MD00G1181400.v1.1

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002729
Physical Location & Seq
Forward (+)
42684814 .. 42689305
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1181400.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGCTTCAACTTCAGGAAGGTGGCCACATCCATTATTCAAGAAGCCGCTTCGGTTACAAAAACAGAAAATACGCGGAGACCTTCTGCTACCCCATACAACTACAGCACACCTATAGCACTATGGGTTTAGGAAGCAAGGAAACGTCGTCGTTCCTGTCTGATTTCCTAGAAAAATGTGGTGGCTATGCTGTCCTCGACGGCGGTTTTGCTACGGAGCTTGAGCGGCATGGAGCTGACCTCAACGACCCTCTTTGGAGCGCCAAATGCCTTATCACTTCCCCTCACCTTGTTAGACGGGTACACTTGGACTACCTTGATGCCGGTGCAAACATCATACTAACCGCATCTTATCAGGCCACCATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAGGAAGAAGCCAAAGATTTGCTTAGGAAAAGTGTTGAAATTGCAATTGAGGCGCGAGAAATTTACTATGATAAGTTTCAATCAAGACGTCCAGTTCTAGTTGCAGCATCTCTCGGCAGCTATGGCGCTTACTTGGCTGATGGTTCCGAATATAGTGGGAACTATGGAGATGCGATAACTGTAGAAACATTGAAGGATTTCCACAGGGAAAGGGTACAGATTCTGGCCAATTCTGGTGCTGATCTGATCGCATTTGAGACGATTCCAAATAAGATAGAAGCAAAGGCATATGCTGAGCTTCTTGATGAAGAAGGAATAGAAATTCCAGCCTGGTTTTCCTTCTCTTCGAAGGATGGAATCAATGTGGTCAGCGGGGATTCCATGTTAGAATGTGCCTCCATTGCAGATTCATGCAAGAACGTTGTTGCTGTTGGAATCAACTGCACACCTCCTAGATTTATCTACGGACTGGTTTCATCTATTCGGAATGTTACAAACAAACCAATAGTGATATACCCTAACAGTGGTGAGACTTATGATGGTCAGACCAAGCAGTGGGTGAAATCGACTGGTGTGGTTAATGAAGAGTTTGCAGACATAGCAATAGACAAGTGGTGTGAGGCTGGGGCTTCTCTATTTGGAGGCTGCTGCAGGACTACTCCAAATACCATCAGAGCCATATCTAAAACTCTCTCCAACAAGTCTTCTTCTACCGTTAACGATGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

374

Amino Acids

40.95

Weight (kDa)

