FvH4_4g00800

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002729
Physical Location & Seq
Reverse (-)
672191 .. 675901
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00800.t2

Sequence Viewer

Length: 1041 bp
ATGGGTACTTTGGGAAGCGAGGTGAAGACATCGTCATCGTCGTTCATGAGTGATTTCCTTGAGAAGTGCGGTGGCTGTGCGGTTCTGGACGGCGGATTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCATCAGTTCTCCTCACCTCGTCCGAAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATTATAATAACCGCATCATATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTGTAGAGAAGAAGCCAAAGCTCTGCTGAGGAAAAGTGTAGAGATTGCAATTGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACACTGGAAAGCCTTTAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGTGGCAACTACGGTGATGCAGTGAGTCTAGAAACATTGAAAGATTTTCATAAGGAAAGGGTTCAGATTCTGGCCAATTCTGGTGCTGATCTAATTGCATTTGAGACGATACCGAATAAGCTAGAGGCAAAGGCATATGCTGAACTTCTTGAGGAAGAAGCAATTGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAATGTGGTGAGCGGTGATTCCATCTTTGAGTGTGCCTCTATTGCTAATGCATGCAAGCAAGTTGTTGCGGTTGGAATCAACTGCACACCACCTCGATTTATCCAGGGGCTGATTTCATCTATTCGGAAGATAACAAGCAAACCAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGCCAGAGCAAGCAATGGGTGCCATCGAGTGGGCGGGTTGATGAAGAGTTTGCAGACATAGTCGTAGAGAAGTGGTGTGAGGCCGGGGCTTCCCTGTTTGGAGGCTGCTGCAGAACAACTCCAAATACAATAAGAGCAATATCTAAGGCACTTTCTAACAAGCCTTCTTCAGCGGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

347

Amino Acids

37.28

Weight (kDa)

