Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001950

Physical Location & Seq

Genomic Coordinates Reverse (-)

17371011 .. 17377474

Gene Structure 6464 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1193900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGTTTTTTGAGGCTTGTAATGTGGAATGTGGCTGTGCTGCTGCTCGTGTTGCTGGGTTCATGGGTTGGTTCTGTGAGAGCTTCTGTTTCATATGATTCCAAGGCCATTGTGATTAATGGACACAGAAAGATCCTCATTTCTGGATCTATTCACTACCCAAGAAGTTCCCCTGAGATGTGGCCAGATCTTATTCAGAAAGCAAAGGAAGGAGGTCTGGATGTGATTCAGACTTATGTTTTCTGGAATGGGCATGAACCTTCACCTGGCAAATATTATTTTGAAGACAACTATGATTTGGTCAAGTTTATCAAGCTGGTTCAGCAGGCAGGCCTATATGTTCATCTCAGGATTGGTCCTTATGTTTGTGCTGAGTGGAACTTTGGGGGGTTTCCTGTTTGGCTGAAGTACATTCCTGGTATCCAATTCAGAACAGATAATGGTCCTTTCAAGAATCAAATGCAAAGATTCACAACCAAGATTGTAAATATGATGAAAGCAGAAAGGTTGTTCCAGACTCACGGTGGTCCTATAATTATGTCCCAGATTGAAAATGAGTATGGACCGATGGAGTATGAACTCGGTGCACCCGGTAAACAGTACACTGACTGGGCAGCTCAGATGGCTTTGGGGCTCGGCACCGGTGTCCCCTGGGTGATGTGCAAACAAGATGATGCTCCTGATCCTATTATTAATGCCTGCAATGGCTTCTACTGTGACTACTTCTCTCCAAACAAGGCATATAAACCAAAGATGTGGACAGAAGCCTGGACTGGCTGGTATACTGAGTTTGGAGGTGCAGTTCCTTACCGACCGGCTGAGGATTTAGCTTTCTCAGTTGCAAGGTTTATTCAGAAGGGTGGATCATTCATTAATTATTATATGTACCATGGAGGAACAAATTTTGGCCGAACTGCTGGAGGTCCTTTCATTGCTACTAGTTATGATTATGATGCTCCTCTTGATGAATACGGTCTATTGAGGCAACCTAAATGGGGCCATCTGAAAGATCTGCACAGAGCAATAAAACTGTGTGAACCAGCTCTAGTTTCTGCGGATCCCACTGTACAACCACTTGGCAGATTTCAAGAGGCTCATGTTTTCAAATCAAATTCTGGAGCTTGTTCTGCATTTCTTGCAAATTACAACCCAAGATCATTTGCGAAAGTGGCATTTGGAAATATGCATTACAACCTACCTCCTTGGTCTATTAGCATTCTTCCCGACTGCAAGAACACTGTTTATAACACCGCAAGAGTTGGTGCGCAGAGTGCACTAATGAAGATGCCTCGTGTTCCTCTTCACGGAGGATTCTCTTGGCAGTCGTACCCTGATGAGGCTGCCTCTTACAGTGACACTACATTTACCACAGCCGGGTTGTTGGAGCAGATAAATACCACTAGAGATTCCTCTGATTATCTGTGGTACATAACAGATGTTAATATCGATCCCAATGAGGAATTTTTGAGAAGCGGAAAGTATCCTGAACTCACCGTCTTATCAGCTGGCCATGCTTTGCGTGTTTTCATCAACGGTCAACTTGCAGGGACTTCTTACGGAAGTCTAGAATTCCCAAAACTAACATTTAGTCAAGGTGTGAATTTGAGAGCTGGTATCAACCAGATTGCGCTTCTAAGCATTGCTGTTGGCCTCCCGAATGTTGGTCCACATTTTGAGACTTGGAATGCTGGTGTTCTTGGCCCGGTCATATTAAATGGTCTGAATGAGGGAAGGAGGGACTTGTCATGGCAGAAATGGTCTTACAAGGTTGGGCTTAAAGGAGAAGCCCTGAGTCTTCATTCGCTCAGTGGGAGTTCCTCAGTCGAGTGGATTCAGGGGTCATATGTCACAAGAAAGCAGCCTCTGACATGGTTCAAAACAACCTTCAATGCACCAGCTGGAAATTCACCGTTGGCTCTAGATATGGGCAGCATGGGCAAAGGTCAAGTATGGATAAATGGCAGAAGTATTGGCCGCTACTGGCCTGCATATACAGCATCTGGTAATTGTGGCGCCTGTAACTATGCTGGGACATACAATGAGAAAAAATGCTTAAGTAACTGTGGACAGGCTTCCCAGAGATGGTATCATGTTCCTCGGTCGTGGCTAAACCCAACAGGCAATTTGTTGGTTGTGTTAGAAGAGTGGGGAGGAGATCCGAATGGGATATTTTTGGTGAGAAGGGAAGTAGATAGCATATGTGCTGATATTTACGAGTGGCAACCAAATCTTATGAGTTATCAAATGCAAGCCTCAGGCAAGGTGAAGAAACCGGTAAGGCCTAAAGCCCATTTGACATGTGGCCCTGGACAGAAAATATCATCAATAAAGTTTGCTAGTTTTGGAACGCCGGAAGGCGCTTGTGGAAGCTTCCGTGAGGGAGCCTGTCATGCCCACAACTCGTATGATGCTTTTCAAAGGAGTTGTATCGGACAGAACTCATGCTCGGTAACTGTAGAACCTGAGAATTTTGGAGGCGATCCATGTCCAAGTGTAATGAAGAAACTCTCCGTGGAGGCCATTTGCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005911 GO:0009505 GO:0009506 GO:0015925 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

844

Amino Acids

93.53

Weight (kDa)

8.5

Isoelectric Point (pI)

37.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	37 - 341	4.2e-121	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	58 - 189	9.8e-06	Beta-galactosidase
GHD	PF17834	364 - 420	9.1e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	622 - 708	1.5e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	765 - 842	1.7e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001950)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13750
fragaria_vesca	FvH4_6g33260 FvH4_6g33260 FvH4_6g33260 FvH4_6g33260 FvH4_6g33260
malus_domestica	MD03G1276100.v1.1 MD05G1210900.v1.1 MD09G1193900.v1.1 MD11G1104200.v1.1 MD11G1105200.v1.1 MD15G1317000.v1.1 MD16G1228200.v1.1
prunus_persica	Prupe.3G050200_v2.0.a1
pyrus_communis	pycom09g11040 pycom111g03560 pycom15g12320
rosa_chinensis	RchiOBHm_Chr0c25g0500881 RchiOBHm_Chr1g0331081 RchiOBHm_Chr2g0142961 RchiOBHm_Chr6g0257481 RchiOBHm_Chr6g0266311
rosa_laevigata	RLG00000020004
rosa_multiflora	Rmu_sc0006428.1_g000002 Rmu_sc0008528.1_g000001 Rmu_sc0028483.1_g000003
rosa_roxburghii	Rroxscaffold_1G00030970 Rroxscaffold_2G00102180 Rroxscaffold_7G00183160 Rroxscaffold_7G00216290
rosa_rugosa	Rorug02G0377400
