RchiOBHm_Chr4g0387351

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Reverse (-)
2501624 .. 2504549
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36067

Sequence Viewer

Length: 1065 bp
ATGGATTTGGGAAGCGAGAAAACGTCGTCGTTCATGAGCGATTTTCTGGAAAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGACCCTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGTAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACTATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAAGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAATGTACCAAAGGTTCTTGGGAGTTTGTGGACACAGGAAAGCCATCAAGACATCCAGTTCTAGTTGCAGCATCTGTAGGAAGCTATGGAGCCTATTTGGCTGACGGTTCCGAGTATAGTGGCAACTACGGCGATGCAGTGACTCTAGAAACGTTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGGTGCTGATCTAATTGCATTTGAGACGACACCGAATAAGCTAGAGGCCAAGGCATATGCTGAACTTCTTGAGGAAGAAGCAATAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAATGTGGTGAGTGGTGATTCCATCTTTGAGTGTTCCTCTATTGCTAATGCATGCAAGCAAGTTGTTGCTGTTGGAATCAACTGCACACCACCTAGATTTATCCAGGGGTTGATTTCATCTATTCGGAAGGTAACAAGCAAACCAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGTCAGAGCAAGCAATGGATTGTAAGTGTCCTCCTATATGAATACACAGTGCTTCCATCCAGTGGGCGGGTTGATGAAGAGTTTGCAGAAATAGTCATAGAGAAGTGGCGTGAGGCAGGGGCTTCCCTGTTTGGAGGCTGCTGCAGAACAACTCCAAATACCATAAGGGCAATATCTAAGGCTCTTTCCAACAAGTCTTCTGCGGTTAACTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

354

Amino Acids

38.47

Weight (kDa)

