RchiOBHm_Chr4g0387331

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002729
Physical Location & Seq
Reverse (-)
2477186 .. 2480394
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36065

Sequence Viewer

Length: 1122 bp
ATGCCAAAGGAGGCTCTTTCTTTGTTTTTCGAGATGGTGGCTACTGGGATCAAACCCAATTCGGTGACTATGATGTGTATCATTTCTGCTTGCGCAAAGTTGAAGGATGTTGCTTTGAGTTGGTCTAGTGGGGTGGTTGATCAAGAGTTTGCAGCAGACGTAGCCATAGCCAATTGGCATTTCATACCTCACCTTCAAAACCGAACAGTTCCTGAGCAGCCAAAGTTTGTGTTCCCTATACTTTGTTTTAGTCCTACACTCTCCATGAGCGATTTTCTGGAGAAGTGCGGTGGCTGTGCCGTTCTGGACGGCGGGTTTGCGACGGAGCTGGAACGACATGGAGCTGATCTCAACGACCTTCTCTGGAGCGCCAAATGCCTCGTCAGTTCTCCTCACCTCGTCCGAAGGGTGCATTTGGACTACCTTGATGCTGGAGCAAACATCATAATAACCGCATCTTATCAGGCCACTATTCAGGGTTTTGAGGCCAAAGGTTTCTCCAAGGAAGAAGCCAAAGCCCTGCTCAGGAAAAGTGTAGAAATTGCAATCGAGGCAAGAGAAATTTATTATGACAAATGCACCAAAGGTTCTTGGGATTTTGTGGATACAGGAAAGCCATCGAGACATCCAGTTCTAGTTGCAGCATCTGTGGGAAGCTATGGAGCCTATTTGGCTGATGTAACTCTAGAAACATTGAAAGATTTCCACAGGGAAAGGGTTCAGATTCTGGCCAACTCTGGTGCTGATCTAATTGCATTTGAGACAACTCCGAATAAGCTAGAGGCGAAGGCATATGCTGAACTTCTTGAGGAAGAAGCAATAGATATTCCAGCATGGTTTACTTTTGCTTCTAAAGATGTAATCAATGTGGTGAGTGGTGATTCCATCTTTGAGTGTGCCTCAATTGCTAATGCATGCAAGCAAGTTGTTGCTGTTGGAATCAACTGCACACCACCTAGTTATATCCAGGGGTTGATTTCGTCTATTCGAAAGGTAACAAGCAAACCAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGTCAGAGCAAGCAATGGATTGTAAGTGTCCTCCTATATGAATACACAGTGCTTGTTGCCCTACTCTTTCAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

373

Amino Acids

40.85

Weight (kDa)

