MD10G1146500.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002062
Physical Location & Seq
Reverse (-)
23294151 .. 23295584
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1146500.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGACCTCACCATCAACATCAAGCTCAAGTTTATCAACAGCAAACAATGGAGGTAGAGACGGGGATGTGGTCATTCAAATGGCATCCCCGGACGCCATTAATATAACTCAGCTGACGTCGTCAATGGAGGAAAGTCTCAAGTCAGTCGAGAAAACAGATTGGCTACTTCGCCCATCAGCTGGTATGAGATCTTGCTGCATCTTCAAAGTACCCCAATGGTTGGTCGAAGTCAACGGCCAGACCTACCACCCGCATATAGTCTCCATCGGTCCATATCACCACGGCAAGCCAGATCTAGAGATGATGCAGCAACACAAATGGAGATATCTTCGCGACTTACTTGCTCGAACACCACCAACTGGCCCCACACTTGCTGATTACTTGCAGCTTGTAGCTTTTAAGGAGGAAGACATACGAGGGTGCTATTCTGAGACCCGCAAGTTGAGCAAAAGCGAGCTCGTTCAGATGATGGTTGTAGACGGTCTCTTCATTATCGAAATCTTCTTCAGAGTTGAACGTTTATCAGAAAGAGATCCATGCGTTGATCCGGACGACGCCATCTTTAACTTGAATTGGTTACTGCCTAAGCTCATGCTAGACCTTCTTCGGCTAGAAAACCAAATTCCTTTCTTCGTTCTTGAAATGTTATTTAATCAATCGAAACCTTCAAGACCGAACTGTGATGAGTACTCATCCCTTAGCAAGGCTTCTTTAAAATTCTTCAATTTTGCAGTGCTACAAAGATCTGATGAAGACTTGGAAAAATATTATCATCTAGGAGGAGTACATCTACTCGATTTAATTCGCAAGACTTATATCCACGATATTCCTCATCCTCCTCGAGGAAATGCTTTCGAGTCAGTTGATAAGATTCGATCAGTGAAAAAGCTTCATCAAGCGGGAATTAAGTTTAAGAAAGGGGAGGCAGCCAGCTTCTTAGATATCAAATTCCGCAACGGAGTGCTAGAAGTTCCGCAGATGACAGTGGACGATCTCCGTGTCCATCTATTCCTGAATTTCGTTGCATTTGAACAATGTTATAACCATTGCCACAAGCACATAACCACTTATGCTGTATTCATGAACTGCCTCAACGGCACGCCTGCGGATACAAGCTTCCTCTGTGACAGGAACATTATCGAGAATTATCTTGGATCCGACGAGGAGGTCACTCATTTCTTCAAAAATCTTGGCAAAGATGCGGCTTTCGACATCAATGCGAGTTACTTGCTGCAATTATTCAAGGATGTGAATGAGCACTGCAATAATGTTTGGAACGTGCAATGGGCAGGTTTTATGTTGACGTATTTTGATACCCCTTGGTCTTTTATGTCTGCCTCAGCTGTTTTAATTGCCTTATTACTTACCGCAATCCAAACTGTTTTAGCCATTTATGCATCTGACGGTCCAGGGGGGCATGGCGGAAGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

478

Amino Acids

54.27

Weight (kDa)

5.94

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 67 - 455 5.6e-113 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
AasI GACNNNNNNGTC 1 cut(s) 1166
AatII GACGTC 1 cut(s) 119
AbsI CCTCGAGG 1 cut(s) 840
Acc36I ACCTGC 1 cut(s) 1280
AccB7I CCANNNNNTGG 3 cut(s) 179, 220, 359
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 333
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 9 cut(s) 251, 436, 899, 952, 974, 1106, 1202, 1368, 1422
AclI AACGTT 1 cut(s) 517
AclWI GGATC 4 cut(s) 527, 539, 1149, 1162
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 4 cut(s) 621, 716, 947, 1015
AcuI CTGAAG 1 cut(s) 490
AcyI GRCGYC 3 cut(s) 93, 116, 555
AfaI GTAC 3 cut(s) 210, 689, 786
AfiI CCNNNNNNNGG 5 cut(s) 179, 220, 359, 703, 842
AjnI CCWGG 1 cut(s) 1408
Alw21I GWGCWC 2 cut(s) 459, 1260
Alw26I GTCTC 5 cut(s) 51, 140, 265, 425, 488
AlwI GGATC 4 cut(s) 527, 539, 1149, 1162
Ama87I CYCGRG 1 cut(s) 840
