AT5G22550

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002062
Physical Location & Seq
Reverse (-)
7483863 .. 7485482
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22550.1

Sequence Viewer

Length: 1332 bp
ATGGATCCTCCCAGCCAAGCAAACGGAGAGAGTGCCACGATCCCAGACGAAAACGAAGAAGAAGCTTATTTGCTCTTAGATAAAGTGATCCCACCAGTCCATCTCAAGGAATCAGCAGGTGATAGATTTTGTTGCATCTACAGAATCCCTCACACTCTCAAGCAAGTCAACGACAAAGCTTATGCACCTAAGATCGTTTCAATAGGTCCTTATCACCATTCGAGTGATAAACAACATCTCAAGATGATAGAGGAGCACAAGAAACGTTATTTGGAGATGTTCGTGTCAAAGACTAAGGAAAATGTGGTTTCACAAAAGATTGAATACACAAATCTCAAAGACCCAATTTTCAAGCTACGATGGATATTGCCAACACTTCGAAGCGACCTTCTTCTTCTCGAAAACCAGGTTCCTCTCTTTCTACTTAAAGTTCTTTTAGAGACATCAAAGTTAGCTCCATCCACTAGCTTGAACATGTTGGCCTTCAAATTCTTCGACTACTCCATAAAAAAACCAGAAGGGTTCTGGGAGAAACACAACAATCTTCGAGCAAAACATCTTCTTGATCTTATTCGTAAGACTTTCATACCCGCCCCGCCTCCATCAACCACCCCCAGGCAATGTTGTATCAATATTTTCAATGGTCCTAGGGAATATTCAAGAACAGAGACATCCAAAAATATTTGTCTCGGTAAGATAAGTTGTTCAAAGGAGATAACGGGAGCGCAAACATCATCGCCTCCTCCTCCTCCTCGTCGTCCTTTTCTTGGGCTAATTGTCTCAGCAAGAAAACTACGACTTCGGGGAATAAAATTTATGCGGAAGGAGAATGTTGAGACACCGTTAGACATAAGTTTCAAGAGTGGTTTGGTTGAAATACCACTACTAGTGTTTGACGACTTTATCAGCAATCTTTTGATCAACTGTGTTGCCTTTGAGCAGTTTAATATGAGCTGCTCCACTGAGATTACTAGCTTTGTAATTTTCATGGGTTGCTTGATCAACACAGAAGACGATGCGACTTTCTTAATCGAGAAAGGGATCTTAGAGAACTATTTTGGAACGGGTGAAGAAGTTTCTTTATTTTTTAAGAACATTGGAAAAGACATCTCATTCAGCATATCCAAGAGTTTCTTGTCAAATGTGTTCGAGGGAGTGAATGAGTACACTTCACAAGGATACCACGTACACTGGGCAGGGTTCAAGTACACACATTTCAACACTCCATGGACATTTCTATCATCTTGTGCTGCTTTGGTGCTTCTTCTCCTTACCATTTTCCAAGCCTTCTTTGCAGCTTATGCTTACTTTCGTCCTCCCAAAAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

443

Amino Acids

50.6

Weight (kDa)