5.19

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 101 - 355 2.7e-65 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 94
AciI CCGC 3 cut(s) 288, 312, 453
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 1 cut(s) 561
AfiI CCNNNNNNNGG 2 cut(s) 525, 1028
AgsI TTSAA 3 cut(s) 103, 197, 697
AjnI CCWGG 1 cut(s) 966
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 4 cut(s) 328, 344, 657, 778
AluI AGCT 4 cut(s) 328, 344, 657, 778
Alw26I GTCTC 3 cut(s) 616, 755, 1028
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 3 cut(s) 212, 647, 727
AoxI GGCC 3 cut(s) 465, 486, 729
ApeKI GCWGC 3 cut(s) 152, 217, 641
ApoI RAATTY 1 cut(s) 561
ArsI GACNNNNNNTTYG 4 cut(s) 299, 331, 366, 398
Asp700I GAANNNNTTC 2 cut(s) 701, 717
AspLEI GCGC 2 cut(s) 95, 371
AsuHPI GGTGA 6 cut(s) 76, 182, 386, 883, 890, 1043
AsuII TTCGAA 1 cut(s) 988
BalI TGGCCA 1 cut(s) 731
BbvI GCAGC 3 cut(s) 164, 229, 653
BccI CCATC 4 cut(s) 28, 625, 893, 1037
BceAI ACGGC 2 cut(s) 284, 325
BciT130I CCWGG 1 cut(s) 968
BciVI GTATCC 1 cut(s) 598
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 3 cut(s) 616, 755, 1028
BfaI CTAG 5 cut(s) 126, 635, 686, 779, 957
BfoI RGCGCY 1 cut(s) 372
BfuI GTATCC 1 cut(s) 598
BglI GCCNNNNNGGC 1 cut(s) 671
BisI GCNGC 3 cut(s) 153, 218, 642
BlsI GCNGC 3 cut(s) 154, 219, 643
Bme1390I CCNGG 1 cut(s) 968
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 1 cut(s) 968
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 3 cut(s) 418, 464, 653
BmuI ACTGGG 1 cut(s) 54
BplI GAGNNNNNCTC 4 cut(s) 333, 365, 884, 916
BpmI CTGGAG 3 cut(s) 299, 385, 453
Bpu10I CCTNAGC 2 cut(s) 213, 524
Bpu14I TTCGAA 1 cut(s) 988
BpuEI CTTGAG 1 cut(s) 827
BsaBI GATNNNNATC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 1028
BsaJI CCNNGG 2 cut(s) 501, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 1028
Bse1I ACTGG 2 cut(s) 49, 629
Bse3DI GCAATG 1 cut(s) 1064
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 1 cut(s) 968
BseDI CCNNGG 2 cut(s) 501, 967
BseGI GGATG 2 cut(s) 112, 625
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 525, 1028
BseMI GCAATG 1 cut(s) 1064
BseMII CTCAG 2 cut(s) 204, 538
BseNI ACTGG 2 cut(s) 49, 629
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 3 cut(s) 164, 229, 653
BsgI GTGCAG 1 cut(s) 931
BshFI GGCC 3 cut(s) 467, 488, 731
BslI CCNNNNNNNGG 2 cut(s) 525, 1028
BsmAI GTCTC 3 cut(s) 616, 755, 1028
BsnI GGCC 3 cut(s) 467, 488, 731
Bso31I GGTCTC 1 cut(s) 1028
Bsp119I TTCGAA 1 cut(s) 988
Bsp143I GATC 4 cut(s) 48, 139, 346, 745
BspACI CCGC 3 cut(s) 288, 312, 453
BspANI GGCC 3 cut(s) 467, 488, 731
BspCNI CTCAG 2 cut(s) 205, 537
BspLI GGNNCC 1 cut(s) 664
BspPI GGATC 1 cut(s) 56
BspT104I TTCGAA 1 cut(s) 988
BspTNI GGTCTC 1 cut(s) 1028
BsrDI GCAATG 1 cut(s) 1064
BsrI ACTGG 2 cut(s) 49, 629
BssECI CCNNGG 2 cut(s) 501, 967
BssMI GATC 4 cut(s) 48, 139, 346, 745
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 3 cut(s) 208, 1028, 1093
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 4 cut(s) 91, 916, 920, 1055
BstDEI CTNAG 2 cut(s) 213, 524
BstF5I GGATG 2 cut(s) 112, 625
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 95, 371
BstKTI GATC 4 cut(s) 51, 142, 349, 748
BstMAI GTCTC 3 cut(s) 616, 755, 1028
BstMBI GATC 4 cut(s) 48, 139, 346, 745
BstMWI GCNNNNNNNGC 5 cut(s) 161, 375, 551, 671, 905
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 1 cut(s) 966
BstV1I GCAGC 3 cut(s) 164, 229, 653
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 3 cut(s) 467, 488, 731
BtsCI GGATG 2 cut(s) 112, 625
BtsIMutI CAGTG 2 cut(s) 1033, 1098
Cac8I GCNNGC 4 cut(s) 91, 916, 920, 1055
CaiI CAGNNNCTG 3 cut(s) 212, 647, 727
CfoI GCGC 2 cut(s) 95, 371
CviAII CATG 4 cut(s) 265, 338, 834, 915
DdeI CTNAG 2 cut(s) 213, 524
DpnI GATC 4 cut(s) 50, 141, 348, 747
DpnII GATC 4 cut(s) 48, 139, 346, 745
EaeI YGGCCR 1 cut(s) 729
