FvH4_1g12920

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002062
Physical Location & Seq
Forward (+)
7143114 .. 7144469
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12920.t1

Sequence Viewer

Length: 1356 bp
ATGGCGAATGGAGGAAGAGGTGATGCTGTCATTACGATTGAAGCAGAGAAAACAAAGTGGCTACTTCACCCATTAGCCGGAAACGAGTCTTGTTGCATTTTCAGAGTTCCCCGGTGCCTTGCAGAAATACACCCCAAGACTTACCAGCCTCACATAGTCTCCATCGGCCCTTACCACTATGGAAACCAAGACCTTCAGATGATGCAGGAGCACAAACAGAGGTATCTTCGTGACCTGCTAGCTCGAACACAATCGACCGGTCCGAAACTTGATGACTATCTGCAGGCTGTTGCGTCTAGGGAACAAGACATAAGAGATTGCTACTCGGAGAACATCAAGTTCGGCAGCAGTGATCTAGTTGCGATAATGGTGCTGGACGGTCTCTTCATTATCGAACTGTTCTGCAGAGAGTTTGGACTAGTGTCACTACCACAGAGTCATGGTGACGATCCGATCTTTAACTTGGCATGGGTGAAGCCTAATCTCATACGAGATTTTCTCCGGTTGGAGAACCAAATCCCTTTCTTTGTTCTTCGAATCTTGTTTGAGAAATTCAAAGCATCAAGATTAGATATAGAGCTGTCACTCTCCAAGCTCGCTTTGCAGTTCATTAGTTACGCAGTAAGAAGACCAGAAGAAGTCTTGAACCAGCGTTTTAAAGCCAAACATTTGCTTGATTTAGTGGGGTTGAGCTTTGCACCTCAACCTCGTAATCATCCTTCTCATCCAACTCAAGACAAGAAGCTGAAACACACTCCTTCCTTGCTCCTATCAATCCCACCTGCAAGAACACCTATCAAAGGCATCAAGTCGAACGCATGGGAGGTGGTGAAAAATTTGCTAGCAGGACTTAAACCGAAAGCAGTGAAGGCATCAGAAGAAACACCTCTCGAAGGCGAGTCAGTCCAACTGATCAAATCAGCAAGAAAGCTATATAAGTCTGGAATTAAGTTCAAGACAGTGGCGGCAACGAGCTTCTTGGACATCAGATTTCGCAACGGAGTACTCAAAATTCCAAACATATTAATAGATGATCTTCTTACTGATTTATTTCTATACTTTGTTGCATTTGAACAGTACCATTACTGGCATTGCTCATCAAGGCACATAACAAGCTATGCAGCATTCATGAGTTTCTTAATCCGGACGCCTGAGGATGCGACATTTCTGCGCGATAAAGACATTATCGACAACTATCTTGGAACCGATGAGGAAGTTTGCAGTTTCTTTAGAAGCTTAGGTAAAGATGTGACATTCTATACTGGTGAAAGCCATCTATACGAGTTGTTCAAAGACGTGAACAAGTATCAGAGAAATGTTTGGCATGTGACATGGGGAGAGTTCAAATTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

452

Amino Acids

51.97

Weight (kDa)

