AT5G22540

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002062
Physical Location & Seq
Reverse (-)
7481397 .. 7482953
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22540.1

Sequence Viewer

Length: 1323 bp
ATGGATCCACAGATTGTTGTAGACATAAAGAGTTTAATCTCAGAAGATAATGGTTTGAAGCTTCTTAGAGAATCAGCTGGTTCAGAGTTATGTTGCATCGTGAGAATCCCACAGAGCCTCGCTCGAATCAACCTCAAAGCTTATGAACCGAAGATTGTCTCCATTGGTCCTTACCATCACGGCAAAGAGCATCTCAAAATGACTCAGCAGCATAAACGCCGATTCTTGAAGTTTTTCGTGGCTAAAATGGAAGAAAAAGGATTTGTTCCTCAAGAATTGGTCAAAGCTGTATCGAGTTTGGAAGGAGTTATACGAGGTTCTTACTCCGAGGATCTAGGTTTAGATTCTGAAAACTTGGTTCAGATGATGGTTCTTGATGGTTGCTTTATCCTCACGTTGTTCTTTGTTGTTTCCGGGAAAGTTGAATACACTAATCTTGATGATCCGATTTTTCGAATGCCGTGGATTTTGCCGTCGATTCGAGCAGATCTTCTCCTTTTGGAAAACCAGGTTCCTTATGTTCTTCTTCAAACTCTATTTGAAACATCGAAGTTAGTTACTTGTAGTGGTTTAAACGAAATCGCCTTTGAATTCTTTAACTACTCATTACAAAAACCAGAGACCTTTTGGGAAAAACATTATGGTCTTGAAGCAAAACATCTTCTTGATTTGATACGTAAGACTTTTGTTCCTGTTCCGTCTCAAAGAAGAATCAAAGATCATAGCAGTAAATCGTCATTCAATGATCATGAATATCTCGGATTTGTTCTCTCTGCCAAGAAGCTTCATCTTCGGGGAATCAAATTCAAACCGAGGAAAAACACAGACTCAATCTTAGACATTAGTTATAGTAATGGTGTGCTTCACATTCCTCCGGTAGTCATGGATGATTTTACCGCCTCGATCTTTTTAAACTGTGTCGCATTTGAGCAATTATATGCTGATTCATCGAACCACATAACGAGCTACGTAGCTTTCATGGCTTGTCTTATAAACGAAGAGAGTGATGCATCGTTCCTTAGCGAAAGGAGGATCCTAGAGAACTATTTTGGAACAGAGGATGAAGTGTCCCGGTTCTATAAACGCATTGGTAAAGACATTGCCCTCGACTTAGAGAAGAGTTACCTAGCAAAGGTGTTTGAAGGAGTTAACGAGTATACTTCTCAAGGATTCCATGTTCATTGTGCAGAGTTTATTCATACGCATTTCGATAGTCCATGGACATTTGCATCATCATTTGCAGCGTTGTTGCTTCTTCTGTTTGCGGCTTTGCAAGTCTTCTTTGCAGCTTATAGTTATTTCCGTCCTCCAAAAGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

440

Amino Acids

50.43

Weight (kDa)

