AT3G22740

Homocysteine S-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002729
Physical Location & Seq
Reverse (-)
8032917 .. 8036005
3089 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22740.1

Sequence Viewer

Length: 1044 bp
ATGGGATCTTTCGTGAAAGAAGAAACGTCGTCGTTGATGACAGATTTTTTGGAGAAGTGCGGTGGTTATGCGGTGGTGGATGGAGGGTTTGCGACGGAGCTACAGCGCCACGGAGCTGATATCAATGATCCACTTTGGAGTGCTAAGTGCCTTATTACTTCTCCTCACCTTGTTACAAAGGTGCACTTGGATTACCTTGAATCTGGAGCAAATATCATCATAACAGCATCTTACCAGGCGACGATACAAGGTTTTGTGGCAAAAGGTCTATCGGTGGGAGAAGCAGAAAATTTACTTAGAAGGAGCGTTGAAATCACGTACGAGGCACGTGAGATTTTCTACAACAGATGCACCAAGGGTTCATGGGATTTCGCCTACGCTGGAAAAGCTTCCCGGAGACCAATTCTTGTCGCAGCTTCCGTTGGTAGCTATGGAGCTTATCTCGCTGACGGCTCCGAATACAGTGGGATATATGGAGATTCTGTGAGTAAGGAGACATTAAAGGATTTCCATAGAAGAAGAGTTCAAATCTTAGCAAAATCTGGAGCCGACCTAATAGCATTCGAGACTATTCCAAATAAGCTCGAAGCAGAGGCTTATGCGGATCTTCTTGAGGAGGAAGATATCGATATTCCGGCATGGTTCTCGTTCACTTCCAAGGACGGAGTCAGTGTGCCACGAGGAGATTCAGTTGTAGAATGTGCGAAAGTGGCAGATTCATGCAAGAACGTGGTGGCCATTGGAATCAATTGTACTGCACCAAGATACATTCATGCTTTAATTATCTCCTTGCGCCAGATGACACGCAAACCAATCGTTGTATACCCAAACAGCGGGGAAGTCTACGACGGTCTGAACAAGAAATGGATTAAGTCAGAGGGAGAGTCAGAGGAGGATTTTGTGTCGTACGTGAGTAAATGGAGAGATGCAGGAGCTTCTCTGTTTGGAGGATGTTGTCGGACTACTCCAAACACCATCAGAGCCATTGCTAAAGTTCTCTCCGACGAACCTTCTGCAGCATCAAAACCCAAATTCGGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

347

Amino Acids

37.88

Weight (kDa)

