RchiOBHm_Chr4g0386841

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Reverse (-)
2185198 .. 2186895
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36019

Sequence Viewer

Length: 705 bp
ATGAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCTGAAGAAGAAGCCAAAGCCCTGCTCAGGAAAACCTTCAAGACATCCGGTTCTAATTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATTGTGGCAACTACGGCGATGCAGTGACTCTAGAAACATTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGGTGCTGATCTTATTGCATTTGAGACGACACCGAATAAGCTAGAGGCGAAGGCATATGCTGAACTTCTTGAGGAAGTGGCAATAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAATGTGGTGAGTGGTGATTCCATCTTTGAGTGTGCCTCTATTGCTAATGCATGCAAGCAAGTTGTTGCTGTTGGAATCAACTGCACACCACCTAGTTATATCCAGGGGTTCATTTCATCTATTCGGAAGGTAACAAGCAAACCAATAGTGATATACCCCAACAGGGGACGTGAGACTTATGATGGTCAGAGCAAGCAATGGATTTGGGCGGTTGATGAAGAGTTTTCAGAAATAGTCATAGAGAAGTGGCGTGCGGCTGGGGCGTCCCTGTTTGGAGGCTGCTGCGGAACAACTCCAAATACCATAAGGGCAATATCTAAGGCTCTTTCTAACAAGTCTTCTACAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

234

Amino Acids

25.46

Weight (kDa)

