AT4G03030

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002250
Physical Location & Seq
Reverse (-)
1335680 .. 1337565
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G03030.1

Sequence Viewer

Length: 1329 bp
ATGCCTCTGCCTATATTCCCGTCGCGGTTACAGTGTTCGTCTTCATCGTCTTCGTCGTCGTCGCGATCTTCGTTTTTTAGGCCTAAGCTTCGAATCGATCCAAGCTTGACCTTAATTCCAGGCTTATCCAACGACGTCGGGAGACTGATTCTCTCCTTCGTCCCTTACCCTCACATCTCTCGTATCAAATCAACCTGTAAATCATGGTACGCTTTCCTCTCTTCCAAAACCCTAATTTCACTCCGCCATAGCAGAGACAACAGCAACACCAACAATCTCTCGCATCTCCTTTGTATCTTCCCTCAGGATCCTTCGATTTCGCCTCCTTTTCTCTTCGATCCCGTGACTCTCTCTTGGAGATCGCTTCCTCTTATGCCTTGTAACCCTCACGTTTACGGTCTCTGCAATTTCGTAGCTGTAGCTCTTGGTCCTTACGTTTATGTCCTCGGTGGCTCTGCTTTTGATACTAGGTCTTATCCTCTTGATGTTCCTTTGCCTACTTCTTCTGTGTTTCGTTACAGCTTCGTGAAATCGGTTTGGGAACGGTTATCTCCGATGATGTCTCCGCGTGGTAGCTTTGCTTGCGCTGCGATGCCTGGTTCTTGTGGTCGGATTATTGTGGCGGGAGGAGGATCACGGCACACTCTGTTTGGTGCTGCTGGAAGTAGAATGAGTTCTGTGGAGATGTATGATGTTGAGAAAGATGAGTGGAGAGTAATGAATGAATTGCCTAGGTTTAGAGCAGGATGTGTTGGTTTCTTGGTGGAGAATGAGAAGGAGAAGGAGAAGGAGGAGGAAGGGAGAGAGTTTTGGGTTATGGGTGGCTATGGCGGATCGAGGACGGTTTCGGGGATTCTTCCTGTTGATGAGTACTATAAAGACGCGGTAGTGATGGATTTGAGAGTAGATGGTGGTGAGAAATGGAGGGTAGTTGGAGATATGTGGGGAGAAGAAGAGAGACCTAAGCTAGGGAAGATTGTTGCTGTTGATTGTGGGAAGCCTGTGTTTTTCATGTTGGATAAGGATTGGATTCTTAGGTATGAGATGGGTTTGAATCGTTGGAGAAAGGAGTCAAGCGTGCCTAAGAAAGCTCATTACGACAAACCGGTTGGTTTCGTTGCACTGAATGGGGAATTACATGTTATGATTCTTTTGGATGGTTATAATCTGATGGATACAAGGCATACTAGACAACAGAGGAAAGCTGGATCTTTGATGATTCATATGTATGATCCAAAGAAGAAGACTTGGAGATCGGTTGTCTCAAAGCCACCGTTCAATCATCAACTTGATTTCAGGACCACGGTTATGTGCACCATCCGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

442

Amino Acids

49.97

Weight (kDa)

