Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001950

Physical Location & Seq

Genomic Coordinates Forward (+)

26336764 .. 26344168

Gene Structure 7405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g33260.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGTTTGAAGCTTGTAATGTGGAATGTGGTGGTAGCAGCAACCCTGGTGGTATTGTGTTCTTGTTTTGCTTCTGTGAGAGCCTCTGTTTCTTATGATTCCAAGGCCATTGTCATCAATGGCCAGAGGAGGATCCTCATTTCTGGATCTATTCACTACCCAAGAAGCACCCCTGAGATGTGGCCAGATCTTATTCAGAGAGCCAAGGACGGAGGCTTGGATGTGATTCAGACTTATGTTTTCTGGAATGGGCATGAGCCTTCACCTGGCAAATACTATTTTGAGGACAACTATGATCTGGTCAAATTTATCAAGCTGGTACAGCAGGCAGGCCTTTATGTTCATCTCAGGATTGGTCCTTATGTTTGTGCAGAGTGGAACTTTGGGGGATTTCCAGTTTGGCTGAAGTACATTCCTGGTATCCAATTCAGAACAGATAATGGACCTTTCAAGGATCAAATGCAAAGATTCACAACAAAGATTGTAAACATGATGAAAGCAGAAAGGCTGTTCGAGTCTCATGGCGGTCCAATAATTCTATCCCAGATTGAAAATGAGTATGGCCCCATGGAATACGAAATTGGAGCACCAGGTAAAGCCTACACTGACTGGGCAGCTCAAATGGCTGTTGGACTTGGGACTGGTGTCCCCTGGGTCATGTGCAAACAAGATGATGCCCCTGATCCTGTTATTAACGCTTGCAATGGCTTCTATTGTGATTACTTCTCTCCAAACAAGGCTTATAAACCAAAGATGTGGACTGAAGCCTGGACTGGGTGGTTTACTGAGTTTGGAGGTGCAGTTCCTTACCGACCAGCTGAGGATTTGGCATTTTCAGTTGCAAAGTTTATACAAAAGGGTGGAGCATTCATTAACTATTATATGTATCATGGAGGAACAAATTTCGGGCGAACTGCTGGTGGTCCATTCATCGCTACTAGTTATGATTACGATGCTCCTCTGGATGAATACGGACTGTTGAGGCAACCTAAATGGGGCCATTTGAAAGATCTGCATAGAGCAATAAAGCTGTGCGAACCAGCTCTAGTTTCCAGTGATCCCACCGTAACACCACTTGGAACTTATCAGGAGGCTCATGTGTTCAAATCAAATTCTGGAGCTTGTGCCGCATTTCTTGCAAACTACAATCGGAAATCATTTGCAAAAGTGGCATTTGGGAACATGCATTACAACCTGCCTCCATGGTCTATTAGCATTCTTCCTGACTGCAAGAACACAGTTTATAACACTGCAAGGATTGGAGCGCAAACTGCACGAATGAAGATGCCCCGTGTTCCTATTCATGGAGGATTCTCTTGGCAAGCTTACAATGATGAAACTGCCACGTATAGTGACACTTCATTTACCACAGCCGGATTGTTGGAGCAGATAAATACCACTAGAGATGCCACTGATTACTTGTGGTACATGACAGATGTTAAGATTGATCCCAGTGAGGACTTTTTGCGAAGTGGAAATTATCCTGTGCTCACAGTCTTATCAGCAGGTCATGCTTTGCGTGTTTTCATCAATGGTCAACTTGCTGGAACTGCTTATGGAAGTCTAGAAACCCCAAAACTAACATTTAAGCAAGGTGTGAATTTGAGAGCTGGTATCAACCAAATTGCCCTTCTCAGCATTGCTGTTGGTCTGCCGAACGTTGGGCCACATTTTGAGACATGGAATGCTGGCATTCTTGGCCCAGTCATCTTAAATGGTCTTAATGAGGGAAGGAGGGACTTGTCATGGCAGAAATGGTCTTATAAGATTGGACTTAAAGGAGAAGCTTTGAGTCTTCATTCGCTGACTGGGAGTTCCTCAGTAGATTGGACTGAGGGGTCTTTTGTGGCACAAAGGCAGCCTCTTACATGGTACAAAACAACTTTCAATAGACCAGCTGGAAATTCACCATTGGCTTTAGATATGGGCAGCATGGGCAAAGGTCAAGTTTGGATAAATGGCCGGAGTATTGGCCGCTATTGGCCTGCTTATAAAGCATCTGGTACTTGTGGTGAATGCAACTATGCCGGAACATTTAGTGAGAAGAAATGCTTATGTAATTGTGGGGAGGCTTCTCAGAGATGGTATCATGTTCCTCGGTCATGGCTGAACCCAGCAGGCAACTTGTTGGTTGTGTTAGAAGAGTGGGGTGGTGACCCAAATGGGATATTTTTGGTCAGAAGGGAAGTAGATAGTGTATGTGCTGATATTTATGAGTGGCAACCAAATCTTATGAGTTGGCAAATGCAAGTCTCTGGAAGGGTCAATAAACCTCTAAGGCCTAAAGCTCATTTGTCATGCGGCCCTGGCCAGAAAATCTCATCAATAAAGTTTGCTAGCTTTGGAACACCAGAAGGGGTTTGTGGAAGCTTCCGAGAGGGAGGCTGTCATGCTCACAAGTCTTATAATGCTTTTGAAAGGAGTTGCATTGGACAGAACTCATGCTCAGTAACTGTATCACCTGAGAATTTTGGAGGGGATCCATGTCCAAATGTCATGAAGAAACTCTCTGTGGAGGCCATTTGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005911 GO:0009505 GO:0009506 GO:0015925 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

