AT3G44695

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002062
Physical Location & Seq
Reverse (-)
16236298 .. 16237095
798 bp
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UTR
Exon/CDS
Intron
AT3G44695.1

Sequence Viewer

Length: 639 bp
ATGCCTCTTATTCCTAAAAAAGAAAAACAAGAACTGGGTCTTCCTCGCTACCTTCGTCGCGGCCGCCCTTTTCTAAAACTGATCCCCTCAGCAACAAATCTTCGGTTTAGGGGGATAAAGTTCGAACTGAGGAACAATGCAAAAACGCTATTGGATATAAGACACAAAAGAAATCTTCTTGAGATTCCACCAATGATCTTTGATGAATTTCTCATCTTGTTCTTCTTTAATTGTGTTGCCTTTGAAGATTTTTATGCTTATTGTACCAAGCACATAACAAGCTACGTTTTTTTCATGGGATGTCTTCTCGAAAACGAGGACGATGCGAGATTGCTTTGCCGCAAAGGAATTATAACAAACTGTATCGGATCAGTGAATGAGATCTCTCAATTCTATAAGGTCATCGGTACAGATAGCCACTTTTATCTCAACGCGAGTTACTTAGCTGATGTTTTCGAAGGAGTTAATGAATATAGTTCACGAGGATGGCATGCATATTGGTCGGTATTCAAGAATACCCAGCTTACTTACGATACTCCTCGCACATGTCTTTCATGTTGCACTGGTTTGACGATTCTCCTTCTCACGACATTACAAGCCGTCTTTGCAGTGTATGCCTATTATCGTCCTCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

212

Amino Acids

24.76

Weight (kDa)