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 2 cut(s) 235, 361
ApeKI GCWGC 5 cut(s) 195, 307, 385, 926, 1231
ApoI RAATTY 4 cut(s) 621, 716, 947, 1015
AseI ATTAAT 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 269, 362, 1406
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 840
AvaII GGWCC 2 cut(s) 269, 1406
BamHI GGATCC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 459
BbsI GAAGAC 2 cut(s) 414, 759
Bbv12I GWGCWC 2 cut(s) 459, 1260
BbvCI CCTCAGC 1 cut(s) 1339
BbvI GCAGC 5 cut(s) 182, 319, 397, 938, 1218
BccI CCATC 6 cut(s) 19, 181, 272, 463, 566, 1011
BceAI ACGGC 3 cut(s) 250, 298, 1111
BcgI CGANNNNNNTGC 6 cut(s) 323, 357, 1199, 1210, 1233, 1244
BciT130I CCWGG 1 cut(s) 1410
BciVI GTATCC 1 cut(s) 1102
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 5 cut(s) 51, 140, 265, 425, 488
BfaI CTAG 5 cut(s) 296, 596, 611, 776, 965
BfuAI ACCTGC 1 cut(s) 1280
BfuI GTATCC 1 cut(s) 1102
BglI GCCNNNNNGGC 1 cut(s) 1095
BglII AGATCT 3 cut(s) 188, 292, 743
BisI GCNGC 6 cut(s) 196, 308, 386, 927, 1203, 1232
BlsI GCNGC 6 cut(s) 197, 309, 387, 928, 1204, 1233
BmcAI AGTACT 1 cut(s) 689
Bme1390I CCNGG 2 cut(s) 89, 1410
Bme18I GGWCC 2 cut(s) 269, 1406
BmeT110I CYCGRG 1 cut(s) 840
BmgT120I GGNCC 3 cut(s) 269, 362, 1406
BmiI GGNNCC 2 cut(s) 364, 1156
BmrFI CCNGG 2 cut(s) 89, 1410
BmsI GCATC 5 cut(s) 92, 207, 294, 1189, 1406
BpiI GAAGAC 2 cut(s) 414, 759
Bpu10I CCTNAGC 3 cut(s) 585, 698, 1339
BpuEI CTTGAG 2 cut(s) 10, 122
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 3 cut(s) 93, 116, 555
BsaI GGTCTC 2 cut(s) 425, 488
BsaJI CCNNGG 4 cut(s) 87, 280, 1319, 1409
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 220, 359, 703, 842
Bse1I ACTGG 1 cut(s) 364
Bse3DI GCAATG 2 cut(s) 1045, 1289
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 1 cut(s) 1410
BseDI CCNNGG 4 cut(s) 87, 280, 1319, 1409
BseGI GGATG 5 cut(s) 70, 83, 692, 832, 1252
BseLI CCNNNNNNNGG 5 cut(s) 179, 220, 359, 703, 842
BseMI GCAATG 2 cut(s) 1045, 1289
BseMII CTCAG 3 cut(s) 122, 420, 1353
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 3 cut(s) 795, 828, 1178
BseXI GCAGC 5 cut(s) 182, 319, 397, 938, 1218
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 237, 363
BsiHKAI GWGCWC 2 cut(s) 459, 1260
BsiHKCI CYCGRG 1 cut(s) 840
BsiSI CCGG 2 cut(s) 89, 548
BslI CCNNNNNNNGG 5 cut(s) 179, 220, 359, 703, 842
BsmAI GTCTC 5 cut(s) 51, 140, 265, 425, 488
BsmBI CGTCTC 1 cut(s) 51
BsnI GGCC 2 cut(s) 237, 363
Bso31I GGTCTC 2 cut(s) 425, 488
BsoBI CYCGRG 1 cut(s) 840
Bsp1286I GDGCHC 2 cut(s) 459, 1260
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
Bsp68I TCGCGA 1 cut(s) 333
BspACI CCGC 9 cut(s) 251, 436, 899, 952, 974, 1106, 1202, 1368, 1422