8.64

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 46 - 101 1.5e-16 Plant protein of unknown function
DUF247 PF03140 111 - 424 3.5e-69 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 107
AciI CCGC 3 cut(s) 591, 596, 820
AclI AACGTT 1 cut(s) 265
AclWI GGATC 4 cut(s) 12, 34, 82, 1049
AcsI RAATTY 2 cut(s) 488, 812
AfaI GTAC 3 cut(s) 1166, 1188, 1208
AfiI CCNNNNNNNGG 3 cut(s) 106, 615, 767
AflIII ACRYGT 1 cut(s) 474
AhlI ACTAGT 1 cut(s) 886
AjnI CCWGG 2 cut(s) 405, 614
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 8 cut(s) 65, 179, 355, 455, 468, 954, 975, 1298
AluI AGCT 8 cut(s) 65, 179, 355, 455, 468, 954, 975, 1298
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 5 cut(s) 434, 662, 692, 784, 830
AlwI GGATC 4 cut(s) 12, 34, 82, 1049
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 954, 1250, 1295
ApoI RAATTY 2 cut(s) 488, 812
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 2 cut(s) 206, 644
AsuHPI GGTGA 3 cut(s) 131, 206, 1079
AsuII TTCGAA 1 cut(s) 379
AvaII GGWCC 2 cut(s) 206, 644
AvrII CCTAGG 1 cut(s) 647
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 941, 1237, 1307
BccI CCATC 4 cut(s) 108, 354, 466, 610
BciT130I CCWGG 2 cut(s) 407, 616
BciVI GTATCC 1 cut(s) 1172
BclI TGATCA 2 cut(s) 918, 999
BcoDI GTCTC 5 cut(s) 434, 662, 692, 784, 830
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 4 cut(s) 465, 648, 887, 972
BfmI CTRYAG 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 107
BfuI GTATCC 1 cut(s) 1172
BisI GCNGC 3 cut(s) 955, 1251, 1296
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 3 cut(s) 956, 1252, 1297
Bme1390I CCNGG 2 cut(s) 407, 616
Bme18I GGWCC 2 cut(s) 206, 644
BmgT120I GGNCC 2 cut(s) 206, 644
BmiI GGNNCC 2 cut(s) 6, 411
BmrFI CCNGG 2 cut(s) 407, 616
BmrI ACTGGG 1 cut(s) 1201
BmsI GCATC 2 cut(s) 144, 1006
BmuI ACTGGG 1 cut(s) 1201
BpiI GAAGAC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 379
BpuEI CTTGAG 3 cut(s) 89, 143, 224
BsaAI YACGTR 1 cut(s) 1186
BsaJI CCNNGG 3 cut(s) 614, 647, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 615, 767
Bse1I ACTGG 2 cut(s) 95, 1196
Bse3DI GCAATG 1 cut(s) 626
BseBI CCWGG 2 cut(s) 407, 616
BseDI CCNNGG 3 cut(s) 614, 647, 1226
BseGI GGATG 2 cut(s) 458, 671
BseLI CCNNNNNNNGG 3 cut(s) 106, 615, 767
BseMI GCAATG 1 cut(s) 626
BseMII CTCAG 2 cut(s) 795, 954
BseNI ACTGG 2 cut(s) 95, 1196
BseRI GAGGAG 5 cut(s) 266, 732, 735, 738, 741
BseXI GCAGC 3 cut(s) 941, 1237, 1307
BseYI CCCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 258
BslI CCNNNNNNNGG 3 cut(s) 106, 615, 767
BsmAI GTCTC 5 cut(s) 434, 662, 692, 784, 830
BsnI GGCC 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
Bsp19I CCATGG 1 cut(s) 1226
BspACI CCGC 3 cut(s) 591, 596, 820
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 2 cut(s) 794, 955
BspLI GGNNCC 2 cut(s) 6, 411
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 4 cut(s) 12, 34, 82, 1049
BspT104I TTCGAA 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 626