rosa_samantha	Rh2AG100700 Rh2AG430500 Rh2BG138700 Rh2BG439500 Rh2CG416200 Rh2DG449500 Rh4CG196100 Rh5DG216000 Rh6AG145200 Rh6AG372600 Rh6AG422200 Rh6DG373500
rosa_wichuraiana	Rw2G035160 Rw5G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1245
Acc16I	TGCGCA	1 cut(s)	1266
AccB1I	GGYRCC	2 cut(s)	638, 2012
AccI	GTMKAC	1 cut(s)	782
AciI	CCGC	4 cut(s)	1055, 1251, 1473, 1975
AclWI	GGATC	9 cut(s)	127, 154, 677, 871, 1052, 1065, 1442, 2150, 2474
AcoI	YGGCCR	4 cut(s)	182, 907, 1507, 1972
AcsI	RAATTY	7 cut(s)	901, 1111, 1460, 1568, 1600, 1903, 2467
AcuI	CTGAAG	1 cut(s)	425
AcyI	GRCGYC	1 cut(s)	2013
AfaI	GTAC	6 cut(s)	410, 602, 887, 1068, 1328, 1427
AfiI	CCNNNNNNNGG	7 cut(s)	266, 995, 1304, 1336, 1374, 1661, 2082
AflII	CTTAAG	1 cut(s)	2053
AflIII	ACRYGT	1 cut(s)	2297
AgeI	ACCGGT	2 cut(s)	641, 2272
AgsI	TTSAA	8 cut(s)	284, 451, 551, 1088, 1105, 1876, 1888, 2417
AhlI	ACTAGT	1 cut(s)	938
AjnI	CCWGG	5 cut(s)	265, 415, 650, 767, 2305
AloI	GAACNNNNNNTCC	2 cut(s)	786, 818
Alw21I	GWGCWC	2 cut(s)	589, 1276
Alw26I	GTCTC	1 cut(s)	1670
Alw44I	GTGCAC	2 cut(s)	585, 1272
AlwI	GGATC	9 cut(s)	127, 154, 677, 871, 1052, 1065, 1442, 2150, 2474
AlwNI	CAGNNNCTG	2 cut(s)	1864, 2000
ApaLI	GTGCAC	2 cut(s)	585, 1272
ApeKI	GCWGC	7 cut(s)	40, 43, 614, 1340, 1858, 1929, 2526
ApoI	RAATTY	7 cut(s)	901, 1111, 1460, 1568, 1600, 1903, 2467
AseI	ATTAAT	3 cut(s)	117, 693, 873
AsiGI	ACCGGT	2 cut(s)	641, 2272
AspLEI	GCGC	4 cut(s)	1267, 1630, 2015, 2360
AspS9I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
AsuC2I	CCSGG	3 cut(s)	591, 1375, 1703
AsuHPI	GGTGA	6 cut(s)	255, 667, 1483, 1899, 2188, 2275
AvaII	GGWCC	6 cut(s)	356, 443, 527, 563, 923, 1664
AxyI	CCTNAGG	1 cut(s)	2254
BaeGI	GKGCMC	2 cut(s)	589, 1276
BalI	TGGCCA	2 cut(s)	184, 1509
BamHI	GGATCC	1 cut(s)	1057
BanI	GGYRCC	2 cut(s)	638, 2012
BanII	GRGCYC	1 cut(s)	636
BauI	CACGAG	2 cut(s)	47, 1290
BbsI	GAAGAC	2 cut(s)	291, 1787
Bbv12I	GWGCWC	2 cut(s)	589, 1276
BbvCI	CCTCAGC	1 cut(s)	819
BbvI	GCAGC	6 cut(s)	27, 30, 626, 1327, 1870, 1941
BccI	CCATC	4 cut(s)	562, 616, 1008, 2076
BciT130I	CCWGG	5 cut(s)	267, 417, 652, 769, 2307
BciVI	GTATCC	2 cut(s)	431, 1491
BcnI	CCSGG	3 cut(s)	591, 1375, 1703
BcoDI	GTCTC	1 cut(s)	1670
BcuI	ACTAGT	1 cut(s)	938
