MD10G1144400.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002062
Physical Location & Seq
Forward (+)
23108780 .. 23109655
876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1144400.v1.1.491

Sequence Viewer

Length: 876 bp
ATGACCCCACTGTGGATCCGCCACTGGCCTCAACTCAGGCTAGACTTTCTTCGGCTAGAAAACCAAATTCCTTTTTTCGTTCTTCAACAGTTATTTGATATGTCGAAACCTTCAAGAGAGGATACTTATCCGTGTCCGCCCCTTGGCAAGGCTGCTTTAAAATTCTTTAACTACGTAGTGCAACAAAGATCGGACAAGGTCTTGGAGCGATATTTCTATGTAGGCGGAGTACATCTACTCGATTTATTTCACTCAATTTTTACCACCGGTATTCGTGATCCTCCTCGAGAAAATCCTTCTCAGTCAGTTGAATTGATCGGAACAGTGAAAAAGCTTCATCAAGCGGGAATTAAGTTGAAGAAAAGCAAGGCAACCAACTTCCTATGTATCAGATTCTGCAATGGAGTGCTAGAAATTCCGCACGTAATAGTAGACGATTACAGTATCCACGTACTCCTGAATTTCGTTGCATTCGAACAATGTTATATCGATTGCGACAAGCACATAATCACTTATGCTGCATTCATGAACTGCCTCATCTGCAGGCCTGCGGATGCAACCTACCTCTCTGAGAAGAACATCATCGAGAATTATCTTGGATCCGACGAGGAGCTCACTCAATTCTTCAACAATATGAGCAAAGATGTTGGTTTCGGCGTCATTGGGAGTTACTTACAGCAGGTATTCAAGGATGTGAACGAGCACTGCGATAATGTTTGGAACGTGCGATGGGCAGGTTTTATGTTGAAGTATTTTAGAAGCCCTTGGTCTTTTATGTCTGCCTCAGCTGTTTTGAAAGCCTTATTACTCACAGTAATCCAGACCGTTTTATCCATTTTTCAATTTAACGGTCCACAGGAGCGTCGCGGAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

292

Amino Acids

33.68

Weight (kDa)

