACGCTGGTGAACTACCAATTGCATGCTTCTGGAAAAGTTAATAAACCATTGCATCCCAAAGCACATTTACAATGCGGGCCCGGACAAAAGATCACCACCGTGAAGTTTGCTAGTTTCGGGACACCTGAAGGGACTTGTGGTAGTTACCGTCAAGGAAGCTGTCACGCCCATCACTCCTATGACGCTTTCAACAAACTTTGTGTTGGGCAGAACTGGTGTTCTGTAACTGTAGCACCGGAGATGTTTGGTGGAGATCCGTGTCCAAATGTGATGAAGAAACTCGCGGTGGAAGCCGTTTGTGCTTAA

Protein Analysis

847

Amino Acids

93.66

Weight (kDa)

8.8

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 41 - 345 1.6e-121 Glycosyl hydrolases family 35
GHD PF17834 368 - 424 7.9e-24 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 477 - 528 5.2e-07 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 626 - 712 3.9e-25 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 769 - 846 6.4e-22 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1158, 1776
AatII GACGTC 1 cut(s) 1990
Acc65I GGTACC 1 cut(s) 2097
AccB1I GGYRCC 1 cut(s) 2097
AccB7I CCANNNNNTGG 1 cut(s) 278
AccI GTMKAC 1 cut(s) 1932
AccII CGCG 1 cut(s) 2522
AciI CCGC 6 cut(s) 44, 537, 638, 823, 2313, 2522
AclWI GGATC 4 cut(s) 166, 883, 1567, 2486
AcoI YGGCCR 2 cut(s) 194, 1519
AcuI CTGAAG 3 cut(s) 424, 852, 2385
AcyI GRCGYC 1 cut(s) 1987
AdeI CACNNNGTG 1 cut(s) 1820
AfaI GTAC 6 cut(s) 181, 422, 1115, 1439, 1886, 2099
AfiI CCNNNNNNNGG 8 cut(s) 278, 748, 780, 855, 1007, 1316, 1672, 1673
AflII CTTAAG 2 cut(s) 416, 2065
AgsI TTSAA 8 cut(s) 463, 527, 563, 992, 1018, 1630, 1726, 2428
AjnI CCWGG 5 cut(s) 277, 427, 779, 1265, 1980
AjuI GAANNNNNNNTTGG 2 cut(s) 1169, 1201
AleI CACNNNNGTG 1 cut(s) 2336
AloI GAACNNNNNNTCC 2 cut(s) 798, 830
Alw21I GWGCWC 2 cut(s) 601, 1279
Alw26I GTCTC 7 cut(s) 106, 1410, 1667, 1682, 1736, 1803, 2160
Alw44I GTGCAC 1 cut(s) 597
AlwI GGATC 4 cut(s) 166, 883, 1567, 2486
AlwNI CAGNNNCTG 2 cut(s) 53, 1981
AoxI GGCC 7 cut(s) 194, 604, 808, 840, 1519, 1994, 2315
ApaI GGGCCC 1 cut(s) 2319
ApaLI GTGCAC 1 cut(s) 597
ApeKI GCWGC 3 cut(s) 626, 997, 2022
Asp718I GGTACC 1 cut(s) 2097
AsuC2I CCSGG 2 cut(s) 603, 2319
AsuHPI GGTGA 3 cut(s) 475, 2257, 2323
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 9 cut(s) 331, 368, 455, 539, 575, 771, 935, 1676, 1712
BaeGI GKGCMC 3 cut(s) 601, 1014, 2319
BalI TGGCCA 2 cut(s) 196, 1521
BanI GGYRCC 1 cut(s) 2097
BanII GRGCYC 2 cut(s) 1074, 2319
BauI CACGAG 1 cut(s) 1413
BbsI GAAGAC 2 cut(s) 1353, 1799
Bbv12I GWGCWC 2 cut(s) 601, 1279
BbvI GCAGC 3 cut(s) 638, 1009, 2034
BccI CCATC 7 cut(s) 134, 280, 628, 783, 1360, 2088, 2415
BceAI ACGGC 1 cut(s) 2516
BciT130I CCWGG 5 cut(s) 279, 429, 781, 1267, 1982
BcnI CCSGG 2 cut(s) 603, 2319
BcoDI GTCTC 7 cut(s) 106, 1410, 1667, 1682, 1736, 1803, 2160
BfaI CTAG 5 cut(s) 68, 647, 951, 1287, 2349
BfmI CTRYAG 2 cut(s) 1929, 2466
BfrI CTTAAG 2 cut(s) 416, 2065
BglII AGATCT 2 cut(s) 199, 1750
BisI GCNGC 4 cut(s) 45, 627, 998, 2023
BlsI GCNGC 4 cut(s) 46, 628, 999, 2024
