Rorug06G0199300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002250
Physical Location & Seq
Forward (+)
32045910 .. 32048100
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0199300.1

Sequence Viewer

Length: 1497 bp
ATGGCTTTCATTTGGGTGATAGTTGGATTGCTTGCACTTATTTACGTCCTGCAACCCTGGTCATGGAGAAGCAAGAAGCGGTTGCCTCCTGGTCCGAGAGGGTTTCCCATTTTCGGAAGCCTCAACTTGCTAGGGAAGTTCCCTCACAAAGATCTGCATCAACTGGCCCGAAAGTACGGTGACATCATGTACATGCGGTTAGGCCTTGTGCCTGTCATTGTCGTCTCTTCCCCTCGAGCAGCCGAGCTGTTCCTCAAAACCCACGACCTTGTGTTTGCGAGCAGGCCGCCTCACGAAGGTTCAAAGCATATTTCTTATGGCCAAAGGAACTTGACTCTTGCTGAGTATGGCTCTTACTGGCGTGACATGCGCAAGATGTGCACTCTTGAGTTACTAAGCAACCACAAAATCAACTCCTTCAAGTCAATGAGGAAAGAAGAGGTCGCCCTCATGGTGGATTCTATTCGAGAGTCTGCAGCCAAGGACCAACGTGTTTCGGTTGATCTGAGCTCCAAGGTGTCATCTCTTAGCGCAGACATGACTTGCAGGATGGTGTTTGGGAAGAAATACAAGGATGAGGAATTTAGTGAGAGGGGTTTTAAATCCGTGATTCAAGAAGGCTTGCAATTAGGAGCTGCCCCTAACTTGGGAGATTACATTCCTTGCATTGCTCCACTTGATCTCCAAGGATTCACTAAACGAATGAAGGCTGTTCACAAGGTCTTTGATGACTTTTTGGAGAAGATTGTGGAGGAGCATCTTCAATCTAGGGATCATGGAGAAAGAAGAACCAAGGACTTTGTTGATGTCATGCTGGGCTTCATGGAGTCTGAAGAATCTGCGTACCAAATCGGACGCTCAAACATCAAAGCCATCATATTGGACATGCTCACCGCCTCAATGGACACATCAGCAACAACAATTGAGTGGGCACTCTCCGAACTCATCAGGCATCCAGAGGTAATCAAGAAAGTGCAAATAGAGTTAGCAAATGTAGTCGGAATGGAGAGGATGGTGGAGGAGTCAGACTTGGAGAAGTTGGAGTACATGGACATGGTAGTGAAGGAAAACTTGAGGCTACATCCGGTAGCACCATTATTGCTTCCCCATCAATCAACTGAAGATTGCACTGTTAATGGCTTCCACATACCCAAAAAGTCGCGCATTATGATAAATGTATGGTCGATTGGGAGAGACCCAAATGCTTGGACTGAAGCAGAGAAGTTCATACCTGAGAGGTTCGTGGATAGTAGTATAGATCTTCGGGGAAACCACTTTGAGCTTCTTCCCTTTGGCTCTGGACGAAGGCGTTGCCCTGGAATTCAACTAGGTCTAACGGTTGTCCAGTTAGTGCTGGCGCAACTTGTGCATTGTTTTGATTGGGAACTTCCAGAAAACATGTCACCGGATGATTTGGACATGACGGAAGAATTTGGAATAACGGTTCCTAGGGCCAAGCATCTACTGGCAATTCCAGCATATCGCCTTCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

498

Amino Acids

56.87

Weight (kDa)

7.0

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 29 - 487 4.6e-109 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 371
AccII CGCG 1 cut(s) 1162
AciI CCGC 4 cut(s) 79, 196, 287, 894
AclWI GGATC 1 cut(s) 780
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 3 cut(s) 581, 1320, 1430
AcuI CTGAAG 3 cut(s) 852, 1140, 1233
AfaI GTAC 4 cut(s) 176, 191, 845, 1046
AfiI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
AflIII ACRYGT 2 cut(s) 490, 1398
AgsI TTSAA 5 cut(s) 303, 421, 614, 764, 1325
AjnI CCWGG 3 cut(s) 56, 88, 1315
AloI GAACNNNNNNTCC 2 cut(s) 1428, 1460
AluBI AGCT 4 cut(s) 247, 510, 635, 1282
AluI AGCT 4 cut(s) 247, 510, 635, 1282
Alw21I GWGCWC 2 cut(s) 383, 512
Alw26I GTCTC 2 cut(s) 229, 1188
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 1 cut(s) 780
