RchiOBHm_Chr4g0387451

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Forward (+)
2649779 .. 2659146
9368 bp
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UTR
Exon/CDS
Intron
PRQ36077

Sequence Viewer

Length: 471 bp
ATGAAGGAAAATTGTAGAATTGCAATCGAGGCATGCGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGAAATTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCGGCATCTGTGGGAAGCTGTGGAGCCTATTTGGCTGATGGTTCCGAGTATAGTGGCAACTGTGGCGATGCAGTGACTCTAGAAGCATTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGTTGCTCATCTAATTGCATATGAGACGACACCCAATAAATTAGAGGCAAAGGCATATGCTGAACTTCTTGAGGAGGAAGCATTAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGTTGGAATCAGTGTGGTGAGTGGTGATTCCATCTTTGAGTGTGCCTCTATTGCTAATGTGTGGAAGCAAGTTGTTGTTGTTGGAATCTACTGTACACTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

156

Amino Acids

16.93

Weight (kDa)

4.83

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 4 - 156 4.8e-22 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 119
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 39
AfaI GTAC 1 cut(s) 460
AgsI TTSAA 2 cut(s) 99, 208
AluBI AGCT 1 cut(s) 135
AluI AGCT 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 238
AoxI GGCC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 3 cut(s) 94, 212, 228
AsuHPI GGTGA 2 cut(s) 394, 401
BalI TGGCCA 1 cut(s) 242
BccI CCATC 2 cut(s) 149, 404
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 2 cut(s) 113, 197
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 142, 160
BmsI GCATC 2 cut(s) 131, 175
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpuEI CTTGAG 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 1 cut(s) 335
BshFI GGCC 1 cut(s) 242
BsmAI GTCTC 1 cut(s) 266
BsmBI CGTCTC 1 cut(s) 266
BsnI GGCC 1 cut(s) 242
Bsp1407I TGTACA 1 cut(s) 458
BspACI CCGC 1 cut(s) 119
BspANI GGCC 1 cut(s) 242
BspLI GGNNCC 2 cut(s) 142, 160
BsrGI TGTACA 1 cut(s) 458
BsrI ACTGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 179, 458
BstAUI TGTACA 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 34
BstF5I GGATG 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 266
BstMWI GCNNNNNNNGC 4 cut(s) 29, 149, 180, 416
BstNSI RCATGY 1 cut(s) 36
BsuRI GGCC 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 1 cut(s) 103
BtsI GCAGTG 2 cut(s) 195, 461
BtsIMutI CAGTG 3 cut(s) 195, 382, 461
Cac8I GCNNGC 1 cut(s) 34
CaiI CAGNNNCTG 1 cut(s) 238
Csp6I GTAC 1 cut(s) 459
CviAII CATG 2 cut(s) 33, 345
CviJI RGCY 5 cut(s) 94, 135, 143, 152, 242
CviKI_1 RGCY 5 cut(s) 94, 135, 143, 152, 242
CviQI GTAC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 266
FaeI CATG 2 cut(s) 36, 348
FaiI YATR 8 cut(s) 34, 48, 168, 268, 270, 304, 306, 346
FatI CATG 2 cut(s) 32, 344
FauNDI CATATG 2 cut(s) 268, 304
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 2 cut(s) 113, 197
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 242
Hin1II CATG 2 cut(s) 36, 348
HinfI GANTC 5 cut(s) 193, 235, 372, 392, 450
HphI GGTGA 2 cut(s) 394, 401
Hpy166II GTNNAC 3 cut(s) 82, 351, 461
Hpy188I TCNGA 2 cut(s) 163, 234
Hpy188III TCNNGA 3 cut(s) 99, 197, 317
Hpy8I GTNNAC 3 cut(s) 82, 351, 461
HpyAV CCTTC 1 cut(s) 105
HpyCH4III ACNGT 2 cut(s) 179, 458
HpyCH4V TGCA 4 cut(s) 23, 57, 188, 266
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 149, 180, 416
Hsp92II CATG 2 cut(s) 36, 348
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 5 cut(s) 72, 120, 205, 224, 354
LweI GCATC 2 cut(s) 131, 175
MaeI CTAG 2 cut(s) 113, 197
MaeIII GTNAC 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 6 cut(s) 10, 18, 39, 75, 261, 287
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 2 cut(s) 349, 427
MnlI CCTC 5 cut(s) 22, 286, 313, 316, 421
Mox20I TGGCCA 1 cut(s) 242
MroXI GAANNNNTTC 3 cut(s) 94, 212, 228
MscI TGGCCA 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 242
MwoI GCNNNNNNNGC 4 cut(s) 29, 149, 180, 416
NdeI CATATG 2 cut(s) 268, 304
NlaIII CATG 2 cut(s) 36, 348
NlaIV GGNNCC 2 cut(s) 142, 160
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 36
PaeI GCATGC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 3 cut(s) 94, 212, 228
PfeI GAWTC 4 cut(s) 235, 372, 392, 450
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 142, 160
PstNI CAGNNNCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SatI GCNGC 1 cut(s) 120
SchI GAGTC 1 cut(s) 187
SetI ASST 2 cut(s) 67, 137
SfaNI GCATC 2 cut(s) 131, 175
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 1 cut(s) 36
Sse9I AATT 6 cut(s) 10, 18, 39, 75, 261, 287
SsiI CCGC 1 cut(s) 119
SspMI CTAG 2 cut(s) 113, 197
TaaI ACNGT 2 cut(s) 179, 458
TaqI TCGA 1 cut(s) 27
TasI AATT 6 cut(s) 10, 18, 39, 75, 261, 287
TatI WGTACW 1 cut(s) 458
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 4 cut(s) 235, 372, 392, 450
TscAI CASTG 3 cut(s) 195, 382, 468
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 195, 382, 468
XapI RAATTY 1 cut(s) 39
XbaI TCTAGA 1 cut(s) 196
XceI RCATGY 1 cut(s) 36
XmnI GAANNNNTTC 3 cut(s) 94, 212, 228
XspI CTAG 2 cut(s) 113, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.