RchiOBHm_Chr4g0386961

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Reverse (-)
2271943 .. 2274349
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36030

Sequence Viewer

Length: 1032 bp
ATGGGTTTGGGAAGCGAGGTGAAAACGTCGTCGTTCAAGAGCGATTTTCTGGAGAAGTGCAGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAATGATCCTCTCTGGAGTGCCAAATGCCTCGTCAGTTCTCACCTCGTCCGAAGGGTACATTTAGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACAATTCAGGGTTTTGGGGCCAAAGGTTTCTCTAAAGAAGAGGCCAAACCTCTGGTCAGGAAAAGTGTAGAAATTGCAATCGAGGCACGAGAAATTTACTATGACAAATGCACCAAAGGTTCTTGGGCTTTTGTGGACACAGGAAAGCCTTCAAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGAAGCCTATTTGGCTGATGGTTCCGAGTATAGTGGCAACTATGGCGATGCAGTGACTCTAGAAACGTTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGGTGCTGATCTAATTGCATTTGTGACGACACCGAATAAGCTAGAGGCCAAGGCATATGCTGAACTTCTTGAGGAAGAAGCAATAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGGAATCAATGTGGTGAGTGGTGATTCCATCTTTGAGTGTGCCTCTATTGCTAATGCATGCAAGCAAGTTGTTGCTGTTGGAATCAACTGCACACCACCTAGATTTATCCAGGGGTTGATTTCATCTATTCGGAAGGTAACAAGCAAACCAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGTCAGAGCAAGCAATGGATTCCATCGAGTGTGCGGGTTGATGAAGAGTTTGCAGAAATAGTCATAGAGAAGTGGCGTGAGGCAGGGGCTTCTCTGTTTGGAGGCTGCTGCAGAATAACTCCAAATACCATAAGGGCAATATCTAAGGCTCTTTCTAACAAGTCTTTTGCGGTTAACATCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

343

Amino Acids

37.13

Weight (kDa)

