AT5G22560

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002062
Physical Location & Seq
Reverse (-)
7491356 .. 7493269
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22560.1

Sequence Viewer

Length: 1554 bp
ATGAGAGCCATGTATCTTCCCAAGCAACACACTGGAGGAAGCACAACAATTTCAGGGGAAGATGATTGCATCGTACAAATCCGAAGGGTAGATGAAGATTCCAAAATACTTCTGAGAAATTCAGCAGCTCACCAATCTTGTTGCATCTTTAGAATCCCTCACACTCTCGTTCAATCCAATGATACAGCGTACAAACCGAAGATTGTCTCGATCGGTCCTTATCACCATGATCACGGTAAAGCTGATAAAGTCGATAAAGCCGATAAAGCGGATAAAGCACGTTTTAAGATGATCCAGCAGCACAAACAACGATATCTTGATATCTTCTTGTCCAAGACTACGAAAAAGGGCGTGGGCATAGAAGACTTGCAAAACGTGGTCTGGAAAAAGGAACATCTCATACGAGATTCCTACTCCGAGGAACTACAACTTAATCAAGTAGAGCTGATCGATCTCATGGTTCTTGATGGTTGCTTCATCCTTATGTTGTTCCTCTTGGTTTCACGTAAGGTTCCGCATAAAGCATTTGAGGATCCCATCTTCATGTTGAGGTGGATTTTACCAACTCTACGAAGTGATCTCCTCCTCCTTGAAAATCAGGTTCCGCTTTTCCTTCTTCGAGATCTTTTTAACACAACAAAGTTAGCTACTAAAACTAGCTTAAATGAGATGATCTTCAACTTTTTTGGCTATTCGATAAAAAGGCCACAAAAATTTTGGGATGAAAGAATGAATCTTGATGCAAGCCATCTTCTTGATCTCATCCGTAAGACTTTCGTACCAAGTCAACCTAAAAAAGCCGAAGGAGAAAGGTGGACCAATATGTTTTGCGGTTCCAGATGCTTCAATATGTTAAGTCCTTGGAAAAATAAAGTTCAAAGGGAGACATCAACACCACCACCAATAGAAACAAAAACACCACCACTACCACCACCACCACCAACACTAACACAACCACATCCAAAACCACTAACACCGCCTCCTCGCCGTTTCCTCAAGTTGGTTGTCTCAGCTAGAAAACTTCGACTCCGAGGAATAAAATTCCAACAAAAGAAGAAATTTGAGACACCTCTTGACATAACTCTCAAGAACGGTGTACTTAAAATACCGCCACTACTATTTGATGACTTTTTCAGCTCGCTTTTAATCAACTGCGTCGCCTTTGAGCAATTTAACGTGCAAGGCACGACCGAAATGACGAGCTATGTTACTTTCATGGGCTGCCTCATAAACACGGCAGATGATGCAACGTTCTTAAGCGAGAAAGGTATCATAGAGAACTATTTTGGAACGGGAGAACAATTATCTGTGTTCTTCAAGAATACAGGTAAAGACATTTCGTTCACAATATCGAAAAGTTACTTGGCAAATGTGTTCGAGGGAGTCAACAAGTACACTTCACAAGGATACCATGTACACTGGGCAGGAGTCAAGTACACATATTTCAAAAGTCCGTGGACATTTCTATCATCTTTTGCTGCTTTGGTACTTATTCTGCTTACCATTTTCCAAGCATTCTTCGCAGGCTACGCGTATTTTCGCCCTCCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

517

Amino Acids

59.53

Weight (kDa)