5.07

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 28 - 370 1.5e-78 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 877
AccB7I CCANNNNNTGG 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 690
AciI CCGC 8 cut(s) 69, 80, 93, 234, 690, 746, 892, 1031
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 2 cut(s) 543, 859
AcsI RAATTY 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 1011
AfaI GTAC 2 cut(s) 7, 192
AfiI CCNNNNNNNGG 2 cut(s) 842, 887
AgsI TTSAA 1 cut(s) 511
AjnI CCWGG 1 cut(s) 780
AluBI AGCT 5 cut(s) 109, 125, 299, 438, 592
AluI AGCT 5 cut(s) 109, 125, 299, 438, 592
Alw26I GTCTC 2 cut(s) 569, 842
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 3 cut(s) 428, 541, 787
AoxI GGCC 5 cut(s) 246, 267, 543, 859, 939
ApeKI GCWGC 3 cut(s) 422, 963, 966
ApoI RAATTY 1 cut(s) 342
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 943
AsuHPI GGTGA 6 cut(s) 34, 167, 497, 697, 704, 857
BalI TGGCCA 2 cut(s) 545, 861
BanI GGYRCC 1 cut(s) 877
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 3 cut(s) 434, 950, 953
BccI CCATC 5 cut(s) 452, 665, 707, 851, 889
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 782
BcnI CCSGG 1 cut(s) 943
BcoDI GTCTC 2 cut(s) 569, 842
BfaI CTAG 3 cut(s) 416, 500, 593
BfmI CTRYAG 2 cut(s) 280, 967
BfoI RGCGCY 1 cut(s) 153
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 3 cut(s) 423, 964, 967
BlsI GCNGC 3 cut(s) 424, 965, 968
Bme1390I CCNGG 2 cut(s) 782, 943
BmiI GGNNCC 3 cut(s) 445, 463, 879
BmrFI CCNGG 2 cut(s) 782, 943
BmsI GCATC 5 cut(s) 199, 245, 434, 478, 1024
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 4 cut(s) 114, 146, 698, 730
BpmI CTGGAG 2 cut(s) 166, 234
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 80, 641
BpuMI CCSGG 1 cut(s) 943
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 2 cut(s) 781, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 842, 887
Bse1I ACTGG 2 cut(s) 394, 410
Bse3DI GCAATG 1 cut(s) 878
BseBI CCWGG 1 cut(s) 782
BseDI CCNNGG 2 cut(s) 781, 942
BseGI GGATG 2 cut(s) 406, 1039
BseLI CCNNNNNNNGG 2 cut(s) 842, 887
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 394, 410
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 434, 950, 953
BsgI GTGCAG 1 cut(s) 745
BshFI GGCC 5 cut(s) 248, 269, 545, 861, 941
BshNI GGYRCC 1 cut(s) 877
BsiSI CCGG 1 cut(s) 942
BslI CCNNNNNNNGG 2 cut(s) 842, 887
BsmAI GTCTC 2 cut(s) 569, 842
BsmBI CGTCTC 1 cut(s) 569
BsnI GGCC 5 cut(s) 248, 269, 545, 861, 941
Bso31I GGTCTC 1 cut(s) 842
Bsp143I GATC 3 cut(s) 127, 136, 559
BspACI CCGC 8 cut(s) 69, 80, 93, 234, 690, 746, 892, 1031
BspANI GGCC 5 cut(s) 248, 269, 545, 861, 941
BspCNI CTCAG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 445, 463, 879
BspMAI CTGCAG 1 cut(s) 971
BspPI GGATC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 877
BspTNI GGTCTC 1 cut(s) 842
BsrBI CCGCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 878
BsrI ACTGG 2 cut(s) 394, 410
BssECI CCNNGG 2 cut(s) 781, 942
BssMI GATC 3 cut(s) 127, 136, 559
BssSI CACGAG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 485, 842
Bst6I CTCTTC 1 cut(s) 897
BstAPI GCANNNNNTGC 1 cut(s) 877
BstC8I GCNNGC 3 cut(s) 730, 734, 869
BstDEI CTNAG 3 cut(s) 305, 1002, 1038
BstF5I GGATG 2 cut(s) 406, 1039
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 130, 139, 562
BstMAI GTCTC 2 cut(s) 569, 842
BstMBI GATC 3 cut(s) 127, 136, 559
BstMWI GCNNNNNNNGC 5 cut(s) 156, 332, 452, 719, 877
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 780, 941
BstSFI CTRYAG 2 cut(s) 280, 967
BstV1I GCAGC 3 cut(s) 434, 950, 953
BstV2I GAAGAC 1 cut(s) 32
BsuRI GGCC 5 cut(s) 248, 269, 545, 861, 941
BtsCI GGATG 2 cut(s) 406, 1039
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 3 cut(s) 387, 498, 847
Cac8I GCNNGC 3 cut(s) 730, 734, 869
CaiI CAGNNNCTG 3 cut(s) 428, 541, 787
CciI TCATGA 1 cut(s) 45
CfoI GCGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 6, 191
CviAII CATG 4 cut(s) 46, 119, 648, 729
CviQI GTAC 2 cut(s) 6, 191
DdeI CTNAG 3 cut(s) 305, 1002, 1038
DpnI GATC 3 cut(s) 129, 138, 561
DpnII GATC 3 cut(s) 127, 136, 559
EaeI YGGCCR 2 cut(s) 543, 859
Eam1104I CTCTTC 1 cut(s) 897