6.22

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 33 - 420 2.8e-116 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 992
AccI GTMKAC 2 cut(s) 21, 1157
AciI CCGC 2 cut(s) 897, 1265
AclWI GGATC 5 cut(s) 12, 339, 437, 1027, 1040
AcsI RAATTY 2 cut(s) 590, 803
AfiI CCNNNNNNNGG 1 cut(s) 1132
AjnI CCWGG 1 cut(s) 507
AloI GAACNNNNNNTCC 2 cut(s) 1161, 1193
AluBI AGCT 8 cut(s) 61, 77, 140, 287, 784, 966, 974, 1289
AluI AGCT 8 cut(s) 61, 77, 140, 287, 784, 966, 974, 1289
Alw26I GTCTC 3 cut(s) 163, 614, 705
AlwI GGATC 5 cut(s) 12, 339, 437, 1027, 1040
ApeKI GCWGC 3 cut(s) 208, 1241, 1286
ApoI RAATTY 2 cut(s) 590, 803
Asp700I GAANNNNTTC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 415, 1072
AsuII TTCGAA 1 cut(s) 454
AvaII GGWCC 1 cut(s) 167
BamHI GGATCC 2 cut(s) 4, 1032
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BbsI GAAGAC 1 cut(s) 1270
BbvI GCAGC 3 cut(s) 220, 1253, 1298
BccI CCATC 3 cut(s) 183, 361, 371
BceAI ACGGC 3 cut(s) 196, 445, 457
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 2 cut(s) 415, 1072
BcoDI GTCTC 3 cut(s) 163, 614, 705
BfaI CTAG 3 cut(s) 335, 1037, 1127
BglII AGATCT 1 cut(s) 487
BisI GCNGC 4 cut(s) 209, 1242, 1266, 1287
BlsI GCNGC 4 cut(s) 210, 1243, 1267, 1288
Bme1390I CCNGG 3 cut(s) 415, 509, 1072
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 3 cut(s) 6, 513, 1034
BmrFI CCNGG 3 cut(s) 415, 509, 1072
BmsI GCATC 5 cut(s) 105, 199, 997, 1019, 1238
BpiI GAAGAC 1 cut(s) 1270
BplI GAGNNNNNCTC 2 cut(s) 106, 138
Bpu10I CCTNAGC 1 cut(s) 1019
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 2 cut(s) 255, 1149
BpuMI CCSGG 2 cut(s) 415, 1072
BsaAI YACGTR 2 cut(s) 677, 970
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 4 cut(s) 327, 461, 812, 1217
BsaWI WCCGGW 1 cut(s) 874
Bsc4I CCNNNNNNNGG 1 cut(s) 1132
Bse3DI GCAATG 1 cut(s) 1098
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 4 cut(s) 327, 461, 812, 1217
BseGI GGATG 2 cut(s) 892, 1066
BseLI CCNNNNNNNGG 1 cut(s) 1132
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 2 cut(s) 54, 218
BseXI GCAGC 3 cut(s) 220, 1253, 1298
BsgI GTGCAG 1 cut(s) 1206
BsiSI CCGG 3 cut(s) 414, 875, 1072
BslFI GGGAC 1 cut(s) 1054
BslI CCNNNNNNNGG 1 cut(s) 1132
BsmAI GTCTC 3 cut(s) 163, 614, 705
BsmBI CGTCTC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 1054
BsmI GAATGC 1 cut(s) 462
Bso31I GGTCTC 1 cut(s) 614
Bsp119I TTCGAA 1 cut(s) 454
Bsp143I GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
Bsp19I CCATGG 1 cut(s) 1217
BspACI CCGC 2 cut(s) 897, 1265
BspCNI CTCAG 2 cut(s) 53, 217
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 3 cut(s) 6, 513, 1034
BspPI GGATC 5 cut(s) 12, 339, 437, 1027, 1040
BspT104I TTCGAA 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 1098
BssECI CCNNGG 4 cut(s) 327, 461, 812, 1217
BssMI GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
BssNAI GTATAC 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 1217
Bst1107I GTATAC 1 cut(s) 1158
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 1 cut(s) 917
Bst6I CTCTTC 2 cut(s) 993, 1112
BstBAI YACGTR 2 cut(s) 677, 970
BstBI TTCGAA 1 cut(s) 454
BstDEI CTNAG 6 cut(s) 40, 65, 204, 835, 1019, 1111
BstDSI CCRYGG 2 cut(s) 461, 1217
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 2 cut(s) 892, 1066
BstKTI GATC 8 cut(s) 7, 334, 445, 490, 721, 748, 906, 1035
BstMAI GTCTC 3 cut(s) 163, 614, 705
BstMBI GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
BstMWI GCNNNNNNNGC 1 cut(s) 980
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 3 cut(s) 413, 507, 1070
BstSNI TACGTA 2 cut(s) 677, 970
BstV1I GCAGC 3 cut(s) 220, 1253, 1298
BstV2I GAAGAC 1 cut(s) 1270
BstX2I RGATCY 4 cut(s) 4, 331, 487, 1032
BstYI RGATCY 4 cut(s) 4, 331, 487, 1032
BstZ17I GTATAC 1 cut(s) 1158
BtgI CCRYGG 2 cut(s) 461, 1217
BtsCI GGATG 2 cut(s) 892, 1066
CciI TCATGA 1 cut(s) 748
Cfr13I GGNCC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 507
CviAII CATG 5 cut(s) 749, 883, 979, 1175, 1218
DdeI CTNAG 6 cut(s) 40, 65, 204, 835, 1019, 1111
DpnI GATC 8 cut(s) 6, 333, 444, 489, 720, 747, 905, 1034
DpnII GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
DraI TTTAAA 2 cut(s) 573, 912
Eam1104I CTCTTC 2 cut(s) 993, 1112
EarI CTCTTC 2 cut(s) 993, 1112
Eco105I TACGTA 2 cut(s) 677, 970
Eco130I CCWWGG 1 cut(s) 1217
