RLG00000017375

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002187
Physical Location & Seq
Reverse (-)
15838915 .. 15842038
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017375

Sequence Viewer

Length: 786 bp
ATGTTGAAGCACGACTCAGAGCCATTGGATGCCACTCGGTTACTAGTGCTCTCATTGGGCACCGGCTCCGCCAAGTCTGAAGGGAAGTACACCGACATGTTTGGCGATGCGAGTGCTGATATGGTTGACATTCATGTCTTGACTCTGTTCCAGTCCCTCAATTGCAACCACAACTACATACGCATTCAGACATATGATCTAGACAGATTTGTTCCGTCGTTAGTCAAGATTGTGGTTCCGCTAAAAAAAATGGTCTCTCTCTCGAAGCTCTCTCCTAGGGTTTCCCGGCGGCGGCAGCAGTTTGGTTCTTTTGACTCGGACGAGTGGTCTCCCTCCTCTCTGGTCTCTGATGAAGGTGATCTCAAGGCTGTTGTGCGTTGGATCCTCTTGGCAATAGGAGGCCTCGGCCTCCTTTTGCTTGTTTCTGGCATTCGGTGCCCCTTTGAGTCTCCGGCGATTGGTTATGATGGCTTTACTCCGGTATGGAGTGTTGTGGATCCAAATCTAGCAGGTTGCTCTGGTGGTTGGGCTGAGGGGGAATCGAGCTTCCCTCATGTGTCCGTCGGCGTCAGATTTGGAGCCATATTTGATGTGTGCCGGTGGCGTGTGCAGTGGCCAGGCAATGATGGCAGTGTAATCGGACGGGATGCGGGGCTTTCGGCGTATGGCTCCTCTACTTCATGGGGGTCCTTGGAGTGGAGCGACGTTGGGGCTGTCGGCACACTAGGTGGTCTTCGGGAGGTTTGGGCACTGACGTCAACATGGTGGATCAAAGGCTGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

262

Amino Acids

28.44

Weight (kDa)

