Rorug03G0309400

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002729
Physical Location & Seq
Forward (+)
32970647 .. 32974437
3791 bp
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UTR
Exon/CDS
Intron
Rorug03G0309400.1

Sequence Viewer

Length: 450 bp
ATGGCCGATCAGCTCACCGACGACCAGATCTCTGAGTTCAAGGAGGCGTTCAGCCTCTTCGACAAGGACGGCGATGGCTGCATCACTACCAAGGAGCTTGGCACTGTCATGCGGTCACTTGGGCAGAACCCAACCGAAGCAGAGCTTCAGGATATGATTAATGAGGTTGATGCTGATGGGAATGGTACCATTGATTTCCCAGAGTTCCTTAACCTGATGGCTCGAAAGATGAAGGACACTGATTCTGAGGAGGAGCTCAAGGAAGCGTTCCGGGTGTTCGATAAGGACCAGAATGGCTTCATTTCTGCTGCTGAGCTTCGTCATGTTATGACAAATCTGGGTGAGAAGCTTACAGATGAGGAAGTTGATGAGATGATTCGCGAGGCTGATGTGGATGGTGATGGACAGATCAACTATGAGGAGTTCGTCAAAGTCATGATGGCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

149

Amino Acids

16.85

Weight (kDa)

4.11

Isoelectric Point (pI)