BspANI GGCC 2 cut(s) 237, 363
BspCNI CTCAG 3 cut(s) 121, 421, 1352
BspEI TCCGGA 1 cut(s) 547
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 2 cut(s) 364, 1156
BspMI ACCTGC 1 cut(s) 1280
BspPI GGATC 4 cut(s) 527, 539, 1149, 1162
BspTNI GGTCTC 2 cut(s) 425, 488
BsrDI GCAATG 2 cut(s) 1045, 1289
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 4 cut(s) 87, 280, 1319, 1409
BssMI GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
BssNI GRCGYC 3 cut(s) 93, 116, 555
BssT1I CCWWGG 1 cut(s) 1319
Bst2UI CCWGG 1 cut(s) 1410
Bst4CI ACNGT 5 cut(s) 482, 680, 985, 1381, 1406
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 3 cut(s) 93, 116, 555
BstC8I GCNNGC 5 cut(s) 287, 455, 931, 1100, 1104
BstDEI CTNAG 6 cut(s) 108, 429, 585, 698, 937, 1339
BstDSI CCRYGG 1 cut(s) 280
BstENI CCTNNNNNAGG 2 cut(s) 701, 840
BstF5I GGATG 5 cut(s) 70, 83, 692, 832, 1252
BstFNI CGCG 1 cut(s) 333
BstKTI GATC 8 cut(s) 191, 295, 535, 547, 746, 878, 994, 1157
BstMAI GTCTC 5 cut(s) 51, 140, 265, 425, 488
BstMBI GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
BstMWI GCNNNNNNNGC 3 cut(s) 444, 1095, 1394
BstNI CCWGG 1 cut(s) 1410
BstSCI CCNGG 2 cut(s) 87, 1408
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 5 cut(s) 182, 319, 397, 938, 1218
BstV2I GAAGAC 2 cut(s) 414, 759
BstX2I RGATCY 5 cut(s) 188, 292, 532, 743, 1154
BstYI RGATCY 5 cut(s) 188, 292, 532, 743, 1154
BsuI GTATCC 1 cut(s) 1102
BsuRI GGCC 2 cut(s) 237, 363
BtgI CCRYGG 1 cut(s) 280
BtsCI GGATG 5 cut(s) 70, 83, 692, 832, 1252
BtsI GCAGTG 2 cut(s) 738, 1258
BtsIMutI CAGTG 4 cut(s) 738, 885, 990, 1258
BtuMI TCGCGA 1 cut(s) 333
BveI ACCTGC 1 cut(s) 1280
Cac8I GCNNGC 5 cut(s) 287, 455, 931, 1100, 1104
CciI TCATGA 1 cut(s) 1080
Cfr13I GGNCC 3 cut(s) 269, 362, 1406
CseI GACGC 2 cut(s) 101, 563
Csp6I GTAC 3 cut(s) 209, 688, 785
CviAII CATG 4 cut(s) 537, 592, 1081, 1418
CviQI GTAC 3 cut(s) 209, 688, 785
DdeI CTNAG 6 cut(s) 108, 429, 585, 698, 937, 1339
DpnI GATC 8 cut(s) 190, 294, 534, 546, 745, 877, 993, 1156
DpnII GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
DraI TTTAAA 1 cut(s) 714
DrdI GACNNNNNNGTC 1 cut(s) 1166
DseDI GACNNNNNNGTC 1 cut(s) 1166
EaeI YGGCCR 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 1319
Eco24I GRGCYC 1 cut(s) 459
Eco31I GGTCTC 2 cut(s) 425, 488
Eco32I GATATC 2 cut(s) 326, 943
Eco47I GGWCC 2 cut(s) 269, 1406
Eco53kI GAGCTC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 840
EcoICRI GAGCTC 1 cut(s) 457
EcoNI CCTNNNNNAGG 2 cut(s) 701, 840
EcoRII CCWGG 1 cut(s) 1408
EcoRV GATATC 2 cut(s) 326, 943
EcoT14I CCWWGG 1 cut(s) 1319
EcoT22I ATGCAT 1 cut(s) 1399
EcoT38I GRGCYC 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 1319