9.44

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 500 3.2e-105 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1532
AciI CCGC 6 cut(s) 269, 515, 605, 831, 977, 1109
AclI AACGTT 1 cut(s) 1250
AclWI GGATC 3 cut(s) 286, 527, 540
AcsI RAATTY 4 cut(s) 118, 713, 1040, 1058
AfaI GTAC 8 cut(s) 75, 191, 780, 1098, 1394, 1416, 1436, 1488
AfiI CCNNNNNNNGG 1 cut(s) 1000
AflII CTTAAG 1 cut(s) 1255
AflIII ACRYGT 1 cut(s) 1530
AgsI TTSAA 7 cut(s) 173, 593, 679, 847, 878, 1318, 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 1346, 1378
AluBI AGCT 8 cut(s) 128, 242, 445, 647, 660, 1013, 1137, 1203
AluI AGCT 8 cut(s) 128, 242, 445, 647, 660, 1013, 1137, 1203
Alw26I GTCTC 4 cut(s) 211, 878, 1012, 1058
AlwI GGATC 3 cut(s) 286, 527, 540
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 4 cut(s) 125, 298, 1221, 1478
ApoI RAATTY 4 cut(s) 118, 713, 1040, 1058
AspS9I GGNCC 2 cut(s) 215, 816
AsuHPI GGTGA 2 cut(s) 122, 215
AvaII GGWCC 2 cut(s) 215, 816
BaeI ACNNNNGTAYC 2 cut(s) 762, 795
BamHI GGATCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 4 cut(s) 137, 310, 1208, 1465
BccI CCATC 3 cut(s) 461, 545, 756
BceAI ACGGC 2 cut(s) 972, 1251
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 4 cut(s) 211, 878, 1012, 1058
BfaI CTAG 2 cut(s) 657, 1014
BfrI CTTAAG 1 cut(s) 1255
BfuI GTATCC 1 cut(s) 1400
BglII AGATCT 1 cut(s) 622
BisI GCNGC 4 cut(s) 126, 299, 1222, 1479
BlsI GCNGC 4 cut(s) 127, 300, 1223, 1480
Bme18I GGWCC 2 cut(s) 215, 816
BmgT120I GGNCC 2 cut(s) 215, 816
BmiI GGNNCC 4 cut(s) 513, 534, 603, 835
BmrI ACTGGG 1 cut(s) 1429
BmsI GCATC 5 cut(s) 78, 153, 730, 830, 1234
BmuI ACTGGG 1 cut(s) 1429
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 980, 1070
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 506
BsaJI CCNNGG 4 cut(s) 417, 860, 1030, 1454
BsaXI ACNNNNNCTCC 4 cut(s) 964, 994, 1011, 1041
Bsc4I CCNNNNNNNGG 1 cut(s) 1000
Bse1I ACTGG 2 cut(s) 37, 1424
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 4 cut(s) 417, 860, 1030, 1454
BseGI GGATG 4 cut(s) 477, 727, 762, 958
BseLI CCNNNNNNNGG 1 cut(s) 1000
BseMII CTCAG 2 cut(s) 104, 1023
BseNI ACTGG 2 cut(s) 37, 1424
BseRI GAGGAG 3 cut(s) 572, 575, 972
BseXI GCAGC 4 cut(s) 137, 310, 1208, 1465
Bsh1236I CGCG 1 cut(s) 1532
Bsh1285I CGRYCG 2 cut(s) 213, 1191
BshFI GGCC 1 cut(s) 706
BshVI ATCGAT 1 cut(s) 450
BsiEI CGRYCG 2 cut(s) 213, 1191
BslI CCNNNNNNNGG 1 cut(s) 1000
BsmAI GTCTC 4 cut(s) 211, 878, 1012, 1058
BsmI GAATGC 1 cut(s) 1514
BsnI GGCC 1 cut(s) 706
Bsp1407I TGTACA 1 cut(s) 1414
BspACI CCGC 6 cut(s) 269, 515, 605, 831, 977, 1109
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 2 cut(s) 105, 1022
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 1 cut(s) 1532
BspLI GGNNCC 4 cut(s) 513, 534, 603, 835
BspPI GGATC 3 cut(s) 286, 527, 540
