pycom07g10000

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002062
Physical Location & Seq
Forward (+)
9614510 .. 9614803
294 bp
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UTR
Exon/CDS
Intron
pycom07g10000.1

Sequence Viewer

Length: 294 bp
ATGGCAAATACAGGAAGAGATCATACTGCCGTTACAGTTACCACTACGGAATATAATCGCACGACGTCGTTGAAGCAAAGAATCGAAGGAACGAAATGGCTACTGCATCCGTTAGCAGGTAAGAGCTCTTGTTGCATCTTCAAAGTACCTCATTGCCTAGCGAAAACGAACAAGGGCAAGTACCGGCCTCATATCATCTCAATCGGCCCTTATCACTACGGTGATAAACACGTGGTGATGAAGCAACAACACAAATGGAGATTTCTTGACAATCTTCTCGGTCGAACGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

98

Amino Acids

11.19

Weight (kDa)

10.14

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 46 - 95 2.6e-13 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 107
AccII CGCG 1 cut(s) 289
AciI CCGC 1 cut(s) 289
AcvI CACGTG 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 2 cut(s) 147, 182
AfiI CCNNNNNNNGG 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 2 cut(s) 73, 142
AleI CACNNNNGTG 1 cut(s) 219
AluBI AGCT 1 cut(s) 126
AluI AGCT 1 cut(s) 126
Alw21I GWGCWC 1 cut(s) 128
AoxI GGCC 2 cut(s) 185, 205
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 233, 247
BanII GRGCYC 1 cut(s) 128
BarI GAAGNNNNNNTAC 2 cut(s) 7, 39
BbrPI CACGTG 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 14
BfaI CTAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 206
BmsI GCATC 2 cut(s) 115, 144
BsaAI YACGTR 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse118I RCCGGY 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 151
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 187, 207
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 116
BsnI GGCC 2 cut(s) 187, 207
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 19
BspACI CCGC 1 cut(s) 289
BspANI GGCC 2 cut(s) 187, 207
BspFNI CGCG 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 183
BssAI RCCGGY 1 cut(s) 183
BssMI GATC 1 cut(s) 19
BssNI GRCGYC 1 cut(s) 65
Bst4CI ACNGT 2 cut(s) 37, 221
Bst6I CTCTTC 1 cut(s) 10
BstACI GRCGYC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 232
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 289
BstKTI GATC 1 cut(s) 22
BstMBI GATC 1 cut(s) 19
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstUI CGCG 1 cut(s) 289
BsuRI GGCC 2 cut(s) 187, 207
BtsCI GGATG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 146, 181
CviJI RGCY 4 cut(s) 100, 126, 187, 207
CviKI_1 RGCY 4 cut(s) 100, 126, 187, 207
CviQI GTAC 2 cut(s) 146, 181
DpnI GATC 1 cut(s) 21
DpnII GATC 1 cut(s) 19
DraIII CACNNNGTG 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 126
Eco72I CACGTG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 128
FaiI YATR 3 cut(s) 24, 54, 192
FokI GGATG 1 cut(s) 93
FriOI GRGCYC 1 cut(s) 128
FspBI CTAG 1 cut(s) 158
HaeIII GGCC 2 cut(s) 187, 207
HapII CCGG 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 65
HinfI GANTC 1 cut(s) 81
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 233, 247
Hpy188III TCNNGA 1 cut(s) 266
Hpy99I CGWCG 2 cut(s) 67, 70
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 37, 221
HpyCH4IV ACGT 2 cut(s) 65, 231
HpyCH4V TGCA 2 cut(s) 106, 135
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpySE526I ACGT 2 cut(s) 65, 231
Hsp92I GRCGYC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 19
LpnPI CCDG 2 cut(s) 102, 197
LweI GCATC 2 cut(s) 115, 144
MaeI CTAG 1 cut(s) 158
MaeII ACGT 2 cut(s) 65, 231
MaeIII GTNAC 2 cut(s) 31, 37
MalI GATC 1 cut(s) 21
MboI GATC 1 cut(s) 19
MboII GAAGA 3 cut(s) 27, 130, 266
MhlI GDGCHC 1 cut(s) 128
MnlI CCTC 2 cut(s) 159, 198
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 1 cut(s) 19
OliI CACNNNNGTG 1 cut(s) 219
PfeI GAWTC 1 cut(s) 81
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
Ppu21I YACGTR 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 128
PspCI CACGTG 1 cut(s) 232
PspPI GGNCC 1 cut(s) 206
RsaI GTAC 2 cut(s) 147, 182
RsaNI GTAC 2 cut(s) 146, 181
RseI CAYNNNNRTG 1 cut(s) 219
SacI GAGCTC 1 cut(s) 128
Sau3AI GATC 1 cut(s) 19
Sau96I GGNCC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 128
SetI ASST 5 cut(s) 68, 121, 128, 151, 234
SfaNI GCATC 2 cut(s) 115, 144
SmiMI CAYNNNNRTG 1 cut(s) 219
SsiI CCGC 1 cut(s) 289
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 128
TaaI ACNGT 2 cut(s) 37, 221
TaiI ACGT 2 cut(s) 68, 234
TaqI TCGA 2 cut(s) 84, 283
TaqII GACCGA 1 cut(s) 269
TfiI GAWTC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 2 cut(s) 62, 99
XspI CTAG 1 cut(s) 158
ZraI GACGTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.