Ama87I CYCGRG 1 cut(s) 234
AoxI GGCC 5 cut(s) 165, 202, 284, 319, 1452
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 3 cut(s) 239, 476, 635
ApoI RAATTY 3 cut(s) 581, 1320, 1430
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspA2I CCTAGG 1 cut(s) 1448
AspLEI GCGC 4 cut(s) 372, 533, 1164, 1360
AspS9I GGNCC 4 cut(s) 92, 166, 484, 1452
AsuHPI GGTGA 4 cut(s) 28, 191, 883, 1395
AvaI CYCGRG 1 cut(s) 234
AvaII GGWCC 2 cut(s) 92, 484
AvrII CCTAGG 1 cut(s) 1448
BaeGI GKGCMC 2 cut(s) 383, 934
BalI TGGCCA 1 cut(s) 321
BanII GRGCYC 1 cut(s) 512
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
Bbv12I GWGCWC 2 cut(s) 383, 512
BbvI GCAGC 3 cut(s) 251, 488, 622
BccI CCATC 4 cut(s) 544, 881, 1006, 1116
BcgI CGANNNNNNTGC 2 cut(s) 1293, 1327
BciT130I CCWGG 3 cut(s) 58, 90, 1317
BcoDI GTCTC 2 cut(s) 229, 1188
BfaI CTAG 4 cut(s) 131, 768, 1328, 1449
BfmI CTRYAG 1 cut(s) 474
BglII AGATCT 2 cut(s) 151, 1258
BisI GCNGC 4 cut(s) 240, 287, 477, 636
BlnI CCTAGG 1 cut(s) 1448
BlsI GCNGC 4 cut(s) 241, 288, 478, 637
Bme1390I CCNGG 3 cut(s) 58, 90, 1317
Bme18I GGWCC 2 cut(s) 92, 484
BmeT110I CYCGRG 1 cut(s) 234
BmgT120I GGNCC 4 cut(s) 92, 166, 484, 1452
BmiI GGNNCC 1 cut(s) 1446
BmrFI CCNGG 3 cut(s) 58, 90, 1317
BmsI GCATC 4 cut(s) 166, 766, 961, 1468
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpuEI CTTGAG 2 cut(s) 407, 1093
BsaBI GATNNNNATC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 1188
BsaJI CCNNGG 7 cut(s) 56, 480, 513, 685, 792, 1315, 1448
BsaWI WCCGGW 2 cut(s) 1084, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
Bse1I ACTGG 4 cut(s) 168, 362, 1345, 1470
Bse3DI GCAATG 1 cut(s) 666
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 3 cut(s) 58, 90, 1317
BseDI CCNNGG 7 cut(s) 56, 480, 513, 685, 792, 1315, 1448
BseGI GGATG 6 cut(s) 555, 580, 952, 1017, 1081, 1414
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 3 cut(s) 333, 497, 1224
BseNI ACTGG 4 cut(s) 168, 362, 1345, 1470
BseRI GAGGAG 2 cut(s) 767, 1034
BseSI GKGCMC 2 cut(s) 383, 934
BseXI GCAGC 3 cut(s) 251, 488, 622
BseYI CCCAGC 1 cut(s) 814
Bsh1236I CGCG 1 cut(s) 1162
BshFI GGCC 5 cut(s) 167, 204, 286, 321, 1454
BsiHKAI GWGCWC 2 cut(s) 383, 512
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 2 cut(s) 1085, 1406
BslI CCNNNNNNNGG 6 cut(s) 63, 113, 296, 454, 646, 647
BsmAI GTCTC 2 cut(s) 229, 1188
BsmBI CGTCTC 1 cut(s) 229
BsnI GGCC 5 cut(s) 167, 204, 286, 321, 1454
Bso31I GGTCTC 1 cut(s) 1188
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 3 cut(s) 383, 512, 934
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 5 cut(s) 151, 502, 679, 772, 1258
