Rh2DG168000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002062
Physical Location & Seq
Forward (+)
14608496 .. 14610454
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG168000.1

Sequence Viewer

Length: 1332 bp
ATGTCGGTGAACGGAGATGGAAACTATTCTGTCATTACAGTTGCATCGCTGAAGGAAACAGTCAACGGAACAGAATGGCTCCTGGACCCCTCAGCTGGAAATGAGTCTTTTTGCATCTTCCGAGTACCCAAATGCCTAGTTGACGTAAACAAGAACACCTACGGGCCTCATATAGTCTCCATCGGTCCTTATCACCATGGTGAAAAACATTTGGAGATGATGCAGCAGCACAAGAGGAGATTTCTTCGCGATCTTCTTGCTCAAACACCACCAACTGGCCCGGGACTAGACGACTACCGGCAGGCTGTAGCATTAAGGGAAGAAGAGATAAGAGGGTGCTATTCAGAGTCGACGCTCAAGTTTTGCAAGGATCAGCTCGTTGACATGATGGTGTTGGATGGTCTCTTCATTATTGAACTCTTGTGCAAAATTGGAGGATTATCAAAAACGCCAATTGGAGATGACCCCATCTTTCACTTTGCATCGGTCTTTACTTATCTCATCCGAGATCTTCTTCGGTTGGAGAACCAGATTCCTTTCTTTGTTCTTCAAACATTATTCAACGCATCAAGAGATTCAAGACATGCAAGTGACTTATCCCTTGCCGAACTGGCTCTACAATTATTCGAATCCGCAGTAGAAATACCTTATGGAGATCATGATCAAGGAAAACATTTACTTGATTTACTTCGTTTGAGTTTTATCCACCCAAGCATGAGGCTCAAAGAAATCAACCTTGGATTTACCTCTTCATCTGTTCAGATTATCCAATCAGCAAAAAAGCTTCATCTAGCTGGAATAAAGTTCAAGGCAAGGCATGCGCAGAATGAGGCAATGAGCTTCCTAGATATCAGATTCTCAAATGGAGTGCTTGAAATCCCACTTATAACACTGGATGATCTTTGTACTGATCTATTACTGAACTTCATTGCATTTGAACAATGCTACTCTCATTCTCCAAGGCACATAACCACTTACGCTGCATTCATGAGTTGCCTAGTTCGTTCGCCTACGGACGCAGCATTTCTCTGCGAACATGATATTATTGAAAAATATATTGCCACCGATGAGGAAGTTGCAAATTTTTTCAGAAATATGGGGAAGGATGTGCCTTTCGATATTGGCCAGAGTTATCTACTGGAGTTGTTCAAGGATGTAAATGAGTACCAGAGGAATATGTGGCATGTCCGATGGGCGGGTTTCAGGTTGAAGTATTTTGATACACCATGGTCGTTCTTGTCTGCTTTAGCTGCTGTCATACTTTTACTCTTCACTGCAATTCAGACCTTCTTTGCTGTTTATGCATATGTGCTTCCACCAAAAGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

443

Amino Acids

50.47

Weight (kDa)