BspTI CTTAAG 1 cut(s) 1255
BsrGI TGTACA 1 cut(s) 1414
BsrI ACTGG 2 cut(s) 37, 1424
BssECI CCNNGG 4 cut(s) 417, 860, 1030, 1454
BssT1I CCWWGG 1 cut(s) 860
Bst4CI ACNGT 2 cut(s) 236, 1094
BstAFI CTTAAG 1 cut(s) 1255
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstAUI TGTACA 1 cut(s) 1414
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 745, 1139, 1525
BstDEI CTNAG 2 cut(s) 113, 1009
BstDSI CCRYGG 1 cut(s) 1454
BstF5I GGATG 4 cut(s) 477, 727, 762, 958
BstFNI CGCG 1 cut(s) 1532
BstMAI GTCTC 4 cut(s) 211, 878, 1012, 1058
BstMCI CGRYCG 2 cut(s) 213, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 266, 275, 1244, 1520, 1529
BstUI CGCG 1 cut(s) 1532
BstV1I GCAGC 4 cut(s) 137, 310, 1208, 1465
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 532, 622
BstYI RGATCY 2 cut(s) 532, 622
Bsu15I ATCGAT 1 cut(s) 450
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 1 cut(s) 706
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 1454
BtsCI GGATG 4 cut(s) 477, 727, 762, 958
BtsIMutI CAGTG 2 cut(s) 30, 1417
Cac8I GCNNGC 3 cut(s) 745, 1139, 1525
Cfr13I GGNCC 2 cut(s) 215, 816
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 1 cut(s) 1144
Csp6I GTAC 8 cut(s) 74, 190, 779, 1097, 1393, 1415, 1435, 1487
CviAII CATG 6 cut(s) 10, 227, 457, 544, 1216, 1412
CviQI GTAC 8 cut(s) 74, 190, 779, 1097, 1393, 1415, 1435, 1487
DdeI CTNAG 2 cut(s) 113, 1009
Eco130I CCWWGG 1 cut(s) 860
Eco32I GATATC 2 cut(s) 314, 322
Eco47I GGWCC 2 cut(s) 215, 816
EcoRV GATATC 2 cut(s) 314, 322
EcoT14I CCWWGG 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 860
FaeI CATG 6 cut(s) 13, 230, 460, 547, 1219, 1415
FatI CATG 6 cut(s) 9, 226, 456, 543, 1215, 1411
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 4 cut(s) 126, 299, 1222, 1479
FokI GGATG 4 cut(s) 464, 734, 749, 945
Fsp4HI GCNGC 4 cut(s) 126, 299, 1222, 1479
FspBI CTAG 2 cut(s) 657, 1014
GluI GCNGC 4 cut(s) 126, 299, 1222, 1479
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 706
HgaI GACGC 1 cut(s) 1144
Hin1II CATG 6 cut(s) 13, 230, 460, 547, 1219, 1415
HincII GTYRAC 2 cut(s) 788, 1387
HindII GTYRAC 2 cut(s) 788, 1387
HinfI GANTC 7 cut(s) 98, 153, 407, 733, 1026, 1383, 1428
HphI GGTGA 2 cut(s) 122, 215
Hpy166II GTNNAC 9 cut(s) 788, 816, 1097, 1344, 1387, 1395, 1417, 1437, 1458
Hpy188I TCNGA 4 cut(s) 83, 114, 418, 1031
Hpy8I GTNNAC 9 cut(s) 788, 816, 1097, 1344, 1387, 1395, 1417, 1437, 1458
Hpy99I CGWCG 1 cut(s) 1160
HpyAV CCTTC 3 cut(s) 78, 623, 797
HpyCH4III ACNGT 2 cut(s) 236, 1094
HpyCH4IV ACGT 5 cut(s) 280, 375, 505, 1176, 1250
HpyCH4V TGCA 6 cut(s) 69, 144, 370, 743, 1180, 1247
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 275, 1244, 1520, 1529
HpyF3I CTNAG 2 cut(s) 113, 1009
HpySE526I ACGT 5 cut(s) 280, 375, 505, 1176, 1250
