FvH4_1g13920

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002062
Physical Location & Seq
Reverse (-)
7663603 .. 7664651
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13920.t1

Sequence Viewer

Length: 678 bp
ATGGCGAATGGAGGAAGAGTGCTTGATGAGATTACAAGTCCAGCCAGAAACAATATTCAGACGACATTGTTGGAGCAAAGAATTGAGGAAACAAAATGGTTATTACATCCATCAGCTGGAAAAAGTTCTTGTTGCATCTTCAGAGTACCCCCATGCCTAGTGGAAATAAACAAGAACACCTACCGGCCTCGTATAGTCTCTCTCGGTCCTTATCACCATGGTGAAAAACATTTGGAGATGATGCAGCAGCACAAGTGGAGATTTCTTCATGATCTGCTTGCTCGGACACCGCTTACTGGTCCACGACTTGATGACTACTTGCAGGTTGTGTCATCAATGGAAGAGGACATAAGAGGGTGCTATTCAGAGACAATCAATTTTTGCAGCCTCGATCTCGTTGAAATGATGGTGTTGGATGGTCTCTTTGTTATTGAACTTTTCTGTAAAGTTGGAAGATTATCTCCAAGTGATCCGGATGATCCCATTTTCAACTTGGCATGGATATTCCCTAATCTCATTCAAGATCTTTTTCGCTTGGAGAACCAAATTCCTTTCTTGGTTCTTCAAACATTGTTTGACAAATCAAAACCTTCAAGACAAGAAAGTGACTCATCCCTTGGCAGACTTGCTCTAGAATTCTTTAACTACGCAGTTGAGAGACCTGATGAAGTCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

226

Amino Acids

25.95

Weight (kDa)