8.84

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 33 - 448 4.9e-103 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
Acc36I ACCTGC 2 cut(s) 243, 790
AccB1I GGYRCC 1 cut(s) 114
AccII CGCG 1 cut(s) 1173
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 1 cut(s) 965
AclWI GGATC 1 cut(s) 443
AcsI RAATTY 4 cut(s) 551, 835, 1011, 1346
AcuI CTGAAG 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 1148
AfaI GTAC 2 cut(s) 1005, 1079
AfiI CCNNNNNNNGG 3 cut(s) 77, 800, 893
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 8 cut(s) 41, 556, 646, 955, 1073, 1291, 1345, 1351
AhlI ACTAGT 1 cut(s) 418
AjiI CACGTC 1 cut(s) 1297
AluBI AGCT 9 cut(s) 242, 580, 595, 693, 745, 931, 975, 1116, 1236
AluI AGCT 9 cut(s) 242, 580, 595, 693, 745, 931, 975, 1116, 1236
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 163, 386
AlwI GGATC 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 2 cut(s) 345, 1121
ApoI RAATTY 4 cut(s) 551, 835, 1011, 1346
AseI ATTAAT 1 cut(s) 1025
AsiGI ACCGGT 1 cut(s) 257
AspLEI GCGC 1 cut(s) 1173
AspS9I GGNCC 2 cut(s) 167, 260
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 6 cut(s) 32, 59, 455, 484, 841, 1277
AsuII TTCGAA 1 cut(s) 535
AsuNHI GCTAGC 2 cut(s) 238, 841
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 1152
BaeI ACNNNNGTAYC 2 cut(s) 206, 239
BanI GGYRCC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 703, 735
BbsI GAAGAC 1 cut(s) 634
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 357, 1133
BccI CCATC 2 cut(s) 170, 1281
BcgI CGANNNNNNTGC 4 cut(s) 352, 386, 1150, 1184
BclI TGATCA 1 cut(s) 912
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 163, 386
BcuI ACTAGT 1 cut(s) 418
BfaI CTAG 5 cut(s) 239, 297, 356, 419, 842
BfmI CTRYAG 2 cut(s) 281, 403
BfuAI ACCTGC 2 cut(s) 243, 790
BisI GCNGC 3 cut(s) 346, 966, 1122
BlsI GCNGC 3 cut(s) 347, 967, 1123
BmcAI AGTACT 1 cut(s) 1005
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 1297
BmgT120I GGNCC 2 cut(s) 167, 260
BmiI GGNNCC 2 cut(s) 116, 1204
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 6 cut(s) 13, 192, 569, 813, 881, 1147
BmtI GCTAGC 2 cut(s) 242, 845
BoxI GACNNNNGTC 1 cut(s) 421
BpiI GAAGAC 1 cut(s) 634
BplI GAGNNNNNCTC 2 cut(s) 483, 515
Bpu10I CCTNAGC 1 cut(s) 1237
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 717
BpuMI CCSGG 1 cut(s) 112
BsaBI GATNNNNATC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 1148
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 110
BsaWI WCCGGW 3 cut(s) 257, 501, 1143
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 800, 893
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 1091, 1267
Bse21I CCTNAGG 1 cut(s) 1152
Bse3DI GCAATG 1 cut(s) 1090
Bse8I GATNNNNATC 1 cut(s) 276
BseAI TCCGGA 1 cut(s) 1143
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 3 cut(s) 715, 724, 1162
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 3 cut(s) 77, 800, 893
BseMI GCAATG 1 cut(s) 1090
BseMII CTCAG 1 cut(s) 1143
BseNI ACTGG 2 cut(s) 1091, 1267
BseXI GCAGC 2 cut(s) 357, 1133
Bsh1236I CGCG 1 cut(s) 1173
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 114
BshTI ACCGGT 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 5 cut(s) 78, 112, 258, 502, 1144
BslI CCNNNNNNNGG 3 cut(s) 77, 800, 893
BsmAI GTCTC 2 cut(s) 163, 386
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 1143
Bsp143I GATC 5 cut(s) 352, 448, 453, 912, 1033