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 7 cut(s) 279, 429, 603, 781, 1267, 1982, 2319
Bme18I GGWCC 9 cut(s) 331, 368, 455, 539, 575, 771, 935, 1676, 1712
BmiI GGNNCC 5 cut(s) 1308, 1677, 1713, 2099, 2317
BmrFI CCNGG 7 cut(s) 279, 429, 603, 781, 1267, 1982, 2319
BmrI ACTGGG 2 cut(s) 631, 806
BmsI GCATC 4 cut(s) 676, 955, 1450, 2299
BmuI ACTGGG 2 cut(s) 631, 806
BpiI GAAGAC 2 cut(s) 1353, 1799
BpmI CTGGAG 4 cut(s) 1149, 1722, 1890, 1932
Bpu10I CCTNAGC 1 cut(s) 1587
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 603, 2319
Bsa29I ATCGAT 1 cut(s) 1365
BsaHI GRCGYC 1 cut(s) 1987
BsaI GGTCTC 3 cut(s) 1667, 1736, 2160
BsaJI CCNNGG 6 cut(s) 428, 662, 1214, 1266, 1981, 2108
BsaWI WCCGGW 2 cut(s) 2039, 2473
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 8 cut(s) 278, 748, 780, 855, 1007, 1316, 1672, 1673
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 7 cut(s) 626, 658, 790, 812, 1715, 1997, 2456
Bse3DI GCAATG 3 cut(s) 721, 1650, 2285
BseBI CCWGG 5 cut(s) 279, 429, 781, 1267, 1982
BseCI ATCGAT 1 cut(s) 1365
BseDI CCNNGG 6 cut(s) 428, 662, 1214, 1266, 1981, 2108
BseGI GGATG 3 cut(s) 794, 1296, 2290
BseLI CCNNNNNNNGG 8 cut(s) 278, 748, 780, 855, 1007, 1316, 1672, 1673
BseMI GCAATG 3 cut(s) 721, 1650, 2285
BseMII CTCAG 4 cut(s) 177, 504, 569, 1793
BseNI ACTGG 7 cut(s) 626, 658, 790, 812, 1715, 1997, 2456
BseRI GAGGAG 2 cut(s) 960, 2178
BseSI GKGCMC 3 cut(s) 601, 1014, 2319
BseXI GCAGC 3 cut(s) 638, 1009, 2034
BseYI CCCAGC 1 cut(s) 826
BsgI GTGCAG 1 cut(s) 2235
Bsh1236I CGCG 1 cut(s) 2522
BshFI GGCC 7 cut(s) 196, 606, 810, 842, 1521, 1996, 2317
BshNI GGYRCC 1 cut(s) 2097
BshVI ATCGAT 1 cut(s) 1365
BsiHKAI GWGCWC 2 cut(s) 601, 1279
BsiSI CCGG 5 cut(s) 602, 607, 2040, 2319, 2474
BslFI GGGAC 6 cut(s) 644, 1755, 1998, 2090, 2371, 2383
BslI CCNNNNNNNGG 8 cut(s) 278, 748, 780, 855, 1007, 1316, 1672, 1673
BsmAI GTCTC 7 cut(s) 106, 1410, 1667, 1682, 1736, 1803, 2160
BsmFI GGGAC 6 cut(s) 644, 1755, 1998, 2090, 2371, 2383
BsmI GAATGC 3 cut(s) 1086, 1142, 1702
BsnI GGCC 7 cut(s) 196, 606, 810, 842, 1521, 1996, 2317
Bso31I GGTCTC 3 cut(s) 1667, 1736, 2160
Bsp119I TTCGAA 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 2315
Bsp1286I GDGCHC 5 cut(s) 601, 1014, 1074, 1279, 2319
Bsp1407I TGTACA 1 cut(s) 1113
Bsp143I GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
BspACI CCGC 6 cut(s) 44, 537, 638, 823, 2313, 2522
BspANI GGCC 7 cut(s) 196, 606, 810, 842, 1521, 1996, 2317
BspCNI CTCAG 4 cut(s) 178, 505, 568, 1794
BspDI ATCGAT 1 cut(s) 1365
BspFNI CGCG 1 cut(s) 2522
BspLI GGNNCC 5 cut(s) 1308, 1677, 1713, 2099, 2317
BspPI GGATC 4 cut(s) 166, 883, 1567, 2486
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 2097
BspTI CTTAAG 2 cut(s) 416, 2065
BspTNI GGTCTC 3 cut(s) 1667, 1736, 2160
BsrDI GCAATG 3 cut(s) 721, 1650, 2285