8.97

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 25 - 195 1.4e-36 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AccII CGCG 2 cut(s) 60, 434
AciI CCGC 3 cut(s) 60, 64, 340
AclWI GGATC 2 cut(s) 76, 376
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 2 cut(s) 265, 409
AflIII ACRYGT 1 cut(s) 545
AgsI TTSAA 2 cut(s) 245, 511
AluBI AGCT 3 cut(s) 282, 446, 523
AluI AGCT 3 cut(s) 282, 446, 523
AlwI GGATC 2 cut(s) 76, 376
AoxI GGCC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 206
AsuII TTCGAA 2 cut(s) 123, 456
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 2 cut(s) 32, 296
BbvCI CCTCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 480
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 483, 517
BglII AGATCT 1 cut(s) 381
BisI GCNGC 3 cut(s) 61, 64, 340
BlsI GCNGC 3 cut(s) 62, 65, 341
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 1 cut(s) 313
BmuI ACTGGG 1 cut(s) 44
BpiI GAAGAC 2 cut(s) 32, 296
Bpu10I CCTNAGC 1 cut(s) 88
Bpu14I TTCGAA 2 cut(s) 123, 456
BpuEI CTTGAG 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 39, 568
BseGI GGATG 2 cut(s) 305, 491
BseMII CTCAG 2 cut(s) 102, 119
BseNI ACTGG 2 cut(s) 39, 568
BseRI GAGGAG 1 cut(s) 528
BseX3I CGGCCG 1 cut(s) 61
BseYI CCCAGC 1 cut(s) 519
Bsh1236I CGCG 2 cut(s) 60, 434
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 64
BsnI GGCC 1 cut(s) 63
Bsp119I TTCGAA 2 cut(s) 123, 456
Bsp143I GATC 4 cut(s) 81, 195, 368, 381
BspACI CCGC 3 cut(s) 60, 64, 340
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 2 cut(s) 101, 120
BspFNI CGCG 2 cut(s) 60, 434
BspPI GGATC 2 cut(s) 76, 376
BspT104I TTCGAA 2 cut(s) 123, 456
BsrI ACTGG 2 cut(s) 39, 568
BssMI GATC 4 cut(s) 81, 195, 368, 381
BssSI CACGAG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 614
BstBI TTCGAA 2 cut(s) 123, 456
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 3 cut(s) 88, 128, 442
BstF5I GGATG 2 cut(s) 305, 491
BstFNI CGCG 2 cut(s) 60, 434
BstKTI GATC 4 cut(s) 84, 198, 371, 384
BstMBI GATC 4 cut(s) 81, 195, 368, 381
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 605, 614
BstNSI RCATGY 2 cut(s) 494, 549
BstUI CGCG 2 cut(s) 60, 434
BstV2I GAAGAC 2 cut(s) 32, 296
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 1 cut(s) 63
BtsCI GGATG 2 cut(s) 305, 491
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 378, 561, 615
Cac8I GCNNGC 1 cut(s) 492
CciNI GCGGCCGC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 264, 408
CviAII CATG 4 cut(s) 295, 491, 546, 555
CviJI RGCY 6 cut(s) 63, 282, 417, 446, 523, 599
CviKI_1 RGCY 6 cut(s) 63, 282, 417, 446, 523, 599
CviQI GTAC 2 cut(s) 264, 408
DdeI CTNAG 3 cut(s) 88, 128, 442
DpnI GATC 4 cut(s) 83, 197, 370, 383
DpnII GATC 4 cut(s) 81, 195, 368, 381
EaeI YGGCCR 1 cut(s) 61
EagI CGGCCG 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 61
Eco52I CGGCCG 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 496
FaeI CATG 4 cut(s) 298, 494, 549, 558
FatI CATG 4 cut(s) 294, 490, 545, 554
Fnu4HI GCNGC 3 cut(s) 61, 64, 340
FokI GGATG 2 cut(s) 312, 498
Fsp4HI GCNGC 3 cut(s) 61, 64, 340
GluI GCNGC 3 cut(s) 61, 64, 340
GsaI CCCAGC 1 cut(s) 523
HaeIII GGCC 1 cut(s) 63
Hin1II CATG 4 cut(s) 298, 494, 549, 558
HinfI GANTC 2 cut(s) 184, 574
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 2 cut(s) 368, 633
Hpy188III TCNNGA 5 cut(s) 179, 308, 480, 511, 586
Hpy8I GTNNAC 1 cut(s) 479
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 3 cut(s) 62, 452, 590
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 4 cut(s) 140, 494, 561, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 614
HpyF3I CTNAG 3 cut(s) 88, 128, 442
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 4 cut(s) 298, 494, 549, 558
Kzo9I GATC 4 cut(s) 81, 195, 368, 381
LpnPI CCDG 3 cut(s) 20, 533, 549
LweI GCATC 1 cut(s) 313
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 437
MalI GATC 4 cut(s) 83, 197, 370, 383
MboI GATC 4 cut(s) 81, 195, 368, 381
MboII GAAGA 6 cut(s) 32, 92, 167, 214, 257, 296
MflI RGATCY 1 cut(s) 381
MluCI AATT 4 cut(s) 206, 229, 348, 389
MnlI CCTC 8 cut(s) 15, 54, 97, 123, 310, 476, 549, 639
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 2 cut(s) 228, 465
MslI CAYNNNNRTG 1 cut(s) 484
MvnI CGCG 2 cut(s) 60, 434
MwoI GCNNNNNNNGC 2 cut(s) 605, 614
NdeII GATC 4 cut(s) 81, 195, 368, 381
NlaIII CATG 4 cut(s) 298, 494, 549, 558
NotI GCGGCCGC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 2 cut(s) 494, 549
NspV TTCGAA 2 cut(s) 123, 456
PaeI GCATGC 1 cut(s) 494
PciI ACATGT 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PfeI GAWTC 2 cut(s) 184, 574
PkrI GCNGC 3 cut(s) 62, 65, 341
PscI ACATGT 1 cut(s) 545
PsiI TTATAA 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 519
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 2 cut(s) 265, 409
RsaNI GTAC 2 cut(s) 264, 408
RseI CAYNNNNRTG 1 cut(s) 484
SaqAI TTAA 2 cut(s) 228, 465
SatI GCNGC 3 cut(s) 61, 64, 340
Sau3AI GATC 4 cut(s) 81, 195, 368, 381
SetI ASST 6 cut(s) 54, 284, 288, 402, 448, 525
SfaNI GCATC 1 cut(s) 313
SfuI TTCGAA 2 cut(s) 123, 456
SmiMI CAYNNNNRTG 1 cut(s) 484
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SphI GCATGC 1 cut(s) 494
Sse9I AATT 4 cut(s) 206, 229, 348, 389
SsiI CCGC 3 cut(s) 60, 64, 340
TaaI ACNGT 1 cut(s) 362
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 123, 309, 456
TasI AATT 4 cut(s) 206, 229, 348, 389
TauI GCSGC 3 cut(s) 63, 66, 342
TfiI GAWTC 2 cut(s) 184, 574
Tru1I TTAA 2 cut(s) 228, 465
Tru9I TTAA 2 cut(s) 228, 465
TscAI CASTG 3 cut(s) 378, 568, 615
TspDTI ATGAA 4 cut(s) 219, 283, 483, 543
TspRI CASTG 3 cut(s) 378, 568, 615
XapI RAATTY 1 cut(s) 206
XceI RCATGY 2 cut(s) 494, 549
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.