8.77

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 29 - 227 3.4e-50 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 563, 608, 639
AcoI YGGCCR 1 cut(s) 219
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 617
AfiI CCNNNNNNNGG 3 cut(s) 65, 517, 518
AgsI TTSAA 2 cut(s) 78, 187
AjiI CACGTC 1 cut(s) 524
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 2 cut(s) 114, 268
AluI AGCT 2 cut(s) 114, 268
Alw26I GTCTC 2 cut(s) 245, 521
AlwNI CAGNNNCTG 2 cut(s) 104, 217
AoxI GGCC 3 cut(s) 5, 26, 219
ApeKI GCWGC 3 cut(s) 98, 633, 636
Asp700I GAANNNNTTC 3 cut(s) 73, 191, 207
AsuHPI GGTGA 2 cut(s) 373, 380
BalI TGGCCA 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 110, 620, 623
BccI CCATC 4 cut(s) 128, 341, 383, 530
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 245, 521
BfaI CTAG 3 cut(s) 176, 269, 447
BglI GCCNNNNNGGC 1 cut(s) 128
BisI GCNGC 4 cut(s) 99, 609, 634, 637
BlsI GCNGC 4 cut(s) 100, 610, 635, 638
Bme1390I CCNGG 1 cut(s) 458
BmgBI CACGTC 1 cut(s) 524
BmiI GGNNCC 2 cut(s) 121, 139
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 110, 154
BpiI GAAGAC 1 cut(s) 684
BplI GAGNNNNNCTC 2 cut(s) 374, 406
Bpu10I CCTNAGC 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 617
BsaJI CCNNGG 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 517, 518
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 3 cut(s) 65, 517, 518
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 78
BseXI GCAGC 3 cut(s) 110, 620, 623
BseYI CCCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 421
BshFI GGCC 3 cut(s) 7, 28, 221
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 2 cut(s) 534, 604
BslI CCNNNNNNNGG 3 cut(s) 65, 517, 518
BsmAI GTCTC 2 cut(s) 245, 521
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 534, 604
BsnI GGCC 3 cut(s) 7, 28, 221
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 3 cut(s) 563, 608, 639
BspANI GGCC 3 cut(s) 7, 28, 221
BspCNI CTCAG 1 cut(s) 77
BspLI GGNNCC 2 cut(s) 121, 139
BsrDI GCAATG 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 1 cut(s) 235
BssNI GRCGYC 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 458
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 406, 410, 548, 606
BstDEI CTNAG 2 cut(s) 64, 672
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 2 cut(s) 245, 521
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 4 cut(s) 128, 159, 395, 614
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 110, 620, 623
BstV2I GAAGAC 1 cut(s) 684
BsuRI GGCC 3 cut(s) 7, 28, 221
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 524
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 4 cut(s) 406, 410, 548, 606
CaiI CAGNNNCTG 2 cut(s) 104, 217
CseI GACGC 1 cut(s) 606
CviAII CATG 2 cut(s) 324, 405
DdeI CTNAG 2 cut(s) 64, 672
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 406
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 2 cut(s) 327, 408
FaqI GGGAC 2 cut(s) 534, 604
FatI CATG 2 cut(s) 323, 404
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 99, 609, 634, 637
FokI GGATG 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 99, 609, 634, 637
FspBI CTAG 3 cut(s) 176, 269, 447
GluI GCNGC 4 cut(s) 99, 609, 634, 637
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 3 cut(s) 7, 28, 221
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 606
Hin1I GRCGYC 1 cut(s) 617
Hin1II CATG 2 cut(s) 327, 408
HinfI GANTC 5 cut(s) 172, 214, 351, 371, 429
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 373, 380
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 6 cut(s) 42, 142, 213, 480, 543, 583
Hpy188III TCNNGA 4 cut(s) 66, 78, 176, 296
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 3 cut(s) 84, 271, 475
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 6 cut(s) 98, 167, 245, 404, 408, 438
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 159, 395, 614
HpyF3I CTNAG 2 cut(s) 64, 672
HpySE526I ACGT 1 cut(s) 523
Hsp92I GRCGYC 1 cut(s) 617
Hsp92II CATG 2 cut(s) 327, 408
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 119
Lsp1109I GCAGC 3 cut(s) 110, 620, 623
LweI GCATC 2 cut(s) 110, 154
MaeI CTAG 3 cut(s) 176, 269, 447
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 2 cut(s) 169, 484
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 4 cut(s) 55, 58, 584, 684
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 2 cut(s) 11, 93
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 5 cut(s) 18, 265, 292, 400, 623
Mox20I TGGCCA 1 cut(s) 221
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 3 cut(s) 73, 191, 207
MscI TGGCCA 1 cut(s) 221
Msp20I TGGCCA 1 cut(s) 221
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 4 cut(s) 128, 159, 395, 614
NdeI CATATG 1 cut(s) 283
NdeII GATC 1 cut(s) 235
NlaIII CATG 2 cut(s) 327, 408
NlaIV GGNNCC 2 cut(s) 121, 139
NmuCI GTSAC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 1 cut(s) 408
PaeI GCATGC 1 cut(s) 408
PdmI GAANNNNTTC 3 cut(s) 73, 191, 207
PfeI GAWTC 4 cut(s) 214, 351, 371, 429
PkrI GCNGC 4 cut(s) 100, 610, 635, 638
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 121, 139
PstNI CAGNNNCTG 2 cut(s) 104, 217
SatI GCNGC 4 cut(s) 99, 609, 634, 637
Sau3AI GATC 1 cut(s) 235
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 458
SetI ASST 7 cut(s) 36, 76, 116, 270, 448, 486, 526
SfaNI GCATC 2 cut(s) 110, 154
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 408
Sse9I AATT 2 cut(s) 11, 93
SsiI CCGC 3 cut(s) 563, 608, 639
SspMI CTAG 3 cut(s) 176, 269, 447
StyD4I CCNGG 1 cut(s) 456
TaiI ACGT 1 cut(s) 526
TasI AATT 2 cut(s) 11, 93
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 4 cut(s) 214, 351, 371, 429
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 3 cut(s) 98, 633, 636
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 454, 459, 585
TspRI CASTG 1 cut(s) 174
XbaI TCTAGA 1 cut(s) 175
XceI RCATGY 1 cut(s) 408
XmnI GAANNNNTTC 3 cut(s) 73, 191, 207
XspI CTAG 3 cut(s) 176, 269, 447
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.