BspACI CCGC 4 cut(s) 79, 196, 287, 894
BspANI GGCC 5 cut(s) 167, 204, 286, 321, 1454
BspCNI CTCAG 3 cut(s) 334, 498, 1225
BspFNI CGCG 1 cut(s) 1162
BspLI GGNNCC 1 cut(s) 1446
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 780
BspTNI GGTCTC 1 cut(s) 1188
BsrDI GCAATG 1 cut(s) 666
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 4 cut(s) 168, 362, 1345, 1470
BssECI CCNNGG 7 cut(s) 56, 480, 513, 685, 792, 1315, 1448
BssMI GATC 5 cut(s) 151, 502, 679, 772, 1258
BssT1I CCWWGG 5 cut(s) 480, 513, 685, 792, 1448
Bst2UI CCWGG 3 cut(s) 58, 90, 1317
Bst4CI ACNGT 4 cut(s) 179, 1132, 1339, 1444
Bst6I CTCTTC 2 cut(s) 232, 432
BstAPI GCANNNNNTGC 2 cut(s) 378, 1366
BstAUI TGTACA 1 cut(s) 189
BstC8I GCNNGC 5 cut(s) 33, 280, 284, 623, 1356
BstDEI CTNAG 5 cut(s) 342, 395, 506, 527, 1233
BstENI CCTNNNNNAGG 1 cut(s) 294
BstF5I GGATG 6 cut(s) 555, 580, 952, 1017, 1081, 1414
BstFNI CGCG 1 cut(s) 1162
BstHHI GCGC 4 cut(s) 372, 533, 1164, 1360
BstKTI GATC 5 cut(s) 154, 505, 682, 775, 1261
BstMAI GTCTC 2 cut(s) 229, 1188
BstMBI GATC 5 cut(s) 151, 502, 679, 772, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 367, 378, 1366, 1475
BstNI CCWGG 3 cut(s) 58, 90, 1317
BstNSI RCATGY 4 cut(s) 196, 370, 889, 1402
BstSCI CCNGG 3 cut(s) 56, 88, 1315
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 2 cut(s) 383, 934
BstUI CGCG 1 cut(s) 1162
BstV1I GCAGC 3 cut(s) 251, 488, 622
BstX2I RGATCY 2 cut(s) 151, 1258
BstXI CCANNNNNNTGG 2 cut(s) 880, 1206
BstYI RGATCY 2 cut(s) 151, 1258
BsuRI GGCC 5 cut(s) 167, 204, 286, 321, 1454
BtsCI GGATG 6 cut(s) 555, 580, 952, 1017, 1081, 1414
BtsIMutI CAGTG 1 cut(s) 1128
Cac8I GCNNGC 5 cut(s) 33, 280, 284, 623, 1356
CfoI GCGC 4 cut(s) 372, 533, 1164, 1360
Cfr13I GGNCC 4 cut(s) 92, 166, 484, 1452
CseI GACGC 1 cut(s) 864
Csp6I GTAC 4 cut(s) 175, 190, 844, 1045
CviQI GTAC 4 cut(s) 175, 190, 844, 1045
DdeI CTNAG 5 cut(s) 342, 395, 506, 527, 1233
DpnI GATC 5 cut(s) 153, 504, 681, 774, 1260
DpnII GATC 5 cut(s) 151, 502, 679, 772, 1258
DraI TTTAAA 1 cut(s) 601
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 232, 432
EarI CTCTTC 2 cut(s) 232, 432
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 5 cut(s) 480, 513, 685, 792, 1448
Eco147I AGGCCT 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 512
Eco31I GGTCTC 1 cut(s) 1188
Eco47I GGWCC 2 cut(s) 92, 484
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 3 cut(s) 852, 1140, 1233
Eco88I CYCGRG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 510
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 1320
EcoRII CCWGG 3 cut(s) 56, 88, 1315
EcoT14I CCWWGG 5 cut(s) 480, 513, 685, 792, 1448