BspACI CCGC 1 cut(s) 965
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 1144
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 1 cut(s) 1173
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 2 cut(s) 116, 1204
BspMAI CTGCAG 2 cut(s) 285, 407
BspMI ACCTGC 2 cut(s) 243, 790
BspOI GCTAGC 2 cut(s) 242, 845
BspPI GGATC 1 cut(s) 443
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 1090
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 2 cut(s) 1091, 1267
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 5 cut(s) 352, 448, 453, 912, 1033
BssNI GRCGYC 1 cut(s) 1148
Bst4CI ACNGT 4 cut(s) 380, 399, 961, 1077
Bst6I CTCTTC 2 cut(s) 10, 389
BstACI GRCGYC 1 cut(s) 1148
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 240, 285, 597, 843
BstDEI CTNAG 2 cut(s) 1152, 1237
BstENI CCTNNNNNAGG 2 cut(s) 798, 891
BstF5I GGATG 3 cut(s) 715, 724, 1162
BstFNI CGCG 1 cut(s) 1173
BstHHI GCGC 1 cut(s) 1173
BstKTI GATC 5 cut(s) 355, 451, 456, 915, 1036
BstMAI GTCTC 2 cut(s) 163, 386
BstMBI GATC 5 cut(s) 352, 448, 453, 912, 1033
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 2 cut(s) 601, 869
BstNSI RCATGY 1 cut(s) 1328
BstPAI GACNNNNGTC 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 2 cut(s) 281, 403
BstUI CGCG 1 cut(s) 1173
BstV1I GCAGC 2 cut(s) 357, 1133
BstV2I GAAGAC 1 cut(s) 634
Bsu36I CCTNAGG 1 cut(s) 1152
BsuRI GGCC 1 cut(s) 168
BtrI CACGTC 1 cut(s) 1297
BtsCI GGATG 3 cut(s) 715, 724, 1162
BtsI GCAGTG 2 cut(s) 355, 870
BtsIMutI CAGTG 3 cut(s) 355, 870, 966
BveI ACCTGC 2 cut(s) 243, 790
Cac8I GCNNGC 4 cut(s) 240, 285, 597, 843
CciI TCATGA 1 cut(s) 1128
CfoI GCGC 1 cut(s) 1173
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 167, 260
CpoI CGGWCCG 1 cut(s) 260
CseI GACGC 2 cut(s) 282, 1156
Csp6I GTAC 2 cut(s) 1004, 1078
CspAI ACCGGT 1 cut(s) 257
CspI CGGWCCG 1 cut(s) 260
CviAII CATG 6 cut(s) 440, 468, 819, 1129, 1325, 1332
CviQI GTAC 2 cut(s) 1004, 1078
DdeI CTNAG 2 cut(s) 1152, 1237
DpnI GATC 5 cut(s) 354, 450, 455, 914, 1035
DpnII GATC 5 cut(s) 352, 448, 453, 912, 1033
DraI TTTAAA 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 10, 389
EarI CTCTTC 2 cut(s) 10, 389
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 1152
EcoNI CCTNNNNNAGG 2 cut(s) 798, 891
FaeI CATG 6 cut(s) 443, 471, 822, 1132, 1328, 1335
FatI CATG 6 cut(s) 439, 467, 818, 1128, 1324, 1331
FbaI TGATCA 1 cut(s) 912
Fnu4HI GCNGC 3 cut(s) 346, 966, 1122
FokI GGATG 3 cut(s) 702, 711, 1169
Fsp4HI GCNGC 3 cut(s) 346, 966, 1122
FspBI CTAG 5 cut(s) 239, 297, 356, 419, 842
GlaI GCGC 1 cut(s) 1172
GluI GCNGC 3 cut(s) 346, 966, 1122
HaeIII GGCC 1 cut(s) 168
HapII CCGG 5 cut(s) 78, 112, 258, 502, 1144
HgaI GACGC 2 cut(s) 282, 1156
HhaI GCGC 1 cut(s) 1173
Hin1I GRCGYC 1 cut(s) 1148
Hin1II CATG 6 cut(s) 443, 471, 822, 1132, 1328, 1335
Hin6I GCGC 1 cut(s) 1171
HinP1I GCGC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 1234
HinfI GANTC 4 cut(s) 86, 436, 537, 899
HpaII CCGG 5 cut(s) 78, 112, 258, 502, 1144
HphI GGTGA 6 cut(s) 32, 59, 455, 484, 841, 1277
Hpy166II GTNNAC 1 cut(s) 1300
Hpy188I TCNGA 8 cut(s) 104, 198, 264, 328, 453, 877, 989, 1311
Hpy188III TCNNGA 9 cut(s) 230, 564, 643, 734, 890, 942, 955, 1129, 1144
Hpy8I GTNNAC 1 cut(s) 1300
HpyAV CCTTC 5 cut(s) 203, 729, 768, 862, 887
HpyCH4III ACNGT 4 cut(s) 380, 399, 961, 1077
HpyCH4IV ACGT 1 cut(s) 1296
HpyF10VI GCNNNNNNNGC 2 cut(s) 601, 869