5.66

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 39 - 424 6.7e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
Acc16I TGCGCA 1 cut(s) 822
AccB7I CCANNNNNTGG 1 cut(s) 275
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 249
AciI CCGC 2 cut(s) 633, 1196
AclWI GGATC 1 cut(s) 378
AcoI YGGCCR 1 cut(s) 1123
AcsI RAATTY 1 cut(s) 1081
AcuI CTGAAG 1 cut(s) 71
AfaI GTAC 3 cut(s) 126, 907, 1166
AfiI CCNNNNNNNGG 3 cut(s) 95, 275, 1195
AjnI CCWGG 1 cut(s) 81
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
AleI CACNNNNGTG 1 cut(s) 198
AluBI AGCT 6 cut(s) 95, 376, 784, 794, 840, 1250
AluI AGCT 6 cut(s) 95, 376, 784, 794, 840, 1250
Alw26I GTCTC 2 cut(s) 181, 407
AlwI GGATC 1 cut(s) 378
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 3 cut(s) 164, 277, 1123
ApeKI GCWGC 5 cut(s) 223, 226, 980, 1019, 1250
ApoI RAATTY 1 cut(s) 1081
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 4 cut(s) 85, 164, 185, 278
AsuC2I CCSGG 2 cut(s) 281, 282
AsuHPI GGTGA 3 cut(s) 19, 185, 212
AsuII TTCGAA 1 cut(s) 627
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 2 cut(s) 85, 185
BalI TGGCCA 1 cut(s) 1125
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 5 cut(s) 235, 238, 967, 1031, 1237
BccI CCATC 6 cut(s) 11, 188, 382, 392, 476, 1185
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 83
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 2 cut(s) 281, 282
BcoDI GTCTC 2 cut(s) 181, 407
BfaI CTAG 5 cut(s) 137, 287, 791, 845, 998
BfmI CTRYAG 1 cut(s) 306
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 508
BisI GCNGC 5 cut(s) 224, 227, 981, 1020, 1251
BlsI GCNGC 5 cut(s) 225, 228, 982, 1021, 1252
Bme1390I CCNGG 3 cut(s) 83, 281, 282
Bme18I GGWCC 2 cut(s) 85, 185
BmeT110I CYCGRG 1 cut(s) 280
BmgT120I GGNCC 4 cut(s) 85, 164, 185, 278
BmiI GGNNCC 2 cut(s) 80, 87
BmrFI CCNGG 3 cut(s) 83, 281, 282
BmsI GCATC 5 cut(s) 53, 123, 210, 491, 575
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 91
Bpu14I TTCGAA 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 281, 282
BsaBI GATNNNNATC 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 5 cut(s) 196, 280, 736, 959, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 275, 1195
Bse118I RCCGGY 1 cut(s) 297
Bse1I ACTGG 4 cut(s) 280, 615, 897, 1143
Bse3DI GCAATG 2 cut(s) 840, 927
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 5 cut(s) 196, 280, 736, 959, 1226
BseGI GGATG 5 cut(s) 403, 501, 901, 1111, 1159
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 95, 275, 1195
BseMI GCAATG 2 cut(s) 840, 927
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 4 cut(s) 280, 615, 897, 1143
BseRI GAGGAG 1 cut(s) 250
BseXI GCAGC 5 cut(s) 235, 238, 967, 1031, 1237
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 3 cut(s) 166, 279, 1125
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 281, 298
BslFI GGGAC 1 cut(s) 297
BslI CCNNNNNNNGG 3 cut(s) 95, 275, 1195
BsmAI GTCTC 2 cut(s) 181, 407
BsmFI GGGAC 1 cut(s) 297
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 3 cut(s) 166, 279, 1125
Bso31I GGTCTC 1 cut(s) 407
BsoBI CYCGRG 1 cut(s) 280
Bsp119I TTCGAA 1 cut(s) 627
Bsp143I GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
Bsp19I CCATGG 2 cut(s) 196, 1226
Bsp68I TCGCGA 1 cut(s) 249
BspACI CCGC 2 cut(s) 633, 1196
BspANI GGCC 3 cut(s) 166, 279, 1125
BspCNI CTCAG 1 cut(s) 104
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 2 cut(s) 658, 987
BspLI GGNNCC 2 cut(s) 80, 87
BspPI GGATC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 407