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 1113
BsrI ACTGG 7 cut(s) 626, 658, 790, 812, 1715, 1997, 2456
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 6 cut(s) 428, 662, 1214, 1266, 1981, 2108
BssMI GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
BssNI GRCGYC 1 cut(s) 1987
BssSI CACGAG 1 cut(s) 1413
BssT1I CCWWGG 2 cut(s) 662, 2108
Bst2BI CACGAG 1 cut(s) 1413
Bst2UI CCWGG 5 cut(s) 279, 429, 781, 1267, 1982
Bst6I CTCTTC 1 cut(s) 2188
BstACI GRCGYC 1 cut(s) 1987
BstAFI CTTAAG 2 cut(s) 416, 2065
BstAPI GCANNNNNTGC 3 cut(s) 53, 1850, 1859
BstAUI TGTACA 1 cut(s) 1113
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 6 cut(s) 712, 1335, 1519, 1998, 2262, 2315
BstDSI CCRYGG 1 cut(s) 1214
BstF5I GGATG 3 cut(s) 794, 1296, 2290
BstFNI CGCG 1 cut(s) 2522
BstKTI GATC 7 cut(s) 161, 202, 878, 1562, 1753, 2331, 2494
BstMAI GTCTC 7 cut(s) 106, 1410, 1667, 1682, 1736, 1803, 2160
BstMBI GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
BstNI CCWGG 5 cut(s) 279, 429, 781, 1267, 1982
BstNSI RCATGY 2 cut(s) 1535, 2264
BstSCI CCNGG 7 cut(s) 277, 427, 601, 779, 1265, 1980, 2317
BstSFI CTRYAG 2 cut(s) 1929, 2466
BstSLI GKGCMC 3 cut(s) 601, 1014, 2319
BstUI CGCG 1 cut(s) 2522
BstV1I GCAGC 3 cut(s) 638, 1009, 2034
BstV2I GAAGAC 2 cut(s) 1353, 1799
BstX2I RGATCY 6 cut(s) 158, 199, 875, 1559, 1750, 2491
BstXI CCANNNNNNTGG 4 cut(s) 341, 872, 1881, 2115
BstYI RGATCY 6 cut(s) 158, 199, 875, 1559, 1750, 2491
Bsu15I ATCGAT 1 cut(s) 1365
BsuRI GGCC 7 cut(s) 196, 606, 810, 842, 1521, 1996, 2317
BsuTUI ATCGAT 1 cut(s) 1365
BtgI CCRYGG 1 cut(s) 1214
BtsCI GGATG 3 cut(s) 794, 1296, 2290
BtsIMutI CAGTG 3 cut(s) 651, 1990, 2212
Cac8I GCNNGC 6 cut(s) 712, 1335, 1519, 1998, 2262, 2315
CaiI CAGNNNCTG 2 cut(s) 53, 1981
Cfr10I RCCGGY 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 1365
CseI GACGC 1 cut(s) 2429
Csp6I GTAC 6 cut(s) 180, 421, 1114, 1438, 1885, 2098
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviQI GTAC 6 cut(s) 180, 421, 1114, 1438, 1885, 2098
DpnI GATC 7 cut(s) 160, 201, 877, 1561, 1752, 2330, 2493
DpnII GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
DraIII CACNNNGTG 1 cut(s) 1820
EaeI YGGCCR 2 cut(s) 194, 1519
Eam1104I CTCTTC 1 cut(s) 2188
EarI CTCTTC 1 cut(s) 2188
Eco130I CCWWGG 2 cut(s) 662, 2108
Eco24I GRGCYC 2 cut(s) 1074, 2319
Eco31I GGTCTC 3 cut(s) 1667, 1736, 2160
Eco32I GATATC 1 cut(s) 2221
Eco47I GGWCC 9 cut(s) 331, 368, 455, 539, 575, 771, 935, 1676, 1712
Eco57I CTGAAG 3 cut(s) 424, 852, 2385
EcoRII CCWGG 5 cut(s) 277, 427, 779, 1265, 1980
EcoRV GATATC 1 cut(s) 2221
EcoT14I CCWWGG 2 cut(s) 662, 2108
EcoT22I ATGCAT 1 cut(s) 1533
EcoT38I GRGCYC 2 cut(s) 1074, 2319
ErhI CCWWGG 2 cut(s) 662, 2108
FaqI GGGAC 6 cut(s) 644, 1755, 1998, 2090, 2371, 2383
FauI CCCGC 1 cut(s) 2306