9.29

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_1 PF01344 135 - 187 4.6e-07 Kelch motif
Kelch_1 PF01344 194 - 244 3.9e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AatII GACGTC 1 cut(s) 138
AccII CGCG 4 cut(s) 25, 64, 568, 884
AciI CCGC 6 cut(s) 25, 244, 566, 623, 831, 884
AclWI GGATC 8 cut(s) 92, 302, 315, 332, 640, 841, 1216, 1226
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 2 cut(s) 209, 872
AfiI CCNNNNNNNGG 1 cut(s) 968
AflIII ACRYGT 1 cut(s) 1138
AgeI ACCGGT 1 cut(s) 1105
AgsI TTSAA 2 cut(s) 1054, 1279
AjnI CCWGG 2 cut(s) 118, 595
AluBI AGCT 9 cut(s) 88, 105, 416, 422, 522, 576, 967, 1091, 1205
AluI AGCT 9 cut(s) 88, 105, 416, 422, 522, 576, 967, 1091, 1205
Alw21I GWGCWC 1 cut(s) 1316
Alw26I GTCTC 6 cut(s) 136, 249, 404, 567, 952, 1267
Alw44I GTGCAC 1 cut(s) 1312
AlwI GGATC 8 cut(s) 92, 302, 315, 332, 640, 841, 1216, 1226
AoxI GGCC 1 cut(s) 80
ApaLI GTGCAC 1 cut(s) 1312
ApeKI GCWGC 2 cut(s) 587, 656
AsiGI ACCGGT 1 cut(s) 1105
Asp700I GAANNNNTTC 1 cut(s) 673
AspA2I CCTAGG 1 cut(s) 731
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 2 cut(s) 428, 1299
AsuHPI GGTGA 1 cut(s) 926
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 2 cut(s) 428, 1299
AvrII CCTAGG 1 cut(s) 731
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 1316
BamHI GGATCC 1 cut(s) 307
BbsI GAAGAC 3 cut(s) 33, 42, 1250
Bbv12I GWGCWC 1 cut(s) 1316
BbvI GCAGC 2 cut(s) 574, 643
BccI CCATC 6 cut(s) 886, 902, 1039, 1151, 1165, 1325
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 2 cut(s) 120, 597
BciVI GTATCC 1 cut(s) 1168
BcoDI GTCTC 6 cut(s) 136, 249, 404, 567, 952, 1267
BfaI CTAG 4 cut(s) 468, 732, 968, 1188
BfmI CTRYAG 1 cut(s) 417
BfuI GTATCC 1 cut(s) 1168
BisI GCNGC 2 cut(s) 588, 657
BlnI CCTAGG 1 cut(s) 731
BlsI GCNGC 2 cut(s) 589, 658
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 2 cut(s) 120, 597
Bme18I GGWCC 2 cut(s) 428, 1299
BmgT120I GGNCC 2 cut(s) 428, 1299
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 2 cut(s) 120, 597
BmsI GCATC 2 cut(s) 292, 582
BpiI GAAGAC 3 cut(s) 33, 42, 1250
Bpu10I CCTNAGC 2 cut(s) 84, 963
Bpu14I TTCGAA 1 cut(s) 91
Bsa29I ATCGAT 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 2 cut(s) 404, 952
BsaJI CCNNGG 3 cut(s) 445, 731, 1302
BsaWI WCCGGW 1 cut(s) 1105
Bsc4I CCNNNNNNNGG 1 cut(s) 968
Bse118I RCCGGY 1 cut(s) 1105
Bse21I CCTNAGG 1 cut(s) 303
BseBI CCWGG 2 cut(s) 120, 597
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 3 cut(s) 445, 731, 1302
BseGI GGATG 3 cut(s) 752, 1162, 1317
BseLI CCNNNNNNNGG 1 cut(s) 968
BseMII CTCAG 1 cut(s) 317
BseRI GAGGAG 2 cut(s) 642, 806
BseSI GKGCMC 1 cut(s) 1316
BseXI GCAGC 2 cut(s) 574, 643
Bsh1236I CGCG 4 cut(s) 25, 64, 568, 884
BshFI GGCC 1 cut(s) 82
BshTI ACCGGT 1 cut(s) 1105
BshVI ATCGAT 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 1316
BsiSI CCGG 1 cut(s) 1106
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 968
BsmAI GTCTC 6 cut(s) 136, 249, 404, 567, 952, 1267
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 82
Bso31I GGTCTC 2 cut(s) 404, 952
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 1316
Bsp68I TCGCGA 1 cut(s) 64
BspACI CCGC 6 cut(s) 25, 244, 566, 623, 831, 884
BspANI GGCC 1 cut(s) 82
BspCNI CTCAG 1 cut(s) 316
BspDI ATCGAT 1 cut(s) 96
