Rw4G000920

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002729
Physical Location & Seq
Reverse (-)
1844796 .. 1846064
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000920.1

Sequence Viewer

Length: 408 bp
ATGCTGAATGCTGATCTAACTGAATTTGGGATGATCACAAATGAGCTAGAAGCAAAGTGGGAAATATATACTTTCACTTGGAATGGTTGCAGATTCAAGCAAAATCGGGTACATGCCGATCTGTCTGAAGTTGAGACGACTGCAAATGAACTAGAAGGAAAGGTAACAAGCAAACTAATAGTGATATACCCCAACAGTGGTGAGACCTATGATGGCCAGAGCAGGCAAATGGGTGGTAAATCTCATTGTTCATCAAGTAGGTGGGTTGATCAAGTGTCTGCAGACATTGTCATAGAGAAGTGGCGTGAGGCTGGGGCTTCCCTGTTTGGAGGCTGCAGCAGAACAACTCCAAATACCATCACGGCAATATCTAAGGCTCTTTATAACAAGCTAGTCTTTTGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

135

Amino Acids

15.08

Weight (kDa)

5.69

Isoelectric Point (pI)

27.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 42 - 127 5.8e-11 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 2 cut(s) 46, 391
AluI AGCT 2 cut(s) 46, 391
Alw26I GTCTC 2 cut(s) 128, 197
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 2 cut(s) 333, 336
ApoI RAATTY 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 212
BalI TGGCCA 1 cut(s) 216
BbvI GCAGC 2 cut(s) 320, 348
BccI CCATC 2 cut(s) 206, 365
BceAI ACGGC 1 cut(s) 378
BclI TGATCA 2 cut(s) 33, 268
BcoDI GTCTC 2 cut(s) 128, 197
BfaI CTAG 3 cut(s) 47, 152, 392
BfmI CTRYAG 2 cut(s) 279, 334
BisI GCNGC 2 cut(s) 334, 337
BlsI GCNGC 2 cut(s) 335, 338
BsaI GGTCTC 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 197
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 320, 348
BseYI CCCAGC 1 cut(s) 311
BshFI GGCC 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 2 cut(s) 128, 197
BsmBI CGTCTC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 1 cut(s) 216
Bso31I GGTCTC 1 cut(s) 197
Bsp143I GATC 4 cut(s) 13, 33, 118, 268
BspANI GGCC 1 cut(s) 216
BspMAI CTGCAG 2 cut(s) 283, 338
BspTNI GGTCTC 1 cut(s) 197
BssMI GATC 4 cut(s) 13, 33, 118, 268
Bst4CI ACNGT 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 4 cut(s) 16, 36, 121, 271
BstMAI GTCTC 2 cut(s) 128, 197
BstMBI GATC 4 cut(s) 13, 33, 118, 268
BstNSI RCATGY 1 cut(s) 116
BstSFI CTRYAG 2 cut(s) 279, 334
BstV1I GCAGC 2 cut(s) 320, 348
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 110
CviAII CATG 1 cut(s) 113
CviJI RGCY 7 cut(s) 46, 216, 311, 317, 333, 377, 391
CviKI_1 RGCY 7 cut(s) 46, 216, 311, 317, 333, 377, 391
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 1 cut(s) 372
DpnI GATC 4 cut(s) 15, 35, 120, 270
DpnII GATC 4 cut(s) 13, 33, 118, 268
EaeI YGGCCR 1 cut(s) 214
Eco31I GGTCTC 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 1 cut(s) 116
FaiI YATR 7 cut(s) 67, 69, 114, 187, 210, 293, 384
FalI AAGNNNNNCTT 1 cut(s) 380
FatI CATG 1 cut(s) 112
FbaI TGATCA 2 cut(s) 33, 268
Fnu4HI GCNGC 2 cut(s) 334, 337
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 334, 337
FspBI CTAG 3 cut(s) 47, 152, 392
GluI GCNGC 2 cut(s) 334, 337
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 1 cut(s) 216
Hin1II CATG 1 cut(s) 116
HinfI GANTC 1 cut(s) 93
HphI GGTGA 1 cut(s) 212
Hpy188I TCNGA 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 5 cut(s) 90, 143, 281, 336, 402
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 1 cut(s) 116
Ksp22I TGATCA 2 cut(s) 33, 268
Kzo9I GATC 4 cut(s) 13, 33, 118, 268
LpnPI CCDG 4 cut(s) 208, 230, 297, 335
Lsp1109I GCAGC 2 cut(s) 320, 348
MaeI CTAG 3 cut(s) 47, 152, 392
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 15, 35, 120, 270
MboI GATC 4 cut(s) 13, 33, 118, 268
MlsI TGGCCA 1 cut(s) 216
MluCI AATT 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 216
MnlI CCTC 2 cut(s) 301, 323
Mox20I TGGCCA 1 cut(s) 216
MscI TGGCCA 1 cut(s) 216
MseI TTAA 1 cut(s) 406
Msp20I TGGCCA 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 13
NdeII GATC 4 cut(s) 13, 33, 118, 268
NlaIII CATG 1 cut(s) 116
NspI RCATGY 1 cut(s) 116
PctI GAATGC 1 cut(s) 13
PfeI GAWTC 1 cut(s) 93
PflFI GACNNNGTC 1 cut(s) 287
PkrI GCNGC 2 cut(s) 335, 338
PsiI TTATAA 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 311
PstI CTGCAG 2 cut(s) 283, 338
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 406
SatI GCNGC 2 cut(s) 334, 337
Sau3AI GATC 4 cut(s) 13, 33, 118, 268
SetI ASST 5 cut(s) 48, 165, 209, 263, 393
SfcI CTRYAG 2 cut(s) 279, 334
Sse9I AATT 1 cut(s) 23
SspMI CTAG 3 cut(s) 47, 152, 392
TaaI ACNGT 1 cut(s) 197
TasI AATT 1 cut(s) 23
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 406
Tru9I TTAA 1 cut(s) 406
TscAI CASTG 1 cut(s) 202
TseI GCWGC 2 cut(s) 333, 336
TspDTI ATGAA 2 cut(s) 162, 240
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 287
XapI RAATTY 1 cut(s) 23
XceI RCATGY 1 cut(s) 116
XspI CTAG 3 cut(s) 47, 152, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.