5.1

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 344 5.1e-80 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 908
AciI CCGC 5 cut(s) 57, 81, 222, 913, 1049
AclI AACGTT 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 2 cut(s) 180, 349
AfiI CCNNNNNNNGG 4 cut(s) 294, 830, 908, 912
AgsI TTSAA 1 cut(s) 499
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 4 cut(s) 97, 113, 426, 580
AluI AGCT 4 cut(s) 97, 113, 426, 580
Alw26I GTCTC 2 cut(s) 557, 830
AlwNI CAGNNNCTG 2 cut(s) 416, 529
AoxI GGCC 4 cut(s) 234, 255, 531, 585
ApeKI GCWGC 3 cut(s) 410, 984, 987
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 135, 167
Asp700I GAANNNNTTC 2 cut(s) 503, 519
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 4 cut(s) 155, 685, 692, 845
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BalI TGGCCA 1 cut(s) 533
BauI CACGAG 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 1035
BbvI GCAGC 3 cut(s) 422, 971, 974
BccI CCATC 5 cut(s) 394, 653, 695, 839, 910
BceAI ACGGC 3 cut(s) 53, 94, 487
BciT130I CCWGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 557, 830
BfaI CTAG 4 cut(s) 404, 488, 581, 759
BfmI CTRYAG 2 cut(s) 417, 988
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 411, 985, 988
BlsI GCNGC 3 cut(s) 412, 986, 989
Bme1390I CCNGG 1 cut(s) 770
BmiI GGNNCC 2 cut(s) 433, 451
BmrFI CCNGG 1 cut(s) 770
BmsI GCATC 4 cut(s) 187, 233, 422, 466
BpiI GAAGAC 1 cut(s) 1035
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 686, 718
BpmI CTGGAG 2 cut(s) 154, 222
Bpu10I CCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 588, 769
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 830, 908, 912
Bse1I ACTGG 2 cut(s) 398, 906
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 2 cut(s) 588, 769
BseGI GGATG 2 cut(s) 394, 902
BseLI CCNNNNNNNGG 4 cut(s) 294, 830, 908, 912
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 1 cut(s) 307
BseNI ACTGG 2 cut(s) 398, 906
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 3 cut(s) 422, 971, 974
BsgI GTGCAG 1 cut(s) 733
BshFI GGCC 4 cut(s) 236, 257, 533, 587
BslI CCNNNNNNNGG 4 cut(s) 294, 830, 908, 912
BsmAI GTCTC 2 cut(s) 557, 830
BsmBI CGTCTC 1 cut(s) 557
BsnI GGCC 4 cut(s) 236, 257, 533, 587
Bso31I GGTCTC 1 cut(s) 830
Bsp143I GATC 2 cut(s) 115, 547
BspACI CCGC 5 cut(s) 57, 81, 222, 913, 1049
BspANI GGCC 4 cut(s) 236, 257, 533, 587
BspCNI CTCAG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 433, 451
BspMAI CTGCAG 1 cut(s) 992
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 866
BsrI ACTGG 2 cut(s) 398, 906
BssECI CCNNGG 2 cut(s) 588, 769
BssMI GATC 2 cut(s) 115, 547
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 3 cut(s) 449, 830, 895
Bst6I CTCTTC 1 cut(s) 918
BstC8I GCNNGC 3 cut(s) 718, 722, 857
BstDEI CTNAG 3 cut(s) 293, 1023, 1062
BstF5I GGATG 2 cut(s) 394, 902
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 2 cut(s) 118, 550
BstMAI GTCTC 2 cut(s) 557, 830
BstMBI GATC 2 cut(s) 115, 547
BstMWI GCNNNNNNNGC 4 cut(s) 144, 320, 440, 471
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 2 cut(s) 417, 988
BstV1I GCAGC 3 cut(s) 422, 971, 974
BstV2I GAAGAC 1 cut(s) 1035
BsuRI GGCC 4 cut(s) 236, 257, 533, 587
BtgZI GCGATG 1 cut(s) 489
BtsCI GGATG 2 cut(s) 394, 902
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 4 cut(s) 486, 835, 900, 913
Cac8I GCNNGC 3 cut(s) 718, 722, 857
CaiI CAGNNNCTG 2 cut(s) 416, 529
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 179, 348
CviAII CATG 4 cut(s) 34, 107, 636, 717
CviQI GTAC 2 cut(s) 179, 348
DdeI CTNAG 3 cut(s) 293, 1023, 1062
DpnI GATC 2 cut(s) 117, 549
DpnII GATC 2 cut(s) 115, 547
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 588
Eco31I GGTCTC 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 588
EcoT22I ATGCAT 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 4 cut(s) 37, 110, 639, 720
FatI CATG 4 cut(s) 33, 106, 635, 716
FauI CCCGC 2 cut(s) 74, 906