BsrDI GCAATG 2 cut(s) 840, 927
BsrFI RCCGGY 1 cut(s) 297
BsrI ACTGG 4 cut(s) 280, 615, 897, 1143
BssAI RCCGGY 1 cut(s) 297
BssECI CCNNGG 5 cut(s) 196, 280, 736, 959, 1226
BssMI GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
BssT1I CCWWGG 4 cut(s) 196, 736, 959, 1226
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 2 cut(s) 40, 61
Bst6I CTCTTC 4 cut(s) 318, 410, 754, 1274
BstAPI GCANNNNNTGC 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 303, 819
BstDEI CTNAG 1 cut(s) 91
BstDSI CCRYGG 2 cut(s) 196, 1226
BstF5I GGATG 5 cut(s) 403, 501, 901, 1111, 1159
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 7 cut(s) 253, 373, 511, 658, 664, 901, 913
BstMAI GTCTC 2 cut(s) 181, 407
BstMBI GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
BstMWI GCNNNNNNNGC 4 cut(s) 611, 818, 1250, 1301
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 3 cut(s) 587, 821, 1187
BstSCI CCNGG 3 cut(s) 81, 279, 280
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 5 cut(s) 235, 238, 967, 1031, 1237
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BsuRI GGCC 3 cut(s) 166, 279, 1125
BtgI CCRYGG 2 cut(s) 196, 1226
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 5 cut(s) 403, 501, 901, 1111, 1159
BtsI GCAGTG 1 cut(s) 1272
BtsIMutI CAGTG 2 cut(s) 890, 1272
BtuMI TCGCGA 1 cut(s) 249
Cac8I GCNNGC 2 cut(s) 303, 819
CciI TCATGA 2 cut(s) 658, 987
CfoI GCGC 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 4 cut(s) 85, 164, 185, 278
Cfr9I CCCGGG 1 cut(s) 280
CseI GACGC 2 cut(s) 361, 1025
Csp6I GTAC 3 cut(s) 125, 906, 1165
CviQI GTAC 3 cut(s) 125, 906, 1165
DdeI CTNAG 1 cut(s) 91
DpnI GATC 7 cut(s) 252, 372, 510, 657, 663, 900, 912
DpnII GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
EaeI YGGCCR 1 cut(s) 1123
Eam1104I CTCTTC 4 cut(s) 318, 410, 754, 1274
EarI CTCTTC 4 cut(s) 318, 410, 754, 1274
Eco130I CCWWGG 4 cut(s) 196, 736, 959, 1226
Eco31I GGTCTC 1 cut(s) 407
Eco32I GATATC 1 cut(s) 850
Eco47I GGWCC 2 cut(s) 85, 185
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 81
EcoRV GATATC 1 cut(s) 850
EcoT14I CCWWGG 4 cut(s) 196, 736, 959, 1226
EcoT22I ATGCAT 1 cut(s) 1306
ErhI CCWWGG 4 cut(s) 196, 736, 959, 1226
FaqI GGGAC 1 cut(s) 297
FauI CCCGC 1 cut(s) 1189
FauNDI CATATG 1 cut(s) 1306
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 5 cut(s) 224, 227, 981, 1020, 1251
FokI GGATG 5 cut(s) 410, 488, 908, 1118, 1166
Fsp4HI GCNGC 5 cut(s) 224, 227, 981, 1020, 1251
FspBI CTAG 5 cut(s) 137, 287, 791, 845, 998
FspI TGCGCA 1 cut(s) 822
GlaI GCGC 1 cut(s) 822
GluI GCNGC 5 cut(s) 224, 227, 981, 1020, 1251
GsuI CTGGAG 1 cut(s) 1160
HaeIII GGCC 3 cut(s) 166, 279, 1125
HapII CCGG 2 cut(s) 281, 298
HgaI GACGC 2 cut(s) 361, 1025
HhaI GCGC 1 cut(s) 823
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 4 cut(s) 64, 142, 351, 382
HindII GTYRAC 4 cut(s) 64, 142, 351, 382
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 6 cut(s) 104, 347, 532, 575, 629, 855
HpaII CCGG 2 cut(s) 281, 298
HphI GGTGA 3 cut(s) 19, 185, 212
Hpy166II GTNNAC 6 cut(s) 10, 64, 142, 148, 351, 382
Hpy188I TCNGA 8 cut(s) 122, 346, 506, 762, 854, 1091, 1190, 1284
Hpy188III TCNNGA 5 cut(s) 248, 570, 579, 659, 988
Hpy8I GTNNAC 6 cut(s) 10, 64, 142, 148, 351, 382
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 46, 1096, 1297
HpyCH4III ACNGT 2 cut(s) 40, 61
HpyCH4IV ACGT 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 4 cut(s) 611, 818, 1250, 1301
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 1 cut(s) 144
HspAI GCGC 1 cut(s) 821
