RLG00000017223

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002062
Physical Location & Seq
Forward (+)
14288262 .. 14289617
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017223

Sequence Viewer

Length: 1077 bp
ATGGCTTCGTCCTCGAGCTCACCAATGTCGGTGAACGGAGATGGAAACTACTCTGTCATTACAGTTGCATCACTGGAGGAAACAGTCAACGGAACAGAATGGCTCCTGGACCCGGATCAGCTCGTTGACATGATGGTGTTGGATGGTCTCTTCATTATTGAACTCTTGTGCAAAATTGGAGGATTATCAAAAACGCCAATTGGAGATGACCCCATCTTTCACTTTGCATCGGTCTTTACTTATCTCATCCGAGATCTTCTTCGGTTGGAGAACCAGATTCCTTTCTTTGTTCTTCAAACATTATTCAACGCATCAAGAGATTCAAGACATGACAGTGACTCATCCCTTGCCACACTGGCTCTACAATTATTTGAATCCACAGTAGAAATACCTTATGGAGATCATGATCAAGGAAAACATTTACTTGATTTACTTCGTTTGAGTTTTATCCGCCCAAGCATGAGGCTCAAAGAAATCAACCTTGGATTTACCTCTTCATCTGTTCAGATTATCCAATCAGCAAAAAAGCTTCATCTAGCTGGAATAAAGTTCAAGGCAAGGCATGCGCAGAATGAGGCAATGAGCTTCCTAGATATCAGATTCTCAAATGGAGTGCTTGAAATCCCACTTATAACACTGGATGATCTTTGTACTGATCTATTACTGAACTTCGTTGCATTTGAACAATGCTACTCTCATTCTCCAAGGCACATAACCACTTACGCTGCATTCATGAGTTGCCTAGTTCGTTCGCCTACGGACGCAGCATTTCTCTGCGAACATGATATTATTGAAAAATATATTGGCACCGATGAGGAAGTTTCAAATTTTTTCAGAAACATGGGGAAGGATGTGCCTTTCGATATTGGCCAGAGTTATCTACTGGAGTTGTTCAAGGATGTAAATGAGTACCAGAGGAATATGTGGCACGTCCGATGGGCGGGTTTCAGGTTGAAGTATTTTGATACACCATGGTCTTTCTTGTCTGCTTTAGCTGCTGTCATACTCTTACTCTTCACTGCAATTCAGACCTTCTTTGCTGTTTATGCATATGTGCTTCCACCAAAAGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

359

Amino Acids

40.59

Weight (kDa)