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 4 cut(s) 540, 595, 1084, 1421
FatI CATG 4 cut(s) 536, 591, 1080, 1417
FauI CCCGC 3 cut(s) 258, 443, 892
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 6 cut(s) 196, 308, 386, 927, 1203, 1232
FokI GGATG 5 cut(s) 70, 77, 679, 819, 1259
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 6 cut(s) 196, 308, 386, 927, 1203, 1232
FspBI CTAG 5 cut(s) 296, 596, 611, 776, 965
GluI GCNGC 6 cut(s) 196, 308, 386, 927, 1203, 1232
HaeIII GGCC 2 cut(s) 237, 363
HapII CCGG 2 cut(s) 89, 548
HgaI GACGC 2 cut(s) 101, 563
Hin1I GRCGYC 3 cut(s) 93, 116, 555
Hin1II CATG 4 cut(s) 540, 595, 1084, 1421
HincII GTYRAC 2 cut(s) 232, 1302
HindII GTYRAC 2 cut(s) 232, 1302
HindIII AAGCTT 2 cut(s) 887, 1114
HinfI GANTC 2 cut(s) 857, 871
HpaII CCGG 2 cut(s) 89, 548
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 4 cut(s) 232, 478, 988, 1302
Hpy188I TCNGA 7 cut(s) 430, 465, 509, 526, 748, 1159, 1402
Hpy188III TCNNGA 9 cut(s) 148, 296, 332, 548, 638, 669, 1012, 1081, 1141
Hpy8I GTNNAC 4 cut(s) 232, 478, 988, 1302
Hpy99I CGWCG 3 cut(s) 121, 557, 1163
HpyAV CCTTC 3 cut(s) 611, 675, 1419
HpyCH4III ACNGT 5 cut(s) 482, 680, 985, 1381, 1406
HpyCH4IV ACGT 4 cut(s) 116, 517, 1278, 1304
HpyCH4V TGCA 9 cut(s) 198, 307, 385, 731, 1025, 1234, 1263, 1282, 1397
HpyF10VI GCNNNNNNNGC 3 cut(s) 444, 1095, 1394
HpyF3I CTNAG 6 cut(s) 108, 429, 585, 698, 937, 1339
HpySE526I ACGT 4 cut(s) 116, 517, 1278, 1304
Hsp92I GRCGYC 3 cut(s) 93, 116, 555
Hsp92II CATG 4 cut(s) 540, 595, 1084, 1421
Kpn2I TCCGGA 1 cut(s) 547
Kzo9I GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
Lsp1109I GCAGC 5 cut(s) 182, 319, 397, 938, 1218
LweI GCATC 5 cut(s) 92, 207, 294, 1189, 1406
MaeI CTAG 5 cut(s) 296, 596, 611, 776, 965
MaeII ACGT 4 cut(s) 116, 517, 1278, 1304
MaeIII GTNAC 4 cut(s) 576, 1124, 1168, 1223
MalI GATC 8 cut(s) 190, 294, 534, 546, 745, 877, 993, 1156
MboI GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
MflI RGATCY 5 cut(s) 188, 292, 532, 743, 1154
MhlI GDGCHC 2 cut(s) 459, 1260
MlyI GAGTC 1 cut(s) 866
MmeI TCCRAC 1 cut(s) 1182
Mph1103I ATGCAT 1 cut(s) 1399
MroI TCCGGA 1 cut(s) 547
MseI TTAA 9 cut(s) 99, 399, 564, 651, 713, 800, 906, 912, 1349
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 3 cut(s) 112, 179, 1343
MspI CCGG 2 cut(s) 89, 548
MspR9I CCNGG 2 cut(s) 89, 1410
MvaI CCWGG 1 cut(s) 1410
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 3 cut(s) 444, 1095, 1394
NciI CCSGG 1 cut(s) 89
NdeII GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
NlaIII CATG 4 cut(s) 540, 595, 1084, 1421
NlaIV GGNNCC 2 cut(s) 364, 1156
NmuCI GTSAC 2 cut(s) 1124, 1168
NruI TCGCGA 1 cut(s) 333
NsiI ATGCAT 1 cut(s) 1399
