MD03G1276100.v1.1

Beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001950
Physical Location & Seq
Reverse (-)
35862135 .. 35866330
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1276100.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGTCAACCATGGGAGTTTTGTTGCTGGCTTCTGCTCTCCTGTTGCTAATTCAGTTTTGCTCTGCTCTCCGGGTTTCATATGATGGAAATGCCCTAAAAATTGATGATGAAAGAAGAATTGTAATATCTGGCTCCATTCACTACCCCCGCAGTACTCCTGAAATGTGGCCATCCTTAATCCAAAAAGCAAAACACGGAGGTCTCAATGCCATTGAGACTTACGTGTTTTGGAATGCGCATGAACCTCTTCGTCGTCAGCATGATTTCACCGAAAATCTGGACCTAGTAAGATTCATTAAGGCCATCCAAGAGACGGGGCTCTATGCTGTTCTTCGAATTGGACCATATGTTTGTGCAGAATGGAATTATGGAGGTTTGCCTGTGTGGCTGCATAACATACCTGGCATCAAATTGAGGACAAACAATCCTGTGTTTGAGAGGGAGATGGCAAACTTTACAACCCTAATAGTTGATATGATGAAGCAGGAGAAACTTTTTGCTTCGCAAGGGGGACCTATTGTAGTTGCTCAGATTGAAAATGAGTACGGGAATGTTATTCGGTACTATGGGGATGAAGGAAAGCAATATTTACAATGGTGTGTGGGGTTGGCTGAATCTTTCAAGATTGAAGTGCCCTGGATTATGTGTCAAGAGAATGATGCTCCACAACCAATGATTGATACCTGCAATGGCTGGTATTGTGATCAATTTCAACCTGCCCACAACAATAGTCCCATGTGGACAGAAAATTGGACTGGCTGGTACAAGGGTTGGGGCATGCAAGATCCACACAGGACTGCCGTTGCGTGCTCTGTTGCACGTTTCTTTCAGCTTGGTGGAACCTTCCAAAACTATTACATGTGCCATGGTGGTACGAATTTCGGTCGTGTAGCAGGCGGTCCTTACATCACCACATCATATGATTATGATGCAACACTGGATGAATATGGTAATTTGAATGAACCCAAGTGGGGACATCTTCAAAAGCTCCATAATATCATCCTATCAAGGGAGAGGGCACTGACTTATGGCGCTGTAGATAAAATCGATTATGGAAACATGATGGAGGTACAGTTCCCTCTTGGGTCTGTCTCTGTCCTTCCTAATTGCTCTCATACTGAAGTCTACAACACTGCCCAGGGTAATACCCAAATATCAGTAATGGAGAGAGTGCTAAATGGAGCGGATGATAGTAAGGAGCCTTACGCATTAAACTGGGTTTGGAAGCCTGAGCACTTTACACACATCAAGAACGGCTCGGTTAAGCATAGCAATCTGACTGCTAATGAACTGCTTGATCAGAAATTAGTAACAAATGACACAAGTGATTACTTGTGGTATATGATTAGTCTGGACCACATTTCAACTGATCCAATTTGGGGTAACAAAGAAATTGCGCTACGAGTGAATACCAATGGGCACATCCTTCATGCTTTTGTTAATGGAAAACACATGGGTAAAAGAGCTGCTAAAAATGGACAGTTCAGCTTCACTTTTGAGACAAAGTCGTTCAACCTAAAGCACGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

510

Amino Acids

57.95

Weight (kDa)