5.57

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 1 cut(s) 758
Acc36I ACCTGC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 59, 435
AciI CCGC 5 cut(s) 69, 239, 289, 292, 650
AclWI GGATC 5 cut(s) 376, 389, 491, 504, 776
AcoI YGGCCR 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 2 cut(s) 567, 755
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 3 cut(s) 291, 458, 696
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 7
AhdI GACNNNNNGTC 1 cut(s) 325
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 1 cut(s) 616
AluBI AGCT 2 cut(s) 268, 546
AluI AGCT 2 cut(s) 268, 546
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 4 cut(s) 259, 333, 349, 453
AlwI GGATC 5 cut(s) 376, 389, 491, 504, 776
AoxI GGCC 3 cut(s) 400, 406, 614
ApeKI GCWGC 1 cut(s) 295
AspA2I CCTAGG 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 687
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 368
AvaII GGWCC 1 cut(s) 687
AvrII CCTAGG 1 cut(s) 275
BaeGI GKGCMC 3 cut(s) 62, 440, 751
BalI TGGCCA 1 cut(s) 616
BamHI GGATCC 2 cut(s) 381, 496
BanI GGYRCC 2 cut(s) 59, 435
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 307
BccI CCATC 2 cut(s) 461, 620
BcgI CGANNNNNNTGC 4 cut(s) 95, 129, 619, 653
BciT130I CCWGG 1 cut(s) 618
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 4 cut(s) 259, 333, 349, 453
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 6 cut(s) 44, 200, 276, 506, 725, 784
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 3 cut(s) 290, 293, 296
BlnI CCTAGG 1 cut(s) 275
BlsI GCNGC 3 cut(s) 291, 294, 297
Bme1390I CCNGG 2 cut(s) 286, 618
Bme18I GGWCC 1 cut(s) 687
BmeRI GACNNNNNGTC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 687
BmiI GGNNCC 9 cut(s) 61, 67, 237, 383, 437, 498, 580, 670, 688
BmrFI CCNGG 2 cut(s) 286, 618
BmsI GCATC 3 cut(s) 19, 97, 637
BpiI GAAGAC 1 cut(s) 725
BplI GAGNNNNNCTC 2 cut(s) 535, 567
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 286
BsaBI GATNNNNATC 1 cut(s) 501
BsaHI GRCGYC 2 cut(s) 567, 755
BsaI GGTCTC 3 cut(s) 259, 333, 349
BsaJI CCNNGG 3 cut(s) 275, 403, 690
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 458, 696
Bse118I RCCGGY 2 cut(s) 62, 597
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 3 cut(s) 275, 403, 690
BseGI GGATG 2 cut(s) 34, 652
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 291, 458, 696
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 2 cut(s) 30, 522
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 2 cut(s) 325, 661
BseSI GKGCMC 3 cut(s) 62, 440, 751
BseXI GCAGC 1 cut(s) 307
BsgI GTGCAG 1 cut(s) 629
BshFI GGCC 3 cut(s) 402, 408, 616
BshNI GGYRCC 2 cut(s) 59, 435
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 5 cut(s) 63, 286, 452, 479, 598
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 3 cut(s) 291, 458, 696
BsmAI GTCTC 4 cut(s) 259, 333, 349, 453
BsmFI GGGAC 1 cut(s) 139
BsmI GAATGC 2 cut(s) 183, 429
BsnI GGCC 3 cut(s) 402, 408, 616
Bso31I GGTCTC 3 cut(s) 259, 333, 349
Bsp1286I GDGCHC 4 cut(s) 51, 62, 440, 751
Bsp143I GATC 6 cut(s) 196, 358, 381, 496, 768, 780
BspACI CCGC 5 cut(s) 69, 239, 289, 292, 650
BspANI GGCC 3 cut(s) 402, 408, 616
BspCNI CTCAG 2 cut(s) 29, 523
BspLI GGNNCC 9 cut(s) 61, 67, 237, 383, 437, 498, 580, 670, 688
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 5 cut(s) 376, 389, 491, 504, 776
BspT107I GGYRCC 2 cut(s) 59, 435
BspTNI GGTCTC 3 cut(s) 259, 333, 349
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 2 cut(s) 62, 597
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 2 cut(s) 62, 597
BssECI CCNNGG 3 cut(s) 275, 403, 690
BssMI GATC 6 cut(s) 196, 358, 381, 496, 768, 780
BssNI GRCGYC 2 cut(s) 567, 755
BssT1I CCWWGG 2 cut(s) 275, 690
Bst2UI CCWGG 1 cut(s) 618
BstACI GRCGYC 2 cut(s) 567, 755
BstAPI GCANNNNNTGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 16, 531
BstF5I GGATG 2 cut(s) 34, 652
BstKTI GATC 6 cut(s) 199, 361, 384, 499, 771, 783
BstMAI GTCTC 4 cut(s) 259, 333, 349, 453
BstMBI GATC 6 cut(s) 196, 358, 381, 496, 768, 780
BstMWI GCNNNNNNNGC 3 cut(s) 295, 435, 627
BstNI CCWGG 1 cut(s) 618
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 284, 616
BstSLI GKGCMC 3 cut(s) 62, 440, 751
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 725
BstX2I RGATCY 3 cut(s) 381, 496, 780
BstYI RGATCY 3 cut(s) 381, 496, 780
BsuRI GGCC 3 cut(s) 402, 408, 616
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 34, 652
BtsI GCAGTG 2 cut(s) 617, 637
BtsIMutI CAGTG 3 cut(s) 617, 637, 749
BveI ACCTGC 1 cut(s) 500
Cfr10I RCCGGY 2 cut(s) 62, 597
Cfr13I GGNCC 1 cut(s) 687
CseI GACGC 1 cut(s) 556
Csp6I GTAC 1 cut(s) 88
CviAII CATG 5 cut(s) 97, 134, 554, 681, 762
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 2 cut(s) 16, 531