24.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 7 - 76 2.9e-08 Allograft inflammatory factor 1
EF-hand_1 PF00036 12 - 40 3.8e-09 EF hand domain
EF-hand_7 PF13499 12 - 73 1.8e-17 EF-hand domain pair
EF-hand_6 PF13405 12 - 41 2.7e-09 EF-hand domain
EF-hand_9 PF14658 15 - 75 5.5e-10 EF-hand domain
EF-hand_8 PF13833 25 - 75 7.3e-11 EF-hand domain pair
EF-hand_1 PF00036 48 - 75 3.2e-08 EF hand domain
EF-hand_7 PF13499 83 - 147 5.9e-22 EF-hand domain pair
AIF-1 PF21008 84 - 149 2.8e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 85 - 112 2.5e-09 EF hand domain
EF-hand_6 PF13405 85 - 114 2e-09 EF-hand domain
EF-hand_5 PF13202 86 - 110 1.3e-06 EF hand
EF-hand_9 PF14658 88 - 148 3.3e-07 EF-hand domain
EF-hand_8 PF13833 98 - 146 1.1e-12 EF-hand domain pair
EF-hand_1 PF00036 121 - 148 3.7e-10 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccII CGCG 1 cut(s) 381
AciI CCGC 1 cut(s) 112
AcoI YGGCCR 2 cut(s) 3, 441
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 1 cut(s) 187
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 6 cut(s) 13, 97, 145, 256, 316, 349
AluI AGCT 6 cut(s) 13, 97, 145, 256, 316, 349
Alw21I GWGCWC 1 cut(s) 258
AoxI GGCC 2 cut(s) 3, 441
ApeKI GCWGC 2 cut(s) 78, 308
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 296
Asp718I GGTACC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 7, 353, 410
AvaII GGWCC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 65, 295
BccI CCATC 6 cut(s) 68, 170, 211, 389, 395, 433
BceAI ACGGC 1 cut(s) 85
BcnI CCSGG 1 cut(s) 272
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 79, 309
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 2 cut(s) 80, 310
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 90, 160
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 400
BseMII CTCAG 3 cut(s) 24, 237, 303
BseRI GAGGAG 3 cut(s) 263, 266, 434
BseXI GCAGC 2 cut(s) 65, 295
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 2 cut(s) 5, 443
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 271
BsnI GGCC 2 cut(s) 5, 443
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 3 cut(s) 7, 27, 408
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp68I TCGCGA 1 cut(s) 381
BspACI CCGC 1 cut(s) 112
BspANI GGCC 2 cut(s) 5, 443
BspCNI CTCAG 3 cut(s) 25, 238, 304
BspFNI CGCG 1 cut(s) 381
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 3 cut(s) 7, 27, 408
BssT1I CCWWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 33, 246, 312
BstF5I GGATG 1 cut(s) 400
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 3 cut(s) 10, 30, 411
BstMBI GATC 3 cut(s) 7, 27, 408
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 65, 295
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BsuRI GGCC 2 cut(s) 5, 443
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 102, 237
BtuMI TCGCGA 1 cut(s) 381
CciI TCATGA 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 186
CviAII CATG 3 cut(s) 109, 323, 436
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 3 cut(s) 33, 246, 312
DpnI GATC 3 cut(s) 9, 29, 410
DpnII GATC 3 cut(s) 7, 27, 408
EaeI YGGCCR 2 cut(s) 3, 441
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 3 cut(s) 112, 326, 439
FaiI YATR 6 cut(s) 110, 155, 324, 329, 417, 437
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 3 cut(s) 108, 322, 435
Fnu4HI GCNGC 2 cut(s) 79, 309
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 79, 309
GluI GCNGC 2 cut(s) 79, 309
HaeIII GGCC 2 cut(s) 5, 443
HapII CCGG 1 cut(s) 271
Hin1II CATG 3 cut(s) 112, 326, 439
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 2 cut(s) 242, 376
HpaII CCGG 1 cut(s) 271
HphI GGTGA 3 cut(s) 7, 353, 410
Hpy188I TCNGA 2 cut(s) 34, 247
Hpy188III TCNNGA 3 cut(s) 149, 380, 436
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 3 cut(s) 33, 246, 312
Hsp92II CATG 3 cut(s) 112, 326, 439
KpnI GGTACC 1 cut(s) 189
Kzo9I GATC 3 cut(s) 7, 27, 408
LmnI GCTCC 2 cut(s) 94, 253
LpnPI CCDG 7 cut(s) 38, 134, 213, 227, 284, 302, 323
Lsp1109I GCAGC 2 cut(s) 65, 295
LweI GCATC 2 cut(s) 90, 160
MaeIII GTNAC 1 cut(s) 114
MalI GATC 3 cut(s) 9, 29, 410
MboI GATC 3 cut(s) 7, 27, 408
MboII GAAGA 1 cut(s) 49
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 443
MluNI TGGCCA 1 cut(s) 443
MnlI CCTC 8 cut(s) 37, 65, 157, 241, 244, 352, 376, 412
Mox20I TGGCCA 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 443
MseI TTAA 2 cut(s) 159, 210
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 443
MspI CCGG 1 cut(s) 271
MspR9I CCNGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 78
NciI CCSGG 1 cut(s) 272
NdeII GATC 3 cut(s) 7, 27, 408
NlaIII CATG 3 cut(s) 112, 326, 439
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 114
NruI TCGCGA 1 cut(s) 381
PagI TCATGA 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 242, 376
PkrI GCNGC 2 cut(s) 80, 310
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 1 cut(s) 286
PsuI RGATCY 1 cut(s) 27
RruI TCGCGA 1 cut(s) 381
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 159, 210
SatI GCNGC 2 cut(s) 79, 309
Sau3AI GATC 3 cut(s) 7, 27, 408
Sau96I GGNCC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 258
SetI ASST 8 cut(s) 15, 99, 147, 168, 216, 258, 318, 351
SfaNI GCATC 2 cut(s) 90, 160
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SsiI CCGC 1 cut(s) 112
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 3 cut(s) 60, 223, 279
TfiI GAWTC 2 cut(s) 242, 376
Tru1I TTAA 2 cut(s) 159, 210
Tru9I TTAA 2 cut(s) 159, 210
TscAI CASTG 2 cut(s) 109, 244
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 2 cut(s) 78, 308
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 2 cut(s) 245, 289
TspRI CASTG 2 cut(s) 109, 244
VpaK11BI GGWCC 1 cut(s) 286
VspI ATTAAT 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.