BfaI	CTAG	6 cut(s)	939, 1046, 1401, 1565, 1919, 2337
BfmI	CTRYAG	1 cut(s)	2454
BfoI	RGCGCY	2 cut(s)	2016, 2361
BfrI	CTTAAG	1 cut(s)	2053
BfuI	GTATCC	2 cut(s)	431, 1491
BglII	AGATCT	2 cut(s)	187, 1009
BisI	GCNGC	8 cut(s)	41, 44, 615, 1341, 1859, 1930, 1975, 2527
BlsI	GCNGC	8 cut(s)	42, 45, 616, 1342, 1860, 1931, 1976, 2528
Bme1390I	CCNGG	8 cut(s)	267, 417, 591, 652, 769, 1375, 1703, 2307
Bme18I	GGWCC	6 cut(s)	356, 443, 527, 563, 923, 1664
BmgT120I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
BmiI	GGNNCC	5 cut(s)	640, 998, 1059, 2014, 2383
BmrFI	CCNGG	8 cut(s)	267, 417, 591, 652, 769, 1375, 1703, 2307
BmrI	ACTGGG	1 cut(s)	619
BmsI	GCATC	5 cut(s)	664, 943, 1275, 2006, 2398
BmuI	ACTGGG	1 cut(s)	619
BpiI	GAAGAC	2 cut(s)	291, 1787
BplI	GAGNNNNNCTC	2 cut(s)	1328, 1360
BpmI	CTGGAG	2 cut(s)	939, 1137
Bpu10I	CCTNAGC	1 cut(s)	819
BpuMI	CCSGG	3 cut(s)	591, 1375, 1703
Bsa29I	ATCGAT	1 cut(s)	1446
BsaHI	GRCGYC	1 cut(s)	2013
BsaJI	CCNNGG	8 cut(s)	102, 650, 651, 889, 1202, 2096, 2305, 2511
BsaWI	WCCGGW	2 cut(s)	641, 2272
BsaXI	ACNNNNNCTCC	2 cut(s)	786, 816
Bsc4I	CCNNNNNNNGG	7 cut(s)	266, 995, 1304, 1336, 1374, 1661, 2082
Bse118I	RCCGGY	3 cut(s)	641, 814, 2272
Bse1I	ACTGG	3 cut(s)	614, 778, 1985
Bse21I	CCTNAGG	1 cut(s)	2254
Bse3DI	GCAATG	3 cut(s)	709, 930, 1638
BseBI	CCWGG	5 cut(s)	267, 417, 652, 769, 2307
BseCI	ATCGAT	1 cut(s)	1446
BseDI	CCNNGG	8 cut(s)	102, 650, 651, 889, 1202, 2096, 2305, 2511
BseGI	GGATG	1 cut(s)	226
BseLI	CCNNNNNNNGG	7 cut(s)	266, 995, 1304, 1336, 1374, 1661, 2082
BseMI	GCAATG	3 cut(s)	709, 930, 1638
BseNI	ACTGG	3 cut(s)	614, 778, 1985
BseRI	GAGGAG	2 cut(s)	948, 2166
BseSI	GKGCMC	2 cut(s)	589, 1276
BseXI	GCAGC	6 cut(s)	27, 30, 626, 1327, 1870, 1941
BseYI	CCCAGC	2 cut(s)	55, 2027
BsgI	GTGCAG	2 cut(s)	819, 998
Bsh1285I	CGRYCG	2 cut(s)	815, 2102
BshNI	GGYRCC	2 cut(s)	638, 2012
BshTI	ACCGGT	2 cut(s)	641, 2272
BshVI	ATCGAT	1 cut(s)	1446
BsiEI	CGRYCG	2 cut(s)	815, 2102
BsiHKAI	GWGCWC	2 cut(s)	589, 1276
BsiSI	CCGG	7 cut(s)	591, 642, 815, 1374, 1703, 2273, 2351
BslFI	GGGAC	5 cut(s)	526, 632, 1561, 1751, 2044
BslI	CCNNNNNNNGG	7 cut(s)	266, 995, 1304, 1336, 1374, 1661, 2082