5.28

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 46 - 220 7.5e-53 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 1 cut(s) 290
AclWI GGATC 2 cut(s) 464, 473
AcsI RAATTY 2 cut(s) 546, 635
AcuI CTGAAG 1 cut(s) 124
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 116, 296
AgsI TTSAA 6 cut(s) 401, 434, 490, 521, 566, 594
AleI CACNNNNGTG 1 cut(s) 219
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw26I GTCTC 4 cut(s) 202, 362, 425, 652
AlwI GGATC 2 cut(s) 464, 473
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 3 cut(s) 244, 247, 384
ApoI RAATTY 2 cut(s) 546, 635
Asp700I GAANNNNTTC 1 cut(s) 124
AspS9I GGNCC 2 cut(s) 206, 299
AsuHPI GGTGA 2 cut(s) 206, 233
AvaII GGWCC 2 cut(s) 206, 299
BbvI GCAGC 3 cut(s) 256, 259, 396
BccI CCATC 3 cut(s) 118, 400, 410
BcoDI GTCTC 4 cut(s) 202, 362, 425, 652
BfaI CTAG 2 cut(s) 158, 632
BfuAI ACCTGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 523
BisI GCNGC 3 cut(s) 245, 248, 385
BlsI GCNGC 3 cut(s) 246, 249, 386
Bme18I GGWCC 2 cut(s) 206, 299
BmgT120I GGNCC 2 cut(s) 206, 299
BmsI GCATC 2 cut(s) 144, 231
BsaI GGTCTC 2 cut(s) 425, 652
BsaJI CCNNGG 2 cut(s) 217, 616
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 296
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 301
BseAI TCCGGA 1 cut(s) 472
BseDI CCNNGG 2 cut(s) 217, 616
BseGI GGATG 4 cut(s) 106, 421, 481, 611
BseLI CCNNNNNNNGG 2 cut(s) 116, 296
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 3 cut(s) 256, 259, 396
BshFI GGCC 1 cut(s) 187
BsiSI CCGG 2 cut(s) 184, 473
BslI CCNNNNNNNGG 2 cut(s) 116, 296
BsmAI GTCTC 4 cut(s) 202, 362, 425, 652
BsnI GGCC 1 cut(s) 187
Bso31I GGTCTC 2 cut(s) 425, 652
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 5 cut(s) 271, 391, 469, 478, 523
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 1 cut(s) 290
BspANI GGCC 1 cut(s) 187
BspEI TCCGGA 1 cut(s) 472
BspHI TCATGA 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 2 cut(s) 464, 473
BspTNI GGTCTC 2 cut(s) 425, 652
BsrFI RCCGGY 1 cut(s) 183
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 217, 616
BssMI GATC 5 cut(s) 271, 391, 469, 478, 523
BssT1I CCWWGG 2 cut(s) 217, 616
Bst6I CTCTTC 2 cut(s) 10, 336
BstC8I GCNNGC 1 cut(s) 279
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 4 cut(s) 106, 421, 481, 611
BstKTI GATC 5 cut(s) 274, 394, 472, 481, 526
BstMAI GTCTC 4 cut(s) 202, 362, 425, 652
BstMBI GATC 5 cut(s) 271, 391, 469, 478, 523
BstV1I GCAGC 3 cut(s) 256, 259, 396
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 4 cut(s) 106, 421, 481, 611
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 279
CciI TCATGA 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 206, 299
Csp6I GTAC 1 cut(s) 146
CviAII CATG 4 cut(s) 153, 218, 269, 498
CviJI RGCY 4 cut(s) 44, 116, 187, 387
CviKI_1 RGCY 4 cut(s) 44, 116, 187, 387
CviQI GTAC 1 cut(s) 146
DpnI GATC 5 cut(s) 273, 393, 471, 480, 525
DpnII GATC 5 cut(s) 271, 391, 469, 478, 523
Eam1104I CTCTTC 2 cut(s) 10, 336
EarI CTCTTC 2 cut(s) 10, 336
Eco130I CCWWGG 2 cut(s) 217, 616
Eco31I GGTCTC 2 cut(s) 425, 652
Eco47I GGWCC 2 cut(s) 206, 299
Eco57I CTGAAG 1 cut(s) 124
EcoRI GAATTC 1 cut(s) 635
EcoT14I CCWWGG 2 cut(s) 217, 616
ErhI CCWWGG 2 cut(s) 217, 616
FaeI CATG 4 cut(s) 156, 221, 272, 501
FaiI YATR 7 cut(s) 154, 194, 219, 270, 350, 499, 676
FatI CATG 4 cut(s) 152, 217, 268, 497
Fnu4HI GCNGC 3 cut(s) 245, 248, 385
FokI GGATG 4 cut(s) 93, 428, 488, 598
Fsp4HI GCNGC 3 cut(s) 245, 248, 385
FspBI CTAG 2 cut(s) 158, 632
GluI GCNGC 3 cut(s) 245, 248, 385
HaeIII GGCC 1 cut(s) 187
HapII CCGG 2 cut(s) 184, 473
Hin1II CATG 4 cut(s) 156, 221, 272, 501
HinfI GANTC 1 cut(s) 608
HpaII CCGG 2 cut(s) 184, 473
HphI GGTGA 2 cut(s) 206, 233
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 4 cut(s) 60, 143, 285, 367
Hpy188III TCNNGA 5 cut(s) 269, 473, 521, 594, 632
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 600
HpyCH4V TGCA 4 cut(s) 135, 244, 322, 384
Hsp92II CATG 4 cut(s) 156, 221, 272, 501
Kpn2I TCCGGA 1 cut(s) 472
Kzo9I GATC 5 cut(s) 271, 391, 469, 478, 523
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 7 cut(s) 54, 58, 102, 197, 282, 308, 486
Lsp1109I GCAGC 3 cut(s) 256, 259, 396
LweI GCATC 2 cut(s) 144, 231
MaeI CTAG 2 cut(s) 158, 632
MaeIII GTNAC 1 cut(s) 605
MalI GATC 5 cut(s) 273, 393, 471, 480, 525
MboI GATC 5 cut(s) 271, 391, 469, 478, 523
MboII GAAGA 6 cut(s) 27, 130, 257, 353, 465, 554
MflI RGATCY 1 cut(s) 523
MluCI AATT 4 cut(s) 81, 376, 546, 635
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 3 cut(s) 51, 393, 430
MnlI CCTC 6 cut(s) 5, 79, 198, 337, 347, 398
MroI TCCGGA 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 642
MslI CAYNNNNRTG 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 2 cut(s) 184, 473
NcoI CCATGG 1 cut(s) 217
NdeII GATC 5 cut(s) 271, 391, 469, 478, 523
NlaIII CATG 4 cut(s) 156, 221, 272, 501
NmuCI GTSAC 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 219
PagI TCATGA 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 124
PflMI CCANNNNNTGG 1 cut(s) 116
PkrI GCNGC 3 cut(s) 246, 249, 386
PleI GAGTC 1 cut(s) 602
PpsI GAGTC 1 cut(s) 602
PspPI GGNCC 2 cut(s) 206, 299
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 3 cut(s) 245, 248, 385
Sau3AI GATC 5 cut(s) 271, 391, 469, 478, 523
Sau96I GGNCC 2 cut(s) 206, 299
SchI GAGTC 1 cut(s) 602
SetI ASST 5 cut(s) 118, 182, 327, 592, 664
SfaNI GCATC 2 cut(s) 144, 231
SinI GGWCC 2 cut(s) 206, 299
SmiMI CAYNNNNRTG 1 cut(s) 219
Sse9I AATT 4 cut(s) 81, 376, 546, 635
SsiI CCGC 1 cut(s) 290
SspI AATATT 1 cut(s) 55
SspMI CTAG 2 cut(s) 158, 632
StyI CCWWGG 2 cut(s) 217, 616
TaqI TCGA 1 cut(s) 390
TaqII GACCGA 1 cut(s) 194
TasI AATT 4 cut(s) 81, 376, 546, 635
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 3 cut(s) 244, 247, 384
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 206, 299
XapI RAATTY 2 cut(s) 546, 635
XbaI TCTAGA 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 2 cut(s) 158, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.