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 5 cut(s) 480, 513, 685, 792, 1448
Esp3I CGTCTC 1 cut(s) 229
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
Fnu4HI GCNGC 4 cut(s) 240, 287, 477, 636
FokI GGATG 6 cut(s) 562, 587, 939, 1024, 1068, 1421
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 4 cut(s) 240, 287, 477, 636
FspBI CTAG 4 cut(s) 131, 768, 1328, 1449
FspI TGCGCA 1 cut(s) 371
GlaI GCGC 4 cut(s) 371, 532, 1163, 1359
GluI GCNGC 4 cut(s) 240, 287, 477, 636
GsaI CCCAGC 1 cut(s) 818
HaeIII GGCC 5 cut(s) 167, 204, 286, 321, 1454
HapII CCGG 2 cut(s) 1085, 1406
HgaI GACGC 1 cut(s) 864
HhaI GCGC 4 cut(s) 372, 533, 1164, 1360
Hin6I GCGC 4 cut(s) 370, 531, 1162, 1358
HinP1I GCGC 4 cut(s) 370, 531, 1162, 1358
HinfI GANTC 8 cut(s) 334, 458, 470, 610, 690, 827, 836, 1022
HpaII CCGG 2 cut(s) 1085, 1406
HphI GGTGA 4 cut(s) 28, 191, 883, 1395
Hpy166II GTNNAC 2 cut(s) 381, 715
Hpy188I TCNGA 8 cut(s) 96, 116, 507, 832, 854, 940, 1001, 1027
Hpy188III TCNNGA 8 cut(s) 293, 386, 467, 614, 956, 967, 1299, 1391
Hpy8I GTNNAC 2 cut(s) 381, 715
HpyAV CCTTC 7 cut(s) 290, 427, 611, 700, 1057, 1299, 1496
HpyCH4III ACNGT 4 cut(s) 179, 1132, 1339, 1444
HpyCH4IV ACGT 2 cut(s) 45, 490
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 378, 1366, 1475
HpyF3I CTNAG 5 cut(s) 342, 395, 506, 527, 1233
HpySE526I ACGT 2 cut(s) 45, 490
HspAI GCGC 4 cut(s) 370, 531, 1162, 1358
Kzo9I GATC 5 cut(s) 151, 502, 679, 772, 1258
LmnI GCTCC 4 cut(s) 515, 632, 676, 754
Lsp1109I GCAGC 3 cut(s) 251, 488, 622
LweI GCATC 4 cut(s) 166, 766, 961, 1468
MaeI CTAG 4 cut(s) 131, 768, 1328, 1449
MaeII ACGT 2 cut(s) 45, 490
MaeIII GTNAC 4 cut(s) 179, 362, 390, 1401
MalI GATC 5 cut(s) 153, 504, 681, 774, 1260
MboI GATC 5 cut(s) 151, 502, 679, 772, 1258
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 2 cut(s) 151, 1258
MhlI GDGCHC 3 cut(s) 383, 512, 934
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 6 cut(s) 581, 626, 921, 1320, 1430, 1470
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 4 cut(s) 328, 479, 836, 1031
MmeI TCCRAC 3 cut(s) 4, 979, 1020
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 2 cut(s) 600, 1134
MslI CAYNNNNRTG 4 cut(s) 14, 191, 1052, 1058
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 2 cut(s) 1085, 1406
MspR9I CCNGG 3 cut(s) 58, 90, 1317
MunI CAATTG 1 cut(s) 921
MvaI CCWGG 3 cut(s) 58, 90, 1317
MvnI CGCG 1 cut(s) 1162
MwoI GCNNNNNNNGC 4 cut(s) 367, 378, 1366, 1475
NdeII GATC 5 cut(s) 151, 502, 679, 772, 1258
NlaIV GGNNCC 1 cut(s) 1446
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 3 cut(s) 179, 362, 1401