844

Amino Acids

93.45

Weight (kDa)

8.45

Isoelectric Point (pI)

36.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	37 - 341	5.9e-121	Glycosyl hydrolases family 35
GHD	PF17834	364 - 420	5.2e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	473 - 536	2.1e-06	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	622 - 708	1.8e-25	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13750
fragaria_vesca	FvH4_6g33260 FvH4_6g33260 FvH4_6g33260 FvH4_6g33260 FvH4_6g33260
malus_domestica	MD03G1276100.v1.1 MD05G1210900.v1.1 MD09G1193900.v1.1 MD11G1104200.v1.1 MD11G1105200.v1.1 MD15G1317000.v1.1 MD16G1228200.v1.1
prunus_persica	Prupe.3G050200_v2.0.a1
pyrus_communis	pycom09g11040 pycom111g03560 pycom15g12320
rosa_chinensis	RchiOBHm_Chr0c25g0500881 RchiOBHm_Chr1g0331081 RchiOBHm_Chr2g0142961 RchiOBHm_Chr6g0257481 RchiOBHm_Chr6g0266311
rosa_laevigata	RLG00000020004
rosa_multiflora	Rmu_sc0006428.1_g000002 Rmu_sc0008528.1_g000001 Rmu_sc0028483.1_g000003
rosa_roxburghii	Rroxscaffold_1G00030970 Rroxscaffold_2G00102180 Rroxscaffold_7G00183160 Rroxscaffold_7G00216290
rosa_rugosa	Rorug02G0377400
rosa_samantha	Rh2AG100700 Rh2AG430500 Rh2BG138700 Rh2BG439500 Rh2CG416200 Rh2DG449500 Rh4CG196100 Rh5DG216000 Rh6AG145200 Rh6AG372600 Rh6AG422200 Rh6DG373500
rosa_wichuraiana	Rw2G035160 Rw5G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	5 cut(s)	744, 1245, 1764, 1992, 2406
AasI	GACNNNNNNGTC	1 cut(s)	1837
Acc36I	ACCTGC	2 cut(s)	1203, 1496
AciI	CCGC	4 cut(s)	525, 1128, 1975, 2301
AclI	AACGTT	1 cut(s)	1659
AclWI	GGATC	9 cut(s)	127, 140, 154, 462, 677, 1052, 1442, 2474, 2487
AcoI	YGGCCR	5 cut(s)	121, 182, 1960, 1972, 2308
AcsI	RAATTY	6 cut(s)	305, 901, 1111, 1600, 1903, 2467
AcuI	CTGAAG	2 cut(s)	425, 783
AfaI	GTAC	5 cut(s)	321, 410, 1427, 1874, 2005
AfiI	CCNNNNNNNGG	5 cut(s)	266, 774, 995, 1304, 1661
AgsI	TTSAA	7 cut(s)	10, 451, 551, 1006, 1105, 1888, 2417
AhlI	ACTAGT	1 cut(s)	938
AjnI	CCWGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
AjuI	GAANNNNNNNTTGG	4 cut(s)	564, 596, 1614, 1646
AloI	GAACNNNNNNTCC	2 cut(s)	786, 818
Alw21I	GWGCWC	2 cut(s)	589, 1491
Alw26I	GTCTC	3 cut(s)	522, 1670, 2257
AlwI	GGATC	9 cut(s)	127, 140, 154, 462, 677, 1052, 1442, 2474, 2487
AlwNI	CAGNNNCTG	1 cut(s)	2453
ApeKI	GCWGC	4 cut(s)	38, 614, 1858, 1929
ApoI	RAATTY	6 cut(s)	305, 901, 1111, 1600, 1903, 2467
AspLEI	GCGC	1 cut(s)	1267
AspS9I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