EarI CTCTTC 1 cut(s) 897
EciI GGCGGA 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 842
Eco57I CTGAAG 1 cut(s) 1011
EcoRII CCWGG 1 cut(s) 780
EcoT22I ATGCAT 1 cut(s) 730
Esp3I CGTCTC 1 cut(s) 569
FaeI CATG 4 cut(s) 49, 122, 651, 732
FatI CATG 4 cut(s) 45, 118, 647, 728
FauI CCCGC 1 cut(s) 885
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 423, 964, 967
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 423, 964, 967
FspBI CTAG 3 cut(s) 416, 500, 593
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 423, 964, 967
GsuI CTGGAG 2 cut(s) 166, 234
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 5 cut(s) 248, 269, 545, 861, 941
HapII CCGG 1 cut(s) 942
HhaI GCGC 1 cut(s) 152
Hin1II CATG 4 cut(s) 49, 122, 651, 732
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 5 cut(s) 496, 538, 675, 695, 753
HpaII CCGG 1 cut(s) 942
HphI GGTGA 6 cut(s) 34, 167, 497, 697, 704, 857
Hpy166II GTNNAC 2 cut(s) 385, 654
Hpy188I TCNGA 4 cut(s) 185, 466, 537, 804
Hpy188III TCNNGA 5 cut(s) 46, 86, 145, 500, 620
Hpy8I GTNNAC 2 cut(s) 385, 654
Hpy99I CGWCG 2 cut(s) 43, 106
HpyAV CCTTC 2 cut(s) 180, 1032
HpyCH4III ACNGT 2 cut(s) 485, 842
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 332, 452, 719, 877
HpyF3I CTNAG 3 cut(s) 305, 1002, 1038
Hsp92II CATG 4 cut(s) 49, 122, 651, 732
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 127, 136, 559
LmnI GCTCC 5 cut(s) 106, 122, 147, 215, 443
Lsp1109I GCAGC 3 cut(s) 434, 950, 953
LweI GCATC 5 cut(s) 199, 245, 434, 478, 1024
MaeI CTAG 3 cut(s) 416, 500, 593
MalI GATC 3 cut(s) 129, 138, 561
MbiI CCGCTC 1 cut(s) 690
MboI GATC 3 cut(s) 127, 136, 559
MboII GAAGA 6 cut(s) 37, 299, 638, 817, 914, 1017
MfeI CAATTG 2 cut(s) 327, 633
MlsI TGGCCA 2 cut(s) 545, 861
MluCI AATT 6 cut(s) 252, 327, 342, 547, 564, 633
MluNI TGGCCA 2 cut(s) 545, 861
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 730
Mox20I TGGCCA 2 cut(s) 545, 861
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 531
MscI TGGCCA 2 cut(s) 545, 861
MseI TTAA 1 cut(s) 401
Msp20I TGGCCA 2 cut(s) 545, 861
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 2 cut(s) 782, 943
MunI CAATTG 2 cut(s) 327, 633
MvaI CCWGG 1 cut(s) 782
MwoI GCNNNNNNNGC 5 cut(s) 156, 332, 452, 719, 877
NciI CCSGG 1 cut(s) 943
NdeI CATATG 1 cut(s) 607
NdeII GATC 3 cut(s) 127, 136, 559
NlaIII CATG 4 cut(s) 49, 122, 651, 732
NlaIV GGNNCC 3 cut(s) 445, 463, 879
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 732
PaeI GCATGC 1 cut(s) 732
PagI TCATGA 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 4 cut(s) 538, 675, 695, 753
PflFI GACNNNGTC 2 cut(s) 31, 917
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 3 cut(s) 424, 965, 968
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 780
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 445, 463, 879
PstI CTGCAG 1 cut(s) 971
PstNI CAGNNNCTG 3 cut(s) 428, 541, 787
PsyI GACNNNGTC 2 cut(s) 31, 917
RsaI GTAC 2 cut(s) 7, 192
RsaNI GTAC 2 cut(s) 6, 191
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 3 cut(s) 423, 964, 967
Sau3AI GATC 3 cut(s) 127, 136, 559
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 2 cut(s) 782, 943
SfaNI GCATC 5 cut(s) 199, 245, 434, 478, 1024
SfcI CTRYAG 2 cut(s) 280, 967
SmlI CTYRAG 2 cut(s) 59, 620
SmoI CTYRAG 2 cut(s) 59, 620
SphI GCATGC 1 cut(s) 732
Sse9I AATT 6 cut(s) 252, 327, 342, 547, 564, 633
SsiI CCGC 8 cut(s) 69, 80, 93, 234, 690, 746, 892, 1031
SspMI CTAG 3 cut(s) 416, 500, 593
StyD4I CCNGG 2 cut(s) 780, 941
TaaI ACNGT 2 cut(s) 485, 842
TaqI TCGA 2 cut(s) 772, 884
TasI AATT 6 cut(s) 252, 327, 342, 547, 564, 633
TfiI GAWTC 4 cut(s) 538, 675, 695, 753
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 3 cut(s) 394, 498, 847
TseI GCWGC 3 cut(s) 422, 963, 966
TspDTI ATGAA 4 cut(s) 34, 509, 783, 915
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 3 cut(s) 394, 498, 847
Tth111I GACNNNGTC 2 cut(s) 31, 917
Van91I CCANNNNNTGG 1 cut(s) 887
XapI RAATTY 1 cut(s) 342
XbaI TCTAGA 1 cut(s) 499
XceI RCATGY 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 3 cut(s) 416, 500, 593
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.