BsmAI	GTCTC	1 cut(s)	1670
BsmFI	GGGAC	5 cut(s)	526, 632, 1561, 1751, 2044
BsmI	GAATGC	2 cut(s)	1215, 1690
Bsp1286I	GDGCHC	3 cut(s)	589, 636, 1276
Bsp1407I	TGTACA	1 cut(s)	1066
Bsp19I	CCATGG	1 cut(s)	889
BspACI	CCGC	4 cut(s)	1055, 1251, 1473, 1975
BspDI	ATCGAT	1 cut(s)	1446
BspLI	GGNNCC	5 cut(s)	640, 998, 1059, 2014, 2383
BspPI	GGATC	9 cut(s)	127, 154, 677, 871, 1052, 1065, 1442, 2150, 2474
BspT107I	GGYRCC	2 cut(s)	638, 2012
BspTI	CTTAAG	1 cut(s)	2053
BsrDI	GCAATG	3 cut(s)	709, 930, 1638
BsrFI	RCCGGY	3 cut(s)	641, 814, 2272
BsrGI	TGTACA	1 cut(s)	1066
BsrI	ACTGG	3 cut(s)	614, 778, 1985
BssAI	RCCGGY	3 cut(s)	641, 814, 2272
BssECI	CCNNGG	8 cut(s)	102, 650, 651, 889, 1202, 2096, 2305, 2511
BssNAI	GTATAC	1 cut(s)	783
BssNI	GRCGYC	1 cut(s)	2013
BssSI	CACGAG	2 cut(s)	47, 1290
BssT1I	CCWWGG	3 cut(s)	102, 889, 1202
Bst1107I	GTATAC	1 cut(s)	783
Bst2BI	CACGAG	2 cut(s)	47, 1290
Bst2UI	CCWGG	5 cut(s)	267, 417, 652, 769, 2307
Bst6I	CTCTTC	2 cut(s)	1305, 2136
BstACI	GRCGYC	1 cut(s)	2013
BstAFI	CTTAAG	1 cut(s)	2053
BstAPI	GCANNNNNTGC	1 cut(s)	1136
BstAUI	TGTACA	1 cut(s)	1066
BstC8I	GCNNGC	6 cut(s)	327, 331, 700, 1507, 1986, 2250
BstDSI	CCRYGG	2 cut(s)	889, 2511
BstF5I	GGATG	1 cut(s)	226
BstH2I	RGCGCY	2 cut(s)	2016, 2361
BstHHI	GCGC	4 cut(s)	1267, 1630, 2015, 2360
BstMAI	GTCTC	1 cut(s)	1670
BstMCI	CGRYCG	2 cut(s)	815, 2102
BstNI	CCWGG	5 cut(s)	267, 417, 652, 769, 2307
BstNSI	RCATGY	1 cut(s)	2301
BstSCI	CCNGG	8 cut(s)	265, 415, 589, 650, 767, 1373, 1701, 2305
BstSFI	CTRYAG	1 cut(s)	2454
BstSLI	GKGCMC	2 cut(s)	589, 1276
BstV1I	GCAGC	6 cut(s)	27, 30, 626, 1327, 1870, 1941
BstV2I	GAAGAC	2 cut(s)	291, 1787
BstX2I	RGATCY	6 cut(s)	132, 146, 187, 1009, 1057, 2155
BstXI	CCANNNNNNTGG	1 cut(s)	756
BstYI	RGATCY	6 cut(s)	132, 146, 187, 1009, 1057, 2155
BstZ17I	GTATAC	1 cut(s)	783
Bsu15I	ATCGAT	1 cut(s)	1446
Bsu36I	CCTNAGG	1 cut(s)	2254
BsuI	GTATCC	2 cut(s)	431, 1491
BsuTUI	ATCGAT	1 cut(s)	1446
BtgI	CCRYGG	2 cut(s)	889, 2511
BtsCI	GGATG	1 cut(s)	226
BtsIMutI	CAGTG	5 cut(s)	603, 1062, 1236, 1357, 1813
Cac8I	GCNNGC	6 cut(s)	327, 331, 700, 1507, 1986, 2250
CaiI	CAGNNNCTG	2 cut(s)	1864, 2000