8.07

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 9 - 269 1.1e-69 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 670, 725
AccI GTMKAC 1 cut(s) 432
AccII CGCG 1 cut(s) 867
AciI CCGC 7 cut(s) 19, 137, 225, 344, 419, 551, 867
AclWI GGATC 5 cut(s) 10, 23, 272, 594, 607
AcsI RAATTY 4 cut(s) 66, 161, 414, 460
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 2 cut(s) 231, 453
AfiI CCNNNNNNNGG 3 cut(s) 12, 143, 148
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 9 cut(s) 86, 114, 311, 358, 628, 688, 748, 796, 842
AluBI AGCT 3 cut(s) 334, 613, 788
AluI AGCT 3 cut(s) 334, 613, 788
Alw21I GWGCWC 2 cut(s) 615, 705
AlwI GGATC 5 cut(s) 10, 23, 272, 594, 607
AlwNI CAGNNNCTG 1 cut(s) 396
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 2 cut(s) 26, 545
ApeKI GCWGC 2 cut(s) 152, 518
ApoI RAATTY 4 cut(s) 66, 161, 414, 460
AsiGI ACCGGT 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 851
AsuII TTCGAA 1 cut(s) 474
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 851
BamHI GGATCC 2 cut(s) 15, 599
BanII GRGCYC 1 cut(s) 615
Bbv12I GWGCWC 2 cut(s) 615, 705
BbvCI CCTCAGC 1 cut(s) 784
BbvI GCAGC 2 cut(s) 139, 505
BccI CCATC 1 cut(s) 723
BciVI GTATCC 2 cut(s) 115, 455
BfaI CTAG 3 cut(s) 41, 56, 410
BfmI CTRYAG 1 cut(s) 541
BfuAI ACCTGC 2 cut(s) 670, 725
BfuI GTATCC 2 cut(s) 115, 455
BisI GCNGC 2 cut(s) 153, 519
BlsI GCNGC 2 cut(s) 154, 520
Bme18I GGWCC 1 cut(s) 851
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 851
BmiI GGNNCC 2 cut(s) 17, 601
BmsI GCATC 1 cut(s) 544
Bpu10I CCTNAGC 1 cut(s) 784
Bpu14I TTCGAA 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 489
BsaAI YACGTR 3 cut(s) 175, 424, 451
BsaBI GATNNNNATC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 142, 764
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 143, 148
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 142, 764
BseGI GGATG 2 cut(s) 559, 697
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 12, 143, 148
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 4 cut(s) 49, 314, 561, 798
BseNI ACTGG 1 cut(s) 29
BseRI GAGGAG 2 cut(s) 273, 623
BseXI GCAGC 2 cut(s) 139, 505
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 2 cut(s) 28, 547
BshTI ACCGGT 1 cut(s) 266
BshVI ATCGAT 1 cut(s) 489
BsiHKAI GWGCWC 2 cut(s) 615, 705
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 267
BslI CCNNNNNNNGG 3 cut(s) 12, 143, 148
BsmI GAATGC 2 cut(s) 470, 521
BsnI GGCC 2 cut(s) 28, 547
BsoBI CYCGRG 1 cut(s) 285
Bsp119I TTCGAA 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 615, 705
Bsp143I GATC 5 cut(s) 15, 188, 277, 315, 599
BspACI CCGC 7 cut(s) 19, 137, 225, 344, 419, 551, 867
BspANI GGCC 2 cut(s) 28, 547
BspCNI CTCAG 4 cut(s) 48, 313, 562, 797
BspDI ATCGAT 1 cut(s) 489
BspFNI CGCG 1 cut(s) 867
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 17, 601
BspMAI CTGCAG 1 cut(s) 545
BspMI ACCTGC 2 cut(s) 670, 725
BspPI GGATC 5 cut(s) 10, 23, 272, 594, 607
BspT104I TTCGAA 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 142, 764
BssMI GATC 5 cut(s) 15, 188, 277, 315, 599
BssNI GRCGYC 1 cut(s) 657
BssT1I CCWWGG 2 cut(s) 142, 764
Bst4CI ACNGT 7 cut(s) 12, 90, 325, 443, 814, 826, 851
BstACI GRCGYC 1 cut(s) 657
BstBAI YACGTR 3 cut(s) 175, 424, 451
BstBI TTCGAA 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 545, 549
BstDEI CTNAG 4 cut(s) 35, 300, 570, 784
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 2 cut(s) 559, 697
BstFNI CGCG 1 cut(s) 867
BstKTI GATC 5 cut(s) 18, 191, 280, 318, 602
BstMBI GATC 5 cut(s) 15, 188, 277, 315, 599
BstMWI GCNNNNNNNGC 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 541
BstSNI TACGTA 1 cut(s) 175
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 2 cut(s) 139, 505
BstX2I RGATCY 2 cut(s) 15, 599
BstYI RGATCY 2 cut(s) 15, 599
Bsu15I ATCGAT 1 cut(s) 489
BsuI GTATCC 2 cut(s) 115, 455
BsuRI GGCC 2 cut(s) 28, 547
BsuTUI ATCGAT 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 742
BtsCI GGATG 2 cut(s) 559, 697
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 4 cut(s) 8, 22, 330, 703
BveI ACCTGC 2 cut(s) 670, 725
Cac8I GCNNGC 2 cut(s) 545, 549
CaiI CAGNNNCTG 1 cut(s) 396
CciI TCATGA 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 851
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 2 cut(s) 646, 851
Csp6I GTAC 2 cut(s) 230, 452
CspAI ACCGGT 1 cut(s) 266
CviAII CATG 1 cut(s) 526
CviQI GTAC 2 cut(s) 230, 452
DdeI CTNAG 4 cut(s) 35, 300, 570, 784