6.17

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 32 - 332 4.1e-110 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 237
Acc36I ACCTGC 2 cut(s) 692, 724
AccBSI CCGCTC 1 cut(s) 1184
AccI GTMKAC 1 cut(s) 1125
AciI CCGC 3 cut(s) 148, 897, 1184
AclWI GGATC 2 cut(s) 779, 1364
AcoI YGGCCR 1 cut(s) 167
AcsI RAATTY 1 cut(s) 877
AcuI CTGAAG 1 cut(s) 1140
AfaI GTAC 6 cut(s) 154, 545, 563, 764, 874, 1071
AfiI CCNNNNNNNGG 4 cut(s) 313, 971, 1009, 1379
AflIII ACRYGT 2 cut(s) 222, 858
AgsI TTSAA 8 cut(s) 536, 622, 629, 713, 958, 983, 1365, 1513
AjnI CCWGG 3 cut(s) 400, 635, 1137
AloI GAACNNNNNNTCC 2 cut(s) 1058, 1090
AluBI AGCT 4 cut(s) 832, 988, 1466, 1488
AluI AGCT 4 cut(s) 832, 988, 1466, 1488
Alw21I GWGCWC 2 cut(s) 812, 1236
Alw26I GTCTC 5 cut(s) 206, 209, 305, 1096, 1493
AlwI GGATC 2 cut(s) 779, 1364
AoxI GGCC 2 cut(s) 167, 300
ApeKI GCWGC 2 cut(s) 388, 1466
ApoI RAATTY 1 cut(s) 877
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
Asp700I GAANNNNTTC 1 cut(s) 246
AspLEI GCGC 3 cut(s) 238, 1034, 1399
AspS9I GGNCC 5 cut(s) 280, 341, 512, 899, 1354
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 259, 901
AsuII TTCGAA 1 cut(s) 334
AvaII GGWCC 5 cut(s) 280, 341, 512, 899, 1354
BaeGI GKGCMC 3 cut(s) 636, 1021, 1422
BaeI ACNNNNGTAYC 2 cut(s) 754, 787
BalI TGGCCA 1 cut(s) 169
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 812, 1236
BbvI GCAGC 2 cut(s) 375, 1453
BccI CCATC 5 cut(s) 77, 178, 311, 439, 1057
BceAI ACGGC 2 cut(s) 785, 1270
BciT130I CCWGG 3 cut(s) 402, 637, 1139
BclI TGATCA 2 cut(s) 703, 1297
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 5 cut(s) 206, 209, 305, 1096, 1493
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 1035
BfoI RGCGCY 1 cut(s) 1035
BfuAI ACCTGC 2 cut(s) 692, 724
BglI GCCNNNNNGGC 1 cut(s) 385
BisI GCNGC 2 cut(s) 389, 1467
BlsI GCNGC 2 cut(s) 390, 1468
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 4 cut(s) 71, 402, 637, 1139
Bme18I GGWCC 5 cut(s) 280, 341, 512, 899, 1354
BmgT120I GGNCC 5 cut(s) 280, 341, 512, 899, 1354
BmiI GGNNCC 4 cut(s) 133, 513, 841, 1200
BmrFI CCNGG 4 cut(s) 71, 402, 637, 1139
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 3 cut(s) 414, 649, 919
BmuI ACTGGG 1 cut(s) 1225
Bpu10I CCTNAGC 1 cut(s) 1230
Bpu14I TTCGAA 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 1047
BsaAI YACGTR 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 5 cut(s) 9, 635, 865, 1137, 1138
BsaXI ACNNNNNCTCC 2 cut(s) 1058, 1088
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 971, 1009, 1379
Bse1I ACTGG 3 cut(s) 760, 942, 1220
Bse3DI GCAATG 1 cut(s) 694
BseBI CCWGG 3 cut(s) 402, 637, 1139
BseCI ATCGAT 1 cut(s) 1047
BseDI CCNNGG 5 cut(s) 9, 635, 865, 1137, 1138
BseGI GGATG 7 cut(s) 170, 303, 577, 946, 999, 1192, 1422
BseLI CCNNNNNNNGG 4 cut(s) 313, 971, 1009, 1379
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 2 cut(s) 542, 1221
BseNI ACTGG 3 cut(s) 760, 942, 1220
BseSI GKGCMC 3 cut(s) 636, 1021, 1422
BseXI GCAGC 2 cut(s) 375, 1453
BsgI GTGCAG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 886
BshFI GGCC 2 cut(s) 169, 302
BshVI ATCGAT 1 cut(s) 1047
BsiEI CGRYCG 1 cut(s) 886
BsiHKAI GWGCWC 2 cut(s) 812, 1236
BsiSI CCGG 1 cut(s) 70
BslFI GGGAC 3 cut(s) 525, 717, 987
BslI CCNNNNNNNGG 4 cut(s) 313, 971, 1009, 1379
BsmAI GTCTC 5 cut(s) 206, 209, 305, 1096, 1493
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 3 cut(s) 525, 717, 987
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 169, 302
Bso31I GGTCTC 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 334