5.31

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 64 - 360 3.1e-83 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 481
AccB7I CCANNNNNTGG 1 cut(s) 945
AccBSI CCGCTC 1 cut(s) 223
AccII CGCG 2 cut(s) 74, 445
AciI CCGC 6 cut(s) 46, 74, 201, 223, 342, 762
AclI AACGTT 1 cut(s) 810
AcoI YGGCCR 2 cut(s) 22, 615
AcsI RAATTY 2 cut(s) 450, 711
AcyI GRCGYC 1 cut(s) 478
AfaI GTAC 2 cut(s) 300, 606
AfiI CCNNNNNNNGG 2 cut(s) 914, 945
AgsI TTSAA 5 cut(s) 8, 39, 428, 470, 583
AjnI CCWGG 1 cut(s) 719
AluBI AGCT 4 cut(s) 217, 233, 510, 688
AluI AGCT 4 cut(s) 217, 233, 510, 688
Alw26I GTCTC 3 cut(s) 71, 641, 914
AlwNI CAGNNNCTG 1 cut(s) 613
AoxI GGCC 4 cut(s) 22, 354, 375, 615
ApeKI GCWGC 4 cut(s) 494, 507, 1035, 1038
ApoI RAATTY 2 cut(s) 450, 711
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 587
AspLEI GCGC 3 cut(s) 260, 445, 518
AsuHPI GGTGA 3 cut(s) 275, 929, 961
AsuII TTCGAA 1 cut(s) 737
BalI TGGCCA 2 cut(s) 24, 617
BbsI GAAGAC 1 cut(s) 1086
BbvI GCAGC 4 cut(s) 506, 519, 1022, 1025
BccI CCATC 4 cut(s) 524, 737, 923, 1067
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 71, 641, 914
BfaI CTAG 3 cut(s) 167, 488, 843
BfmI CTRYAG 4 cut(s) 101, 112, 570, 1039
BfoI RGCGCY 2 cut(s) 261, 519
BisI GCNGC 6 cut(s) 46, 224, 495, 508, 1036, 1039
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 6 cut(s) 47, 225, 496, 509, 1037, 1040
Bme1390I CCNGG 1 cut(s) 721
BmiI GGNNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 721
BmsI GCATC 5 cut(s) 307, 353, 506, 550, 1105
BpiI GAAGAC 1 cut(s) 1086
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu10I CCTNAGC 1 cut(s) 413
Bpu1102I GCTNAGC 1 cut(s) 684
Bpu14I TTCGAA 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 239
BsaHI GRCGYC 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 71
Bsc4I CCNNNNNNNGG 2 cut(s) 914, 945
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 3 cut(s) 482, 864, 964
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 721
BseGI GGATG 2 cut(s) 27, 748
BseLI CCNNNNNNNGG 2 cut(s) 914, 945
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 3 cut(s) 482, 864, 964
BseXI GCAGC 4 cut(s) 506, 519, 1022, 1025
BseYI CCCAGC 1 cut(s) 1013
BsgI GTGCAG 1 cut(s) 817
Bsh1236I CGCG 2 cut(s) 74, 445
BshFI GGCC 4 cut(s) 24, 356, 377, 617
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 914, 945
BsmAI GTCTC 3 cut(s) 71, 641, 914
BsmBI CGTCTC 1 cut(s) 641
BsnI GGCC 4 cut(s) 24, 356, 377, 617
Bso31I GGTCTC 1 cut(s) 71
Bsp119I TTCGAA 1 cut(s) 737
Bsp143I GATC 2 cut(s) 631, 636
Bsp1720I GCTNAGC 1 cut(s) 684
BspACI CCGC 6 cut(s) 46, 74, 201, 223, 342, 762
BspANI GGCC 4 cut(s) 24, 356, 377, 617
BspCNI CTCAG 1 cut(s) 676
BspFNI CGCG 2 cut(s) 74, 445
BspLI GGNNCC 1 cut(s) 535
BspMAI CTGCAG 1 cut(s) 1043
BspT104I TTCGAA 1 cut(s) 737