AsuHPI	GGTGA	5 cut(s)	255, 1899, 2024, 2165, 2451
AsuNHI	GCTAGC	1 cut(s)	2336
AvaII	GGWCC	4 cut(s)	356, 443, 527, 923
BalI	TGGCCA	3 cut(s)	123, 184, 2310
BamHI	GGATCC	2 cut(s)	132, 2479
BbsI	GAAGAC	1 cut(s)	1787
Bbv12I	GWGCWC	2 cut(s)	589, 1491
BbvCI	CCTCAGC	1 cut(s)	819
BbvI	GCAGC	4 cut(s)	50, 626, 1870, 1941
BccI	CCATC	1 cut(s)	2076
BciT130I	CCWGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
BciVI	GTATCC	1 cut(s)	431
BcoDI	GTCTC	3 cut(s)	522, 1670, 2257
BcuI	ACTAGT	1 cut(s)	938
BfaI	CTAG	5 cut(s)	939, 1046, 1401, 1565, 2337
BfuAI	ACCTGC	2 cut(s)	1203, 1496
BfuI	GTATCC	1 cut(s)	431
BglII	AGATCT	2 cut(s)	187, 1009
BisI	GCNGC	7 cut(s)	39, 615, 1128, 1859, 1930, 1975, 2302
BlsI	GCNGC	7 cut(s)	40, 616, 1129, 1860, 1931, 1976, 2303
Bme1390I	CCNGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
Bme18I	GGWCC	4 cut(s)	356, 443, 527, 923
BmgT120I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
BmiI	GGNNCC	4 cut(s)	134, 565, 998, 2481
BmrFI	CCNGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
BmrI	ACTGGG	5 cut(s)	619, 783, 1446, 1697, 1818
BmsI	GCATC	5 cut(s)	664, 943, 1275, 1396, 2006
BmtI	GCTAGC	1 cut(s)	2340
BmuI	ACTGGG	5 cut(s)	619, 783, 1446, 1697, 1818
BoxI	GACNNNNGTC	1 cut(s)	644
BpiI	GAAGAC	1 cut(s)	1787
BpmI	CTGGAG	1 cut(s)	1137
Bpu10I	CCTNAGC	1 cut(s)	819
BsaAI	YACGTR	1 cut(s)	1347
BsaJI	CCNNGG	9 cut(s)	45, 102, 204, 567, 650, 651, 1202, 2096, 2305
BsaXI	ACNNNNNCTCC	2 cut(s)	786, 816
Bsc4I	CCNNNNNNNGG	5 cut(s)	266, 774, 995, 1304, 1661
Bse1I	ACTGG	8 cut(s)	395, 614, 646, 778, 1053, 1452, 1703, 1813
Bse3DI	GCAATG	2 cut(s)	709, 1638
BseBI	CCWGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
BseDI	CCNNGG	9 cut(s)	45, 102, 204, 567, 650, 651, 1202, 2096, 2305
BseGI	GGATG	2 cut(s)	226, 970
BseLI	CCNNNNNNNGG	5 cut(s)	266, 774, 995, 1304, 1661
BseMI	GCAATG	2 cut(s)	709, 1638
BseNI	ACTGG	8 cut(s)	395, 614, 646, 778, 1053, 1452, 1703, 1813
BseRI	GAGGAG	2 cut(s)	142, 948
BseXI	GCAGC	4 cut(s)	50, 626, 1870, 1941
BseYI	CCCAGC	1 cut(s)	2113
BsgI	GTGCAG	3 cut(s)	390, 819, 1257
BsiHKAI	GWGCWC	2 cut(s)	589, 1491
BsiSI	CCGG	3 cut(s)	1374, 1963, 2028
BslFI	GGGAC	3 cut(s)	632, 652, 1751
BslI	CCNNNNNNNGG	5 cut(s)	266, 774, 995, 1304, 1661
BsmAI	GTCTC	3 cut(s)	522, 1670, 2257
BsmFI	GGGAC	3 cut(s)	632, 652, 1751
BsmI	GAATGC	5 cut(s)	866, 1215, 1690, 1692, 2021
Bsp1286I	GDGCHC	2 cut(s)	589, 1491
Bsp19I	CCATGG	2 cut(s)	567, 1202
BspACI	CCGC	4 cut(s)	525, 1128, 1975, 2301