5.0

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 38 - 339 1.2e-84 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
Acc16I TGCGCA 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 806
AciI CCGC 2 cut(s) 451, 941
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 1 cut(s) 826
AfaI GTAC 2 cut(s) 652, 911
AfiI CCNNNNNNNGG 2 cut(s) 112, 940
AjiI CACGTC 1 cut(s) 931
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 786, 818
AluBI AGCT 6 cut(s) 18, 121, 529, 539, 585, 995
AluI AGCT 6 cut(s) 18, 121, 529, 539, 585, 995
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 1 cut(s) 868
ApeKI GCWGC 3 cut(s) 725, 764, 995
ApoI RAATTY 1 cut(s) 826
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 12, 43
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 870
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 712, 776, 982
BccI CCATC 5 cut(s) 35, 127, 137, 221, 930
BciT130I CCWGG 1 cut(s) 107
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 3 cut(s) 536, 590, 743
BglI GCCNNNNNGGC 1 cut(s) 356
BglII AGATCT 1 cut(s) 253
BisI GCNGC 3 cut(s) 726, 765, 996
BlsI GCNGC 3 cut(s) 727, 766, 997
Bme1390I CCNGG 2 cut(s) 107, 113
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 931
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 3 cut(s) 104, 111, 808
BmrFI CCNGG 2 cut(s) 107, 113
BmsI GCATC 3 cut(s) 77, 236, 320
BpmI CTGGAG 2 cut(s) 95, 905
BpuMI CCSGG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 481, 704, 971
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 940
Bse1I ACTGG 4 cut(s) 78, 360, 642, 888
Bse3DI GCAATG 1 cut(s) 585
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 481, 704, 971
BseGI GGATG 6 cut(s) 148, 246, 341, 646, 856, 904
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 112, 940
BseMI GCAATG 1 cut(s) 585
BseNI ACTGG 4 cut(s) 78, 360, 642, 888
BseXI GCAGC 3 cut(s) 712, 776, 982
BshFI GGCC 1 cut(s) 870
BshNI GGYRCC 1 cut(s) 806
BsiHKAI GWGCWC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 112, 940
BsmAI GTCTC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 1 cut(s) 870
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 6 cut(s) 115, 253, 400, 406, 643, 655
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 2 cut(s) 451, 941
BspANI GGCC 1 cut(s) 870
BspHI TCATGA 2 cut(s) 403, 732
BspLI GGNNCC 3 cut(s) 104, 111, 808
BspPI GGATC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 806
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 4 cut(s) 78, 360, 642, 888
BssECI CCNNGG 3 cut(s) 481, 704, 971
BssMI GATC 6 cut(s) 115, 253, 400, 406, 643, 655
BssT1I CCWWGG 3 cut(s) 481, 704, 971
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 4 cut(s) 64, 85, 335, 382
Bst6I CTCTTC 3 cut(s) 155, 499, 1019
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 564
BstDSI CCRYGG 1 cut(s) 971
BstF5I GGATG 6 cut(s) 148, 246, 341, 646, 856, 904
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 6 cut(s) 118, 256, 403, 409, 646, 658
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 6 cut(s) 115, 253, 400, 406, 643, 655
BstMWI GCNNNNNNNGC 4 cut(s) 356, 563, 995, 1046
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 105, 111
BstV1I GCAGC 3 cut(s) 712, 776, 982
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 870
BtgI CCRYGG 1 cut(s) 971
BtrI CACGTC 1 cut(s) 931
BtsCI GGATG 6 cut(s) 148, 246, 341, 646, 856, 904
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 5 cut(s) 71, 340, 353, 635, 1017
Cac8I GCNNGC 1 cut(s) 564
CciI TCATGA 2 cut(s) 403, 732
CfoI GCGC 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 770
Csp6I GTAC 2 cut(s) 651, 910
CviAII CATG 9 cut(s) 130, 329, 404, 460, 563, 733, 782, 841, 972
CviQI GTAC 2 cut(s) 651, 910
DpnI GATC 6 cut(s) 117, 255, 402, 408, 645, 657
DpnII GATC 6 cut(s) 115, 253, 400, 406, 643, 655
EaeI YGGCCR 1 cut(s) 868
Eam1104I CTCTTC 3 cut(s) 155, 499, 1019
EarI CTCTTC 3 cut(s) 155, 499, 1019
EciI GGCGGA 1 cut(s) 440
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 3 cut(s) 481, 704, 971
Eco24I GRGCYC 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 105
EcoRV GATATC 1 cut(s) 595
EcoT14I CCWWGG 3 cut(s) 481, 704, 971
EcoT22I ATGCAT 1 cut(s) 1051
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 3 cut(s) 481, 704, 971
FaeI CATG 9 cut(s) 133, 332, 407, 463, 566, 736, 785, 844, 975
FatI CATG 9 cut(s) 129, 328, 403, 459, 562, 732, 781, 840, 971
FauI CCCGC 1 cut(s) 934
FauNDI CATATG 1 cut(s) 1051
FbaI TGATCA 1 cut(s) 406