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 3 cut(s) 482, 864, 964
BssAI RCCGGY 1 cut(s) 320
BssMI GATC 2 cut(s) 631, 636
BssNI GRCGYC 1 cut(s) 478
Bst2UI CCWGG 1 cut(s) 721
Bst4CI ACNGT 3 cut(s) 571, 914, 1105
Bst6I CTCTTC 2 cut(s) 739, 969
BstACI GRCGYC 1 cut(s) 478
BstBI TTCGAA 1 cut(s) 737
BstDEI CTNAG 3 cut(s) 390, 413, 684
BstF5I GGATG 2 cut(s) 27, 748
BstFNI CGCG 2 cut(s) 74, 445
BstH2I RGCGCY 2 cut(s) 261, 519
BstHHI GCGC 3 cut(s) 260, 445, 518
BstKTI GATC 2 cut(s) 634, 639
BstMAI GTCTC 3 cut(s) 71, 641, 914
BstMBI GATC 2 cut(s) 631, 636
BstMWI GCNNNNNNNGC 7 cut(s) 223, 264, 440, 513, 524, 791, 989
BstNI CCWGG 1 cut(s) 721
BstSCI CCNGG 1 cut(s) 719
BstSFI CTRYAG 4 cut(s) 101, 112, 570, 1039
BstUI CGCG 2 cut(s) 74, 445
BstV1I GCAGC 4 cut(s) 506, 519, 1022, 1025
BstV2I GAAGAC 1 cut(s) 1086
BsuRI GGCC 4 cut(s) 24, 356, 377, 617
BtsCI GGATG 2 cut(s) 27, 748
BtsI GCAGTG 1 cut(s) 950
BtsIMutI CAGTG 2 cut(s) 919, 950
CaiI CAGNNNCTG 1 cut(s) 613
CfoI GCGC 3 cut(s) 260, 445, 518
Cfr10I RCCGGY 1 cut(s) 320
Csp6I GTAC 2 cut(s) 299, 605
CviAII CATG 3 cut(s) 227, 772, 801
CviQI GTAC 2 cut(s) 299, 605
DdeI CTNAG 3 cut(s) 390, 413, 684
DpnI GATC 2 cut(s) 633, 638
DpnII GATC 2 cut(s) 631, 636
EaeI YGGCCR 2 cut(s) 22, 615
Eam1104I CTCTTC 2 cut(s) 739, 969
EarI CTCTTC 2 cut(s) 739, 969
Eco31I GGTCTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 719
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 3 cut(s) 230, 775, 804
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 3 cut(s) 226, 771, 800
FauI CCCGC 1 cut(s) 755
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 6 cut(s) 46, 224, 495, 508, 1036, 1039
FokI GGATG 2 cut(s) 14, 755
Fsp4HI GCNGC 6 cut(s) 46, 224, 495, 508, 1036, 1039
FspBI CTAG 3 cut(s) 167, 488, 843
GlaI GCGC 3 cut(s) 259, 444, 517
GluI GCNGC 6 cut(s) 46, 224, 495, 508, 1036, 1039
GsaI CCCAGC 1 cut(s) 1017
HaeII RGCGCY 2 cut(s) 261, 519
HaeIII GGCC 4 cut(s) 24, 356, 377, 617
HapII CCGG 1 cut(s) 321
HhaI GCGC 3 cut(s) 260, 445, 518
Hin1I GRCGYC 1 cut(s) 478
Hin1II CATG 3 cut(s) 230, 775, 804
Hin6I GCGC 3 cut(s) 258, 443, 516
HinP1I GCGC 3 cut(s) 258, 443, 516
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HinfI GANTC 6 cut(s) 610, 652, 747, 767, 797, 825
HpaI GTTAAC 1 cut(s) 1108
HpaII CCGG 1 cut(s) 321
HphI GGTGA 3 cut(s) 275, 929, 961
Hpy166II GTNNAC 2 cut(s) 301, 1108
Hpy188I TCNGA 6 cut(s) 160, 538, 636, 876, 936, 1064
Hpy188III TCNNGA 4 cut(s) 14, 39, 474, 692
Hpy8I GTNNAC 2 cut(s) 301, 1108
Hpy99I CGWCG 3 cut(s) 148, 151, 200
HpyAV CCTTC 6 cut(s) 11, 91, 577, 695, 733, 739
HpyCH4III ACNGT 3 cut(s) 571, 914, 1105
HpyCH4IV ACGT 3 cut(s) 143, 478, 810
HpyCH4V TGCA 8 cut(s) 326, 434, 494, 794, 804, 834, 983, 1041
HpyF10VI GCNNNNNNNGC 7 cut(s) 223, 264, 440, 513, 524, 791, 989
HpyF3I CTNAG 3 cut(s) 390, 413, 684
