RchiOBHm_Chr4g0386711

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Reverse (-)
2142501 .. 2143548
1048 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36006

Sequence Viewer

Length: 438 bp
ATGGTTGTAAGGATTATATGCAGTCTTTTGATTTTATTCAAGTTCAACATATTTACCGTGAAGCAAATTGTGTCACCCATTAGATTAGTGCACTTTGCTGGTTATGATTATGTTATTCAGGAAAATTGTAGAATTGCAATCGAGGCACGTGAAATTTACCATGACAAATGCACCAAACGTTCTTGGGAAATTGTGAACACAGGAAAGCCTTCAAGACATCCAGTTCTATTTGCAGCATCTGTGGGAAGCTTTGGAGCCTATTTGGCTGATGGTTCTGAGTATAGGGAGATTCTAGCCAACTCTGTTGCTCATCTAATTGCATTTGAGACGACACTGAATAAGTTAGAGGCAAAGGCATGTGCTGAACTTCTTGATGAAGAAGCATTAGATTTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

145

Amino Acids

16.46

Weight (kDa)

6.19

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 178
AcsI RAATTY 1 cut(s) 153
AcvI CACGTG 1 cut(s) 149
AgsI TTSAA 3 cut(s) 40, 46, 213
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 320
Alw44I GTGCAC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 239
ApaLI GTGCAC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 66
BaeGI GKGCMC 1 cut(s) 93
BbrPI CACGTG 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 245
BccI CCATC 2 cut(s) 263, 415
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 256
BmsI GCATC 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 221
BseGI GGATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 221
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 320
BsmBI CGTCTC 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 256
BsrI ACTGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 58
BstBAI YACGTR 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 276
BstF5I GGATG 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 320
BstMWI GCNNNNNNNGC 2 cut(s) 143, 263
BstNSI RCATGY 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 245
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 2 cut(s) 332, 435
CaiI CAGNNNCTG 1 cut(s) 239
CviAII CATG 3 cut(s) 161, 357, 398
CviJI RGCY 5 cut(s) 208, 249, 257, 266, 296
CviKI_1 RGCY 5 cut(s) 208, 249, 257, 266, 296
DdeI CTNAG 1 cut(s) 276
Eco72I CACGTG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 3 cut(s) 164, 360, 401
FaiI YATR 9 cut(s) 17, 19, 50, 105, 111, 162, 282, 358, 399
FatI CATG 3 cut(s) 160, 356, 397
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 293
GluI GCNGC 1 cut(s) 234
Hin1II CATG 3 cut(s) 164, 360, 401
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 2 cut(s) 289, 425
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 91, 196, 404
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 3 cut(s) 119, 213, 371
Hpy8I GTNNAC 3 cut(s) 91, 196, 404
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 2 cut(s) 148, 178
HpyCH4V TGCA 6 cut(s) 21, 91, 137, 171, 233, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 263
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 2 cut(s) 148, 178
Hsp92II CATG 3 cut(s) 164, 360, 401
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 5 cut(s) 84, 104, 186, 234, 407
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 245
MaeI CTAG 1 cut(s) 293
MaeII ACGT 2 cut(s) 148, 178
MaeIII GTNAC 1 cut(s) 72
MboII GAAGA 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 6 cut(s) 66, 124, 132, 153, 189, 315
MnlI CCTC 2 cut(s) 136, 340
MroXI GAANNNNTTC 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 143, 263
NlaIII CATG 3 cut(s) 164, 360, 401
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 72
NspI RCATGY 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 289, 425
PkrI GCNGC 1 cut(s) 235
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 178
PspCI CACGTG 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 239
SatI GCNGC 1 cut(s) 234
SduI GDGCHC 1 cut(s) 93
SetI ASST 3 cut(s) 151, 181, 251
SfaNI GCATC 1 cut(s) 245
Sse9I AATT 6 cut(s) 66, 124, 132, 153, 189, 315
SspMI CTAG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 2 cut(s) 151, 181
TaqI TCGA 1 cut(s) 141
TasI AATT 6 cut(s) 66, 124, 132, 153, 189, 315
TfiI GAWTC 2 cut(s) 289, 425
TscAI CASTG 2 cut(s) 339, 435
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 72
TspDTI ATGAA 1 cut(s) 390
TspRI CASTG 2 cut(s) 339, 435
VneI GTGCAC 1 cut(s) 89
XapI RAATTY 1 cut(s) 153
XceI RCATGY 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 208
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.