Rroxscaffold_5G00334070

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002729
Physical Location & Seq
Reverse (-)
1404130 .. 1407306
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334070.1

Sequence Viewer

Length: 1032 bp
ATGGATTTGGGAAGCGAGAAAACGTCGTCGTTCATGAGCGATTTTCTGGAAAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGACCCTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGTAGGGTACATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGAGGCCAAAGGTTTCTCTAAGGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGGTTCCGAGTATAGTGGCAACTATGGCGATGCAGTAACTCTAGAAACATTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGGTGCTGATCTAATTGCATTTGAGACAACTCCGAATAAGCTAGAGGCGAAGGCATATACTGCACTTCTTGAGGAAGAAGCAATAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAATGTGGTGAGTGGTGATTCCATCTTTGAGTGTGCCTCTATTGCTAATGCATGCAAGCAAGTTGTTGCTGTTGGAATCAACTGCACACCACCTAGATTTATCCAGGGGTTGATTTCATCTATTCGGAAGGTAACAAGCAAACCAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGTCAGAGCAAGCAATGGGTGCCATCGAGTGGGAGGGTTGATGAAGAGTTTGCAGAAATAGTCATAGCGAAGTGGCGTGAGGCTGGGGCTTCCCTGTTTGGAGGCTGCTGCAGAACAACTCCAAATACCATAAGGGCAATATCTAAGGCTCTTTCTAATAAGTCTTCTGCAGTTAACGACGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

343

Amino Acids

37.01

Weight (kDa)