BspHI	TCATGA	1 cut(s)	2496
BspLI	GGNNCC	4 cut(s)	134, 565, 998, 2481
BspMI	ACCTGC	2 cut(s)	1203, 1496
BspOI	GCTAGC	1 cut(s)	2340
BspPI	GGATC	9 cut(s)	127, 140, 154, 462, 677, 1052, 1442, 2474, 2487
BsrDI	GCAATG	2 cut(s)	709, 1638
BsrI	ACTGG	8 cut(s)	395, 614, 646, 778, 1053, 1452, 1703, 1813
BssECI	CCNNGG	9 cut(s)	45, 102, 204, 567, 650, 651, 1202, 2096, 2305
BssT1I	CCWWGG	4 cut(s)	102, 204, 567, 1202
Bst2UI	CCWGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
Bst4CI	ACNGT	5 cut(s)	978, 1066, 1240, 1495, 2455
Bst6I	CTCTTC	1 cut(s)	2136
BstAPI	GCANNNNNTGC	2 cut(s)	1136, 1511
BstBAI	YACGTR	1 cut(s)	1347
BstC8I	GCNNGC	8 cut(s)	327, 331, 700, 1323, 1690, 1986, 2119, 2338
BstDSI	CCRYGG	2 cut(s)	567, 1202
BstEII	GGTNACC	1 cut(s)	2153
BstF5I	GGATG	2 cut(s)	226, 970
BstHHI	GCGC	1 cut(s)	1267
BstMAI	GTCTC	3 cut(s)	522, 1670, 2257
BstNI	CCWGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
BstNSI	RCATGY	1 cut(s)	1186
BstPAI	GACNNNNGTC	1 cut(s)	644
BstPI	GGTNACC	1 cut(s)	2153
BstSCI	CCNGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
BstV1I	GCAGC	4 cut(s)	50, 626, 1870, 1941
BstV2I	GAAGAC	1 cut(s)	1787
BstX2I	RGATCY	5 cut(s)	132, 146, 187, 1009, 2479
BstXI	CCANNNNNNTGG	1 cut(s)	756
BstYI	RGATCY	5 cut(s)	132, 146, 187, 1009, 2479
BsuI	GTATCC	1 cut(s)	431
BtgI	CCRYGG	2 cut(s)	567, 1202
BtgZI	GCGATG	1 cut(s)	916
BtsCI	GGATG	2 cut(s)	226, 970
BtsI	GCAGTG	1 cut(s)	1248
BtsIMutI	CAGTG	5 cut(s)	603, 1060, 1248, 1410, 1459
BveI	ACCTGC	2 cut(s)	1203, 1496
Cac8I	GCNNGC	8 cut(s)	327, 331, 700, 1323, 1690, 1986, 2119, 2338
CaiI	CAGNNNCTG	1 cut(s)	2453
CciI	TCATGA	1 cut(s)	2496
CfoI	GCGC	1 cut(s)	1267
Cfr13I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
CsiI	ACCWGGT	1 cut(s)	589
Csp6I	GTAC	5 cut(s)	320, 409, 1426, 1873, 2004
CspCI	CAANNNNNGTGG	2 cut(s)	30, 65
CviQI	GTAC	5 cut(s)	320, 409, 1426, 1873, 2004
DrdI	GACNNNNNNGTC	1 cut(s)	1837
DseDI	GACNNNNNNGTC	1 cut(s)	1837
EaeI	YGGCCR	5 cut(s)	121, 182, 1960, 1972, 2308
Eam1104I	CTCTTC	1 cut(s)	2136
EarI	CTCTTC	1 cut(s)	2136
Eco130I	CCWWGG	4 cut(s)	102, 204, 567, 1202
Eco147I	AGGCCT	2 cut(s)	333, 2281
Eco47I	GGWCC	4 cut(s)	356, 443, 527, 923
Eco57I	CTGAAG	2 cut(s)	425, 783
Eco91I	GGTNACC	1 cut(s)	2153
EcoO65I	GGTNACC	1 cut(s)	2153
EcoRII	CCWGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
EcoT14I	CCWWGG	4 cut(s)	102, 204, 567, 1202
EcoT22I	ATGCAT	1 cut(s)	1188
ErhI	CCWWGG	4 cut(s)	102, 204, 567, 1202