Bsp1286I GDGCHC 6 cut(s) 321, 636, 812, 1021, 1236, 1422
Bsp143I GATC 4 cut(s) 703, 784, 1297, 1369
Bsp19I CCATGG 2 cut(s) 9, 865
BspACI CCGC 3 cut(s) 148, 897, 1184
BspANI GGCC 2 cut(s) 169, 302
BspCNI CTCAG 2 cut(s) 541, 1222
BspDI ATCGAT 1 cut(s) 1047
BspLI GGNNCC 4 cut(s) 133, 513, 841, 1200
BspMI ACCTGC 2 cut(s) 692, 724
BspPI GGATC 2 cut(s) 779, 1364
BspT104I TTCGAA 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 1184
BsrDI GCAATG 1 cut(s) 694
BsrI ACTGG 3 cut(s) 760, 942, 1220
BssECI CCNNGG 5 cut(s) 9, 635, 865, 1137, 1138
BssMI GATC 4 cut(s) 703, 784, 1297, 1369
BssT1I CCWWGG 2 cut(s) 9, 865
Bst2UI CCWGG 3 cut(s) 402, 637, 1139
Bst4CI ACNGT 2 cut(s) 1074, 1482
Bst6I CTCTTC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 334
BstC8I GCNNGC 4 cut(s) 27, 779, 808, 895
BstDEI CTNAG 2 cut(s) 528, 1230
BstDSI CCRYGG 2 cut(s) 9, 865
BstF5I GGATG 7 cut(s) 170, 303, 577, 946, 999, 1192, 1422
BstH2I RGCGCY 1 cut(s) 1035
BstHHI GCGC 3 cut(s) 238, 1034, 1399
BstKTI GATC 4 cut(s) 706, 787, 1300, 1372
BstMAI GTCTC 5 cut(s) 206, 209, 305, 1096, 1493
BstMBI GATC 4 cut(s) 703, 784, 1297, 1369
BstMCI CGRYCG 1 cut(s) 886
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNI CCWGG 3 cut(s) 402, 637, 1139
BstNSI RCATGY 2 cut(s) 781, 862
BstSCI CCNGG 4 cut(s) 69, 400, 635, 1137
BstSFI CTRYAG 1 cut(s) 1035
BstSLI GKGCMC 3 cut(s) 636, 1021, 1422
BstV1I GCAGC 2 cut(s) 375, 1453
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 1047
BsuRI GGCC 2 cut(s) 169, 302
BsuTUI ATCGAT 1 cut(s) 1047
BtgI CCRYGG 2 cut(s) 9, 865
BtsCI GGATG 7 cut(s) 170, 303, 577, 946, 999, 1192, 1422
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 3 cut(s) 935, 1019, 1131
BveI ACCTGC 2 cut(s) 692, 724
Cac8I GCNNGC 4 cut(s) 27, 779, 808, 895
CfoI GCGC 3 cut(s) 238, 1034, 1399
Cfr13I GGNCC 5 cut(s) 280, 341, 512, 899, 1354
ClaI ATCGAT 1 cut(s) 1047
Csp6I GTAC 6 cut(s) 153, 544, 562, 763, 873, 1070
CviQI GTAC 6 cut(s) 153, 544, 562, 763, 873, 1070
DdeI CTNAG 2 cut(s) 528, 1230
DpnI GATC 4 cut(s) 705, 786, 1299, 1371
DpnII GATC 4 cut(s) 703, 784, 1297, 1369
EaeI YGGCCR 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 9, 865
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 5 cut(s) 280, 341, 512, 899, 1354
Eco57I CTGAAG 1 cut(s) 1140
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 3 cut(s) 400, 635, 1137
EcoT14I CCWWGG 2 cut(s) 9, 865
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 9, 865
Esp3I CGTCTC 1 cut(s) 305
FaqI GGGAC 3 cut(s) 525, 717, 987
FauI CCCGC 1 cut(s) 155
FauNDI CATATG 3 cut(s) 79, 346, 919
FbaI TGATCA 2 cut(s) 703, 1297
FblI GTMKAC 1 cut(s) 1125
Fnu4HI GCNGC 2 cut(s) 389, 1467
FokI GGATG 7 cut(s) 157, 290, 584, 953, 986, 1199, 1409
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 2 cut(s) 389, 1467
FspAI RTGCGCAY 1 cut(s) 237
FspBI CTAG 1 cut(s) 284
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 3 cut(s) 237, 1033, 1398
GluI GCNGC 2 cut(s) 389, 1467
HaeII RGCGCY 1 cut(s) 1035
HaeIII GGCC 2 cut(s) 169, 302
HapII CCGG 1 cut(s) 70
HhaI GCGC 3 cut(s) 238, 1034, 1399
Hin6I GCGC 3 cut(s) 236, 1032, 1397
HinP1I GCGC 3 cut(s) 236, 1032, 1397
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 291, 614
HpaII CCGG 1 cut(s) 70
HphI GGTGA 2 cut(s) 259, 901
Hpy166II GTNNAC 3 cut(s) 6, 741, 1126
Hpy188I TCNGA 3 cut(s) 531, 1278, 1302
Hpy188III TCNNGA 6 cut(s) 158, 278, 622, 650, 1249, 1352
Hpy8I GTNNAC 3 cut(s) 6, 741, 1126
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 4 cut(s) 569, 853, 1109, 1436