HpySE526I ACGT 3 cut(s) 143, 478, 810
Hsp92I GRCGYC 1 cut(s) 478
Hsp92II CATG 3 cut(s) 230, 775, 804
HspAI GCGC 3 cut(s) 258, 443, 516
KspAI GTTAAC 1 cut(s) 1108
Kzo9I GATC 2 cut(s) 631, 636
LmnI GCTCC 3 cut(s) 214, 230, 255
Lsp1109I GCAGC 4 cut(s) 506, 519, 1022, 1025
LweI GCATC 5 cut(s) 307, 353, 506, 550, 1105
MaeI CTAG 3 cut(s) 167, 488, 843
MaeII ACGT 3 cut(s) 143, 478, 810
MaeIII GTNAC 2 cut(s) 53, 880
MalI GATC 2 cut(s) 633, 638
MbiI CCGCTC 1 cut(s) 223
MboI GATC 2 cut(s) 631, 636
MboII GAAGA 6 cut(s) 407, 710, 726, 986, 1086, 1089
MfeI CAATTG 1 cut(s) 435
MlsI TGGCCA 2 cut(s) 24, 617
MluCI AATT 5 cut(s) 429, 435, 450, 619, 711
MluNI TGGCCA 2 cut(s) 24, 617
MmeI TCCRAC 2 cut(s) 802, 1110
Mox20I TGGCCA 2 cut(s) 24, 617
MroXI GAANNNNTTC 1 cut(s) 587
MscI TGGCCA 2 cut(s) 24, 617
MseI TTAA 3 cut(s) 969, 1107, 1120
Msp20I TGGCCA 2 cut(s) 24, 617
MspA1I CMGCKG 1 cut(s) 762
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 721
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 721
MvnI CGCG 2 cut(s) 74, 445
MwoI GCNNNNNNNGC 7 cut(s) 223, 264, 440, 513, 524, 791, 989
NdeI CATATG 1 cut(s) 679
NdeII GATC 2 cut(s) 631, 636
NlaIII CATG 3 cut(s) 230, 775, 804
NlaIV GGNNCC 1 cut(s) 535
NmeAIII GCCGAG 1 cut(s) 483
NspV TTCGAA 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 6 cut(s) 610, 652, 747, 767, 797, 825
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 6 cut(s) 47, 225, 496, 509, 1037, 1040
Psp1406I AACGTT 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 1013
PspGI CCWGG 1 cut(s) 719
PspN4I GGNNCC 1 cut(s) 535
PstI CTGCAG 1 cut(s) 1043
PstNI CAGNNNCTG 1 cut(s) 613
RsaI GTAC 2 cut(s) 300, 606
RsaNI GTAC 2 cut(s) 299, 605
SaqAI TTAA 3 cut(s) 969, 1107, 1120
SatI GCNGC 6 cut(s) 46, 224, 495, 508, 1036, 1039
Sau3AI GATC 2 cut(s) 631, 636
ScrFI CCNGG 1 cut(s) 721
SfaNI GCATC 5 cut(s) 307, 353, 506, 550, 1105
SfcI CTRYAG 4 cut(s) 101, 112, 570, 1039
SfuI TTCGAA 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 5 cut(s) 429, 435, 450, 619, 711
SsiI CCGC 6 cut(s) 46, 74, 201, 223, 342, 762
SspMI CTAG 3 cut(s) 167, 488, 843
StyD4I CCNGG 1 cut(s) 719
TaaI ACNGT 3 cut(s) 571, 914, 1105
TaiI ACGT 3 cut(s) 146, 481, 813
TaqI TCGA 3 cut(s) 195, 737, 956
TasI AATT 5 cut(s) 429, 435, 450, 619, 711
TauI GCSGC 2 cut(s) 48, 226
TfiI GAWTC 6 cut(s) 610, 652, 747, 767, 797, 825
Tru1I TTAA 3 cut(s) 969, 1107, 1120
Tru9I TTAA 3 cut(s) 969, 1107, 1120
TscAI CASTG 2 cut(s) 919, 950
TseI GCWGC 4 cut(s) 494, 507, 1035, 1038
TspDTI ATGAA 4 cut(s) 711, 789, 855, 987
TspGWI ACGGA 2 cut(s) 227, 870
TspRI CASTG 2 cut(s) 919, 950
Van91I CCANNNNNTGG 1 cut(s) 945
XapI RAATTY 2 cut(s) 450, 711
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 3 cut(s) 167, 488, 843
ZraI GACGTC 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.