5.22

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 333 2.3e-81 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 865
AccB7I CCANNNNNTGG 1 cut(s) 875
AciI CCGC 3 cut(s) 57, 81, 222
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 3 cut(s) 294, 830, 875
AgsI TTSAA 2 cut(s) 390, 499
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 4 cut(s) 97, 113, 426, 580
AluI AGCT 4 cut(s) 97, 113, 426, 580
Alw26I GTCTC 2 cut(s) 557, 830
AlwNI CAGNNNCTG 2 cut(s) 416, 529
AoxI GGCC 3 cut(s) 234, 255, 531
ApeKI GCWGC 3 cut(s) 410, 951, 954
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 135, 167
Asp700I GAANNNNTTC 3 cut(s) 385, 503, 519
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 4 cut(s) 155, 685, 692, 845
BalI TGGCCA 1 cut(s) 533
BanI GGYRCC 1 cut(s) 865
BauI CACGAG 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 1002
BbvI GCAGC 3 cut(s) 422, 938, 941
BccI CCATC 5 cut(s) 440, 653, 695, 839, 877
BceAI ACGGC 2 cut(s) 53, 94
BciT130I CCWGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 557, 830
BfaI CTAG 4 cut(s) 404, 488, 581, 759
BfmI CTRYAG 2 cut(s) 955, 1014
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 411, 952, 955
BlsI GCNGC 3 cut(s) 412, 953, 956
Bme1390I CCNGG 1 cut(s) 770
BmiI GGNNCC 3 cut(s) 433, 451, 867
BmrFI CCNGG 1 cut(s) 770
BmsI GCATC 4 cut(s) 187, 233, 422, 466
BpiI GAAGAC 1 cut(s) 1002
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 686, 718
BpmI CTGGAG 2 cut(s) 154, 222
Bpu10I CCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 1 cut(s) 769
Bsc4I CCNNNNNNNGG 3 cut(s) 294, 830, 875
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 1 cut(s) 769
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 3 cut(s) 294, 830, 875
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 1 cut(s) 307
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 3 cut(s) 422, 938, 941
BseYI CCCAGC 1 cut(s) 929
BsgI GTGCAG 2 cut(s) 585, 733
BshFI GGCC 3 cut(s) 236, 257, 533
BshNI GGYRCC 1 cut(s) 865
BslI CCNNNNNNNGG 3 cut(s) 294, 830, 875
BsmAI GTCTC 2 cut(s) 557, 830
BsnI GGCC 3 cut(s) 236, 257, 533
Bso31I GGTCTC 1 cut(s) 830
Bsp143I GATC 2 cut(s) 115, 547
BspACI CCGC 3 cut(s) 57, 81, 222
BspANI GGCC 3 cut(s) 236, 257, 533
BspCNI CTCAG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 433, 451, 867
BspMAI CTGCAG 2 cut(s) 959, 1018
BspT107I GGYRCC 1 cut(s) 865
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 866
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 769
BssMI GATC 2 cut(s) 115, 547
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 1 cut(s) 830
Bst6I CTCTTC 1 cut(s) 885
BstAPI GCANNNNNTGC 2 cut(s) 599, 865
BstC8I GCNNGC 3 cut(s) 718, 722, 857
BstDEI CTNAG 4 cut(s) 270, 293, 990, 1029
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 2 cut(s) 118, 550
BstMAI GTCTC 2 cut(s) 557, 830
BstMBI GATC 2 cut(s) 115, 547
BstMWI GCNNNNNNNGC 7 cut(s) 144, 320, 440, 471, 599, 707, 865
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 2 cut(s) 955, 1014
BstV1I GCAGC 3 cut(s) 422, 938, 941
BstV2I GAAGAC 1 cut(s) 1002
BsuRI GGCC 3 cut(s) 236, 257, 533
BtgZI GCGATG 1 cut(s) 489
BtsCI GGATG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 835
Cac8I GCNNGC 3 cut(s) 718, 722, 857
CaiI CAGNNNCTG 2 cut(s) 416, 529
CciI TCATGA 1 cut(s) 33
CfoI GCGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 179
CviAII CATG 4 cut(s) 34, 107, 636, 717
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 4 cut(s) 270, 293, 990, 1029
DpnI GATC 2 cut(s) 117, 549
DpnII GATC 2 cut(s) 115, 547
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
Eco31I GGTCTC 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 768
EcoT22I ATGCAT 1 cut(s) 718
FaeI CATG 4 cut(s) 37, 110, 639, 720
FatI CATG 4 cut(s) 33, 106, 635, 716
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 3 cut(s) 411, 952, 955
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 3 cut(s) 411, 952, 955
FspBI CTAG 4 cut(s) 404, 488, 581, 759
GlaI GCGC 1 cut(s) 139
GluI GCNGC 3 cut(s) 411, 952, 955
GsaI CCCAGC 1 cut(s) 933
GsuI CTGGAG 2 cut(s) 154, 222
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 3 cut(s) 236, 257, 533
HhaI GCGC 1 cut(s) 140
Hin1II CATG 4 cut(s) 37, 110, 639, 720
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 1021
HindII GTYRAC 1 cut(s) 1021
HinfI GANTC 4 cut(s) 526, 663, 683, 741
HpaI GTTAAC 1 cut(s) 1021
HphI GGTGA 4 cut(s) 155, 685, 692, 845
Hpy166II GTNNAC 3 cut(s) 373, 642, 1021
Hpy188I TCNGA 5 cut(s) 454, 525, 573, 792, 852
Hpy188III TCNNGA 8 cut(s) 34, 47, 74, 133, 295, 390, 488, 608
Hpy8I GTNNAC 3 cut(s) 373, 642, 1021
Hpy99I CGWCG 4 cut(s) 28, 31, 94, 1028
HpyAV CCTTC 3 cut(s) 396, 583, 787
HpyCH4III ACNGT 1 cut(s) 830
HpyCH4IV ACGT 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 7 cut(s) 144, 320, 440, 471, 599, 707, 865
HpyF3I CTNAG 4 cut(s) 270, 293, 990, 1029
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 4 cut(s) 37, 110, 639, 720
HspAI GCGC 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 2 cut(s) 115, 547
LmnI GCTCC 5 cut(s) 94, 110, 135, 203, 431
Lsp1109I GCAGC 3 cut(s) 422, 938, 941
LweI GCATC 4 cut(s) 187, 233, 422, 466
MaeI CTAG 4 cut(s) 404, 488, 581, 759
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 481, 796
MalI GATC 2 cut(s) 117, 549
MboI GATC 2 cut(s) 115, 547
MboII GAAGA 4 cut(s) 287, 626, 902, 1002
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 4 cut(s) 240, 309, 330, 552
MluNI TGGCCA 1 cut(s) 533
MmeI TCCRAC 1 cut(s) 718
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 3 cut(s) 385, 503, 519
MscI TGGCCA 1 cut(s) 533
MseI TTAA 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 770
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 7 cut(s) 144, 320, 440, 471, 599, 707, 865
NdeII GATC 2 cut(s) 115, 547
NlaIII CATG 4 cut(s) 37, 110, 639, 720
NlaIV GGNNCC 3 cut(s) 433, 451, 867
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 720
PaeI GCATGC 1 cut(s) 720
PagI TCATGA 1 cut(s) 33
PdmI GAANNNNTTC 3 cut(s) 385, 503, 519
PfeI GAWTC 4 cut(s) 526, 663, 683, 741
PflMI CCANNNNNTGG 1 cut(s) 875
PkrI GCNGC 3 cut(s) 412, 953, 956
Psp6I CCWGG 1 cut(s) 768
PspFI CCCAGC 1 cut(s) 929
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 3 cut(s) 433, 451, 867
PstI CTGCAG 2 cut(s) 959, 1018
PstNI CAGNNNCTG 2 cut(s) 416, 529
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 3 cut(s) 411, 952, 955
Sau3AI GATC 2 cut(s) 115, 547
ScrFI CCNGG 1 cut(s) 770
SfaNI GCATC 4 cut(s) 187, 233, 422, 466
SfcI CTRYAG 2 cut(s) 955, 1014
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SphI GCATGC 1 cut(s) 720
Sse9I AATT 4 cut(s) 240, 309, 330, 552
SsiI CCGC 3 cut(s) 57, 81, 222
SspMI CTAG 4 cut(s) 404, 488, 581, 759
StyD4I CCNGG 1 cut(s) 768
TaaI ACNGT 1 cut(s) 830
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 318, 872
TasI AATT 4 cut(s) 240, 309, 330, 552
TfiI GAWTC 4 cut(s) 526, 663, 683, 741
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 1 cut(s) 835
TseI GCWGC 3 cut(s) 410, 951, 954
TspDTI ATGAA 3 cut(s) 22, 771, 903
TspGWI ACGGA 2 cut(s) 107, 161
TspRI CASTG 1 cut(s) 835
Van91I CCANNNNNTGG 1 cut(s) 875
XapI RAATTY 1 cut(s) 330
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 720
XmnI GAANNNNTTC 3 cut(s) 385, 503, 519
XspI CTAG 4 cut(s) 404, 488, 581, 759
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.