Hsp92II CATG 6 cut(s) 13, 230, 460, 547, 1219, 1415
Ksp22I TGATCA 1 cut(s) 229
Lsp1109I GCAGC 4 cut(s) 137, 310, 1208, 1465
LweI GCATC 5 cut(s) 78, 153, 730, 830, 1234
MaeI CTAG 2 cut(s) 657, 1014
MaeII ACGT 5 cut(s) 280, 375, 505, 1176, 1250
MaeIII GTNAC 2 cut(s) 1207, 1358
MflI RGATCY 2 cut(s) 532, 622
MluCI AATT 7 cut(s) 48, 118, 713, 1040, 1058, 1169, 1301
MluI ACGCGT 1 cut(s) 1530
MlyI GAGTC 3 cut(s) 1020, 1392, 1437
MmeI TCCRAC 1 cut(s) 1069
MseI TTAA 9 cut(s) 285, 432, 630, 662, 854, 1101, 1145, 1173, 1256
MslI CAYNNNNRTG 2 cut(s) 482, 542
MspCI CTTAAG 1 cut(s) 1255
Mva1269I GAATGC 1 cut(s) 1514
MvnI CGCG 1 cut(s) 1532
MwoI GCNNNNNNNGC 5 cut(s) 266, 275, 1244, 1520, 1529
NlaIII CATG 6 cut(s) 13, 230, 460, 547, 1219, 1415
NlaIV GGNNCC 4 cut(s) 513, 534, 603, 835
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 1514
PfeI GAWTC 4 cut(s) 98, 153, 407, 733
PkrI GCNGC 4 cut(s) 127, 300, 1223, 1480
Ple19I CGATCG 1 cut(s) 213
PleI GAGTC 3 cut(s) 1020, 1391, 1436
PpsI GAGTC 3 cut(s) 1020, 1391, 1436
Ppu21I YACGTR 1 cut(s) 506
Psp1406I AACGTT 1 cut(s) 1250
PspN4I GGNNCC 4 cut(s) 513, 534, 603, 835
PspPI GGNCC 2 cut(s) 215, 816
PsrI GAACNNNNNNTAC 2 cut(s) 384, 416
PsuI RGATCY 2 cut(s) 532, 622
PvuI CGATCG 1 cut(s) 213
RsaI GTAC 8 cut(s) 75, 191, 780, 1098, 1394, 1416, 1436, 1488
RsaNI GTAC 8 cut(s) 74, 190, 779, 1097, 1393, 1415, 1435, 1487
RseI CAYNNNNRTG 2 cut(s) 482, 542
SaqAI TTAA 9 cut(s) 285, 432, 630, 662, 854, 1101, 1145, 1173, 1256
SatI GCNGC 4 cut(s) 126, 299, 1222, 1479
Sau96I GGNCC 2 cut(s) 215, 816
SchI GAGTC 3 cut(s) 1020, 1392, 1437
SfaNI GCATC 5 cut(s) 78, 153, 730, 830, 1234
SinI GGWCC 2 cut(s) 215, 816
SmiMI CAYNNNNRTG 2 cut(s) 482, 542
SmlI CTYRAG 3 cut(s) 995, 1085, 1255
SmoI CTYRAG 3 cut(s) 995, 1085, 1255
Sse9I AATT 7 cut(s) 48, 118, 713, 1040, 1058, 1169, 1301
SsiI CCGC 6 cut(s) 269, 515, 605, 831, 977, 1109
SspMI CTAG 2 cut(s) 657, 1014
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 2 cut(s) 236, 1094
TaiI ACGT 5 cut(s) 283, 378, 508, 1179, 1253
TaqI TCGA 8 cut(s) 209, 252, 450, 619, 695, 1024, 1352, 1377
TaqII GACCGA 2 cut(s) 203, 1205
TasI AATT 7 cut(s) 48, 118, 713, 1040, 1058, 1169, 1301
TatI WGTACW 4 cut(s) 1096, 1392, 1414, 1434
TfiI GAWTC 4 cut(s) 98, 153, 407, 733
Tru1I TTAA 9 cut(s) 285, 432, 630, 662, 854, 1101, 1145, 1173, 1256
Tru9I TTAA 9 cut(s) 285, 432, 630, 662, 854, 1101, 1145, 1173, 1256
TscAI CASTG 2 cut(s) 37, 1424
TseI GCWGC 4 cut(s) 125, 298, 1221, 1478
TspDTI ATGAA 6 cut(s) 108, 466, 532, 738, 746, 1204
TspGWI ACGGA 2 cut(s) 755, 1443
TspRI CASTG 2 cut(s) 37, 1424
Vha464I CTTAAG 1 cut(s) 1255
VpaK11BI GGWCC 2 cut(s) 215, 816
XapI RAATTY 4 cut(s) 118, 713, 1040, 1058
XcmI CCANNNNNNNNNTGG 1 cut(s) 714
XspI CTAG 2 cut(s) 657, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.