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 3 cut(s) 411, 985, 988
FokI GGATG 2 cut(s) 381, 889
Fsp4HI GCNGC 3 cut(s) 411, 985, 988
FspBI CTAG 4 cut(s) 404, 488, 581, 759
GlaI GCGC 1 cut(s) 139
GluI GCNGC 3 cut(s) 411, 985, 988
GsuI CTGGAG 2 cut(s) 154, 222
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 4 cut(s) 236, 257, 533, 587
HhaI GCGC 1 cut(s) 140
Hin1II CATG 4 cut(s) 37, 110, 639, 720
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HinfI GANTC 5 cut(s) 484, 526, 663, 683, 741
HpaI GTTAAC 1 cut(s) 1054
HphI GGTGA 4 cut(s) 155, 685, 692, 845
Hpy166II GTNNAC 3 cut(s) 373, 642, 1054
Hpy188I TCNGA 4 cut(s) 454, 525, 792, 852
Hpy188III TCNNGA 8 cut(s) 34, 47, 74, 133, 295, 390, 488, 608
Hpy8I GTNNAC 3 cut(s) 373, 642, 1054
Hpy99I CGWCG 3 cut(s) 28, 31, 94
HpyAV CCTTC 1 cut(s) 787
HpyCH4III ACNGT 3 cut(s) 449, 830, 895
HpyCH4IV ACGT 2 cut(s) 23, 494
HpyCH4V TGCA 9 cut(s) 314, 410, 479, 557, 716, 720, 750, 932, 990
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 320, 440, 471
HpyF3I CTNAG 3 cut(s) 293, 1023, 1062
HpySE526I ACGT 2 cut(s) 23, 494
Hsp92II CATG 4 cut(s) 37, 110, 639, 720
HspAI GCGC 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 1054
Kzo9I GATC 2 cut(s) 115, 547
LmnI GCTCC 5 cut(s) 94, 110, 135, 203, 431
Lsp1109I GCAGC 3 cut(s) 422, 971, 974
LweI GCATC 4 cut(s) 187, 233, 422, 466
MaeI CTAG 4 cut(s) 404, 488, 581, 759
MaeII ACGT 2 cut(s) 23, 494
MaeIII GTNAC 2 cut(s) 481, 796
MalI GATC 2 cut(s) 117, 549
MboI GATC 2 cut(s) 115, 547
MboII GAAGA 4 cut(s) 287, 626, 935, 1035
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 3 cut(s) 309, 330, 552
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 2 cut(s) 718, 1059
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 2 cut(s) 503, 519
MscI TGGCCA 1 cut(s) 533
MseI TTAA 1 cut(s) 1053
Msp20I TGGCCA 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 770
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 4 cut(s) 144, 320, 440, 471
NdeI CATATG 1 cut(s) 595
NdeII GATC 2 cut(s) 115, 547
NlaIII CATG 4 cut(s) 37, 110, 639, 720
NlaIV GGNNCC 2 cut(s) 433, 451
NmuCI GTSAC 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 720
PaeI GCATGC 1 cut(s) 720
PagI TCATGA 1 cut(s) 33
PdmI GAANNNNTTC 2 cut(s) 503, 519
PfeI GAWTC 4 cut(s) 526, 663, 683, 741
PflMI CCANNNNNTGG 1 cut(s) 908
PkrI GCNGC 3 cut(s) 412, 986, 989
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp1406I AACGTT 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 768
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 2 cut(s) 433, 451
PsrI GAACNNNNNNTAC 2 cut(s) 340, 372
PstI CTGCAG 1 cut(s) 992
PstNI CAGNNNCTG 2 cut(s) 416, 529
RsaI GTAC 2 cut(s) 180, 349
RsaNI GTAC 2 cut(s) 179, 348
SaqAI TTAA 1 cut(s) 1053
SatI GCNGC 3 cut(s) 411, 985, 988
Sau3AI GATC 2 cut(s) 115, 547
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 770
SfaNI GCATC 4 cut(s) 187, 233, 422, 466
SfcI CTRYAG 2 cut(s) 417, 988
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SphI GCATGC 1 cut(s) 720
Sse9I AATT 3 cut(s) 309, 330, 552
SsiI CCGC 5 cut(s) 57, 81, 222, 913, 1049
SspMI CTAG 4 cut(s) 404, 488, 581, 759
StyD4I CCNGG 1 cut(s) 768
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 3 cut(s) 449, 830, 895
TaiI ACGT 2 cut(s) 26, 497
TaqI TCGA 1 cut(s) 318
TasI AATT 3 cut(s) 309, 330, 552
TfiI GAWTC 4 cut(s) 526, 663, 683, 741
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 4 cut(s) 486, 835, 900, 913
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 3 cut(s) 410, 984, 987
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 4 cut(s) 22, 771, 900, 936
TspGWI ACGGA 2 cut(s) 107, 161
TspRI CASTG 4 cut(s) 486, 835, 900, 913
Van91I CCANNNNNTGG 1 cut(s) 908
XapI RAATTY 1 cut(s) 330
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 503, 519
XspI CTAG 4 cut(s) 404, 488, 581, 759
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.