BsrI ACTGG 2 cut(s) 95, 1196
BssECI CCNNGG 3 cut(s) 614, 647, 1226
BssMI GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
BssT1I CCWWGG 2 cut(s) 647, 1226
Bst2UI CCWGG 2 cut(s) 407, 616
Bst4CI ACNGT 2 cut(s) 843, 926
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstBAI YACGTR 1 cut(s) 1186
BstBI TTCGAA 1 cut(s) 379
BstDEI CTNAG 6 cut(s) 76, 189, 294, 781, 963, 1045
BstDSI CCRYGG 1 cut(s) 1226
BstF5I GGATG 2 cut(s) 458, 671
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 8 cut(s) 7, 42, 90, 195, 568, 921, 1002, 1044
BstMAI GTCTC 5 cut(s) 434, 662, 692, 784, 830
BstMBI GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
BstMWI GCNNNNNNNGC 2 cut(s) 1292, 1301
BstNI CCWGG 2 cut(s) 407, 616
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 405, 614
BstSFI CTRYAG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 941, 1237, 1307
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 2 cut(s) 4, 1041
BstYI RGATCY 2 cut(s) 4, 1041
BsuI GTATCC 1 cut(s) 1172
BsuRI GGCC 1 cut(s) 482
BtgI CCRYGG 1 cut(s) 1226
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 2 cut(s) 458, 671
BtsIMutI CAGTG 2 cut(s) 960, 1189
BveI ACCTGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 2 cut(s) 206, 644
CsiI ACCWGGT 1 cut(s) 405
Csp6I GTAC 3 cut(s) 1165, 1187, 1207
CviAII CATG 3 cut(s) 475, 988, 1227
CviQI GTAC 3 cut(s) 1165, 1187, 1207
DdeI CTNAG 6 cut(s) 76, 189, 294, 781, 963, 1045
DpnI GATC 8 cut(s) 6, 41, 89, 194, 567, 920, 1001, 1043
DpnII GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
Eco130I CCWWGG 2 cut(s) 647, 1226
Eco47I GGWCC 2 cut(s) 206, 644
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 405, 614
EcoT14I CCWWGG 2 cut(s) 647, 1226
ErhI CCWWGG 2 cut(s) 647, 1226
FaeI CATG 3 cut(s) 478, 991, 1230
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FatI CATG 3 cut(s) 474, 987, 1226
FauI CCCGC 2 cut(s) 598, 603
FbaI TGATCA 2 cut(s) 918, 999
Fnu4HI GCNGC 3 cut(s) 955, 1251, 1296
FokI GGATG 2 cut(s) 445, 658
Fsp4HI GCNGC 3 cut(s) 955, 1251, 1296
FspBI CTAG 4 cut(s) 465, 648, 887, 972
GlaI GCGC 1 cut(s) 726
GluI GCNGC 3 cut(s) 955, 1251, 1296
GsaI CCCAGC 1 cut(s) 15
HaeIII GGCC 1 cut(s) 482
HhaI GCGC 1 cut(s) 727
Hin1II CATG 3 cut(s) 478, 991, 1230
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 63, 177
HinfI GANTC 2 cut(s) 110, 144
HphI GGTGA 3 cut(s) 131, 206, 1079
Hpy166II GTNNAC 4 cut(s) 169, 1167, 1189, 1209
Hpy188III TCNNGA 6 cut(s) 241, 398, 563, 660, 859, 1033
Hpy8I GTNNAC 4 cut(s) 169, 1167, 1189, 1209
Hpy99I CGWCG 1 cut(s) 759
HpyAV CCTTC 5 cut(s) 398, 493, 512, 817, 1297
HpyCH4III ACNGT 2 cut(s) 843, 926
HpyCH4IV ACGT 2 cut(s) 265, 1185
HpyCH4V TGCA 3 cut(s) 135, 185, 1295
HpyF10VI GCNNNNNNNGC 2 cut(s) 1292, 1301
HpyF3I CTNAG 6 cut(s) 76, 189, 294, 781, 963, 1045
HpySE526I ACGT 2 cut(s) 265, 1185
Hsp92II CATG 3 cut(s) 478, 991, 1230
HspAI GCGC 1 cut(s) 725
Ksp22I TGATCA 2 cut(s) 918, 999