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 1217
EcoT22I ATGCAT 1 cut(s) 1012
ErhI CCWWGG 1 cut(s) 1217
Esp3I CGTCTC 1 cut(s) 705
FaeI CATG 5 cut(s) 752, 886, 982, 1178, 1221
FaqI GGGAC 1 cut(s) 1054
FatI CATG 5 cut(s) 748, 882, 978, 1174, 1217
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 2 cut(s) 21, 1157
Fnu4HI GCNGC 4 cut(s) 209, 1242, 1266, 1287
FokI GGATG 2 cut(s) 899, 1073
Fsp4HI GCNGC 4 cut(s) 209, 1242, 1266, 1287
FspBI CTAG 3 cut(s) 335, 1037, 1127
GluI GCNGC 4 cut(s) 209, 1242, 1266, 1287
HapII CCGG 3 cut(s) 414, 875, 1072
Hin1II CATG 5 cut(s) 752, 886, 982, 1178, 1221
HincII GTYRAC 1 cut(s) 1150
HindII GTYRAC 1 cut(s) 1150
HindIII AAGCTT 3 cut(s) 59, 138, 782
HpaI GTTAAC 1 cut(s) 1150
HpaII CCGG 3 cut(s) 414, 875, 1072
Hpy166II GTNNAC 3 cut(s) 22, 1150, 1158
Hpy188I TCNGA 7 cut(s) 43, 85, 328, 349, 363, 447, 761
Hpy188III TCNNGA 8 cut(s) 100, 226, 272, 374, 437, 647, 665, 749
Hpy8I GTNNAC 3 cut(s) 22, 1150, 1158
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 2 cut(s) 296, 1136
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 3 cut(s) 395, 676, 969
HpyCH4V TGCA 7 cut(s) 96, 1010, 1187, 1229, 1241, 1273, 1286
HpyF10VI GCNNNNNNNGC 1 cut(s) 980
HpyF3I CTNAG 6 cut(s) 40, 65, 204, 835, 1019, 1111
HpySE526I ACGT 3 cut(s) 395, 676, 969
Hsp92II CATG 5 cut(s) 752, 886, 982, 1178, 1221
Ksp22I TGATCA 1 cut(s) 745
KspAI GTTAAC 1 cut(s) 1150
Kzo9I GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
LpnPI CCDG 8 cut(s) 63, 427, 494, 521, 630, 705, 888, 1085
Lsp1109I GCAGC 3 cut(s) 220, 1253, 1298
LweI GCATC 5 cut(s) 105, 199, 997, 1019, 1238
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 3 cut(s) 335, 1037, 1127
MaeII ACGT 3 cut(s) 395, 676, 969
MaeIII GTNAC 2 cut(s) 556, 1121
MalI GATC 8 cut(s) 6, 333, 444, 489, 720, 747, 905, 1034
MboI GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
MflI RGATCY 4 cut(s) 4, 331, 487, 1032
MluCI AATT 4 cut(s) 275, 590, 803, 932
MlyI GAGTC 2 cut(s) 196, 821
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 5 cut(s) 35, 572, 597, 911, 1149
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 3 cut(s) 414, 875, 1072
MspR9I CCNGG 3 cut(s) 415, 509, 1072
MssI GTTTAAAC 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 980
NciI CCSGG 2 cut(s) 415, 1072
NcoI CCATGG 1 cut(s) 1217
NdeII GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
NlaIII CATG 5 cut(s) 752, 886, 982, 1178, 1221
NlaIV GGNNCC 3 cut(s) 6, 513, 1034
NsiI ATGCAT 1 cut(s) 1012
NspV TTCGAA 1 cut(s) 454
PagI TCATGA 1 cut(s) 748
PctI GAATGC 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 413
PkrI GCNGC 4 cut(s) 210, 1243, 1267, 1288
PleI GAGTC 2 cut(s) 196, 821
PmeI GTTTAAAC 1 cut(s) 573
PpsI GAGTC 2 cut(s) 196, 821
Ppu21I YACGTR 2 cut(s) 677, 970
PsiI TTATAA 1 cut(s) 992
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 3 cut(s) 6, 513, 1034
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 4 cut(s) 4, 331, 487, 1032
PvuII CAGCTG 1 cut(s) 77
SaqAI TTAA 5 cut(s) 35, 572, 597, 911, 1149
SatI GCNGC 4 cut(s) 209, 1242, 1266, 1287
Sau3AI GATC 8 cut(s) 4, 331, 442, 487, 718, 745, 903, 1032
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 2 cut(s) 196, 821
ScrFI CCNGG 3 cut(s) 415, 509, 1072
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 5 cut(s) 105, 199, 997, 1019, 1238
SfuI TTCGAA 1 cut(s) 454
SinI GGWCC 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 270, 1164
SmoI CTYRAG 2 cut(s) 270, 1164
SnaBI TACGTA 2 cut(s) 677, 970
Sse9I AATT 4 cut(s) 275, 590, 803, 932
SsiI CCGC 2 cut(s) 897, 1265
SspMI CTAG 3 cut(s) 335, 1037, 1127
StyD4I CCNGG 3 cut(s) 413, 507, 1070
StyI CCWWGG 1 cut(s) 1217
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 3 cut(s) 398, 679, 972
TasI AATT 4 cut(s) 275, 590, 803, 932
TauI GCSGC 1 cut(s) 1268
Tru1I TTAA 5 cut(s) 35, 572, 597, 911, 1149
Tru9I TTAA 5 cut(s) 35, 572, 597, 911, 1149
TseI GCWGC 3 cut(s) 208, 1241, 1286
TspDTI ATGAA 8 cut(s) 159, 765, 776, 936, 967, 1077, 1169, 1187
TspGWI ACGGA 2 cut(s) 687, 1292
VpaK11BI GGWCC 1 cut(s) 167
XagI CCTNNNNNAGG 1 cut(s) 1130
XapI RAATTY 2 cut(s) 590, 803
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
XmiI GTMKAC 2 cut(s) 21, 1157
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 335, 1037, 1127
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.