DpnI GATC 5 cut(s) 17, 190, 279, 317, 601
DpnII GATC 5 cut(s) 15, 188, 277, 315, 599
DraI TTTAAA 1 cut(s) 159
EciI GGCGGA 3 cut(s) 8, 126, 240
Ecl136II GAGCTC 1 cut(s) 613
Eco105I TACGTA 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 142, 764
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 615
Eco47I GGWCC 1 cut(s) 851
Eco53kI GAGCTC 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 613
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 142, 764
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 2 cut(s) 142, 764
FaeI CATG 1 cut(s) 529
FatI CATG 1 cut(s) 525
FauI CCCGC 1 cut(s) 337
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 153, 519
FokI GGATG 2 cut(s) 566, 704
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 2 cut(s) 153, 519
FspBI CTAG 3 cut(s) 41, 56, 410
GluI GCNGC 2 cut(s) 153, 519
HaeIII GGCC 2 cut(s) 28, 547
HapII CCGG 1 cut(s) 267
HgaI GACGC 2 cut(s) 646, 851
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 1 cut(s) 393
HpaII CCGG 1 cut(s) 267
Hpy166II GTNNAC 3 cut(s) 433, 697, 854
Hpy188I TCNGA 5 cut(s) 193, 320, 392, 571, 604
Hpy188III TCNNGA 7 cut(s) 114, 275, 287, 457, 526, 586, 820
Hpy8I GTNNAC 3 cut(s) 433, 697, 854
Hpy99I CGWCG 2 cut(s) 608, 867
HpyAV CCTTC 2 cut(s) 120, 306
HpyCH4III ACNGT 7 cut(s) 12, 90, 325, 443, 814, 826, 851
HpyCH4IV ACGT 4 cut(s) 174, 423, 450, 723
HpyCH4V TGCA 6 cut(s) 181, 399, 470, 521, 543, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 540
HpyF3I CTNAG 4 cut(s) 35, 300, 570, 784
HpySE526I ACGT 4 cut(s) 174, 423, 450, 723
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 1 cut(s) 529
Kzo9I GATC 5 cut(s) 15, 188, 277, 315, 599
LmnI GCTCC 3 cut(s) 205, 610, 859
Lsp1109I GCAGC 2 cut(s) 139, 505
LweI GCATC 1 cut(s) 544
MaeI CTAG 3 cut(s) 41, 56, 410
MaeII ACGT 4 cut(s) 174, 423, 450, 723
MaeIII GTNAC 1 cut(s) 668
MalI GATC 5 cut(s) 17, 190, 279, 317, 601
MboI GATC 5 cut(s) 15, 188, 277, 315, 599
MboII GAAGA 5 cut(s) 41, 74, 370, 586, 616
MflI RGATCY 2 cut(s) 15, 599
MhlI GDGCHC 2 cut(s) 615, 705
MmeI TCCRAC 1 cut(s) 627
MnlI CCTC 9 cut(s) 39, 112, 291, 294, 545, 575, 601, 793, 863
MseI TTAA 4 cut(s) 158, 168, 351, 846
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 1 cut(s) 267
Mva1269I GAATGC 2 cut(s) 470, 521
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 1 cut(s) 540
NdeII GATC 5 cut(s) 15, 188, 277, 315, 599
NlaIII CATG 1 cut(s) 529
NlaIV GGNNCC 2 cut(s) 17, 601
NspV TTCGAA 1 cut(s) 474
PaeR7I CTCGAG 1 cut(s) 285
PagI TCATGA 1 cut(s) 525
PceI AGGCCT 1 cut(s) 547
PcsI WCGNNNNNNNCGW 2 cut(s) 471, 705
PctI GAATGC 2 cut(s) 470, 521
PfeI GAWTC 1 cut(s) 393
PflFI GACNNNGTC 1 cut(s) 197
PinAI ACCGGT 1 cut(s) 266
PkrI GCNGC 2 cut(s) 154, 520
Ppu21I YACGTR 3 cut(s) 175, 424, 451
Psp124BI GAGCTC 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 17, 601
PspPI GGNCC 1 cut(s) 851
PstI CTGCAG 1 cut(s) 545
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 15, 599
PsyI GACNNNGTC 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 2 cut(s) 231, 453
RsaNI GTAC 2 cut(s) 230, 452
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 4 cut(s) 158, 168, 351, 846
SatI GCNGC 2 cut(s) 153, 519
Sau3AI GATC 5 cut(s) 15, 188, 277, 315, 599
Sau96I GGNCC 1 cut(s) 851
SduI GDGCHC 2 cut(s) 615, 705
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 285
SfuI TTCGAA 1 cut(s) 474
SinI GGWCC 1 cut(s) 851
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
SnaBI TACGTA 1 cut(s) 175
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 7 cut(s) 19, 137, 225, 344, 419, 551, 867
SspMI CTAG 3 cut(s) 41, 56, 410
SstI GAGCTC 1 cut(s) 615
StuI AGGCCT 1 cut(s) 547
StyI CCWWGG 2 cut(s) 142, 764
TaaI ACNGT 7 cut(s) 12, 90, 325, 443, 814, 826, 851
TaiI ACGT 4 cut(s) 177, 426, 453, 726
TaqI TCGA 6 cut(s) 104, 240, 286, 474, 489, 585
TatI WGTACW 1 cut(s) 229
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 4 cut(s) 158, 168, 351, 846
Tru9I TTAA 4 cut(s) 158, 168, 351, 846
TscAI CASTG 4 cut(s) 15, 29, 330, 710
TseI GCWGC 2 cut(s) 152, 518
TspDTI ATGAA 3 cut(s) 326, 514, 542
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 4 cut(s) 15, 29, 330, 710
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 851
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 4 cut(s) 66, 161, 414, 460
XhoI CTCGAG 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 3 cut(s) 41, 56, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.