Kzo9I GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
LmnI GCTCC 4 cut(s) 253, 460, 722, 962
Lsp1109I GCAGC 3 cut(s) 941, 1237, 1307
LweI GCATC 2 cut(s) 144, 1006
MabI ACCWGGT 1 cut(s) 405
MaeI CTAG 4 cut(s) 465, 648, 887, 972
MaeII ACGT 2 cut(s) 265, 1185
MalI GATC 8 cut(s) 6, 41, 89, 194, 567, 920, 1001, 1043
MboI GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
MfeI CAATTG 1 cut(s) 1327
MflI RGATCY 2 cut(s) 4, 1041
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 6 cut(s) 345, 488, 774, 812, 981, 1327
MseI TTAA 4 cut(s) 426, 945, 1028, 1089
MslI CAYNNNNRTG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 407, 616
MunI CAATTG 1 cut(s) 1327
MvaI CCWGG 2 cut(s) 407, 616
MwoI GCNNNNNNNGC 2 cut(s) 1292, 1301
NcoI CCATGG 1 cut(s) 1226
NdeII GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
NlaIII CATG 3 cut(s) 478, 991, 1230
NlaIV GGNNCC 2 cut(s) 6, 411
NspI RCATGY 1 cut(s) 478
NspV TTCGAA 1 cut(s) 379
PaqCI CACCTGC 1 cut(s) 107
PciI ACATGT 1 cut(s) 474
PfeI GAWTC 2 cut(s) 110, 144
PkrI GCNGC 3 cut(s) 956, 1252, 1297
Ppu21I YACGTR 1 cut(s) 1186
PpuMI RGGWCCY 1 cut(s) 206
PscI ACATGT 1 cut(s) 474
Psp1406I AACGTT 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 405, 614
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 405, 614
PspN4I GGNNCC 2 cut(s) 6, 411
PspPI GGNCC 2 cut(s) 206, 644
PspPPI RGGWCCY 1 cut(s) 206
PsuI RGATCY 2 cut(s) 4, 1041
RsaI GTAC 3 cut(s) 1166, 1188, 1208
RsaNI GTAC 3 cut(s) 1165, 1187, 1207
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 4 cut(s) 426, 945, 1028, 1089
SatI GCNGC 3 cut(s) 955, 1251, 1296
Sau3AI GATC 8 cut(s) 4, 39, 87, 192, 565, 918, 999, 1041
Sau96I GGNCC 2 cut(s) 206, 644
ScrFI CCNGG 2 cut(s) 407, 616
SduI GDGCHC 1 cut(s) 258
SexAI ACCWGGT 1 cut(s) 405
SfaNI GCATC 2 cut(s) 144, 1006
SfcI CTRYAG 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 2 cut(s) 206, 644
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 3 cut(s) 104, 158, 239
SmoI CTYRAG 3 cut(s) 104, 158, 239
SpeI ACTAGT 1 cut(s) 886
Sse9I AATT 6 cut(s) 345, 488, 774, 812, 981, 1327
SsiI CCGC 3 cut(s) 591, 596, 820
SspI AATATT 3 cut(s) 634, 656, 682
SspMI CTAG 4 cut(s) 465, 648, 887, 972
StyD4I CCNGG 2 cut(s) 405, 614
StyI CCWWGG 2 cut(s) 647, 1226
TaaI ACNGT 2 cut(s) 843, 926
TaiI ACGT 2 cut(s) 268, 1188
TaqI TCGA 7 cut(s) 221, 379, 399, 495, 547, 1032, 1149
TasI AATT 6 cut(s) 345, 488, 774, 812, 981, 1327
TatI WGTACW 2 cut(s) 1164, 1206
TfiI GAWTC 2 cut(s) 110, 144
Tru1I TTAA 4 cut(s) 426, 945, 1028, 1089
Tru9I TTAA 4 cut(s) 426, 945, 1028, 1089
TscAI CASTG 2 cut(s) 967, 1196
TseI GCWGC 3 cut(s) 954, 1250, 1295
TspDTI ATGAA 2 cut(s) 574, 976
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 2 cut(s) 967, 1196
VpaK11BI GGWCC 2 cut(s) 206, 644
XapI RAATTY 2 cut(s) 488, 812
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 522
XmaJI CCTAGG 1 cut(s) 647
XspI CTAG 4 cut(s) 465, 648, 887, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.