FalI	AAGNNNNNCTT	2 cut(s)	2036, 2068
FaqI	GGGAC	3 cut(s)	632, 652, 1751
Fnu4HI	GCNGC	7 cut(s)	39, 615, 1128, 1859, 1930, 1975, 2302
FokI	GGATG	2 cut(s)	233, 977
Fsp4HI	GCNGC	7 cut(s)	39, 615, 1128, 1859, 1930, 1975, 2302
FspBI	CTAG	5 cut(s)	939, 1046, 1401, 1565, 2337
GlaI	GCGC	1 cut(s)	1266
GluI	GCNGC	7 cut(s)	39, 615, 1128, 1859, 1930, 1975, 2302
GsaI	CCCAGC	1 cut(s)	2117
GsuI	CTGGAG	1 cut(s)	1137
HapII	CCGG	3 cut(s)	1374, 1963, 2028
HhaI	GCGC	1 cut(s)	1267
Hin6I	GCGC	1 cut(s)	1265
HinP1I	GCGC	1 cut(s)	1265
HincII	GTYRAC	1 cut(s)	1538
HindII	GTYRAC	1 cut(s)	1538
HindIII	AAGCTT	4 cut(s)	11, 1323, 1785, 2368
HinfI	GANTC	6 cut(s)	98, 226, 468, 515, 1311, 1792
HpaII	CCGG	3 cut(s)	1374, 1963, 2028
HphI	GGTGA	5 cut(s)	255, 1899, 2024, 2165, 2451
Hpy166II	GTNNAC	4 cut(s)	487, 759, 783, 1538
Hpy188I	TCNGA	7 cut(s)	198, 231, 431, 1152, 2079, 2180, 2375
Hpy8I	GTNNAC	4 cut(s)	487, 759, 783, 1538
HpyAV	CCTTC	6 cut(s)	270, 1640, 1725, 2175, 2253, 2348
HpyCH4III	ACNGT	5 cut(s)	978, 1066, 1240, 1495, 2455
HpyCH4IV	ACGT	2 cut(s)	1346, 1659
HpySE526I	ACGT	2 cut(s)	1346, 1659
HspAI	GCGC	1 cut(s)	1265
LmnI	GCTCC	6 cut(s)	584, 863, 961, 1118, 1262, 1384
Lsp1109I	GCAGC	4 cut(s)	50, 626, 1870, 1941
LweI	GCATC	5 cut(s)	664, 943, 1275, 1396, 2006
MabI	ACCWGGT	1 cut(s)	589
MaeI	CTAG	5 cut(s)	939, 1046, 1401, 1565, 2337
MaeII	ACGT	2 cut(s)	1346, 1659
MaeIII	GTNAC	4 cut(s)	1066, 1352, 2153, 2449
MboII	GAAGA	6 cut(s)	1211, 1294, 1787, 2056, 2153, 2512
MflI	RGATCY	5 cut(s)	132, 146, 187, 1009, 2479
MhlI	GDGCHC	2 cut(s)	589, 1491
MlsI	TGGCCA	3 cut(s)	123, 184, 2310
MluNI	TGGCCA	3 cut(s)	123, 184, 2310
MlyI	GAGTC	2 cut(s)	524, 1801
MmeI	TCCRAC	2 cut(s)	610, 1362
Mox20I	TGGCCA	3 cut(s)	123, 184, 2310
Mph1103I	ATGCAT	1 cut(s)	1188
MscI	TGGCCA	3 cut(s)	123, 184, 2310
MseI	TTAA	7 cut(s)	693, 873, 1440, 1587, 1712, 1722, 1776
Msp20I	TGGCCA	3 cut(s)	123, 184, 2310
MspA1I	CMGCKG	2 cut(s)	818, 1898
MspI	CCGG	3 cut(s)	1374, 1963, 2028
MspR9I	CCNGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
Mva1269I	GAATGC	5 cut(s)	866, 1215, 1690, 1692, 2021
MvaI	CCWGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
NcoI	CCATGG	2 cut(s)	567, 1202
NheI	GCTAGC	1 cut(s)	2336
NlaIV	GGNNCC	4 cut(s)	134, 565, 998, 2481
NmuCI	GTSAC	2 cut(s)	1352, 2153
NsiI	ATGCAT	1 cut(s)	1188
NspI	RCATGY	1 cut(s)	1186
PagI	TCATGA	1 cut(s)	2496
PasI	CCCWGGG	1 cut(s)	651
PceI	AGGCCT	2 cut(s)	333, 2281