DpnI GATC 6 cut(s) 198, 360, 383, 498, 770, 782
DpnII GATC 6 cut(s) 196, 358, 381, 496, 768, 780
DraIII CACNNNGTG 1 cut(s) 728
DrdI GACNNNNNNGTC 1 cut(s) 134
DriI GACNNNNNGTC 1 cut(s) 325
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 614
Eam1105I GACNNNNNGTC 1 cut(s) 325
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 275, 690
Eco147I AGGCCT 1 cut(s) 402
Eco31I GGTCTC 3 cut(s) 259, 333, 349
Eco47I GGWCC 1 cut(s) 687
Eco57I CTGAAG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 687
EcoRII CCWGG 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 275, 690
ErhI CCWWGG 2 cut(s) 275, 690
FaeI CATG 5 cut(s) 100, 137, 557, 684, 765
FaqI GGGAC 1 cut(s) 139
FatI CATG 5 cut(s) 96, 133, 553, 680, 761
FauI CCCGC 1 cut(s) 643
FauNDI CATATG 1 cut(s) 193
Fnu4HI GCNGC 3 cut(s) 290, 293, 296
FokI GGATG 2 cut(s) 41, 659
Fsp4HI GCNGC 3 cut(s) 290, 293, 296
FspBI CTAG 6 cut(s) 44, 200, 276, 506, 725, 784
GluI GCNGC 3 cut(s) 290, 293, 296
HaeIII GGCC 3 cut(s) 402, 408, 616
HapII CCGG 5 cut(s) 63, 286, 452, 479, 598
HgaI GACGC 1 cut(s) 556
Hin1I GRCGYC 2 cut(s) 567, 755
Hin1II CATG 5 cut(s) 100, 137, 557, 684, 765
HincII GTYRAC 2 cut(s) 127, 759
HindII GTYRAC 2 cut(s) 127, 759
HinfI GANTC 5 cut(s) 14, 142, 314, 446, 539
HpaII CCGG 5 cut(s) 63, 286, 452, 479, 598
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 90, 127, 759
Hpy188I TCNGA 7 cut(s) 19, 79, 189, 319, 349, 572, 641
Hpy188III TCNNGA 5 cut(s) 139, 200, 226, 262, 737
Hpy8I GTNNAC 3 cut(s) 90, 127, 759
Hpy99I CGWCG 3 cut(s) 220, 566, 707
HpyAV CCTTC 2 cut(s) 74, 347
HpyCH4IV ACGT 2 cut(s) 705, 755
HpyCH4V TGCA 2 cut(s) 165, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 435, 627
HpyF3I CTNAG 2 cut(s) 16, 531
HpySE526I ACGT 2 cut(s) 705, 755
Hsp92I GRCGYC 2 cut(s) 567, 755
Hsp92II CATG 5 cut(s) 100, 137, 557, 684, 765
Kzo9I GATC 6 cut(s) 196, 358, 381, 496, 768, 780
LmnI GCTCC 4 cut(s) 71, 578, 674, 699
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 3 cut(s) 19, 97, 637
MaeI CTAG 6 cut(s) 44, 200, 276, 506, 725, 784
MaeII ACGT 2 cut(s) 705, 755
MaeIII GTNAC 1 cut(s) 39
MalI GATC 6 cut(s) 198, 360, 383, 498, 770, 782
MboI GATC 6 cut(s) 196, 358, 381, 496, 768, 780
MboII GAAGA 1 cut(s) 725
MfeI CAATTG 1 cut(s) 160
MflI RGATCY 3 cut(s) 381, 496, 780
MhlI GDGCHC 4 cut(s) 51, 62, 440, 751
MlsI TGGCCA 1 cut(s) 616
MluCI AATT 1 cut(s) 160
MluNI TGGCCA 1 cut(s) 616
MlyI GAGTC 4 cut(s) 8, 136, 308, 455
MmeI TCCRAC 1 cut(s) 359
Mox20I TGGCCA 1 cut(s) 616
MscI TGGCCA 1 cut(s) 616
MslI CAYNNNNRTG 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 616
MspI CCGG 5 cut(s) 63, 286, 452, 479, 598
MspR9I CCNGG 2 cut(s) 286, 618
MunI CAATTG 1 cut(s) 160
Mva1269I GAATGC 2 cut(s) 183, 429
MvaI CCWGG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 295, 435, 627
NciI CCSGG 1 cut(s) 286
NdeI CATATG 1 cut(s) 193
NdeII GATC 6 cut(s) 196, 358, 381, 496, 768, 780
NlaIII CATG 5 cut(s) 100, 137, 557, 684, 765
NlaIV GGNNCC 9 cut(s) 61, 67, 237, 383, 437, 498, 580, 670, 688
NmeAIII GCCGAG 1 cut(s) 384
NspI RCATGY 1 cut(s) 100
PceI AGGCCT 1 cut(s) 402
PciI ACATGT 1 cut(s) 96
PctI GAATGC 2 cut(s) 183, 429
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 3 cut(s) 291, 294, 297
PleI GAGTC 4 cut(s) 8, 136, 308, 454
PpsI GAGTC 4 cut(s) 8, 136, 308, 454
PpuMI RGGWCCY 1 cut(s) 687
PscI ACATGT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 9 cut(s) 61, 67, 237, 383, 437, 498, 580, 670, 688
PspPI GGNCC 1 cut(s) 687
PspPPI RGGWCCY 1 cut(s) 687
PsuI RGATCY 3 cut(s) 381, 496, 780
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 95
SatI GCNGC 3 cut(s) 290, 293, 296
Sau3AI GATC 6 cut(s) 196, 358, 381, 496, 768, 780
Sau96I GGNCC 1 cut(s) 687
SchI GAGTC 4 cut(s) 8, 136, 308, 455
ScrFI CCNGG 2 cut(s) 286, 618
SduI GDGCHC 4 cut(s) 51, 62, 440, 751
SetI ASST 8 cut(s) 270, 358, 514, 548, 708, 730, 744, 758
SfaNI GCATC 3 cut(s) 19, 97, 637
SinI GGWCC 1 cut(s) 687
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 1 cut(s) 160
SseBI AGGCCT 1 cut(s) 402
SsiI CCGC 5 cut(s) 69, 239, 289, 292, 650
SspMI CTAG 6 cut(s) 44, 200, 276, 506, 725, 784
StuI AGGCCT 1 cut(s) 402
StyD4I CCNGG 2 cut(s) 284, 616
StyI CCWWGG 2 cut(s) 275, 690
TaiI ACGT 2 cut(s) 708, 758
TaqI TCGA 2 cut(s) 263, 542
TasI AATT 1 cut(s) 160
TatI WGTACW 1 cut(s) 87
TauI GCSGC 2 cut(s) 292, 295
TfiI GAWTC 1 cut(s) 539
TscAI CASTG 3 cut(s) 617, 637, 756
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 3 cut(s) 122, 366, 669
TspGWI ACGGA 2 cut(s) 204, 550
TspRI CASTG 3 cut(s) 617, 637, 756
VpaK11BI GGWCC 1 cut(s) 687
XbaI TCTAGA 1 cut(s) 199
XceI RCATGY 1 cut(s) 100
XmaJI CCTAGG 1 cut(s) 275
XspI CTAG 6 cut(s) 44, 200, 276, 506, 725, 784
ZraI GACGTC 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.