NsbI TGCGCA 1 cut(s) 371
NspI RCATGY 4 cut(s) 196, 370, 889, 1402
PaeR7I CTCGAG 1 cut(s) 234
PceI AGGCCT 1 cut(s) 204
PciI ACATGT 1 cut(s) 1398
PfeI GAWTC 4 cut(s) 458, 610, 690, 836
PkrI GCNGC 4 cut(s) 241, 288, 478, 637
PleI GAGTC 4 cut(s) 328, 478, 835, 1030
PpsI GAGTC 4 cut(s) 328, 478, 835, 1030
PscI ACATGT 1 cut(s) 1398
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 56, 88, 1315
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 3 cut(s) 56, 88, 1315
PspN4I GGNNCC 1 cut(s) 1446
PspPI GGNCC 4 cut(s) 92, 166, 484, 1452
PspXI VCTCGAGB 1 cut(s) 234
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 2 cut(s) 151, 1258
RsaI GTAC 4 cut(s) 176, 191, 845, 1046
RsaNI GTAC 4 cut(s) 175, 190, 844, 1045
RseI CAYNNNNRTG 4 cut(s) 14, 191, 1052, 1058
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 2 cut(s) 600, 1134
SatI GCNGC 4 cut(s) 240, 287, 477, 636
Sau3AI GATC 5 cut(s) 151, 502, 679, 772, 1258
Sau96I GGNCC 4 cut(s) 92, 166, 484, 1452
SchI GAGTC 4 cut(s) 328, 479, 836, 1031
ScrFI CCNGG 3 cut(s) 58, 90, 1317
SduI GDGCHC 3 cut(s) 383, 512, 934
SfaNI GCATC 4 cut(s) 166, 766, 961, 1468
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 234
SinI GGWCC 2 cut(s) 92, 484
SlaI CTCGAG 1 cut(s) 234
SmiMI CAYNNNNRTG 4 cut(s) 14, 191, 1052, 1058
SmlI CTYRAG 3 cut(s) 234, 386, 1072
SmoI CTYRAG 3 cut(s) 234, 386, 1072
Sse9I AATT 6 cut(s) 581, 626, 921, 1320, 1430, 1470
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 4 cut(s) 79, 196, 287, 894
SspMI CTAG 4 cut(s) 131, 768, 1328, 1449
SstI GAGCTC 1 cut(s) 512
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 3 cut(s) 56, 88, 1315
StyI CCWWGG 5 cut(s) 480, 513, 685, 792, 1448
TaaI ACNGT 4 cut(s) 179, 1132, 1339, 1444
TaiI ACGT 2 cut(s) 48, 493
TaqI TCGA 3 cut(s) 235, 466, 1184
TasI AATT 6 cut(s) 581, 626, 921, 1320, 1430, 1470
TatI WGTACW 2 cut(s) 189, 1044
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 4 cut(s) 458, 610, 690, 836
Tru1I TTAA 2 cut(s) 600, 1134
Tru9I TTAA 2 cut(s) 600, 1134
TscAI CASTG 1 cut(s) 1135
TseFI GTSAC 3 cut(s) 179, 362, 1401
TseI GCWGC 3 cut(s) 239, 476, 635
Tsp45I GTSAC 3 cut(s) 179, 362, 1401
TspDTI ATGAA 3 cut(s) 719, 811, 1216
TspGWI ACGGA 2 cut(s) 595, 1439
TspRI CASTG 1 cut(s) 1135
VneI GTGCAC 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 92, 484
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 3 cut(s) 581, 1320, 1430
XceI RCATGY 4 cut(s) 196, 370, 889, 1402
XcmI CCANNNNNNNNNTGG 1 cut(s) 1462
XhoI CTCGAG 1 cut(s) 234
XmaJI CCTAGG 1 cut(s) 1448
XspI CTAG 4 cut(s) 131, 768, 1328, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.