CfoI	GCGC	4 cut(s)	1267, 1630, 2015, 2360
Cfr10I	RCCGGY	3 cut(s)	641, 814, 2272
Cfr13I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
ClaI	ATCGAT	1 cut(s)	1446
Csp6I	GTAC	6 cut(s)	409, 601, 886, 1067, 1327, 1426
CspAI	ACCGGT	2 cut(s)	641, 2272
CviQI	GTAC	6 cut(s)	409, 601, 886, 1067, 1327, 1426
DinI	GGCGCC	1 cut(s)	2014
EaeI	YGGCCR	4 cut(s)	182, 907, 1507, 1972
Eam1104I	CTCTTC	2 cut(s)	1305, 2136
EarI	CTCTTC	2 cut(s)	1305, 2136
Eco130I	CCWWGG	3 cut(s)	102, 889, 1202
Eco147I	AGGCCT	2 cut(s)	333, 2281
Eco24I	GRGCYC	1 cut(s)	636
Eco47I	GGWCC	6 cut(s)	356, 443, 527, 563, 923, 1664
Eco57I	CTGAAG	1 cut(s)	425
Eco81I	CCTNAGG	1 cut(s)	2254
EcoO109I	RGGNCCY	1 cut(s)	923
EcoRI	GAATTC	1 cut(s)	1568
EcoRII	CCWGG	5 cut(s)	265, 415, 650, 767, 2305
EcoT14I	CCWWGG	3 cut(s)	102, 889, 1202
EcoT22I	ATGCAT	1 cut(s)	1188
EcoT38I	GRGCYC	1 cut(s)	636
EgeI	GGCGCC	1 cut(s)	2014
EheI	GGCGCC	1 cut(s)	2014
ErhI	CCWWGG	3 cut(s)	102, 889, 1202
FaqI	GGGAC	5 cut(s)	526, 632, 1561, 1751, 2044
FauNDI	CATATG	3 cut(s)	94, 1843, 2198
FblI	GTMKAC	1 cut(s)	782
Fnu4HI	GCNGC	8 cut(s)	41, 44, 615, 1341, 1859, 1930, 1975, 2527
FokI	GGATG	1 cut(s)	233
FriOI	GRGCYC	1 cut(s)	636
Fsp4HI	GCNGC	8 cut(s)	41, 44, 615, 1341, 1859, 1930, 1975, 2527
FspBI	CTAG	6 cut(s)	939, 1046, 1401, 1565, 1919, 2337
FspI	TGCGCA	1 cut(s)	1266
GlaI	GCGC	4 cut(s)	1266, 1629, 2014, 2359
GluI	GCNGC	8 cut(s)	41, 44, 615, 1341, 1859, 1930, 1975, 2527
GsaI	CCCAGC	2 cut(s)	59, 2031
GsuI	CTGGAG	2 cut(s)	939, 1137
HaeII	RGCGCY	2 cut(s)	2016, 2361
HapII	CCGG	7 cut(s)	591, 642, 815, 1374, 1703, 2273, 2351
HhaI	GCGC	4 cut(s)	1267, 1630, 2015, 2360
Hin1I	GRCGYC	1 cut(s)	2013
Hin6I	GCGC	4 cut(s)	1265, 1628, 2013, 2358
HinP1I	GCGC	4 cut(s)	1265, 1628, 2013, 2358
HincII	GTYRAC	1 cut(s)	1538
HindII	GTYRAC	1 cut(s)	1538
HindIII	AAGCTT	1 cut(s)	2368
HinfI	GANTC	9 cut(s)	98, 226, 454, 468, 517, 1311, 1406, 1792, 1831
HpaII	CCGG	7 cut(s)	591, 642, 815, 1374, 1703, 2273, 2351
HphI	GGTGA	6 cut(s)	255, 667, 1483, 1899, 2188, 2275
HpyAV	CCTTC	7 cut(s)	203, 270, 850, 1725, 1894, 2175, 2348
Hsp92I	GRCGYC	1 cut(s)	2013
HspAI	GCGC	4 cut(s)	1265, 1628, 2013, 2358
KasI	GGCGCC	1 cut(s)	2012