HpyF3I CTNAG 2 cut(s) 1152, 1237
HpySE526I ACGT 1 cut(s) 1296
Hsp92I GRCGYC 1 cut(s) 1148
Hsp92II CATG 6 cut(s) 443, 471, 822, 1132, 1328, 1335
HspAI GCGC 1 cut(s) 1171
Kpn2I TCCGGA 1 cut(s) 1143
Ksp22I TGATCA 1 cut(s) 912
Kzo9I GATC 5 cut(s) 352, 448, 453, 912, 1033
LmnI GCTCC 2 cut(s) 208, 771
Lsp1109I GCAGC 2 cut(s) 357, 1133
LweI GCATC 6 cut(s) 13, 192, 569, 813, 881, 1147
MaeI CTAG 5 cut(s) 239, 297, 356, 419, 842
MaeII ACGT 1 cut(s) 1296
MaeIII GTNAC 7 cut(s) 230, 423, 443, 582, 614, 1249, 1327
MalI GATC 5 cut(s) 354, 450, 455, 914, 1035
MboI GATC 5 cut(s) 352, 448, 453, 912, 1033
MboII GAAGA 8 cut(s) 27, 218, 376, 524, 639, 647, 890, 1028
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 5 cut(s) 551, 835, 945, 1011, 1346
MlyI GAGTC 3 cut(s) 95, 445, 908
MmeI TCCRAC 3 cut(s) 486, 752, 931
MroI TCCGGA 1 cut(s) 1143
MseI TTAA 6 cut(s) 459, 657, 852, 948, 1025, 1139
MspI CCGG 5 cut(s) 78, 112, 258, 502, 1144
MspR9I CCNGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 1124
MvnI CGCG 1 cut(s) 1173
MwoI GCNNNNNNNGC 2 cut(s) 601, 869
NciI CCSGG 1 cut(s) 112
NdeII GATC 5 cut(s) 352, 448, 453, 912, 1033
NheI GCTAGC 2 cut(s) 238, 841
NlaIII CATG 6 cut(s) 443, 471, 822, 1132, 1328, 1335
NlaIV GGNNCC 2 cut(s) 116, 1204
NmuCI GTSAC 6 cut(s) 230, 423, 443, 582, 1249, 1327
NspI RCATGY 1 cut(s) 1328
NspV TTCGAA 1 cut(s) 535
PagI TCATGA 1 cut(s) 1128
PaqCI CACCTGC 1 cut(s) 790
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 1124
PfeI GAWTC 1 cut(s) 537
PinAI ACCGGT 1 cut(s) 257
PkrI GCNGC 3 cut(s) 347, 967, 1123
PleI GAGTC 3 cut(s) 94, 444, 907
PpsI GAGTC 3 cut(s) 94, 444, 907
PshAI GACNNNNGTC 1 cut(s) 421
PshBI ATTAAT 1 cut(s) 1025
PspN4I GGNNCC 2 cut(s) 116, 1204
PspPI GGNCC 2 cut(s) 167, 260
PstI CTGCAG 2 cut(s) 285, 407
RsaI GTAC 2 cut(s) 1005, 1079
RsaNI GTAC 2 cut(s) 1004, 1078
Rsr2I CGGWCCG 1 cut(s) 260
RsrII CGGWCCG 1 cut(s) 260
SaqAI TTAA 6 cut(s) 459, 657, 852, 948, 1025, 1139
SatI GCNGC 3 cut(s) 346, 966, 1122
Sau3AI GATC 5 cut(s) 352, 448, 453, 912, 1033
Sau96I GGNCC 2 cut(s) 167, 260
ScaI AGTACT 1 cut(s) 1005
SchI GAGTC 3 cut(s) 95, 445, 908
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 6 cut(s) 13, 192, 569, 813, 881, 1147
SfcI CTRYAG 2 cut(s) 281, 403
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
SpeI ACTAGT 1 cut(s) 418
Sse9I AATT 5 cut(s) 551, 835, 945, 1011, 1346
SsiI CCGC 1 cut(s) 965
SspMI CTAG 5 cut(s) 239, 297, 356, 419, 842
StyD4I CCNGG 1 cut(s) 110
TaaI ACNGT 4 cut(s) 380, 399, 961, 1077
TaiI ACGT 1 cut(s) 1299
TaqI TCGA 7 cut(s) 244, 254, 393, 535, 812, 891, 1188
TasI AATT 5 cut(s) 551, 835, 945, 1011, 1346
TatI WGTACW 1 cut(s) 1003
TauI GCSGC 1 cut(s) 968
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 6 cut(s) 459, 657, 852, 948, 1025, 1139
Tru9I TTAA 6 cut(s) 459, 657, 852, 948, 1025, 1139
TscAI CASTG 3 cut(s) 355, 870, 966
TseFI GTSAC 6 cut(s) 230, 423, 443, 582, 1249, 1327
TseI GCWGC 2 cut(s) 345, 1121
Tsp45I GTSAC 6 cut(s) 230, 423, 443, 582, 1249, 1327
TspDTI ATGAA 3 cut(s) 376, 598, 1117
TspGWI ACGGA 1 cut(s) 1014
TspRI CASTG 3 cut(s) 355, 870, 966
VpaK11BI GGWCC 1 cut(s) 260
VspI ATTAAT 1 cut(s) 1025
XagI CCTNNNNNAGG 2 cut(s) 798, 891
XapI RAATTY 4 cut(s) 551, 835, 1011, 1346
XceI RCATGY 1 cut(s) 1328
XspI CTAG 5 cut(s) 239, 297, 356, 419, 842
ZrmI AGTACT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.