HpyCH4III ACNGT 2 cut(s) 1074, 1482
HpyCH4IV ACGT 2 cut(s) 222, 820
HpyCH4V TGCA 6 cut(s) 356, 391, 687, 781, 818, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 2 cut(s) 528, 1230
HpySE526I ACGT 2 cut(s) 222, 820
HspAI GCGC 3 cut(s) 236, 1032, 1397
Ksp22I TGATCA 2 cut(s) 703, 1297
Kzo9I GATC 4 cut(s) 703, 784, 1297, 1369
LmnI GCTCC 5 cut(s) 137, 667, 993, 1181, 1198
Lsp1109I GCAGC 2 cut(s) 375, 1453
LweI GCATC 3 cut(s) 414, 649, 919
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 222, 820
MaeIII GTNAC 2 cut(s) 1309, 1382
MalI GATC 4 cut(s) 705, 786, 1299, 1371
MbiI CCGCTC 1 cut(s) 1184
MboI GATC 4 cut(s) 703, 784, 1297, 1369
MboII GAAGA 4 cut(s) 126, 239, 323, 971
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 6 cut(s) 321, 636, 812, 1021, 1236, 1422
MlsI TGGCCA 1 cut(s) 169
MluNI TGGCCA 1 cut(s) 169
MnlI CCTC 8 cut(s) 191, 255, 365, 408, 432, 1008, 1060, 1089
Mox20I TGGCCA 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 246
MscI TGGCCA 1 cut(s) 169
MseI TTAA 5 cut(s) 176, 297, 1211, 1263, 1440
Msp20I TGGCCA 1 cut(s) 169
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 4 cut(s) 71, 402, 637, 1139
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 3 cut(s) 402, 637, 1139
MwoI GCNNNNNNNGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 71
NcoI CCATGG 2 cut(s) 9, 865
NdeI CATATG 3 cut(s) 79, 346, 919
NdeII GATC 4 cut(s) 703, 784, 1297, 1369
NlaIV GGNNCC 4 cut(s) 133, 513, 841, 1200
NsbI TGCGCA 1 cut(s) 237
NspI RCATGY 2 cut(s) 781, 862
NspV TTCGAA 1 cut(s) 334
PaeI GCATGC 1 cut(s) 781
PasI CCCWGGG 1 cut(s) 1138
PciI ACATGT 1 cut(s) 858
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 291, 614
PflFI GACNNNGTC 1 cut(s) 1504
PkrI GCNGC 2 cut(s) 390, 1468
Ppu21I YACGTR 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 512
PscI ACATGT 1 cut(s) 858
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 400, 635, 1137
PspGI CCWGG 3 cut(s) 400, 635, 1137
PspN4I GGNNCC 4 cut(s) 133, 513, 841, 1200
PspPI GGNCC 5 cut(s) 280, 341, 512, 899, 1354
PspPPI RGGWCCY 1 cut(s) 512
PsuI RGATCY 1 cut(s) 784
PsyI GACNNNGTC 1 cut(s) 1504
RsaI GTAC 6 cut(s) 154, 545, 563, 764, 874, 1071
RsaNI GTAC 6 cut(s) 153, 544, 562, 763, 873, 1070
SaqAI TTAA 5 cut(s) 176, 297, 1211, 1263, 1440
SatI GCNGC 2 cut(s) 389, 1467
Sau3AI GATC 4 cut(s) 703, 784, 1297, 1369
Sau96I GGNCC 5 cut(s) 280, 341, 512, 899, 1354
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 4 cut(s) 71, 402, 637, 1139
SduI GDGCHC 6 cut(s) 321, 636, 812, 1021, 1236, 1422
SfaNI GCATC 3 cut(s) 414, 649, 919
SfcI CTRYAG 1 cut(s) 1035
SfuI TTCGAA 1 cut(s) 334
SinI GGWCC 5 cut(s) 280, 341, 512, 899, 1354
SphI GCATGC 1 cut(s) 781
SsiI CCGC 3 cut(s) 148, 897, 1184
SspI AATATT 1 cut(s) 587
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 4 cut(s) 69, 400, 635, 1137
StyI CCWWGG 2 cut(s) 9, 865
TaaI ACNGT 2 cut(s) 1074, 1482
TaiI ACGT 2 cut(s) 225, 823
TaqI TCGA 2 cut(s) 334, 1047
TaqII GACCGA 1 cut(s) 872
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 2 cut(s) 291, 614
Tru1I TTAA 5 cut(s) 176, 297, 1211, 1263, 1440
Tru9I TTAA 5 cut(s) 176, 297, 1211, 1263, 1440
TscAI CASTG 3 cut(s) 942, 1026, 1138
TseI GCWGC 2 cut(s) 388, 1466
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 3 cut(s) 942, 1026, 1138
Tth111I GACNNNGTC 1 cut(s) 1504
VpaK11BI GGWCC 5 cut(s) 280, 341, 512, 899, 1354
XapI RAATTY 1 cut(s) 877
XceI RCATGY 2 cut(s) 781, 862
XmiI GTMKAC 1 cut(s) 1125
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.