FauNDI CATATG 1 cut(s) 1567
FblI GTMKAC 1 cut(s) 1932
Fnu4HI GCNGC 4 cut(s) 45, 627, 998, 2023
FokI GGATG 3 cut(s) 801, 1303, 2277
FriOI GRGCYC 2 cut(s) 1074, 2319
Fsp4HI GCNGC 4 cut(s) 45, 627, 998, 2023
FspBI CTAG 5 cut(s) 68, 647, 951, 1287, 2349
GluI GCNGC 4 cut(s) 45, 627, 998, 2023
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 4 cut(s) 1149, 1722, 1890, 1932
HaeIII GGCC 7 cut(s) 196, 606, 810, 842, 1521, 1996, 2317
HapII CCGG 5 cut(s) 602, 607, 2040, 2319, 2474
HgaI GACGC 1 cut(s) 2429
Hin1I GRCGYC 1 cut(s) 1987
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HpaI GTTAAC 1 cut(s) 499
HpaII CCGG 5 cut(s) 602, 607, 2040, 2319, 2474
HphI GGTGA 3 cut(s) 475, 2257, 2323
HpyAV CCTTC 5 cut(s) 74, 215, 1463, 1841, 2360
HpyCH4IV ACGT 2 cut(s) 1987, 2183
HpySE526I ACGT 2 cut(s) 1987, 2183
Hsp92I GRCGYC 1 cut(s) 1987
KpnI GGTACC 1 cut(s) 2101
KspAI GTTAAC 1 cut(s) 499
Kzo9I GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
LmnI GCTCC 5 cut(s) 694, 973, 1130, 1396, 1909
Lsp1109I GCAGC 3 cut(s) 638, 1009, 2034
LweI GCATC 4 cut(s) 676, 955, 1450, 2299
MaeI CTAG 5 cut(s) 68, 647, 951, 1287, 2349
MaeII ACGT 2 cut(s) 1987, 2183
MaeIII GTNAC 4 cut(s) 1988, 2381, 2399, 2461
MalI GATC 7 cut(s) 160, 201, 877, 1561, 1752, 2330, 2493
MboI GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
MboII GAAGA 9 cut(s) 37, 153, 344, 502, 845, 1358, 1799, 2205, 2524
MfeI CAATTG 1 cut(s) 2255
MflI RGATCY 6 cut(s) 158, 199, 875, 1559, 1750, 2491
MhlI GDGCHC 5 cut(s) 601, 1014, 1074, 1279, 2319
MlsI TGGCCA 2 cut(s) 196, 1521
MluCI AATT 7 cut(s) 204, 546, 1035, 1153, 1550, 1635, 2255
MluNI TGGCCA 2 cut(s) 196, 1521
MlyI GAGTC 6 cut(s) 339, 1379, 1492, 1574, 1805, 1813
MmeI TCCRAC 2 cut(s) 358, 1366
Mox20I TGGCCA 2 cut(s) 196, 1521
Mph1103I ATGCAT 1 cut(s) 1533
MscI TGGCCA 2 cut(s) 196, 1521
MseI TTAA 9 cut(s) 315, 417, 498, 1038, 1452, 1788, 2066, 2277, 2542
MslI CAYNNNNRTG 3 cut(s) 1079, 2336, 2415
Msp20I TGGCCA 2 cut(s) 196, 1521
MspA1I CMGCKG 3 cut(s) 830, 1517, 1910
MspCI CTTAAG 2 cut(s) 416, 2065
MspI CCGG 5 cut(s) 602, 607, 2040, 2319, 2474
MspR9I CCNGG 7 cut(s) 279, 429, 603, 781, 1267, 1982, 2319
MunI CAATTG 1 cut(s) 2255
Mva1269I GAATGC 3 cut(s) 1086, 1142, 1702
MvaI CCWGG 5 cut(s) 279, 429, 781, 1267, 1982
MvnI CGCG 1 cut(s) 2522
NciI CCSGG 2 cut(s) 603, 2319
NdeI CATATG 1 cut(s) 1567
NdeII GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
NlaIV GGNNCC 5 cut(s) 1308, 1677, 1713, 2099, 2317
NmuCI GTSAC 2 cut(s) 1988, 2399
NsiI ATGCAT 1 cut(s) 1533
NspI RCATGY 2 cut(s) 1535, 2264
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 2336
PaeI GCATGC 1 cut(s) 2264
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PctI GAATGC 3 cut(s) 1086, 1142, 1702
PfeI GAWTC 7 cut(s) 144, 238, 480, 527, 1612, 1916, 2178