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 5 cut(s) 235, 238, 967, 1031, 1237
LweI GCATC 5 cut(s) 53, 123, 210, 491, 575
MaeI CTAG 5 cut(s) 137, 287, 791, 845, 998
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 590
MalI GATC 7 cut(s) 252, 372, 510, 657, 663, 900, 912
MboI GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 508
MlsI TGGCCA 1 cut(s) 1125
MluCI AATT 5 cut(s) 429, 453, 620, 1081, 1278
MluNI TGGCCA 1 cut(s) 1125
MlyI GAGTC 2 cut(s) 113, 356
MmeI TCCRAC 2 cut(s) 375, 501
Mox20I TGGCCA 1 cut(s) 1125
Mph1103I ATGCAT 1 cut(s) 1306
MroXI GAANNNNTTC 1 cut(s) 25
MscI TGGCCA 1 cut(s) 1125
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 3 cut(s) 198, 389, 588
Msp20I TGGCCA 1 cut(s) 1125
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 2 cut(s) 281, 298
MspR9I CCNGG 3 cut(s) 83, 281, 282
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 4 cut(s) 611, 818, 1250, 1301
NciI CCSGG 2 cut(s) 281, 282
NcoI CCATGG 2 cut(s) 196, 1226
NdeI CATATG 1 cut(s) 1306
NdeII GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
NlaIV GGNNCC 2 cut(s) 80, 87
NmuCI GTSAC 1 cut(s) 590
NruI TCGCGA 1 cut(s) 249
NsbI TGCGCA 1 cut(s) 822
NsiI ATGCAT 1 cut(s) 1306
NspI RCATGY 3 cut(s) 587, 821, 1187
NspV TTCGAA 1 cut(s) 627
OliI CACNNNNGTG 1 cut(s) 198
PaeI GCATGC 1 cut(s) 821
PagI TCATGA 2 cut(s) 658, 987
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 4 cut(s) 532, 575, 629, 855
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 5 cut(s) 225, 228, 982, 1021, 1252
PleI GAGTC 2 cut(s) 112, 355
PpsI GAGTC 2 cut(s) 112, 355
PsiI TTATAA 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 80, 87
PspPI GGNCC 4 cut(s) 85, 164, 185, 278
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PsuI RGATCY 1 cut(s) 508
PvuII CAGCTG 1 cut(s) 95
RruI TCGCGA 1 cut(s) 249
RsaI GTAC 3 cut(s) 126, 907, 1166
RsaNI GTAC 3 cut(s) 125, 906, 1165
RseI CAYNNNNRTG 3 cut(s) 198, 389, 588
SalI GTCGAC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 5 cut(s) 224, 227, 981, 1020, 1251
Sau3AI GATC 7 cut(s) 250, 370, 508, 655, 661, 898, 910
Sau96I GGNCC 4 cut(s) 85, 164, 185, 278
SchI GAGTC 2 cut(s) 113, 356
ScrFI CCNGG 3 cut(s) 83, 281, 282
SfaNI GCATC 5 cut(s) 53, 123, 210, 491, 575
SfcI CTRYAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 627
SinI GGWCC 2 cut(s) 85, 185
SmaI CCCGGG 1 cut(s) 282
SmiMI CAYNNNNRTG 3 cut(s) 198, 389, 588
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SphI GCATGC 1 cut(s) 821
Sse9I AATT 5 cut(s) 429, 453, 620, 1081, 1278
SsiI CCGC 2 cut(s) 633, 1196
SspMI CTAG 5 cut(s) 137, 287, 791, 845, 998
StyD4I CCNGG 3 cut(s) 81, 279, 280
StyI CCWWGG 4 cut(s) 196, 736, 959, 1226
TaaI ACNGT 2 cut(s) 40, 61
TaiI ACGT 1 cut(s) 147
TaqI TCGA 3 cut(s) 350, 627, 1116
TaqII GACCGA 2 cut(s) 173, 475
TasI AATT 5 cut(s) 429, 453, 620, 1081, 1278
TatI WGTACW 1 cut(s) 905
TfiI GAWTC 4 cut(s) 532, 575, 629, 855
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 2 cut(s) 897, 1279
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 5 cut(s) 223, 226, 980, 1019, 1250
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 5 cut(s) 397, 741, 776, 916, 976
TspGWI ACGGA 3 cut(s) 27, 81, 1028
TspMI CCCGGG 1 cut(s) 280
TspRI CASTG 2 cut(s) 897, 1279
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 85, 185
XapI RAATTY 1 cut(s) 1081
XceI RCATGY 3 cut(s) 587, 821, 1187
XmaI CCCGGG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 5 cut(s) 137, 287, 791, 845, 998
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.