AT3G54430

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002187
Physical Location & Seq
Reverse (-)
20146626 .. 20148221
1596 bp
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UTR
Exon/CDS
Intron
AT3G54430.1

Sequence Viewer

Length: 552 bp
ATGTTAGGTCTTCGAAACATCATTCTCTTATCTCCACCACCGACGCAGATAACACGGCCATCTCTTCCTCCGGTAAACTTCGCGGCGGTGGAAGACAACAACACAGTTGGAGAGAAAGTATGCAGAGACTGTGGAAACAGAGCAAAGAAAGAGTGTTTGTTCGAAAGATGTAGAACTTGTTGTAAAAGCAGAGGATACAACTGTGTCACTCACGTGAAGAGCACGTGGATTCCTTCTTCTGCAACTCGTTCTTCATCTTCTCCTTCTGAGAGGAAGAAGAAGCTCAAAATCGATAAACAGAGTTCCCCTAATGTCTCGCTACTTCCGACAACCACTTCTCGTCAAGAGAGAGGCTTCAGAGAGGGGTTACCGGGGAAAATTGAAGCTCCGGCGGTTTTTAAACGGACGAGAGTAACAGCGATAAGCAACAACGAGCAAGCAGAGATTGGTTATCAAGCAACAGTAACTATAAGTGGTCATATCTTCAAAGGCTTTCTTCATTACTATGGTGTTGATCATAACAAAGCTTTTCCATGTCTTTCTCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

183

Amino Acids

20.33

Weight (kDa)

9.77

Isoelectric Point (pI)

59.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 38 - 172 7.6e-41 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 83, 86, 392
AcoI YGGCCR 1 cut(s) 56
AcuI CTGAAG 1 cut(s) 340
AcvI CACGTG 2 cut(s) 214, 225
AgsI TTSAA 2 cut(s) 383, 487
AleI CACNNNNGTG 1 cut(s) 212
AluBI AGCT 3 cut(s) 283, 386, 527
AluI AGCT 3 cut(s) 283, 386, 527
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 120, 319
AlwNI CAGNNNCTG 1 cut(s) 129
AoxI GGCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 372
AsuII TTCGAA 2 cut(s) 13, 162
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BbrPI CACGTG 2 cut(s) 214, 225
BbsI GAAGAC 2 cut(s) 2, 99
Bbv12I GWGCWC 1 cut(s) 224
BccI CCATC 1 cut(s) 67
BceAI ACGGC 1 cut(s) 71
BciVI GTATCC 1 cut(s) 188
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 120, 319
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 372
BmrFI CCNGG 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 2, 99
Bpu14I TTCGAA 2 cut(s) 13, 162
BpuMI CCSGG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 291
BsaAI YACGTR 2 cut(s) 214, 225
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 371
BseMII CTCAG 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 1 cut(s) 58
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 3 cut(s) 71, 371, 389
BsmAI GTCTC 2 cut(s) 120, 319
BsnI GGCC 1 cut(s) 58
Bsp119I TTCGAA 2 cut(s) 13, 162
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 1 cut(s) 514
BspACI CCGC 3 cut(s) 83, 86, 392
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 212
BspT104I TTCGAA 2 cut(s) 13, 162
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 1 cut(s) 514
Bst4CI ACNGT 4 cut(s) 106, 131, 203, 463
Bst6I CTCTTC 2 cut(s) 69, 212
BstBAI YACGTR 2 cut(s) 214, 225
BstBI TTCGAA 2 cut(s) 13, 162
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 366
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 1 cut(s) 517
BstMAI GTCTC 2 cut(s) 120, 319
BstMBI GATC 1 cut(s) 514
BstPI GGTNACC 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 1 cut(s) 83
BstV2I GAAGAC 2 cut(s) 2, 99
Bsu15I ATCGAT 1 cut(s) 291
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 1 cut(s) 58
BsuTUI ATCGAT 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 438
CaiI CAGNNNCTG 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 1 cut(s) 52
CviAII CATG 1 cut(s) 534
CviJI RGCY 6 cut(s) 58, 283, 354, 386, 492, 527
CviKI_1 RGCY 6 cut(s) 58, 283, 354, 386, 492, 527
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 516
DpnII GATC 1 cut(s) 514
DraI TTTAAA 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 56
Eam1104I CTCTTC 2 cut(s) 69, 212
EarI CTCTTC 2 cut(s) 69, 212
Eco57I CTGAAG 1 cut(s) 340
Eco72I CACGTG 2 cut(s) 214, 225
Eco91I GGTNACC 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 366
FaeI CATG 1 cut(s) 537
FaiI YATR 6 cut(s) 121, 470, 480, 507, 519, 535
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FatI CATG 1 cut(s) 533
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 58
HapII CCGG 3 cut(s) 71, 371, 389
HgaI GACGC 1 cut(s) 52
Hin1II CATG 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 1 cut(s) 229
HpaII CCGG 3 cut(s) 71, 371, 389
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 3 cut(s) 268, 327, 359
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 243, 273
HpyCH4III ACNGT 4 cut(s) 106, 131, 203, 463
HpyCH4IV ACGT 2 cut(s) 213, 224
HpyCH4V TGCA 2 cut(s) 123, 242
HpyF3I CTNAG 1 cut(s) 267
HpySE526I ACGT 2 cut(s) 213, 224
Hsp92II CATG 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 1 cut(s) 514
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 3 cut(s) 84, 384, 402
MaeII ACGT 2 cut(s) 213, 224
MaeIII GTNAC 4 cut(s) 205, 366, 412, 463
MalI GATC 1 cut(s) 516
MboI GATC 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 1 cut(s) 378
MmeI TCCRAC 2 cut(s) 88, 350
MnlI CCTC 5 cut(s) 78, 185, 264, 344, 355
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 2 cut(s) 212, 504
MspI CCGG 3 cut(s) 71, 371, 389
MspR9I CCNGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 83
NciI CCSGG 1 cut(s) 372
NdeII GATC 1 cut(s) 514
NlaIII CATG 1 cut(s) 537
NmuCI GTSAC 1 cut(s) 205
NspV TTCGAA 2 cut(s) 13, 162
OliI CACNNNNGTG 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 212
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PfeI GAWTC 1 cut(s) 229
PkrI GCNGC 1 cut(s) 85
PmaCI CACGTG 2 cut(s) 214, 225
PmlI CACGTG 2 cut(s) 214, 225
Ppu21I YACGTR 2 cut(s) 214, 225
PspCI CACGTG 2 cut(s) 214, 225
PspEI GGTNACC 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 129
RseI CAYNNNNRTG 2 cut(s) 212, 504
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 514
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 224
SetI ASST 6 cut(s) 10, 216, 227, 285, 388, 529
SfuI TTCGAA 2 cut(s) 13, 162
SmiMI CAYNNNNRTG 2 cut(s) 212, 504
Sse9I AATT 1 cut(s) 378
SsiI CCGC 3 cut(s) 83, 86, 392
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 4 cut(s) 106, 131, 203, 463
TaiI ACGT 2 cut(s) 216, 227
TaqI TCGA 3 cut(s) 13, 162, 291
TasI AATT 1 cut(s) 378
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 243, 488
TspGWI ACGGA 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.