BspFNI CGCG 4 cut(s) 25, 64, 568, 884
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 8 cut(s) 92, 302, 315, 332, 640, 841, 1216, 1226
BspT104I TTCGAA 1 cut(s) 91
BspTNI GGTCTC 2 cut(s) 404, 952
BsrFI RCCGGY 1 cut(s) 1105
BssAI RCCGGY 1 cut(s) 1105
BssECI CCNNGG 3 cut(s) 445, 731, 1302
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 731
Bst2UI CCWGG 2 cut(s) 120, 597
Bst4CI ACNGT 6 cut(s) 33, 398, 546, 844, 1275, 1306
Bst6I CTCTTC 3 cut(s) 226, 338, 948
BstACI GRCGYC 1 cut(s) 135
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 583, 1079
BstDEI CTNAG 5 cut(s) 84, 303, 963, 1034, 1083
BstDSI CCRYGG 1 cut(s) 1302
BstENI CCTNNNNNAGG 1 cut(s) 966
BstF5I GGATG 3 cut(s) 752, 1162, 1317
BstFNI CGCG 4 cut(s) 25, 64, 568, 884
BstHHI GCGC 1 cut(s) 587
BstMAI GTCTC 6 cut(s) 136, 249, 404, 567, 952, 1267
BstMWI GCNNNNNNNGC 2 cut(s) 582, 587
BstNI CCWGG 2 cut(s) 120, 597
BstNSI RCATGY 1 cut(s) 1142
BstSCI CCNGG 2 cut(s) 118, 595
BstSFI CTRYAG 1 cut(s) 417
BstSLI GKGCMC 1 cut(s) 1316
BstUI CGCG 4 cut(s) 25, 64, 568, 884
BstV1I GCAGC 2 cut(s) 574, 643
BstV2I GAAGAC 3 cut(s) 33, 42, 1250
BstX2I RGATCY 2 cut(s) 307, 1208
BstYI RGATCY 2 cut(s) 307, 1208
Bsu15I ATCGAT 1 cut(s) 96
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 1 cut(s) 82
BsuTUI ATCGAT 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 1302
BtgZI GCGATG 1 cut(s) 605
BtsCI GGATG 3 cut(s) 752, 1162, 1317
BtsIMutI CAGTG 2 cut(s) 38, 1121
BtuMI TCGCGA 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 583, 1079
CfoI GCGC 1 cut(s) 587
Cfr10I RCCGGY 1 cut(s) 1105
Cfr13I GGNCC 2 cut(s) 428, 1299
ClaI ATCGAT 1 cut(s) 96
CseI GACGC 1 cut(s) 890
Csp6I GTAC 2 cut(s) 208, 871
CspAI ACCGGT 1 cut(s) 1105
CviAII CATG 3 cut(s) 204, 1012, 1139
CviQI GTAC 2 cut(s) 208, 871
DdeI CTNAG 5 cut(s) 84, 303, 963, 1034, 1083
Eam1104I CTCTTC 3 cut(s) 226, 338, 948
EarI CTCTTC 3 cut(s) 226, 338, 948
EciI GGCGGA 2 cut(s) 233, 846
Eco130I CCWWGG 1 cut(s) 731
Eco147I AGGCCT 1 cut(s) 82
Eco31I GGTCTC 2 cut(s) 404, 952
Eco47I GGWCC 2 cut(s) 428, 1299
Eco81I CCTNAGG 1 cut(s) 303
EcoNI CCTNNNNNAGG 1 cut(s) 966
EcoRII CCWGG 2 cut(s) 118, 595
EcoT14I CCWWGG 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 731
FaeI CATG 3 cut(s) 207, 1015, 1142
FaqI GGGAC 1 cut(s) 146
FatI CATG 3 cut(s) 203, 1011, 1138
FauI CCCGC 1 cut(s) 616
FauNDI CATATG 1 cut(s) 1224
Fnu4HI GCNGC 2 cut(s) 588, 657
FokI GGATG 3 cut(s) 759, 1169, 1304
Fsp4HI GCNGC 2 cut(s) 588, 657
FspBI CTAG 4 cut(s) 468, 732, 968, 1188
GlaI GCGC 1 cut(s) 586
GluI GCNGC 2 cut(s) 588, 657
HaeIII GGCC 1 cut(s) 82
HapII CCGG 1 cut(s) 1106
HgaI GACGC 1 cut(s) 890
HhaI GCGC 1 cut(s) 587
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 3 cut(s) 207, 1015, 1142
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HindIII AAGCTT 2 cut(s) 86, 103
HinfI GANTC 9 cut(s) 93, 148, 346, 853, 1030, 1054, 1070, 1147, 1219
HpaII CCGG 1 cut(s) 1106
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 2 cut(s) 394, 1314
Hpy188I TCNGA 4 cut(s) 555, 612, 1170, 1322
Hpy188III TCNNGA 6 cut(s) 63, 139, 305, 482, 526, 1297
Hpy8I GTNNAC 2 cut(s) 394, 1314
Hpy99I CGWCG 6 cut(s) 25, 58, 61, 64, 137, 140
HpyAV CCTTC 6 cut(s) 166, 321, 769, 775, 781, 791
HpyCH4III ACNGT 6 cut(s) 33, 398, 546, 844, 1275, 1306
