Rroxscaffold_4G00325640

Protein LATERAL ROOT PRIMORDIUM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002187
Physical Location & Seq
Reverse (-)
57411686 .. 57415638
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325640.1

Sequence Viewer

Length: 495 bp
ATGTCCATACTATGTCGAGTTTCAGTTGTCGTCGTAGATTTCGGCGAGCGATTGGCTTTCGGTAGCTGGTTCATTGCTAATAGTACCCAGTCTTACTGCGGCTTCATGCCGTGGTTGCATGCTGGCTTCATGGCGTGTGTTTCGTTTGGTCAACTTAGCACAATCAGCTCCTCAGGTCGGTCTTCGTCGTTGGATCTATTGACGGATGCAAATCTCAGACAGTCATTACCAGATCAAGTTCGGGTACCAGCTGTTTTTAAGTGCCATAGAGTCACTGCCATTAGCAATGGTGAAGCTGAGATTGCTTACCAGGCTACTGTGAAAATCAGTGGTCATGTATTCAAGGGGTTGCTCTATGATCGTGGGGTTGATAAGAAGAATGAATACCCTTGTATTTCACATATGCATTTGGGAAGTGGTAGTAACCAAAGGAATGGAGACTCTTCTTCTCCAACTGTGCCTCCATCCAATGTCTGTGTGGCGTCCACCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

164

Amino Acids

17.67

Weight (kDa)

8.26

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 62 - 124 8.9e-22 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 244
AciI CCGC 1 cut(s) 99
AclWI GGATC 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 2 cut(s) 85, 246
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 1 cut(s) 343
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 4 cut(s) 66, 168, 251, 296
AluI AGCT 4 cut(s) 66, 168, 251, 296
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 172
BanI GGYRCC 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 1 cut(s) 174
BccI CCATC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 432
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 311
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 88
Bse21I CCTNAGG 1 cut(s) 172
Bse3DI GCAATG 2 cut(s) 72, 292
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 2 cut(s) 211, 464
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 2 cut(s) 72, 292
BseMII CTCAG 3 cut(s) 186, 229, 288
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 1 cut(s) 432
Bsp143I GATC 3 cut(s) 193, 232, 358
BspACI CCGC 1 cut(s) 99
BspCNI CTCAG 3 cut(s) 185, 228, 289
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 72, 292
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 3 cut(s) 193, 232, 358
BssNI GRCGYC 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 222, 319, 457
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 47, 120, 124
BstDEI CTNAG 4 cut(s) 155, 172, 215, 297
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 211, 464
BstKTI GATC 3 cut(s) 196, 235, 361
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 3 cut(s) 193, 232, 358
BstMWI GCNNNNNNNGC 4 cut(s) 115, 165, 302, 311
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 309
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 172
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 2 cut(s) 211, 464
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 273, 334
Cac8I GCNNGC 3 cut(s) 47, 120, 124
CseI GACGC 1 cut(s) 471
Csp6I GTAC 2 cut(s) 84, 245
CviAII CATG 4 cut(s) 106, 119, 130, 335
CviJI RGCY 8 cut(s) 56, 66, 102, 126, 168, 251, 296, 314
CviKI_1 RGCY 8 cut(s) 56, 66, 102, 126, 168, 251, 296, 314
CviQI GTAC 2 cut(s) 84, 245
DdeI CTNAG 4 cut(s) 155, 172, 215, 297
DpnI GATC 3 cut(s) 195, 234, 360
DpnII GATC 3 cut(s) 193, 232, 358
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
Eco81I CCTNAGG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 408
FaeI CATG 4 cut(s) 109, 122, 133, 338
FatI CATG 4 cut(s) 105, 118, 129, 334
FauNDI CATATG 1 cut(s) 402
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 2 cut(s) 218, 451
Fsp4HI GCNGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 100
HgaI GACGC 1 cut(s) 471
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 4 cut(s) 109, 122, 133, 338
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HinfI GANTC 2 cut(s) 270, 440
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 2 cut(s) 152, 486
Hpy188I TCNGA 1 cut(s) 218
Hpy8I GTNNAC 2 cut(s) 152, 486
Hpy99I CGWCG 2 cut(s) 35, 190
HpyCH4III ACNGT 3 cut(s) 222, 319, 457
HpyCH4V TGCA 3 cut(s) 118, 209, 406
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 165, 302, 311
HpyF3I CTNAG 4 cut(s) 155, 172, 215, 297
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 4 cut(s) 109, 122, 133, 338
KpnI GGTACC 1 cut(s) 248
Kzo9I GATC 3 cut(s) 193, 232, 358
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 8 cut(s) 52, 101, 108, 159, 243, 261, 296, 323
LweI GCATC 1 cut(s) 196
MaeIII GTNAC 2 cut(s) 271, 422
MalI GATC 3 cut(s) 195, 234, 360
MboI GATC 3 cut(s) 193, 232, 358
MboII GAAGA 4 cut(s) 174, 388, 435, 438
MflI RGATCY 1 cut(s) 193
MlyI GAGTC 2 cut(s) 279, 434
MmeI TCCRAC 2 cut(s) 171, 476
MnlI CCTC 2 cut(s) 181, 471
Mph1103I ATGCAT 1 cut(s) 408
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 115, 165, 302, 311
NdeI CATATG 1 cut(s) 402
NdeII GATC 3 cut(s) 193, 232, 358
NlaIII CATG 4 cut(s) 109, 122, 133, 338
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 2 cut(s) 278, 434
PpsI GAGTC 2 cut(s) 278, 434
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 246
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 85, 246
RsaNI GTAC 2 cut(s) 84, 245
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 3 cut(s) 193, 232, 358
SchI GAGTC 2 cut(s) 279, 434
ScrFI CCNGG 1 cut(s) 311
SetI ASST 6 cut(s) 68, 170, 178, 253, 298, 494
SfaNI GCATC 1 cut(s) 196
SphI GCATGC 1 cut(s) 122
SsiI CCGC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 222, 319, 457
TaqI TCGA 1 cut(s) 16
TaqII GACCGA 1 cut(s) 168
TauI GCSGC 1 cut(s) 102
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 2 cut(s) 280, 334
TseFI GTSAC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 4 cut(s) 61, 94, 118, 396
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 280, 334
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.