5.55

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 333 1.7e-77 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 84, 222, 880, 1016
AclI AACGTT 1 cut(s) 494
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 830
AgsI TTSAA 3 cut(s) 37, 390, 499
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 4 cut(s) 100, 116, 426, 580
AluI AGCT 4 cut(s) 100, 116, 426, 580
Alw26I GTCTC 1 cut(s) 830
AlwI GGATC 1 cut(s) 122
AlwNI CAGNNNCTG 3 cut(s) 66, 416, 529
AoxI GGCC 5 cut(s) 234, 255, 279, 531, 585
ApeKI GCWGC 3 cut(s) 410, 951, 954
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 6 cut(s) 71, 103, 138, 170, 276, 308
Asp700I GAANNNNTTC 3 cut(s) 385, 503, 519
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 5 cut(s) 31, 155, 685, 692, 845
BalI TGGCCA 1 cut(s) 533
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 3 cut(s) 422, 938, 941
BccI CCATC 5 cut(s) 440, 653, 695, 839, 877
BceAI ACGGC 2 cut(s) 56, 97
BciT130I CCWGG 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 830
BfaI CTAG 4 cut(s) 404, 488, 581, 759
BfmI CTRYAG 1 cut(s) 955
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 411, 952, 955
BlsI GCNGC 3 cut(s) 412, 953, 956
Bme1390I CCNGG 1 cut(s) 770
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 256, 451
BmrFI CCNGG 1 cut(s) 770
BmsI GCATC 4 cut(s) 187, 233, 422, 466
BplI GAGNNNNNCTC 4 cut(s) 105, 137, 686, 718
BpmI CTGGAG 3 cut(s) 71, 157, 222
BpuEI CTTGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 588, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 830
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 2 cut(s) 588, 769
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 1 cut(s) 830
BseMI GCAATG 1 cut(s) 866
BseNI ACTGG 1 cut(s) 398
BseXI GCAGC 3 cut(s) 422, 938, 941
BsgI GTGCAG 2 cut(s) 79, 733
BshFI GGCC 5 cut(s) 236, 257, 281, 533, 587
BslI CCNNNNNNNGG 1 cut(s) 830
BsmAI GTCTC 1 cut(s) 830
BsnI GGCC 5 cut(s) 236, 257, 281, 533, 587
Bso31I GGTCTC 1 cut(s) 830
Bsp143I GATC 3 cut(s) 118, 127, 547
BspACI CCGC 4 cut(s) 84, 222, 880, 1016
BspANI GGCC 5 cut(s) 236, 257, 281, 533, 587
BspLI GGNNCC 2 cut(s) 256, 451
BspMAI CTGCAG 1 cut(s) 959
BspPI GGATC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 866
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 2 cut(s) 588, 769
BssMI GATC 3 cut(s) 118, 127, 547
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 1 cut(s) 830
Bst6I CTCTTC 2 cut(s) 270, 885
BstC8I GCNNGC 3 cut(s) 718, 722, 857
BstDEI CTNAG 2 cut(s) 990, 1029
BstF5I GGATG 1 cut(s) 394
BstKTI GATC 3 cut(s) 121, 130, 550
BstMAI GTCTC 1 cut(s) 830
BstMBI GATC 3 cut(s) 118, 127, 547
BstMWI GCNNNNNNNGC 4 cut(s) 320, 440, 471, 707
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 768
BstSFI CTRYAG 1 cut(s) 955
BstV1I GCAGC 3 cut(s) 422, 938, 941
BstXI CCANNNNNNTGG 1 cut(s) 289
BsuRI GGCC 5 cut(s) 236, 257, 281, 533, 587
BtgZI GCGATG 2 cut(s) 489, 1009
BtsCI GGATG 1 cut(s) 394
BtsI GCAGTG 2 cut(s) 67, 486
BtsIMutI CAGTG 3 cut(s) 67, 486, 835
Cac8I GCNNGC 3 cut(s) 718, 722, 857
CaiI CAGNNNCTG 3 cut(s) 66, 416, 529
Cfr13I GGNCC 1 cut(s) 255
Csp6I GTAC 1 cut(s) 179
CviAII CATG 3 cut(s) 110, 636, 717
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 990, 1029
DpnI GATC 3 cut(s) 120, 129, 549
DpnII GATC 3 cut(s) 118, 127, 547
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 2 cut(s) 270, 885
EarI CTCTTC 2 cut(s) 270, 885
Eco130I CCWWGG 1 cut(s) 588
Eco31I GGTCTC 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 588
EcoT22I ATGCAT 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 3 cut(s) 113, 639, 720
FatI CATG 3 cut(s) 109, 635, 716
FauI CCCGC 2 cut(s) 77, 873
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 3 cut(s) 411, 952, 955
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 3 cut(s) 411, 952, 955
FspBI CTAG 4 cut(s) 404, 488, 581, 759
GluI GCNGC 3 cut(s) 411, 952, 955
GsuI CTGGAG 3 cut(s) 71, 157, 222
HaeIII GGCC 5 cut(s) 236, 257, 281, 533, 587
Hin1II CATG 3 cut(s) 113, 639, 720
HincII GTYRAC 1 cut(s) 1021
HindII GTYRAC 1 cut(s) 1021
HinfI GANTC 6 cut(s) 484, 526, 663, 683, 741, 865
HpaI GTTAAC 1 cut(s) 1021
HphI GGTGA 5 cut(s) 31, 155, 685, 692, 845
Hpy166II GTNNAC 3 cut(s) 373, 642, 1021
Hpy188I TCNGA 5 cut(s) 173, 454, 525, 792, 852
Hpy188III TCNNGA 8 cut(s) 37, 50, 77, 136, 295, 390, 488, 608
Hpy8I GTNNAC 3 cut(s) 373, 642, 1021
Hpy99I CGWCG 3 cut(s) 31, 34, 97
HpyAV CCTTC 3 cut(s) 168, 396, 787
HpyCH4III ACNGT 1 cut(s) 830
HpyCH4IV ACGT 2 cut(s) 26, 494
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 440, 471, 707
HpyF3I CTNAG 2 cut(s) 990, 1029
HpySE526I ACGT 2 cut(s) 26, 494
Hsp92II CATG 3 cut(s) 113, 639, 720
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 3 cut(s) 118, 127, 547
LmnI GCTCC 3 cut(s) 97, 113, 203
Lsp1109I GCAGC 3 cut(s) 422, 938, 941
LweI GCATC 4 cut(s) 187, 233, 422, 466
MaeI CTAG 4 cut(s) 404, 488, 581, 759
MaeII ACGT 2 cut(s) 26, 494
MaeIII GTNAC 3 cut(s) 481, 562, 796
MalI GATC 3 cut(s) 120, 129, 549
MboI GATC 3 cut(s) 118, 127, 547
MboII GAAGA 3 cut(s) 287, 626, 902
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 4 cut(s) 240, 309, 330, 552
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 718
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 3 cut(s) 385, 503, 519
MscI TGGCCA 1 cut(s) 533
MseI TTAA 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 770
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 4 cut(s) 320, 440, 471, 707
NdeI CATATG 1 cut(s) 595
NdeII GATC 3 cut(s) 118, 127, 547
NlaIII CATG 3 cut(s) 113, 639, 720
NlaIV GGNNCC 2 cut(s) 256, 451
NmuCI GTSAC 2 cut(s) 481, 562
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 720
PaeI GCATGC 1 cut(s) 720
PdmI GAANNNNTTC 3 cut(s) 385, 503, 519
PfeI GAWTC 5 cut(s) 526, 663, 683, 741, 865
PkrI GCNGC 3 cut(s) 412, 953, 956
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp1406I AACGTT 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 768
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 2 cut(s) 256, 451
PspPI GGNCC 1 cut(s) 255
PstI CTGCAG 1 cut(s) 959
PstNI CAGNNNCTG 3 cut(s) 66, 416, 529
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 3 cut(s) 411, 952, 955
Sau3AI GATC 3 cut(s) 118, 127, 547
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 770
SfaNI GCATC 4 cut(s) 187, 233, 422, 466
SfcI CTRYAG 1 cut(s) 955
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SphI GCATGC 1 cut(s) 720
Sse9I AATT 4 cut(s) 240, 309, 330, 552
SsiI CCGC 4 cut(s) 84, 222, 880, 1016
SspMI CTAG 4 cut(s) 404, 488, 581, 759
StyD4I CCNGG 1 cut(s) 768
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 830
TaiI ACGT 2 cut(s) 29, 497
TaqI TCGA 2 cut(s) 318, 872
TasI AATT 4 cut(s) 240, 309, 330, 552
TfiI GAWTC 5 cut(s) 526, 663, 683, 741, 865
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 3 cut(s) 67, 486, 835
TseFI GTSAC 2 cut(s) 481, 562
TseI GCWGC 3 cut(s) 410, 951, 954
Tsp45I GTSAC 2 cut(s) 481, 562
TspDTI ATGAA 3 cut(s) 444, 771, 903
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 3 cut(s) 67, 486, 835
XapI RAATTY 1 cut(s) 330
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 720
XmnI GAANNNNTTC 3 cut(s) 385, 503, 519
XspI CTAG 4 cut(s) 404, 488, 581, 759
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.