5.56

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 332 3.5e-80 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 822, 843
AciI CCGC 4 cut(s) 60, 71, 602, 834
AclWI GGATC 3 cut(s) 13, 122, 612
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 2 cut(s) 289, 1031
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 3 cut(s) 320, 754, 908
AfiI CCNNNNNNNGG 3 cut(s) 833, 1034, 1035
AgsI TTSAA 3 cut(s) 200, 311, 527
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 8 cut(s) 100, 116, 389, 416, 429, 437, 583, 935
AluI AGCT 8 cut(s) 100, 116, 389, 416, 429, 437, 583, 935
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 3 cut(s) 391, 488, 560
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 3 cut(s) 13, 122, 612
AoxI GGCC 1 cut(s) 735
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 413, 1016
ApoI RAATTY 2 cut(s) 289, 1031
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
Asp700I GAANNNNTTC 1 cut(s) 388
AspLEI GCGC 2 cut(s) 108, 795
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 186
BalI TGGCCA 1 cut(s) 737
BauI CACGAG 1 cut(s) 678
BbrPI CACGTG 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 425, 1028
BccI CCATC 2 cut(s) 74, 983
BceAI ACGGC 1 cut(s) 466
BcgI CGANNNNNNTGC 6 cut(s) 627, 661, 796, 830, 995, 1029
BciT130I CCWGG 1 cut(s) 236
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 3 cut(s) 391, 488, 560
BfmI CTRYAG 2 cut(s) 101, 1014
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 2 cut(s) 414, 1017
BlsI GCNGC 2 cut(s) 415, 1018
Bme1390I CCNGG 2 cut(s) 236, 394
BmiI GGNNCC 2 cut(s) 454, 547
BmrFI CCNGG 2 cut(s) 236, 394
BmsI GCATC 4 cut(s) 236, 338, 916, 1028
BplI GAGNNNNNCTC 2 cut(s) 426, 458
BpmI CTGGAG 2 cut(s) 225, 564
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 394
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 3 cut(s) 318, 329, 910
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 109, 354, 657
BsaXI ACNNNNNCTCC 6 cut(s) 468, 498, 657, 675, 687, 705
Bsc4I CCNNNNNNNGG 3 cut(s) 833, 1034, 1035
Bse3DI GCAATG 1 cut(s) 984
BseBI CCWGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 3 cut(s) 109, 354, 657
BseGI GGATG 2 cut(s) 85, 956
BseLI CCNNNNNNNGG 3 cut(s) 833, 1034, 1035
BseMI GCAATG 1 cut(s) 984
BseRI GAGGAG 4 cut(s) 153, 629, 696, 905
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 2 cut(s) 425, 1028
BsgI GTGCAG 1 cut(s) 741
BshFI GGCC 1 cut(s) 737
BshVI ATCGAT 1 cut(s) 627
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 2 cut(s) 394, 635
BsiWI CGTACG 2 cut(s) 318, 906
BslI CCNNNNNNNGG 3 cut(s) 833, 1034, 1035
BsmAI GTCTC 3 cut(s) 391, 488, 560
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 1 cut(s) 737
Bso31I GGTCTC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 5, 127, 604
BspACI CCGC 4 cut(s) 60, 71, 602, 834
BspANI GGCC 1 cut(s) 737
BspDI ATCGAT 1 cut(s) 627
BspLI GGNNCC 2 cut(s) 454, 547
BspMAI CTGCAG 1 cut(s) 1018
BspPI GGATC 3 cut(s) 13, 122, 612
BspTNI GGTCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 984
BssECI CCNNGG 3 cut(s) 109, 354, 657
BssMI GATC 3 cut(s) 5, 127, 604
BssNAI GTATAC 1 cut(s) 823
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 2 cut(s) 354, 657
Bst1107I GTATAC 1 cut(s) 823
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 2 cut(s) 464, 851
Bst6I CTCTTC 1 cut(s) 514
BstBAI YACGTR 3 cut(s) 318, 329, 910
BstDEI CTNAG 3 cut(s) 144, 296, 532
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 85, 956
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 795
BstKTI GATC 3 cut(s) 8, 130, 607
BstMAI GTCTC 3 cut(s) 391, 488, 560
BstMBI GATC 3 cut(s) 5, 127, 604
BstMWI GCNNNNNNNGC 3 cut(s) 386, 443, 710
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 234, 392
BstSFI CTRYAG 2 cut(s) 101, 1014
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 425, 1028
BstX2I RGATCY 2 cut(s) 5, 604
BstYI RGATCY 2 cut(s) 5, 604
BstZ17I GTATAC 1 cut(s) 823
Bsu15I ATCGAT 1 cut(s) 627
BsuRI GGCC 1 cut(s) 737
BsuTUI ATCGAT 1 cut(s) 627
BtgI CCRYGG 1 cut(s) 109
BtsCI GGATG 2 cut(s) 85, 956
BtsIMutI CAGTG 2 cut(s) 469, 676
CfoI GCGC 2 cut(s) 108, 795
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 3 cut(s) 319, 753, 907
CviAII CATG 4 cut(s) 363, 639, 720, 773
CviQI GTAC 3 cut(s) 319, 753, 907
DdeI CTNAG 3 cut(s) 144, 296, 532
DpnI GATC 3 cut(s) 7, 129, 606
DpnII GATC 3 cut(s) 5, 127, 604
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Eco130I CCWWGG 2 cut(s) 354, 657
Eco31I GGTCTC 1 cut(s) 391