PaeR7I CTCGAG 1 cut(s) 840
PagI TCATGA 1 cut(s) 1080
PfeI GAWTC 1 cut(s) 871
PflFI GACNNNGTC 1 cut(s) 118
PflMI CCANNNNNTGG 3 cut(s) 179, 220, 359
PkrI GCNGC 6 cut(s) 197, 309, 387, 928, 1204, 1233
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
PshBI ATTAAT 1 cut(s) 99
PsiI TTATAA 1 cut(s) 1041
Psp124BI GAGCTC 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 1408
PspGI CCWGG 1 cut(s) 1408
PspN4I GGNNCC 2 cut(s) 364, 1156
PspPI GGNCC 3 cut(s) 269, 362, 1406
PspXI VCTCGAGB 1 cut(s) 840
PsuI RGATCY 5 cut(s) 188, 292, 532, 743, 1154
PsyI GACNNNGTC 1 cut(s) 118
PvuII CAGCTG 3 cut(s) 112, 179, 1343
RruI TCGCGA 1 cut(s) 333
RsaI GTAC 3 cut(s) 210, 689, 786
RsaNI GTAC 3 cut(s) 209, 688, 785
RseI CAYNNNNRTG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 459
SaqAI TTAA 9 cut(s) 99, 399, 564, 651, 713, 800, 906, 912, 1349
SatI GCNGC 6 cut(s) 196, 308, 386, 927, 1203, 1232
Sau3AI GATC 8 cut(s) 188, 292, 532, 544, 743, 875, 991, 1154
Sau96I GGNCC 3 cut(s) 269, 362, 1406
ScaI AGTACT 1 cut(s) 689
SchI GAGTC 1 cut(s) 866
ScrFI CCNGG 2 cut(s) 89, 1410
SduI GDGCHC 2 cut(s) 459, 1260
SfaNI GCATC 5 cut(s) 92, 207, 294, 1189, 1406
Sfr274I CTCGAG 1 cut(s) 840
SinI GGWCC 2 cut(s) 269, 1406
SlaI CTCGAG 1 cut(s) 840
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 3 cut(s) 25, 137, 840
SmoI CTYRAG 3 cut(s) 25, 137, 840
SsiI CCGC 9 cut(s) 251, 436, 899, 952, 974, 1106, 1202, 1368, 1422
SspI AATATT 1 cut(s) 767
SspMI CTAG 5 cut(s) 296, 596, 611, 776, 965
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 87, 1408
StyI CCWWGG 1 cut(s) 1319
TaaI ACNGT 5 cut(s) 482, 680, 985, 1381, 1406
TaiI ACGT 4 cut(s) 119, 520, 1281, 1307
TaqII GACCGA 2 cut(s) 257, 688
TatI WGTACW 2 cut(s) 687, 784
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 1 cut(s) 871
Tru1I TTAA 9 cut(s) 99, 399, 564, 651, 713, 800, 906, 912, 1349
Tru9I TTAA 9 cut(s) 99, 399, 564, 651, 713, 800, 906, 912, 1349
TscAI CASTG 4 cut(s) 738, 885, 990, 1265
TseFI GTSAC 2 cut(s) 1124, 1168
TseI GCWGC 5 cut(s) 195, 307, 385, 926, 1231
Tsp45I GTSAC 2 cut(s) 1124, 1168
TspDTI ATGAA 5 cut(s) 478, 765, 881, 1069, 1097
TspGWI ACGGA 2 cut(s) 972, 986
TspRI CASTG 4 cut(s) 738, 885, 990, 1265
Tth111I GACNNNGTC 1 cut(s) 118
Van91I CCANNNNNTGG 3 cut(s) 179, 220, 359
VpaK11BI GGWCC 2 cut(s) 269, 1406
VspI ATTAAT 1 cut(s) 99
XagI CCTNNNNNAGG 2 cut(s) 701, 840
XapI RAATTY 4 cut(s) 621, 716, 947, 1015
XbaI TCTAGA 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 840
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 5 cut(s) 296, 596, 611, 776, 965
ZraI GACGTC 1 cut(s) 117
ZrmI AGTACT 1 cut(s) 689
Zsp2I ATGCAT 1 cut(s) 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.