Eco130I CCWWGG 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 966
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 4 cut(s) 268, 341, 837, 918
FatI CATG 4 cut(s) 264, 337, 833, 914
FauI CCCGC 1 cut(s) 305
FauNDI CATATG 1 cut(s) 793
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 153, 218, 642
FokI GGATG 2 cut(s) 119, 612
Fsp4HI GCNGC 3 cut(s) 153, 218, 642
FspBI CTAG 5 cut(s) 126, 635, 686, 779, 957
FspI TGCGCA 1 cut(s) 94
GlaI GCGC 2 cut(s) 94, 370
GluI GCNGC 3 cut(s) 153, 218, 642
GsuI CTGGAG 3 cut(s) 299, 385, 453
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 3 cut(s) 467, 488, 731
HhaI GCGC 2 cut(s) 95, 371
Hin1II CATG 4 cut(s) 268, 341, 837, 918
Hin6I GCGC 2 cut(s) 93, 369
HinP1I GCGC 2 cut(s) 93, 369
HinfI GANTC 3 cut(s) 724, 881, 939
HphI GGTGA 6 cut(s) 76, 182, 386, 883, 890, 1043
Hpy166II GTNNAC 1 cut(s) 840
Hpy188I TCNGA 4 cut(s) 404, 723, 771, 1050
Hpy8I GTNNAC 1 cut(s) 840
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 5 cut(s) 97, 203, 368, 399, 781
HpyCH4III ACNGT 3 cut(s) 208, 1028, 1093
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 9 cut(s) 152, 412, 545, 579, 641, 755, 914, 918, 948
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 375, 551, 671, 905
HpyF3I CTNAG 2 cut(s) 213, 524
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 4 cut(s) 268, 341, 837, 918
HspAI GCGC 2 cut(s) 93, 369
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 4 cut(s) 48, 139, 346, 745
LmnI GCTCC 5 cut(s) 325, 341, 366, 434, 662
Lsp1109I GCAGC 3 cut(s) 164, 229, 653
LweI GCATC 3 cut(s) 418, 464, 653
MaeI CTAG 5 cut(s) 126, 635, 686, 779, 957
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 64, 679, 994
MalI GATC 4 cut(s) 50, 141, 348, 747
MboI GATC 4 cut(s) 48, 139, 346, 745
MboII GAAGA 2 cut(s) 518, 824
MfeI CAATTG 2 cut(s) 172, 903
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 6 cut(s) 58, 172, 540, 561, 750, 903
MluNI TGGCCA 1 cut(s) 731
MmeI TCCRAC 1 cut(s) 916
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 916
MroXI GAANNNNTTC 2 cut(s) 701, 717
MscI TGGCCA 1 cut(s) 731
Msp20I TGGCCA 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 968
MunI CAATTG 2 cut(s) 172, 903
MvaI CCWGG 1 cut(s) 968
MwoI GCNNNNNNNGC 5 cut(s) 161, 375, 551, 671, 905
NdeI CATATG 1 cut(s) 793
NdeII GATC 4 cut(s) 48, 139, 346, 745
NlaIII CATG 4 cut(s) 268, 341, 837, 918
NlaIV GGNNCC 1 cut(s) 664
NmuCI GTSAC 1 cut(s) 64
NsbI TGCGCA 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 1 cut(s) 918
NspV TTCGAA 1 cut(s) 988
PaeI GCATGC 1 cut(s) 918
PdmI GAANNNNTTC 2 cut(s) 701, 717
PfeI GAWTC 3 cut(s) 724, 881, 939
PkrI GCNGC 3 cut(s) 154, 219, 643
Psp6I CCWGG 1 cut(s) 966
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 1 cut(s) 664
PstNI CAGNNNCTG 3 cut(s) 212, 647, 727
SatI GCNGC 3 cut(s) 153, 218, 642
Sau3AI GATC 4 cut(s) 48, 139, 346, 745
ScrFI CCNGG 1 cut(s) 968
SfaNI GCATC 3 cut(s) 418, 464, 653
SfuI TTCGAA 1 cut(s) 988
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
SphI GCATGC 1 cut(s) 918
Sse9I AATT 6 cut(s) 58, 172, 540, 561, 750, 903
SsiI CCGC 3 cut(s) 288, 312, 453
SspMI CTAG 5 cut(s) 126, 635, 686, 779, 957
StyD4I CCNGG 1 cut(s) 966
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 3 cut(s) 208, 1028, 1093
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 30, 549, 620, 988
TasI AATT 6 cut(s) 58, 172, 540, 561, 750, 903
TfiI GAWTC 3 cut(s) 724, 881, 939
TscAI CASTG 2 cut(s) 1033, 1098
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 3 cut(s) 152, 217, 641
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 2 cut(s) 172, 1098
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 2 cut(s) 1033, 1098
XapI RAATTY 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 1 cut(s) 918
XmnI GAANNNNTTC 2 cut(s) 701, 717
XspI CTAG 5 cut(s) 126, 635, 686, 779, 957
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.