PctI	GAATGC	5 cut(s)	866, 1215, 1690, 1692, 2021
PfeI	GAWTC	4 cut(s)	98, 226, 468, 1311
PkrI	GCNGC	7 cut(s)	40, 616, 1129, 1860, 1931, 1976, 2303
PleI	GAGTC	2 cut(s)	523, 1800
PpsI	GAGTC	2 cut(s)	523, 1800
Ppu21I	YACGTR	1 cut(s)	1347
PshAI	GACNNNNGTC	1 cut(s)	644
PsiI	TTATAA	5 cut(s)	744, 1245, 1764, 1992, 2406
Psp1406I	AACGTT	1 cut(s)	1659
Psp6I	CCWGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
PspEI	GGTNACC	1 cut(s)	2153
PspFI	CCCAGC	1 cut(s)	2113
PspGI	CCWGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
PspN4I	GGNNCC	4 cut(s)	134, 565, 998, 2481
PspPI	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
PsrI	GAACNNNNNNTAC	2 cut(s)	1172, 1204
PstNI	CAGNNNCTG	1 cut(s)	2453
PsuI	RGATCY	5 cut(s)	132, 146, 187, 1009, 2479
PvuII	CAGCTG	2 cut(s)	818, 1898
RsaI	GTAC	5 cut(s)	321, 410, 1427, 1874, 2005
RsaNI	GTAC	5 cut(s)	320, 409, 1426, 1873, 2004
SaqAI	TTAA	7 cut(s)	693, 873, 1440, 1587, 1712, 1722, 1776
SatI	GCNGC	7 cut(s)	39, 615, 1128, 1859, 1930, 1975, 2302
Sau96I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
SchI	GAGTC	2 cut(s)	524, 1801
ScrFI	CCNGG	7 cut(s)	47, 267, 417, 591, 652, 769, 2307
SduI	GDGCHC	2 cut(s)	589, 1491
SexAI	ACCWGGT	1 cut(s)	589
SfaNI	GCATC	5 cut(s)	664, 943, 1275, 1396, 2006
SinI	GGWCC	4 cut(s)	356, 443, 527, 923
SpeI	ACTAGT	1 cut(s)	938
SseBI	AGGCCT	2 cut(s)	333, 2281
SsiI	CCGC	4 cut(s)	525, 1128, 1975, 2301
SspMI	CTAG	5 cut(s)	939, 1046, 1401, 1565, 2337
StuI	AGGCCT	2 cut(s)	333, 2281
StyD4I	CCNGG	7 cut(s)	45, 265, 415, 589, 650, 767, 2305
StyI	CCWWGG	4 cut(s)	102, 204, 567, 1202
TaaI	ACNGT	5 cut(s)	978, 1066, 1240, 1495, 2455
TaiI	ACGT	2 cut(s)	1349, 1662
TaqI	TCGA	1 cut(s)	513
TaqII	GACCGA	1 cut(s)	2088
TatI	WGTACW	1 cut(s)	408
TauI	GCSGC	3 cut(s)	1130, 1977, 2304
TfiI	GAWTC	4 cut(s)	98, 226, 468, 1311
Tru1I	TTAA	7 cut(s)	693, 873, 1440, 1587, 1712, 1722, 1776
Tru9I	TTAA	7 cut(s)	693, 873, 1440, 1587, 1712, 1722, 1776
TscAI	CASTG	5 cut(s)	610, 1060, 1255, 1417, 1459
TseFI	GTSAC	2 cut(s)	1352, 2153
TseI	GCWGC	4 cut(s)	38, 614, 1858, 1929
Tsp45I	GTSAC	2 cut(s)	1352, 2153
TspGWI	ACGGA	2 cut(s)	225, 987
TspRI	CASTG	5 cut(s)	610, 1060, 1255, 1417, 1459
VpaK11BI	GGWCC	4 cut(s)	356, 443, 527, 923
XapI	RAATTY	6 cut(s)	305, 901, 1111, 1600, 1903, 2467
XbaI	TCTAGA	1 cut(s)	1564
XceI	RCATGY	1 cut(s)	1186
XspI	CTAG	5 cut(s)	939, 1046, 1401, 1565, 2337
Zsp2I	ATGCAT	1 cut(s)	1188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.