Eco32I GATATC 2 cut(s) 121, 625
Eco72I CACGTG 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 234
EcoRV GATATC 2 cut(s) 121, 625
EcoT14I CCWWGG 2 cut(s) 354, 657
ErhI CCWWGG 2 cut(s) 354, 657
FaeI CATG 4 cut(s) 366, 642, 723, 776
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 4 cut(s) 362, 638, 719, 772
FauI CCCGC 1 cut(s) 827
FblI GTMKAC 2 cut(s) 822, 843
Fnu4HI GCNGC 2 cut(s) 414, 1017
FokI GGATG 2 cut(s) 92, 963
Fsp4HI GCNGC 2 cut(s) 414, 1017
GlaI GCGC 2 cut(s) 107, 794
GluI GCNGC 2 cut(s) 414, 1017
GsuI CTGGAG 2 cut(s) 225, 564
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 737
HapII CCGG 2 cut(s) 394, 635
HhaI GCGC 2 cut(s) 108, 795
Hin1II CATG 4 cut(s) 366, 642, 723, 776
Hin6I GCGC 2 cut(s) 106, 793
HinP1I GCGC 2 cut(s) 106, 793
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 7 cut(s) 200, 479, 666, 686, 716, 744, 884
HpaII CCGG 2 cut(s) 394, 635
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 4 cut(s) 184, 651, 823, 844
Hpy188I TCNGA 7 cut(s) 457, 855, 877, 889, 960, 980, 1003
Hpy188III TCNNGA 5 cut(s) 13, 204, 543, 565, 611
Hpy8I GTNNAC 4 cut(s) 184, 651, 823, 844
Hpy99I CGWCG 6 cut(s) 31, 34, 97, 244, 851, 1007
HpyAV CCTTC 2 cut(s) 294, 1020
HpyCH4III ACNGT 2 cut(s) 464, 851
HpyCH4IV ACGT 5 cut(s) 26, 317, 328, 729, 909
HpyCH4V TGCA 6 cut(s) 184, 351, 723, 758, 929, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 443, 710
HpyF3I CTNAG 3 cut(s) 144, 296, 532
HpySE526I ACGT 5 cut(s) 26, 317, 328, 729, 909
Hsp92II CATG 4 cut(s) 366, 642, 723, 776
HspAI GCGC 2 cut(s) 106, 793
Kzo9I GATC 3 cut(s) 5, 127, 604
LmnI GCTCC 8 cut(s) 97, 113, 206, 303, 434, 458, 545, 932
LpnPI CCDG 9 cut(s) 189, 221, 248, 366, 407, 528, 648, 809, 915
Lsp1109I GCAGC 2 cut(s) 425, 1028
LweI GCATC 4 cut(s) 236, 338, 916, 1028
MaeII ACGT 5 cut(s) 26, 317, 328, 729, 909
MaeIII GTNAC 1 cut(s) 172
MalI GATC 3 cut(s) 7, 129, 606
MboI GATC 3 cut(s) 5, 127, 604
MboII GAAGA 5 cut(s) 32, 528, 531, 599, 632
MfeI CAATTG 1 cut(s) 748
MflI RGATCY 2 cut(s) 5, 604
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 5 cut(s) 289, 402, 748, 780, 1031
MluNI TGGCCA 1 cut(s) 737
MlyI GAGTC 2 cut(s) 675, 893
MmeI TCCRAC 2 cut(s) 938, 1026
Mox20I TGGCCA 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 388
MscI TGGCCA 1 cut(s) 737
MseI TTAA 3 cut(s) 500, 779, 870
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 834
MspI CCGG 2 cut(s) 394, 635
MspR9I CCNGG 2 cut(s) 236, 394
MunI CAATTG 1 cut(s) 748
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 386, 443, 710
NciI CCSGG 1 cut(s) 394
NdeII GATC 3 cut(s) 5, 127, 604
NlaIII CATG 4 cut(s) 366, 642, 723, 776
NlaIV GGNNCC 2 cut(s) 454, 547
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 5 cut(s) 200, 479, 686, 716, 744
Pfl23II CGTACG 2 cut(s) 318, 906
PflFI GACNNNGTC 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 392
PkrI GCNGC 2 cut(s) 415, 1018
PleI GAGTC 2 cut(s) 674, 892
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 2 cut(s) 674, 892
Ppu21I YACGTR 3 cut(s) 318, 329, 910
Psp6I CCWGG 1 cut(s) 234
PspCI CACGTG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 234
PspLI CGTACG 2 cut(s) 318, 906
PspN4I GGNNCC 2 cut(s) 454, 547
PstI CTGCAG 1 cut(s) 1018
PsuI RGATCY 2 cut(s) 5, 604
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 3 cut(s) 320, 754, 908
RsaNI GTAC 3 cut(s) 319, 753, 907
SaqAI TTAA 3 cut(s) 500, 779, 870
SatI GCNGC 2 cut(s) 414, 1017
Sau3AI GATC 3 cut(s) 5, 127, 604
SchI GAGTC 2 cut(s) 675, 893
ScrFI CCNGG 2 cut(s) 236, 394
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 4 cut(s) 236, 338, 916, 1028
SfcI CTRYAG 2 cut(s) 101, 1014
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 5 cut(s) 289, 402, 748, 780, 1031
SsiI CCGC 4 cut(s) 60, 71, 602, 834
StyD4I CCNGG 2 cut(s) 234, 392
StyI CCWWGG 2 cut(s) 354, 657
TaaI ACNGT 2 cut(s) 464, 851
TaiI ACGT 5 cut(s) 29, 320, 331, 732, 912
TaqI TCGA 3 cut(s) 564, 585, 627
TasI AATT 5 cut(s) 289, 402, 748, 780, 1031
TatI WGTACW 1 cut(s) 752
TfiI GAWTC 5 cut(s) 200, 479, 686, 716, 744
Tru1I TTAA 3 cut(s) 500, 779, 870
Tru9I TTAA 3 cut(s) 500, 779, 870
TscAI CASTG 2 cut(s) 469, 676
TseI GCWGC 2 cut(s) 413, 1016
TspDTI ATGAA 3 cut(s) 351, 708, 761
TspGWI ACGGA 4 cut(s) 110, 126, 409, 678
TspRI CASTG 2 cut(s) 469, 676
Tth111I GACNNNGTC 1 cut(s) 665
VneI GTGCAC 1 cut(s) 182
XapI RAATTY 2 cut(s) 289, 1031
XmiI GTMKAC 2 cut(s) 822, 843
XmnI GAANNNNTTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.