HpyCH4IV ACGT 3 cut(s) 135, 390, 435
HpyCH4V TGCA 3 cut(s) 405, 1121, 1314
HpyF10VI GCNNNNNNNGC 2 cut(s) 582, 587
HpyF3I CTNAG 5 cut(s) 84, 303, 963, 1034, 1083
HpySE526I ACGT 3 cut(s) 135, 390, 435
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 3 cut(s) 207, 1015, 1142
HspAI GCGC 1 cut(s) 585
Lsp1109I GCAGC 2 cut(s) 574, 643
LweI GCATC 2 cut(s) 292, 582
MaeI CTAG 4 cut(s) 468, 732, 968, 1188
MaeII ACGT 3 cut(s) 135, 390, 435
MaeIII GTNAC 4 cut(s) 27, 343, 380, 515
MflI RGATCY 2 cut(s) 307, 1208
MhlI GDGCHC 1 cut(s) 1316
MluCI AATT 5 cut(s) 114, 234, 406, 725, 1133
MlyI GAGTC 2 cut(s) 340, 1079
MmeI TCCRAC 5 cut(s) 153, 590, 913, 996, 1040
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 2 cut(s) 1227, 1307
MspI CCGG 1 cut(s) 1106
MspR9I CCNGG 2 cut(s) 120, 597
MvaI CCWGG 2 cut(s) 120, 597
MvnI CGCG 4 cut(s) 25, 64, 568, 884
MwoI GCNNNNNNNGC 2 cut(s) 582, 587
NdeI CATATG 1 cut(s) 1224
NlaIII CATG 3 cut(s) 207, 1015, 1142
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 343
NruI TCGCGA 1 cut(s) 64
NspI RCATGY 1 cut(s) 1142
NspV TTCGAA 1 cut(s) 91
PceI AGGCCT 1 cut(s) 82
PciI ACATGT 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 3 cut(s) 44, 53, 68
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 7 cut(s) 93, 148, 853, 1030, 1054, 1147, 1219
PinAI ACCGGT 1 cut(s) 1105
PkrI GCNGC 2 cut(s) 589, 658
PleI GAGTC 2 cut(s) 340, 1078
PpsI GAGTC 2 cut(s) 340, 1078
PscI ACATGT 1 cut(s) 1138
PsiI TTATAA 1 cut(s) 1164
Psp6I CCWGG 2 cut(s) 118, 595
PspGI CCWGG 2 cut(s) 118, 595
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 428, 1299
PsrI GAACNNNNNNTAC 2 cut(s) 658, 690
PsuI RGATCY 2 cut(s) 307, 1208
RruI TCGCGA 1 cut(s) 64
RsaI GTAC 2 cut(s) 209, 872
RsaNI GTAC 2 cut(s) 208, 871
RseI CAYNNNNRTG 2 cut(s) 1227, 1307
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 2 cut(s) 588, 657
Sau96I GGNCC 2 cut(s) 428, 1299
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 2 cut(s) 340, 1079
ScrFI CCNGG 2 cut(s) 120, 597
SduI GDGCHC 1 cut(s) 1316
SfaNI GCATC 2 cut(s) 292, 582
SfcI CTRYAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 2 cut(s) 428, 1299
SmiMI CAYNNNNRTG 2 cut(s) 1227, 1307
Sse9I AATT 5 cut(s) 114, 234, 406, 725, 1133
SseBI AGGCCT 1 cut(s) 82
SsiI CCGC 6 cut(s) 25, 244, 566, 623, 831, 884
SspMI CTAG 4 cut(s) 468, 732, 968, 1188
StuI AGGCCT 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 118, 595
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 6 cut(s) 33, 398, 546, 844, 1275, 1306
TaiI ACGT 3 cut(s) 138, 393, 438
TaqI TCGA 5 cut(s) 91, 96, 314, 336, 836
TasI AATT 5 cut(s) 114, 234, 406, 725, 1133
TatI WGTACW 1 cut(s) 870
TfiI GAWTC 7 cut(s) 93, 148, 853, 1030, 1054, 1147, 1219
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 2 cut(s) 38, 1128
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 2 cut(s) 587, 656
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 5 cut(s) 33, 734, 738, 1000, 1211
TspRI CASTG 2 cut(s) 38, 1128
VneI GTGCAC 1 cut(s) 1312
VpaK11BI GGWCC 2 cut(s) 428, 1299
XagI CCTNNNNNAGG 1 cut(s) 966
XceI RCATGY 1 cut(s) 1142
XmaJI CCTAGG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 4 cut(s) 468, 732, 968, 1188
ZraI GACGTC 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.