LmnI	GCTCC	5 cut(s)	682, 961, 1118, 1384, 2381
Lsp1109I	GCAGC	6 cut(s)	27, 30, 626, 1327, 1870, 1941
LweI	GCATC	5 cut(s)	664, 943, 1275, 2006, 2398
MaeI	CTAG	6 cut(s)	939, 1046, 1401, 1565, 1919, 2337
MaeIII	GTNAC	6 cut(s)	716, 1352, 1846, 2018, 2057, 2449
MboII	GAAGA	8 cut(s)	296, 1211, 1292, 1294, 1787, 2153, 2278, 2512
MflI	RGATCY	6 cut(s)	132, 146, 187, 1009, 1057, 2155
MhlI	GDGCHC	3 cut(s)	589, 636, 1276
MlsI	TGGCCA	2 cut(s)	184, 1509
MluNI	TGGCCA	2 cut(s)	184, 1509
Mly113I	GGCGCC	1 cut(s)	2013
MlyI	GAGTC	2 cut(s)	511, 1801
MmeI	TCCRAC	1 cut(s)	1362
Mox20I	TGGCCA	2 cut(s)	184, 1509
Mph1103I	ATGCAT	1 cut(s)	1188
MscI	TGGCCA	2 cut(s)	184, 1509
MseI	TTAA	7 cut(s)	117, 693, 873, 1440, 1712, 1776, 2054
Msp20I	TGGCCA	2 cut(s)	184, 1509
MspA1I	CMGCKG	2 cut(s)	1505, 1898
MspCI	CTTAAG	1 cut(s)	2053
MspI	CCGG	7 cut(s)	591, 642, 815, 1374, 1703, 2273, 2351
MspR9I	CCNGG	8 cut(s)	267, 417, 591, 652, 769, 1375, 1703, 2307
Mva1269I	GAATGC	2 cut(s)	1215, 1690
MvaI	CCWGG	5 cut(s)	267, 417, 652, 769, 2307
NarI	GGCGCC	1 cut(s)	2013
NciI	CCSGG	3 cut(s)	591, 1375, 1703
NcoI	CCATGG	1 cut(s)	889
NdeI	CATATG	3 cut(s)	94, 1843, 2198
NlaIV	GGNNCC	5 cut(s)	640, 998, 1059, 2014, 2383
NmeAIII	GCCGAG	1 cut(s)	615
NmuCI	GTSAC	3 cut(s)	716, 1352, 1846
NsbI	TGCGCA	1 cut(s)	1266
NsiI	ATGCAT	1 cut(s)	1188
NspI	RCATGY	1 cut(s)	2301
PasI	CCCWGGG	1 cut(s)	651
PceI	AGGCCT	2 cut(s)	333, 2281
PciI	ACATGT	1 cut(s)	2297
PctI	GAATGC	2 cut(s)	1215, 1690
PfeI	GAWTC	7 cut(s)	98, 226, 454, 468, 1311, 1406, 1831
PinAI	ACCGGT	2 cut(s)	641, 2272
PkrI	GCNGC	8 cut(s)	42, 45, 616, 1342, 1860, 1931, 1976, 2528
PleI	GAGTC	2 cut(s)	511, 1800
PluTI	GGCGCC	1 cut(s)	2016
PpsI	GAGTC	2 cut(s)	511, 1800
PpuMI	RGGWCCY	1 cut(s)	923
PscI	ACATGT	1 cut(s)	2297
PshBI	ATTAAT	3 cut(s)	117, 693, 873
PsiI	TTATAA	1 cut(s)	1245
Psp5II	RGGWCCY	1 cut(s)	923
Psp6I	CCWGG	5 cut(s)	265, 415, 650, 767, 2305
PspFI	CCCAGC	2 cut(s)	55, 2027
PspGI	CCWGG	5 cut(s)	265, 415, 650, 767, 2305
PspN4I	GGNNCC	5 cut(s)	640, 998, 1059, 2014, 2383
PspPI	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
PspPPI	RGGWCCY	1 cut(s)	923
PstNI	CAGNNNCTG	2 cut(s)	1864, 2000