PflMI CCANNNNNTGG 1 cut(s) 278
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 4 cut(s) 46, 628, 999, 2024
PleI GAGTC 6 cut(s) 339, 1378, 1492, 1574, 1804, 1812
PpsI GAGTC 6 cut(s) 339, 1378, 1492, 1574, 1804, 1812
PsiI TTATAA 2 cut(s) 1158, 1776
Psp6I CCWGG 5 cut(s) 277, 427, 779, 1265, 1980
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 5 cut(s) 277, 427, 779, 1265, 1980
PspN4I GGNNCC 5 cut(s) 1308, 1677, 1713, 2099, 2317
PspOMI GGGCCC 1 cut(s) 2315
PsrI GAACNNNNNNTAC 2 cut(s) 1184, 1216
PstNI CAGNNNCTG 2 cut(s) 53, 1981
PsuI RGATCY 6 cut(s) 158, 199, 875, 1559, 1750, 2491
PvuII CAGCTG 3 cut(s) 830, 1517, 1910
RsaI GTAC 6 cut(s) 181, 422, 1115, 1439, 1886, 2099
RsaNI GTAC 6 cut(s) 180, 421, 1114, 1438, 1885, 2098
RseI CAYNNNNRTG 3 cut(s) 1079, 2336, 2415
SaqAI TTAA 9 cut(s) 315, 417, 498, 1038, 1452, 1788, 2066, 2277, 2542
SatI GCNGC 4 cut(s) 45, 627, 998, 2023
Sau3AI GATC 7 cut(s) 158, 199, 875, 1559, 1750, 2328, 2491
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 6 cut(s) 339, 1379, 1492, 1574, 1805, 1813
ScrFI CCNGG 7 cut(s) 279, 429, 603, 781, 1267, 1982, 2319
SduI GDGCHC 5 cut(s) 601, 1014, 1074, 1279, 2319
SfaNI GCATC 4 cut(s) 676, 955, 1450, 2299
SfcI CTRYAG 2 cut(s) 1929, 2466
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 9 cut(s) 331, 368, 455, 539, 575, 771, 935, 1676, 1712
SmiMI CAYNNNNRTG 3 cut(s) 1079, 2336, 2415
SmlI CTYRAG 3 cut(s) 416, 531, 2065
SmoI CTYRAG 3 cut(s) 416, 531, 2065
SphI GCATGC 1 cut(s) 2264
Sse9I AATT 7 cut(s) 204, 546, 1035, 1153, 1550, 1635, 2255
SsiI CCGC 6 cut(s) 44, 537, 638, 823, 2313, 2522
SspI AATATT 1 cut(s) 287
SspMI CTAG 5 cut(s) 68, 647, 951, 1287, 2349
StyD4I CCNGG 7 cut(s) 277, 427, 601, 779, 1265, 1980, 2317
StyI CCWWGG 2 cut(s) 662, 2108
TaiI ACGT 2 cut(s) 1990, 2186
TaqI TCGA 4 cut(s) 8, 294, 922, 1365
TaqII GACCGA 1 cut(s) 788
TasI AATT 7 cut(s) 204, 546, 1035, 1153, 1550, 1635, 2255
TatI WGTACW 3 cut(s) 179, 420, 1113
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 7 cut(s) 144, 238, 480, 527, 1612, 1916, 2178
Tru1I TTAA 9 cut(s) 315, 417, 498, 1038, 1452, 1788, 2066, 2277, 2542
Tru9I TTAA 9 cut(s) 315, 417, 498, 1038, 1452, 1788, 2066, 2277, 2542
TscAI CASTG 3 cut(s) 658, 1997, 2212
TseFI GTSAC 2 cut(s) 1988, 2399
TseI GCWGC 3 cut(s) 626, 997, 2022
Tsp45I GTSAC 2 cut(s) 1988, 2399
TspGWI ACGGA 6 cut(s) 838, 927, 1203, 1759, 2190, 2484
TspRI CASTG 3 cut(s) 658, 1997, 2212
Van91I CCANNNNNTGG 1 cut(s) 278
Vha464I CTTAAG 2 cut(s) 416, 2065
VneI GTGCAC 1 cut(s) 597
VpaK11BI GGWCC 9 cut(s) 331, 368, 455, 539, 575, 771, 935, 1676, 1712
XceI RCATGY 2 cut(s) 1535, 2264
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 1932
XspI CTAG 5 cut(s) 68, 647, 951, 1287, 2349
ZraI GACGTC 1 cut(s) 1988
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.