Fnu4HI GCNGC 3 cut(s) 726, 765, 996
FokI GGATG 6 cut(s) 155, 233, 328, 653, 863, 911
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 726, 765, 996
FspBI CTAG 3 cut(s) 536, 590, 743
FspI TGCGCA 1 cut(s) 567
GlaI GCGC 1 cut(s) 567
GluI GCNGC 3 cut(s) 726, 765, 996
GsuI CTGGAG 2 cut(s) 95, 905
HaeIII GGCC 1 cut(s) 870
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 770
HhaI GCGC 1 cut(s) 568
Hin1II CATG 9 cut(s) 133, 332, 407, 463, 566, 736, 785, 844, 975
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 2 cut(s) 88, 127
HindII GTYRAC 2 cut(s) 88, 127
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 5 cut(s) 277, 320, 338, 374, 600
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 12, 43
Hpy166II GTNNAC 3 cut(s) 34, 88, 127
Hpy188I TCNGA 6 cut(s) 251, 507, 599, 836, 935, 1029
Hpy188III TCNNGA 4 cut(s) 315, 324, 404, 733
Hpy8I GTNNAC 3 cut(s) 34, 88, 127
HpyAV CCTTC 2 cut(s) 841, 1042
HpyCH4III ACNGT 4 cut(s) 64, 85, 335, 382
HpyCH4IV ACGT 1 cut(s) 930
HpyCH4V TGCA 7 cut(s) 68, 171, 227, 677, 728, 1022, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 563, 995, 1046
HpySE526I ACGT 1 cut(s) 930
Hsp92II CATG 9 cut(s) 133, 332, 407, 463, 566, 736, 785, 844, 975
HspAI GCGC 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 6 cut(s) 115, 253, 400, 406, 643, 655
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 3 cut(s) 712, 776, 982
LweI GCATC 3 cut(s) 77, 236, 320
MaeI CTAG 3 cut(s) 536, 590, 743
MaeII ACGT 1 cut(s) 930
MaeIII GTNAC 1 cut(s) 335
MalI GATC 6 cut(s) 117, 255, 402, 408, 645, 657
MboI GATC 6 cut(s) 115, 253, 400, 406, 643, 655
MboII GAAGA 6 cut(s) 142, 248, 251, 284, 486, 1006
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 20
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 5 cut(s) 174, 198, 365, 826, 1023
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 120, 246
MnlI CCTC 8 cut(s) 22, 70, 173, 456, 502, 568, 808, 909
Mox20I TGGCCA 1 cut(s) 870
Mph1103I ATGCAT 1 cut(s) 1051
MscI TGGCCA 1 cut(s) 870
MslI CAYNNNNRTG 2 cut(s) 134, 333
Msp20I TGGCCA 1 cut(s) 870
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 2 cut(s) 107, 113
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 728
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 356, 563, 995, 1046
NciI CCSGG 1 cut(s) 113
NcoI CCATGG 1 cut(s) 971
NdeI CATATG 1 cut(s) 1051
NdeII GATC 6 cut(s) 115, 253, 400, 406, 643, 655
NlaIII CATG 9 cut(s) 133, 332, 407, 463, 566, 736, 785, 844, 975
NlaIV GGNNCC 3 cut(s) 104, 111, 808
NmuCI GTSAC 1 cut(s) 335
NsbI TGCGCA 1 cut(s) 567
NsiI ATGCAT 1 cut(s) 1051
NspI RCATGY 1 cut(s) 566
PaeI GCATGC 1 cut(s) 566
PaeR7I CTCGAG 1 cut(s) 13
PagI TCATGA 2 cut(s) 403, 732
PctI GAATGC 1 cut(s) 728
PfeI GAWTC 4 cut(s) 277, 320, 374, 600
PfoI TCCNGGA 1 cut(s) 105
PkrI GCNGC 3 cut(s) 727, 766, 997
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PsiI TTATAA 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 3 cut(s) 104, 111, 808
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 13
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 2 cut(s) 652, 911
RsaNI GTAC 2 cut(s) 651, 910
RseI CAYNNNNRTG 2 cut(s) 134, 333
SacI GAGCTC 1 cut(s) 20
SatI GCNGC 3 cut(s) 726, 765, 996
Sau3AI GATC 6 cut(s) 115, 253, 400, 406, 643, 655
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 107, 113
SduI GDGCHC 1 cut(s) 20
SfaNI GCATC 3 cut(s) 77, 236, 320
Sfr274I CTCGAG 1 cut(s) 13
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 134, 333
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
SphI GCATGC 1 cut(s) 566
Sse9I AATT 5 cut(s) 174, 198, 365, 826, 1023
SsiI CCGC 2 cut(s) 451, 941
SspMI CTAG 3 cut(s) 536, 590, 743
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 105, 111
StyI CCWWGG 3 cut(s) 481, 704, 971
TaaI ACNGT 4 cut(s) 64, 85, 335, 382
TaiI ACGT 1 cut(s) 933
TaqI TCGA 2 cut(s) 14, 861
TaqII GACCGA 1 cut(s) 220
TasI AATT 5 cut(s) 174, 198, 365, 826, 1023
TatI WGTACW 1 cut(s) 650
TfiI GAWTC 4 cut(s) 277, 320, 374, 600
TscAI CASTG 5 cut(s) 78, 340, 360, 642, 1024
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 3 cut(s) 725, 764, 995
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 4 cut(s) 142, 486, 521, 721
TspGWI ACGGA 3 cut(s) 51, 105, 773
TspRI CASTG 5 cut(s) 78, 340, 360, 642, 1024
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 826
XceI RCATGY 1 cut(s) 566
XhoI CTCGAG 1 cut(s) 13
XspI CTAG 3 cut(s) 536, 590, 743
Zsp2I ATGCAT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.