PsuI	RGATCY	6 cut(s)	132, 146, 187, 1009, 1057, 2155
PvuII	CAGCTG	2 cut(s)	1505, 1898
RsaI	GTAC	6 cut(s)	410, 602, 887, 1068, 1328, 1427
RsaNI	GTAC	6 cut(s)	409, 601, 886, 1067, 1327, 1426
SaqAI	TTAA	7 cut(s)	117, 693, 873, 1440, 1712, 1776, 2054
SatI	GCNGC	8 cut(s)	41, 44, 615, 1341, 1859, 1930, 1975, 2527
Sau96I	GGNCC	9 cut(s)	356, 443, 527, 563, 923, 997, 1664, 1700, 2303
SchI	GAGTC	2 cut(s)	511, 1801
ScrFI	CCNGG	8 cut(s)	267, 417, 591, 652, 769, 1375, 1703, 2307
SduI	GDGCHC	3 cut(s)	589, 636, 1276
SfaNI	GCATC	5 cut(s)	664, 943, 1275, 2006, 2398
SfcI	CTRYAG	1 cut(s)	2454
SfoI	GGCGCC	1 cut(s)	2014
SgrAI	CRCCGGYG	1 cut(s)	641
SinI	GGWCC	6 cut(s)	356, 443, 527, 563, 923, 1664
SmlI	CTYRAG	1 cut(s)	2053
SmoI	CTYRAG	1 cut(s)	2053
SpeI	ACTAGT	1 cut(s)	938
SseBI	AGGCCT	2 cut(s)	333, 2281
SsiI	CCGC	4 cut(s)	1055, 1251, 1473, 1975
SspDI	GGCGCC	1 cut(s)	2012
SspI	AATATT	1 cut(s)	275
SspMI	CTAG	6 cut(s)	939, 1046, 1401, 1565, 1919, 2337
StuI	AGGCCT	2 cut(s)	333, 2281
StyD4I	CCNGG	8 cut(s)	265, 415, 589, 650, 767, 1373, 1701, 2305
StyI	CCWWGG	3 cut(s)	102, 889, 1202
TaqI	TCGA	2 cut(s)	1446, 1824
TaqII	GACCGA	2 cut(s)	580, 2088
TatI	WGTACW	3 cut(s)	408, 600, 1066
TauI	GCSGC	1 cut(s)	1977
TfiI	GAWTC	7 cut(s)	98, 226, 454, 468, 1311, 1406, 1831
Tru1I	TTAA	7 cut(s)	117, 693, 873, 1440, 1712, 1776, 2054
Tru9I	TTAA	7 cut(s)	117, 693, 873, 1440, 1712, 1776, 2054
TscAI	CASTG	5 cut(s)	610, 1069, 1243, 1357, 1813
TseFI	GTSAC	3 cut(s)	716, 1352, 1846
TseI	GCWGC	7 cut(s)	40, 43, 614, 1340, 1858, 1929, 2526
Tsp45I	GTSAC	3 cut(s)	716, 1352, 1846
TspGWI	ACGGA	4 cut(s)	1320, 1572, 2363, 2500
TspRI	CASTG	5 cut(s)	610, 1069, 1243, 1357, 1813
Vha464I	CTTAAG	1 cut(s)	2053
VneI	GTGCAC	2 cut(s)	585, 1272
VpaK11BI	GGWCC	6 cut(s)	356, 443, 527, 563, 923, 1664
VspI	ATTAAT	3 cut(s)	117, 693, 873
XapI	RAATTY	7 cut(s)	901, 1111, 1460, 1568, 1600, 1903, 2467
XbaI	TCTAGA	2 cut(s)	1564, 1918
XceI	RCATGY	1 cut(s)	2301
XcmI	CCANNNNNNNNNTGG	2 cut(s)	521, 2297
XmiI	GTMKAC	1 cut(s)	782
XspI	CTAG	6 cut(s)	939, 1046, 1401, 1565, 1919, 2337
Zsp2I	ATGCAT	1 cut(s)	1188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.